4,908 results

1. pIDS-RESTwt full-length

   Plasmid

   #109161

   Purpose

   Encodes human full-length REST/NRSF to be expressed in a baculovirus/insect cell expression system

   Depositor

   Monika Golas

   Article

   Inui et al Protein Sci. 2017 May;26(5):997-1011. do

   Insert

   human full-length REST/NRSF (REST Human)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   p10

   Available since

   Jan. 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. p(U6a-BsaI-gRNA)

   Plasmid

   #65955

   Purpose

   Tribolium U6a promoter with BsaI cloning site to insert guide RNA sequence

   Depositor

   Michalis Averof

   Article

   Gilles et al Development. 2015 Jul 9. pii: dev.125054

   Type

   Empty backbone

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   U6

   Available since

   Aug. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pFL

   Plasmid

   #110739

   Purpose

   Plasmid for the insect cell expression system, acceptor for plasmid fusions, two promoters, Multiplication Module M, Tn7 transposition and LoxP site

   Depositor

   Tim Richmond

   Article

   Fitzgerald et al Nat Methods. 2006 Dec;3(12):1021-32. doi

   Type

   Empty backbone

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Polyhedrin and p10 promoters

   Available since

   June 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pACEBac1-PARP2-Flag-His-WT

   Plasmid

   #111574

   Purpose

   Expresses full length PARP2 in Sf9 (insect) cells

   Depositor

   Thomas Muir

   Article

   Liszczak et al Nat Chem Biol. 2018 Jul 16. pii: 10.1038

   Insert

   PARP2 (PARP2 Human)
   

   Use

   Tags

   C-terminal 6xHis and C-terminal Flag

   Expression

   Insect

   Mutation

   Promoter

   polyhedrin promoter

   Available since

   July 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pFastBac His6 TEV LIC cloning vector (4B)

   Plasmid

   #30115

   Depositor

   Scott Gradia

   Type

   Empty backbone

   Use

   Tags

   His6-TEV

   Expression

   Insect

   Mutation

   Promoter

   Available since

   June 9, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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6. pExpreS2-1-C3-PA-10H-SIII

   Plasmid

   #175443

   Purpose

   HiFi-compatible destination vector for secreted overexpression of 3C-cleavable, C-terminal Protein A-10His-TwinStrep tagged proteins with ExpreS2 Platform

   Depositor

   Wyatt Yue

   Article

   Coker et al Front Bioeng Biotechnol. 2022 May 5;10:8

   Type

   Empty backbone

   Use

   Tags

   3C-Protein A-10His-TwinStrep and BiP Secretion Pe…

   Expression

   Insect

   Mutation

   Promoter

   Fused Actin-HSP70

   Available since

   April 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pQUAST

   Plasmid

   #24349

   Depositor

   Liqun Luo

   Article

   Potter et al Cell. 2010 Apr 30. 141(3):536-48.

   Insert

   5xQUAS-Pmin promoter
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   April 12, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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8. pOCC5

   Plasmid

   #118863

   Purpose

   shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6 tag, cleavable with 3C protease

   Depositor

   Marc Bickle, PEPC MPI CBG

   Article

   Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

   Insert

   NcoI-HIS6-3C-NotI-ccdB-AscI-stop-HindIII cassette
   

   Use

   Tags

   HIS6, cleavable with 3C protease

   Expression

   Insect

   Mutation

   Promoter

   polH

   Available since

   Jan. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pPiggyBacATCC LacZ GCTT loxAttP

   Plasmid

   #173496

   Purpose

   piggyBac insect transgenesis destination vector for Golden Gate Cloning assemblies

   Depositor

   Eric Marois

   Article

   Lutrat et al Commun Biol. 2022 Mar 7;5(1):210. doi: 1

   Type

   Empty backbone

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Sept. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. 6X-STAT-synthetic luciferase

    Plasmid

    #37392

    Depositor

    Norbert Perrimon

    Article

    Baeg et al Genes Dev. 2005 Aug 15;19(16):1861-70. E

    Insert

    six STAT consensus sites
    

    Use

    Luciferase

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Hsp70

    Available since

    July 30, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAct-PE2-HygroR

    Plasmid

    #149552

    Purpose

    Expression of PE2 enzyme under control of ubiquitous Actin5c promoter. Can be used to generate stable cell lines by Hygromycin selection.

    Depositor

    Norbert Perrimon

    Article

    Bosch et al Proc Natl Acad Sci U S A. 2021 Jan 5;118

    Insert

    PE2
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Act5c

    Available since

    Aug. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. 20XUAS IVS CsChrimson tdtomato_tr

    Plasmid

    #111544

    Purpose

    Optogenetics in Drosophila: UAS-induced expression of Chrimson tdTomato

    Depositor

    Vivek Jayaraman

    Article

    Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

    Insert

    Chrimson_tdtomato_trafficked
    

    Use

    Tags

    Expression

    Insect

    Mutation

    wt

    Promoter

    hsp70

    Available since

    June 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pIZ-EGFP-MYO10-HMM

    Plasmid

    #87257

    Purpose

    Expression plasmid to perform NanoSPD assays in insect cells (generates EGFP-tagged bait).

    Depositor

    Thomas Friedman

    Article

    Bird et al Mol Biol Cell. 2016 Dec 8. pii: mbc.E16-

    Insert

    MYO10 (Myo10 Mouse)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Myo10 is truncated to contain aa.1-941

    Promoter

    OpIE2

    Available since

    April 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pFastBac His6 MBP N10 TEV LIC cloning vector (4C)

    Plasmid

    #30116

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Use

    Tags

    His6-MBP-N10-TEV

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Aug. 17, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCFD3.1-w-dU6:3gRNA

    Plasmid

    #123366

    Purpose

    gRNA expression plasmid for CRISPR mutagenesis in Drosophila (with mini-white transformation marker).

    Depositor

    Simon Bullock

    Insert

    U6:3-gRNA
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    April 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pUAST-attB-yki:V5-S168A

    Plasmid

    #85617

    Purpose

    Expresses V5 tagged mutant yki in Drosophila.

    Depositor

    Kenneth Irvine

    Article

    Oh et al Oncogene. 2009 Apr 30;28(17):1916-27. do

    Insert

    yki-V5-S168A
    

    Use

    Tags

    V5

    Expression

    Insect

    Mutation

    Promoter

    Available since

    May 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pNDamMyc

    Plasmid

    #59217

    Purpose

    Drosophila DamID vector used to fuse gene of interest to Myc-tagged E.coli Dam.

    Depositor

    Bas van Steensel

    Article

    van Steensel et al Nat Biotechnol. 2000 Apr;18(4):424-8.

    Type

    Empty backbone

    Use

    Damid

    Tags

    Dam and Myc

    Expression

    Insect

    Mutation

    Promoter

    Drosophila heat-shock promoter

    Available since

    May 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pFB1.HMBP.PrS.PHF19

    Plasmid

    #125168

    Purpose

    Expresses human PHF19 in insect cells, , under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tag.

    Depositor

    Chen Davidovich

    Article

    Zhang et al Nat Struct Mol Biol. 2019 Mar;26(3):237-

    Insert

    PHF19 (PHF19 Human)
    

    Use

    Tags

    His-MBP

    Expression

    Insect

    Mutation

    Promoter

    Polyhedrin

    Available since

    April 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pFB1.HMBP.PrS.PHF1

    Plasmid

    #125167

    Purpose

    Expresses human PHF1 in insect cells, , under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tag.

    Depositor

    Chen Davidovich

    Article

    Zhang et al Nat Struct Mol Biol. 2019 Mar;26(3):237-

    Insert

    PHF1 (PHF1 Human)
    

    Use

    Tags

    His-MBP

    Expression

    Insect

    Mutation

    Promoter

    Polyhedrin

    Available since

    April 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. PSL1180polyUBdsRED

    Plasmid

    #49327

    Purpose

    Donor plasmid for homologous recombination that expresses dsRED constitutively using the Aedes aegypti polyubiquitin (PUb) promoter.

    Depositor

    Leslie Vosshall

    Insert

    dsRED
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Aedes polyubiquitin

    Available since

    Nov. 18, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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