4,841 results
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Plasmid#118888Purposeshuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6-MBP tag, cleavable with 3C, and N-terminal SNAP tagDepositorInsertNcoI-HIS6-MBP-3C-SNAP-NotI-ccdB-AscI-stop-HindIII cassette
UseTagsHIS6-MBP, cleavable with 3C protease; SNAP tagExpressionInsectMutationPromoterpolHAvailable sinceJan. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOCC202
Plasmid#118880Purposeshuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with C-terminal mKate2, and C-terminal HIS6 tag, cleavable with 3CDepositorInsertNcoI-NotI-ccdB-AscI-mKate2-3C-HIS6-stop-HindIII cassette
UseTagsmKate2, HIS6, cleavable with 3C proteaseExpressionInsectMutationPromoterpolHAvailable sinceJan. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOCC139
Plasmid#118872Purposeshuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6 tag, cleavable with 3C, and C-terminal monomeric GFPDepositorInsertNcoI-HIS6-3C--NotI-ccdB-AscI-mGFP-stop-HindIII cassette
UseTagsHIS6, cleavable with 3C protease and mGFPExpressionInsectMutationPromoterpolHAvailable sinceJan. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pUC 3GLA
Plasmid#71762Purposegap-repair recombineering for Drosophila transgenesis, contains loxP cassette with GFP, Amp resistantDepositorTypeEmpty backboneUseTagsExpressionInsectMutationPromoterAvailable sinceDec. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSGE_886_Nhe2_EcR-to-ato_RB
Plasmid#71181PurposeLuciferase reporterDepositorInsert886_Nhe2_EcR-to-ato_RB
UseLuciferaseTagsluciferaseExpressionInsectMutationPromoterDSCP Drosophila synthetic core promoterAvailable sinceOct. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
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pFL His-I3 V41A / W43A
Plasmid#113515PurposeExpression of His-I3 V41A / W43 A in insect cells (Baculovirus system)DepositorInsertI3 (PPP1R11 Human)
UseTags6*HisExpressionInsectMutationV41A /W43APromoterAvailable sinceOct. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFL His-I3
Plasmid#113513PurposeExpression of His-I3 in insect cells (Baculovirus system)DepositorInsertI3 (PPP1R11 Human)
UseTags6*HisExpressionInsectMutationPromoterAvailable sinceOct. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pVL-FLAG/His LCOR
Plasmid#114450PurposeInsect baculovirus expression vector containing N terminal FLAG/His tagged human LCOR coding sequenceDepositorInsertLCOR (LCOR Human)
UseTagsFLAG-HisExpressionInsectMutationPromoterAvailable sinceSept. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFastBac-GST-Mps1-KD
Plasmid#114066Purposeprotein productionDepositorInsertMps1-KD (TTK Human)
UseTagsGSTExpressionInsectMutationMps1 KD (D664A)PromoterAvailable sinceSept. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFastBac-GST-Mps1-WT
Plasmid#114065Purposeprotein productionDepositorInsertMps1-WT (TTK Human)
UseTagsGSTExpressionInsectMutationnonePromoterAvailable sinceSept. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pOEM1_pCMV:axFgf8-pPH:vsvged
Plasmid#111159PurposeBaculovirus rescue vector, VSVGED pseudotype, hShhDepositorInsertaxFgf8
UseBaculoviral rescue shuttle vectorTagsExpressionInsect and MammalianMutationwtPromoterCMVAvailable sinceAug. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFastBac-LacI-LAP-HEC1
Plasmid#114033PurposeProduction of baculovirus with LacI-LAP-HEC1DepositorInsertLacI-LAP-HEC1 (NDC80 Human)
UseTagsExpressionInsectMutationnonePromoterCMVAvailable sinceAug. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFastBac Dual PNG172-STII PLU-HIS
Plasmid#112992PurposeBaculovirus expression of Drosophila png172-Flag and plu-HisDepositorUseTagsFlag and HisExpressionInsectMutationpng172 mutationPromoterAvailable sinceAug. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFastBac Dual PNG-STII PLU-HIS
Plasmid#112993PurposeBaculovirus expression of Drosophila png-Flag and plu-HisDepositorUseTagsFlag and HisExpressionInsectMutationPromoterAvailable sinceAug. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pOEM1_pCMV:deltaN-bmp4-pPH:vsvged
Plasmid#111158PurposeBaculovirus rescue vector, VSVGED pseudotype, hShhDepositorInsertdeltaNhBmp4 (BMP4 Human)
UseBaculoviral rescue shuttle vectorTagsExpressionInsect and MammalianMutationdelta N, mutant BMP lacking the N terminus (delta…PromoterCMVAvailable sinceJune 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUAST-attB-Flag-dTSC2 S1338A
Plasmid#111808PurposeInsect Expression of dTSC2DepositorInsertdTSC2 (TSC2 Fly)
UseTags3xFlagExpressionInsectMutationS1338APromoterAvailable sinceJune 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
WNK3 (WNK3B-c005)
Plasmid#110262PurposeBaculovirus expression for structure determination; may not be full ORFDepositorInsertWNK3 (WNK3 Human)
UseTagsHis-TEVExpressionInsectMutationPromoterpolyhedrin promoterAvailable sinceJune 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
WNK3 (WNK3B-c001)
Plasmid#110261PurposeBaculovirus expression for structure determination; may not be full ORFDepositorInsertWNK3 (WNK3 Human)
UseTagsHis-TEVExpressionInsectMutationPromoterpolyhedrin promoterAvailable sinceJune 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
PKN2 (PRKCL2A-c038)
Plasmid#110265PurposeBaculovirus expression for structure determination; may not be full ORFDepositorInsertPRKCL2 (PKN2 Human)
UseTagsHis6-ZBasic-TEVExpressionInsectMutationPromoterpolyhedrin promoterAvailable sinceJune 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
MTHFR (MTHFRA-c042)
Plasmid#110271PurposeBaculovirus expression for structure determination; may not be full ORFDepositorInsertMTHFR (MTHFR Human)
UseTagsTEV-His10-FlagExpressionInsectMutationPromoterpolyhedrin promoterAvailable sinceJune 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
LIMK2 (LIMK2A-c052)
Plasmid#110264PurposeBaculovirus expression for structure determination; may not be full ORFDepositorInsertLIMK2 (LIMK2 Human)
UseTagsHis-TEVExpressionInsectMutationPromoterpolyhedrin promoterAvailable sinceJune 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
Hy_pMT dati MCS Exon
Plasmid#110119PurposeExpression plasmid for expressing circular RNAs of a desired sequence in Drosophila using the dati flanking introns to drive backsplicingDepositorInsertdati (dati Fly)
UseTagsExpressionInsectMutationPromoterMetallothionein Promoter (pMT)Available sinceJune 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
Hy_pMT dati Exons 1-3
Plasmid#110113PurposeDrosophila dati minigene plasmid that expresses both a linear RNA and a circular RNADepositorInsertdati (dati Fly)
UseTagsExpressionInsectMutationPromoterMetallothionein Promoter (pMT)Available sinceJune 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
Hy_pMT dati MCS circfirefly_1100
Plasmid#110117PurposeExpresses a 1100-nt firefly luciferase circular RNA in Drosophila cellsDepositorInsertFirefly luciferase
UseTagsExpressionInsectMutationPromoterMetallothionein Promoter (pMT)Available sinceMay 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
Hy_pMT dati MCS circfirefly_900
Plasmid#110116PurposeExpresses a 900-nt firefly luciferase circular RNA in Drosophila cellsDepositorInsertFirefly luciferase
UseTagsExpressionInsectMutationPromoterMetallothionein Promoter (pMT)Available sinceMay 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
Hy_pMT dati MCS circfirefly_700
Plasmid#110115PurposeExpresses a 700-nt firefly luciferase circular RNA in Drosophila cellsDepositorInsertFirefly luciferase
UseTagsExpressionInsectMutationPromoterMetallothionein Promoter (pMT)Available sinceMay 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
Hy_pMT dati MCS circfirefly_500
Plasmid#110114PurposeExpresses a 500-nt firefly luciferase circular RNA in Drosophila cellsDepositorInsertFirefly luciferase
UseTagsExpressionInsectMutationPromoterMetallothionein Promoter (pMT)Available sinceMay 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGS_N_Strep
Plasmid#101651PurposeExpression of type I signal-peptide containing proteins with N-terminal Twin-Strep-tag in insect cellsDepositorInsertLRP6 Signal peptide, KpnI linker, Twin-Strep tag
UseTagsTwin-Strep tagExpressionInsectMutationPromoterAvailable sinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGS_C_Strep
Plasmid#101652PurposeExpression of type I signal-peptide containing proteins with C-terminal Twin-Strep-tag in insect cellsDepositorInsertLRP6 Signal peptide, MCS linker, Twin-Strep tag
UseTagsTwin-Strep tagExpressionInsectMutationPromoterAvailable sinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only