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Plasmid#111657PurposeC-terminal GFP tag on BICDR1 for expression in Sf9 cells. 8xHis-ZZ-GFP N-terminal tag, TEV site to cleave 8xHis-ZZ.DepositorInsertBICDR1-GFP (Bicdl1 Mouse)
UseTags8xHis-tag, GFP, TEV site, and ZZExpressionInsectMutationOptimised for Sf9 expressionPromoterAvailable sinceJuly 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
OA-1050J (GFP)
Plasmid#133304Purposeexpress arrays of gRNA targeting GFP under dU6-3 promoterDepositorInsertGFP gRNA array
UseTagsExpressionInsectMutationPromoterU6-3Available sinceSept. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTriEx-mCherry-LINXa3-nano
Plasmid#73616PurposeLight-induced nuclear export switch fusion to nano for microscopy in mammalian cells.DepositorInsertLINXa3-nano
UseTagsmCherryExpressionBacterial, Insect, and Mamm…MutationPromoterAvailable sinceApril 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
dBrainbow
Plasmid#29327DepositorInsertUAS-Stop-GFP:HSV-mTFP1:HA-mKO2:V5
UseTagsExpressionInsectMutationPromoterAvailable sinceNov. 9, 2011AvailabilityAcademic Institutions and Nonprofits only -
pIE-hα2M-H6
Plasmid#128492PurposeExpresses human α2M (from S24 to N1473) in insect cells. With C-t H6x tag.DepositorInsertA2M (A2M Human)
UseTagsHis tag (6x)ExpressionInsectMutationPromoterIE1Available sinceAug. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
52 pLIB_3C_C_STREP
Plasmid#160300PurposeC-term 3C cleavable 2xStrepII tag for insect cell expressionDepositorTypeEmpty backboneUseTags2xStrepIIExpressionInsectMutationPromoterpolyhydrinAvailable sinceNov. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pActPL-VP16AD
Plasmid#15305DepositorInsertVP16AD-ZIP+
UseTagsExpressionInsectMutationPromoterAvailable sinceJuly 6, 2007AvailabilityAcademic Institutions and Nonprofits only -
pMT Stat92E-GFP
Plasmid#126662PurposeInducible expression of Stat92E-GFP in S2 cellsDepositorInsertStat29E
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
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CDK12
Plasmid#73258PurposeBaculovirus expression for structure determination; may not be full ORFDepositorInsertCDK12 (CDK12 Human)
UseTagsHis-6-TEVExpressionInsectMutationpfam00069: Pkinase 727-1020PromoterPolyhedrinAvailable sinceApril 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGE-attB-GMR_mNeonGreen::3XFLAG::dCrk
Plasmid#131143PurposeRescue construct for targeting into crk[ΔattP]. Contains 110bp upstream of transcription start site, mNeonGreen, 3XFLAG, and dCrk cDNA, 3' UTR and 99bp downstream.DepositorInsertmNeonGreen::3XFLAG::Crk (Crk Fly)
UseCre/Lox; Attb recombination siteTags3XFLAG and mNeonGreenExpressionInsectMutationPromoterAvailable sinceOct. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pR I-Ab alpha BirA
Plasmid#113638PurposeInsect cell expression vector for I-Ab alpha chain fused to BirA biotinylation siteDepositorInsertI-Ab alpha BirA (H2-Ab1 Mouse)
UseTagsBirA biotinylation signal site and fused to Fos-J…ExpressionInsectMutationtransmembrane and cytoplasmic domains truncatedPromoterMetallothioneinAvailable sinceSept. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
GST-3C-FIP200-GFP
Plasmid#187832PurposePlasmid for the expression and purification of GST-3C-FIP200-GFP. Internal reference: SMC1445DepositorInsertFIP200 (RB1CC1 Human)
UseTagsGST and mGFPExpressionBacterial and InsectMutationPromoterAvailable sinceOct. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCRISPaint-T2A-Cas9-T2A-GFP-3xP3-RFP
Plasmid#127560PurposeCRISPaint universal donor plasmid for gene exon insertion. Encodes T2A-Cas9-T2A-GFP-SV40 and 3xP3-RFP visible eye marker.DepositorInsertCas9-T2A-GFP
UseCRISPRTagsExpressionInsectMutationEGFP-D102G see depositor comments belowPromoterAvailable sinceJuly 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBac-DsRed-ORCO_9kbProm-QF2
Plasmid#104877PurposeAnopheles gambiae ORCO enhancer/promter to drive QF2 expression in Anopheles transgenic mosquitoesDepositorInsertAgORCO-QF2
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBac-GH146-QF
Plasmid#24353DepositorInsertQF
UseTagsdsRedExpressionInsectMutationPromoterAvailable sinceApril 12, 2010AvailabilityAcademic Institutions and Nonprofits only -
pFastBac HTB DPP9S S730A
Plasmid#169946PurposePurification of catalytically-dead DPP9S from insect cellsDepositorInsertDPP9 (DPP9 Human)
UseTagsHIS-TEVExpressionInsectMutationS730APromoterpolyhedrinAvailable sinceMay 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
Ac5-STABLE1-puro
Plasmid#208031PurposeEnables expression of an ORF (with or without tags) and selection with puromycin in insect cells. Includes GFP-T2A-puro with Actin5C promoter.DepositorTypeEmpty backboneUseTagsExpressionInsectMutationPromoterAvailable sinceOct. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
DPYSL4 (DPYSL4A-c003)
Plasmid#110263PurposeBaculovirus expression for structure determination; may not be full ORFDepositorInsertDPYSL4 (DPYSL4 Human)
UseTagsHis6-ZBasic-TEVExpressionInsectMutationPromoterpolyhedrin promoterAvailable sinceJune 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTSTEP
Plasmid#72334PurposeTissue-specifically swappable (C-terminal TagRFPT-to-EGP) genome tagging plasmid with Golic+ selection cassette for fully transgenic gene targeting (Drosophila melanogaster)DepositorInsertRRS-TagRFPT-p10-[Golic+] RRS-EGFP
UseCRISPRTagsExpressionInsectMutationPromoterAvailable sinceJan. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDam-Myc-HP1
Plasmid#59221Purposeexpresses Dam-myc-HP1DepositorInsertHP1 (Su(var)205 Fly)
UseDamidTagsDam and mycExpressionInsectMutationPromoterDrosophila heat shock promoterAvailable sinceMay 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
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pFastBac_Dual_GST-TEV-EGFP-TBK1
Plasmid#187830PurposePlasmid for the expression and purification of GST-TEV-EGFP-TBK1 in Spodoptera frugiperda cells (Sf9). Internal reference: SMC1631DepositorInsertTBK1 (TBK1 Human)
UseTagsGST and TEV-mEGFP-GG LinkerExpressionBacterial and InsectMutationPromoterAvailable sinceOct. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
CapChR2_pUAST-attb
Plasmid#188041PurposeExpresses CapChR2 in DrosophilaDepositorInsertCapChR2
UseTagsnoneExpressionInsectMutationS43D, L112C, T139C and N238E mutations in CoChRPromoterUASAvailable sinceDec. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pQUAST-shibire (ts1)
Plasmid#24362DepositorInsertShibire (ts1) (shi Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceMay 10, 2010AvailabilityAcademic Institutions and Nonprofits only -
pattB-nsyb-M13::GAL4DBDo
Plasmid#64720Purposegenerating transgenic TRIC fliesDepositorInsertM13::GAL4DBDo
UseTagsExpressionInsectMutationPromoterAvailable sinceMay 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTriEx4-H6-FGB
Plasmid#49203PurposeFluorescent reporter for cGMPDepositorInsertH6-FGB
UseTagsHis tag + Other tagsExpressionBacterial, Insect, and Mamm…MutationNonePromoterCMVAvailable sinceDec. 4, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCFD3-Act5c
Plasmid#130278PurposeDrosophila expression of sgRNA that targets Actin5c CDS close to stop codon. Positive control sgRNA for CRISPaint knock-ins in cultured cells.DepositorInsertpCFD3-Act5c (Act5C Fly)
UseCRISPRTagsExpressionInsectMutationPromoterAvailable sinceAug. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pUASt-GBP-Trbo
Plasmid#179996PurposeExpresses two tandem copies of the GFP nanobody fused at the C-terminus with TurboID. Expression is driven using the UAS-Gal4 system. The UASt version is ideal for expression in somatic tissues.DepositorInsertGBP nanobody
UseTagsTrboExpressionInsectMutationPromoterAvailable sinceMarch 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUAS-kkv::NG
Plasmid#138953PurposeUsed to generate a transgene that will express a fluorescent Chitin Synthase in Drosophila using Gal4DepositorArticleInsertkkv (NEWENTRY Fly)
UseUas transgene vectorTagsNeon Green fused to C terminus of KkvExpressionInsectMutationPromoterAvailable sinceNov. 1, 2020AvailabilityAcademic Institutions and Nonprofits only