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pSPL

Plasmid

#110743

Purpose

Plasmid for the insect cell expression system, donor for plasmid fusions, two promoters, Multiplication Module M, LoxP site

Depositor

Tim Richmond

Article

Fitzgerald et al Nat Methods. 2006 Dec;3(12):1021-32. doi

Type

Empty backbone

Expression

Insect

Promoter

Polyhedrin and p10 promoters

Available Since

June 21, 2018

Availability

Academic Institutions and Nonprofits only

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pFB1_hMSH2

Plasmid

#129423

Purpose

Expression human MSH2 protein using baculovirus

Depositor

Peggy Hsieh

Article

Geng et al Anal Biochem. 2011 Jun 15;413(2):179-84.

Insert

human MSH2 (MSH2 Human)

Expression

Insect

Promoter

Polyhedrin promoter

Available Since

Aug. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pFB1_hMSH6

Plasmid

#129424

Purpose

Expression human MSH6 protein using baculovirus

Depositor

Peggy Hsieh

Article

Geng et al Anal Biochem. 2011 Jun 15;413(2):179-84.

Insert

human MSH6 (MSH6 Human)

Expression

Insect

Promoter

Polyhedrin promoter

Available Since

Aug. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pCFD1-dU6:1gRNA

Plasmid

#49408

Purpose

expression of gRNA under control of the Drosophila U6:1 promoter

Depositor

Simon Bullock

Article

Port et al Proc Natl Acad Sci U S A. 2014 Jul 7. pi

Insert

dU6-1:gRNA

Use

CRISPR

Expression

Insect

Promoter

dU6-1

Available Since

Jan. 10, 2014

Availability

Academic Institutions and Nonprofits only

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pJFRC7-syt1-GCaMP6f

Plasmid

#190896

Purpose

GCaMP6f fused to Drosophila synaptotagmin1 via a 3x GS linker. In the pJFRC7-20XUAS vector.

Depositor

Tom Clandinin

Article

Turner et al Elife. 2022 Oct 27;11. pii: 82587. doi:

Insert

syt1-GCaMP6f

Expression

Insect

Available Since

Nov. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pBS-KDRT-STOP-loxP-3XP3::dsRed-loxP-STOP-KDRT-smGdP-10XV5

Plasmid

#217510

Purpose

KD Recombinase dependent conditional smGdP-10XV5 cassette

Depositor

S. Lawrence Zipursky

Article

Sanfilippo et al Neuron. 2024 Jan 13:S0896-6273(23)00973-

Insert

KDRT-STOP-loxP-3XP3::dsRed-loxP-STOP-KDRT-smGdP-10XV5

Tags

smGdP-10XV5

Expression

Insect

Available Since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

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pFastBac-CHD7

Plasmid

#170141

Purpose

Expresses CHD7 in insect cells

Depositor

Robert Kingston

Article

Bouazoune et al Proc Natl Acad Sci U S A. 2012 Nov 20;10

Insert

chromodomain helicase DNA binding protein 7 (CHD7 Human)

Tags

6xHis and FLAG

Expression

Insect

Available Since

July 14, 2021

Availability

Academic Institutions and Nonprofits only

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pTriEx-mVenus-Zdk1

Plasmid

#81047

Purpose

mammalian expression of Zdk1

Depositor

Klaus Hahn

Article

Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

Insert

Zdk1

Tags

6x His tag, FLAG, and mVenus

Expression

Bacterial, Insect, and Mamm…

Available Since

Aug. 16, 2016

Availability

Academic Institutions and Nonprofits only

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pFastBac His6 MBP Asn10 TEV cloning vector with BioBrick PolyPromoter LIC Subcloning (438-C)

Plasmid

#55220

Depositor

Scott Gradia

Article

Gradia et al Methods Enzymol. 2017;592:1-26. doi: 10.

Type

Empty backbone

Tags

His6 and MBP, Asn10

Expression

Insect

Available Since

June 17, 2014

Availability

Academic Institutions and Nonprofits only

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pAV5B+longBRD4

Plasmid

#157792

Purpose

Expression of long isoform of BRD4

Depositor

Michael Rosen

Article

Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

Insert

long BRD4 (BRD4 Human)

Tags

His6

Expression

Insect

Promoter

polyhedron

Available Since

Aug. 5, 2020

Availability

Academic Institutions and Nonprofits only

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pWALIUM-intron 3x UAS attB

Plasmid

#206360

Purpose

includes two standard UAS cassettes and one UAS cassette designed to co-express shRNAs/shRNA clusters and prodein coding sequences

Depositor

Erdem Bangi

Article

Teague et al PLoS Genet. 2023 Jun 2;19(6):e1010792. d

Type

Empty backbone

Expression

Insect

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pBIG1e-CDK9/Cyclin T1

Plasmid

#231732

Purpose

Protein expression in insect cells

Depositor

Jesper Svejstrup

Article

Lopez Martinez et al Mol Cell. 2025 May 15;85(10):1952-1967.e

Inserts

CDK9 (CDK9 Human)
CCNT1 (CCNT1 Human)

Tags

Flag-3C

Expression

Insect

Available Since

May 2, 2025

Availability

Academic Institutions and Nonprofits only

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20XUAS IVS CsChrimson tdtomato_tr

Plasmid

#111544

Purpose

Optogenetics in Drosophila: UAS-induced expression of Chrimson tdTomato

Depositor

Vivek Jayaraman

Article

Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

Insert

Chrimson_tdtomato_trafficked

Expression

Insect

Mutation

wt

Promoter

hsp70

Available Since

June 26, 2018

Availability

Academic Institutions and Nonprofits only

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pC-sleeping beauty

Plasmid

#65487

Purpose

HSP-driven SB100X Sleeping Beauty transposase in pCasper backbone.

Depositor

Guillaume Filion

Insert

sleeping beauty transposase

Expression

Insect

Promoter

HSP

Available Since

Dec. 19, 2017

Availability

Academic Institutions and Nonprofits only

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pAC-14-LEXA-QF

Plasmid

#46104

Depositor

Christopher Potter

Article

Riabinina et al Nat Methods. 2015 Jan 12. doi: 10.1038/n

Insert

LexA-QF

Expression

Insect

Available Since

Aug. 12, 2013

Availability

Academic Institutions and Nonprofits only

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pBig1a zz TEV YBBR RAP1 ZZ TEV TRF2

Plasmid

#185449

Purpose

Coexpresses human RAP1 with an MBP affinity tag, YBBR site, and TEV site, and human TRF2 with a zz tag and TEV site

Depositor

Ahmet Yildiz

Article

Jack et al Dev Cell. 2022 Jan 24;57(2):277-290.e9.

Inserts

TRF2 (TERF2 Human)
RAP1 (TERF2IP Human)

Tags

MBP, YBBR, and ZZ

Expression

Insect

Available Since

June 22, 2022

Availability

Academic Institutions and Nonprofits only

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pBPGAL4.2::VP16Uw

Plasmid

#26228

Depositor

Gerald Rubin

Article

Pfeiffer et al Genetics. 2010 Oct;186(2):735-55. doi: 1

Insert

GAL4::VP16 (GAL4 Budding Yeast)

Expression

Insect

Available Since

Oct. 7, 2010

Availability

Academic Institutions and Nonprofits only

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pBP2MF

Plasmid

#31279

Depositor

Aziz Sancar

Article

Ye et al J Biol Chem. 2011 Jul 22;286(29):25891-9

Insert

Per2 (Per2 Mouse)

Tags

Flag and Myc

Expression

Insect

Available Since

Sept. 22, 2011

Availability

Academic Institutions and Nonprofits only
pACU2

Plasmid

#31223

Depositor

Yuh-Nung Jan

Article

Han et al Proc Natl Acad Sci U S A. 2011 Jun 7;108

Type

Empty backbone

Expression

Insect

Available Since

July 29, 2011

Availability

Academic Institutions and Nonprofits only

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pBS-KS-attB1-2-PT-SA-SD-1-GFP11x7

Plasmid

#172067

Purpose

Protein trap plasmid for inserting GFP11x7 into a phase 1 coding intron

Depositor

Daichi Kamiyama

Article

Kamiyama et al Proc Natl Acad Sci U S A. 2021 Jun 8;118

Insert

GFP11x7

Expression

Insect

Available Since

Oct. 20, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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