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Plasmid#49240Purposeexpresses human Med12 with His tag in insect cellsDepositorInsertMed12 (MED12 Human)
UseTagsHisExpressionInsectMutationPromoterPolyhedrinAvailable sinceNov. 6, 2013AvailabilityAcademic Institutions and Nonprofits only -
pDEST-HemmarR
Plasmid#31222DepositorInsertCD4-tdTom (CD4 Human, Aequorea victoria, Fly)
UseTagsCD4 and tdTomatoExpressionInsectMutationFusion of signal peptide of Drosophila Akh gene, …PromoterAvailable sinceOct. 5, 2011AvailabilityAcademic Institutions and Nonprofits only -
pOPINM
Plasmid#26044DepositorInsertHis-MBP-3C-lacZ
UseTagsMAHHHHHHSSG_MBP_LEVLFQGPExpressionBacterial, Insect, and Mamm…MutationPromoterAvailable sinceJune 30, 2011AvailabilityAcademic Institutions and Nonprofits only -
pBac[3xP3-DsRed; UAS-Tc'hsp_p-tGFP-SV40]
Plasmid#86453PurposeUAS responder construct for misexpression in Tribolium; UAS drives tGFP expression; (Transformation plasmid; Red eye marker)DepositorInsert3xP3-DsRed; UAS-Tc'hsp_p-tGFP-SV40
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pOPINJ
Plasmid#26045DepositorInsertHisGST3C-lacZ
UseTagsMAHHHHHH_GST_LEVLFQGTExpressionBacterial, Insect, and Mamm…MutationPromoterAvailable sinceOct. 19, 2011AvailabilityAcademic Institutions and Nonprofits only -
His-ZZ-TEV-SNAPf DHC1_IC2C_LIC2_Tctex1_Robl1_LC8
Plasmid#111903PurposeFull length human cytoplasmic dynein 1 complex, optimised for Sf9 expression. 8xHis-ZZ tag followed by TEV cleavage site and SNAPf tag on the N-terminus of dynein heavy chain 1.DepositorInsertsUseTags8xHis, SNAPf-tag, TEV site, and ZZ-tagExpressionInsectMutationOptimised for expression in Sf9 cells.PromoterAvailable sinceJuly 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFastBac His6 TEV LIC cloning vector (4B)
Plasmid#30115DepositorTypeEmpty backboneUseTagsHis6-TEVExpressionInsectMutationPromoterAvailable sinceJune 9, 2011AvailabilityAcademic Institutions and Nonprofits only -
pMT692 ORF2p-3C-3xF (1-1275, ORFeus-Hs) in pDARMO-PolH2.1
Plasmid#213025PurposeExpresses full length human LINE-1 ORF2p Core (1-1275) with a C-terminal 3xFlag tag for baculovirus production in insect cellsDepositorInsertLINE-1 ORF2p (1-1275)
UseTags3C-3xFlag (ORF2p)ExpressionInsectMutationPromoterPolyhedrinAvailable sinceMay 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
TH1204-pOCC120-FUS_opt(NheI)
Plasmid#221892PurposeShuttle vector for baculovirus production, using FlashBac bacmidDepositorInsertFUS (FUS Human)
UseTagsMBP-PreScission and mGFP-PreScission-6xHisExpressionInsectMutationwtPromoterPH promoterAvailable sinceJuly 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFastBac-M13-6His-KIF1C-GFP
Plasmid#130975PurposeExpression of 6His-KIF1C-GFP in insect cells for protein purification.DepositorInsertKIF1C-GFP (KIF1C Human)
UseTags6His and GFPExpressionInsectMutation5 silent mutations that make this construct RNAi …PromoterPolyhedrinAvailable sinceOct. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCRISPaint-T2A-Gal4-3xP3-RFP
Plasmid#127556PurposeCRISPaint universal donor plasmid for gene exon insertion. Encodes T2A-Gal4-SV40 and 3xP3-RFP visible eye marker.DepositorInsertGal4
UseCRISPRTagsExpressionInsectMutationPromoterAvailable sinceAug. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAct:dCas9-VPR
Plasmid#78898PurposeExpresses dCas9-VPR under Actin promoter for CRISPRa in Drosophila cell culture. Please note- this plasmid does not express GFP.DepositorInsertdCas9-VPR
UseCRISPRTagsExpressionInsectMutationPromoterpActin (Drosophila)Available sinceAug. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBPGAL4.2::VP16Uw
Plasmid#26228DepositorInsertGAL4::VP16 (GAL4 Budding Yeast)
UseTagsExpressionInsectMutationPromoterAvailable sinceOct. 7, 2010AvailabilityAcademic Institutions and Nonprofits only -
pTriEx-mCherry-Zdk1
Plasmid#81057Purposemammalian expression of Zdk1DepositorInsertZdk1
UseTags6x His tag, FLAG, and mCherryExpressionBacterial, Insect, and Mamm…MutationPromoterAvailable sinceAug. 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
pIDK-HDAC1fl
Plasmid#109158PurposeEncodes human full-length HDAC1 to be expressed in a baculovirus/insect cell expression systemDepositorInserthuman full-length HDAC1, sequence-optimized for insect cells (HDAC1 Human)
UseTagsExpressionInsectMutationPromoterp10Available sinceJan. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pIZ-Flag6His-BmAgo3
Plasmid#50559PurposeExpresses FLAG, His tagged BmAgo3 in silkworm cell line BmN4 or insect cellsDepositorInsertBmAgo3
UseTags6xHIS and FlagExpressionInsectMutationPromoterOpIE2Available sinceFeb. 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pRL-ubi63E
Plasmid#74280PurposeRenilla control plasmid driven by the ubiquitin 63E promoterDepositorInsertRenilla luciferase
UseTagsExpressionInsectMutationPromoterubiquitin 63E promoterAvailable sinceApril 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
Tubp-Gal80 in pCaSpeR4
Plasmid#17748DepositorInsertGAL80 (GAL80 Budding Yeast)
UseTagsSV40 polyA and Tubulin 1alpha promoterExpressionInsectMutationPromoterAvailable sinceMay 14, 2008AvailabilityAcademic Institutions and Nonprofits only -
pUASTLOTattB_VHH-GFP4::CD8::mCherry
Plasmid#163917PurposeExpression of a membrane-tethered GFP-nanobody marked by mCherry under control of the UAS or LOP enhancersDepositorInsertFusion of vhhGPF4 nanobody with mouse CD8 transmembrane protein and mCherry fluorophor
UseCre/LoxTagsmCherryExpressionInsectMutationPromoterAvailable sinceJan. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKSB- 3xP3-YFPsv40 GGGG AAGA
Plasmid#174530Purpose3xP3-YFP fluorescence marker cassette for gene knock-in in mosquitoesDepositorInsert3xP3-YFP BsaI cassette
UseTagsExpressionInsectMutationPromoter3xP3Available sinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only