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Plasmid#81129Purposemammalian expression of Zdk1 tagged Tiam1 Dh/PHDepositorInsertsUseTagsmCherryExpressionBacterial, Insect, and Mamm…MutationPromoterAvailable sinceAug. 30, 2016AvailabilityAcademic Institutions and Nonprofits only
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pKDM
Plasmid#110740PurposePlasmid for the insect cell expression system, two promoters, Multiplication Module M, Tn7 transpositionDepositorTypeEmpty backboneUseTagsExpressionInsectMutationPromoterAvailable sinceJune 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBS-KS-attB1-2-PT-SA-SD-1-GFP11x7
Plasmid#172067PurposeProtein trap plasmid for inserting GFP11x7 into a phase 1 coding intronDepositorInsertGFP11x7
UseTagsExpressionInsectMutationPromoterAvailable sinceOct. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCFD3.1-w-dU6:3gRNA
Plasmid#123366PurposegRNA expression plasmid for CRISPR mutagenesis in Drosophila (with mini-white transformation marker).DepositorInsertU6:3-gRNA
UseCRISPRTagsExpressionInsectMutationPromoterAvailable sinceApril 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBS-KS-attB1-2-PT-SA-SD-1-GFP11
Plasmid#172068PurposeProtein trap plasmid for inserting GFP11 into a phase 1 coding intronDepositorInsertGFP11x7
UseTagsExpressionInsectMutationPromoterAvailable sinceOct. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFastBac1-His10FLAG
Plasmid#157736Purposeempty pFastBac1 vector (polyhedrin promoter) adds enterokinase-removable N-terminal His10-tag + 3xFLAG tag; avoids unwanted residues in the final protein sequence by using the BseRI restriction sitesDepositorTypeEmpty backboneUseTagsHis10 + 3xFLAG, enterokinase-cleavableExpressionInsectMutationPromoterpolyhedrinAvailable sinceJune 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFast-Flag-hRad9
Plasmid#222468PurposeExpresses hRad9 in insect cellsDepositorInserthRad9 (RAD9A Human)
UseTagsFlag epitopeExpressionInsectMutationPromoterAvailable sinceDec. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFast-hRad1
Plasmid#222471PurposeExpresses hRad1 in insect cellsDepositorInserthRad1 (RAD1 Human)
UseTagsExpressionInsectMutationPromoterAvailable sinceDec. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFast-hHus1
Plasmid#222469PurposeExpresses hHus1 in insect cellsDepositorInserthHus1 (HUS1 Human)
UseTagsExpressionInsectMutationPromoterAvailable sinceDec. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFast-Flag-hHus1
Plasmid#222470PurposeExpresses hHus1 in insect cellsDepositorInserthHus1 (HUS1 Human)
UseTagsFlag epitopeExpressionInsectMutationPromoterAvailable sinceDec. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFast-His6-hRad17
Plasmid#222465PurposeExpresses hRad17 in insect cellsDepositorInserthRad17 (RAD17 Human)
UseTagsHisExpressionInsectMutationPromoterAvailable sinceDec. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
20XUAS IVS CsChrimson tdtomato_tr
Plasmid#111544PurposeOptogenetics in Drosophila: UAS-induced expression of Chrimson tdTomatoDepositorInsertChrimson_tdtomato_trafficked
UseTagsExpressionInsectMutationwtPromoterhsp70Available sinceJune 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEGFP
Plasmid#165830PurposeEGFP green fluorescent protein CDSDepositorInsertEGFP
UseTagsExpressionInsectMutationPromoterAvailable sinceSept. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pACUH-GFP11x7-mCherry-α-tubulin
Plasmid#70218PurposeExpresses GFP11x7-mCherry-α-tubulin in insect cells. 5 a. a. linker lengths.DepositorInsertGFP11x7-mCherry-α-tubulin
UseTagsGFP11x7 and mCherryExpressionInsectMutationPromoterAvailable sinceMarch 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBPGUw-HACK-G4>QF2
Plasmid#80277PurposeGAL4->QF2 HACK construct compatible with GMR attP integrated insertions. Contains 5' and 3' GAL4 homology arms and gRNAs.DepositorInsertQF2
UseTagsExpressionInsectMutationPromoterAvailable sinceJuly 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJFRC19-13XLexAop2-IVS-myr::GFP
Plasmid#26224DepositorInsertmyr::GFP
UseTagsExpressionInsectMutationPromoterAvailable sinceOct. 19, 2010AvailabilityAcademic Institutions and Nonprofits only -
pFAST-BAC BAF155/SMARCC1-His
Plasmid#177863PurposeTransfer vector to generate recombinant baculovirus expressing BAF155/SMARCC1-HisDepositorInsertSMARCC1 (SMARCC1 Human)
UseTagsHis and His tag at C-terminus with GGGGS linkerExpressionInsectMutationPromoterpolyhedrinAvailable sinceDec. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHACK-GAL4>QF2(newHA1)
Plasmid#104873PurposeHACK Donor plasmids for converting GAL4 lines to QF2 lines using HACK method. Updated version of Addgene#80275 for easier cloning of insertsDepositorInsertT2A-QF2
UseCRISPRTagsExpressionInsectMutationPromoterAvailable sinceMarch 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTriEx-mCherry-LOV2
Plasmid#81041PurposeMammalian or insect expression for wt LOV2DepositorInsertLOV2
UseTagsmCherryExpressionBacterial, Insect, and Mamm…MutationPromoterCMV, p10Available sinceAug. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
pE5n
Plasmid#17459Purposemodified pENTR vector for N-terminal ECFP tag fusion with your gene of interestDepositorTypeEmpty backboneUseModified gateway entry vectorTagsECFPExpressionBacterial, Insect, Mammalia…MutationPromoterAvailable sinceMarch 21, 2008AvailabilityAcademic Institutions and Nonprofits only