4,932 results

1. pFastBac-CHD7 (787-1899)

   Plasmid

   #170145

   Purpose

   Expresses residues 787-1899 of CHD7 in insect cells

   Depositor

   Robert Kingston

   Article

   Bouazoune et al Proc Natl Acad Sci U S A. 2012 Nov 20;10

   Insert

   chromodomain helicase DNA binding protein 7 (CHD7 Human)
   

   Tags

   FLAG

   Expression

   Insect

   Mutation

   N-terminal truncation of residues 1-786; C-termin…

   Available Since

   July 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pFastBac-CHD7 (787-2138)

   Plasmid

   #170146

   Purpose

   Expresses residues 787-2138 of CHD7 in insect cells

   Depositor

   Robert Kingston

   Article

   Bouazoune et al Proc Natl Acad Sci U S A. 2012 Nov 20;10

   Insert

   chromodomain helicase DNA binding protein 7 (CHD7 Human)
   

   Tags

   FLAG

   Expression

   Insect

   Mutation

   N-terminal truncation of residues 1-786; C-termin…

   Available Since

   July 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pFastBac-CHD7 (1-1547)

   Plasmid

   #170144

   Purpose

   Expresses residues 1-1547 of CHD7 in insect cells

   Depositor

   Robert Kingston

   Article

   Bouazoune et al Proc Natl Acad Sci U S A. 2012 Nov 20;10

   Insert

   chromodomain helicase DNA binding protein 7 (CHD7 Human)
   

   Tags

   FLAG

   Expression

   Insect

   Mutation

   C-terminal truncation of residues 1548-2997

   Available Since

   July 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pFastBac-CHD7 (936-2997)

   Plasmid

   #170147

   Purpose

   Expresses residues 936-2997 of CHD7 in insect cells

   Depositor

   Robert Kingston

   Article

   Bouazoune et al Proc Natl Acad Sci U S A. 2012 Nov 20;10

   Insert

   chromodomain helicase DNA binding protein 7 (CHD7 Human)
   

   Tags

   FLAG

   Expression

   Insect

   Mutation

   N-terminal truncation of residues 1-935

   Available Since

   July 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pFastBac-CHD7 (K999R)

   Plasmid

   #170142

   Purpose

   Expresses CHD7 (K999R) in insect cells

   Depositor

   Robert Kingston

   Article

   Bouazoune et al Proc Natl Acad Sci U S A. 2012 Nov 20;10

   Insert

   chromodomain helicase DNA binding protein 7 (CHD7 Human)
   

   Tags

   6xHis and FLAG

   Expression

   Insect

   Mutation

   K999R

   Available Since

   July 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. EKAR2G_design1_mTFP_227_Venus_173

   Plasmid

   #39835

   Depositor

   Olivier Pertz

   Article

   Fritz et al Sci Signal. 2013 Jul 23;6(285):rs12. doi

   Insert

   Erk activity reporter
   

   Tags

   His-Myc

   Expression

   Bacterial, Insect, and Mamm…

   Promoter

   CMV

   Available Since

   July 5, 2013

   Availability

   Academic Institutions and Nonprofits only

7. pBPHLWL

   Plasmid

   #26254

   Depositor

   Tom Clandinin

   Article

   Gohl et al Nat Methods. 2011 Mar;8(3):231-7. doi: 1

   Insert

   Multiple cloning site
   

   Use

   Drosophila transgenesis

   Expression

   Bacterial and Insect

   Available Since

   Feb. 2, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pFastBac K-M33

   Plasmid

   #1986

   Depositor

   Robert Kingston

   Article

   Lavigne et al Mol Cell 2004 Feb 13;13(3):415-25.

   Insert

   M33 (Cbx2 Mouse)
   

   Expression

   Insect

   Mutation

   Kozak sequence added before ATG

   Available Since

   March 23, 2006

   Availability

   Academic Institutions and Nonprofits only

9. 57 pLIB_C_CBP

   Plasmid

   #160306

   Purpose

   C-term uncleavable CBP tag for insect cell expression

   Depositor

   John Weir

   Article

   Altmannova et al Protein Sci. 2020 Sep 21. doi: 10.1002/p

   Type

   Empty backbone

   Expression

   Insect

   Promoter

   polyhydrin

   Available Since

   Dec. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pBacPAK8-His-myc-Rbx1

    Plasmid

    #29506

    Depositor

    Joan Conaway, Ronald Conaway

    Article

    Kamura et al Proc Natl Acad Sci U S A. 2000 Sep 12. 9

    Insert

    Rbx1 (Rbx1 Mouse)
    

    Tags

    His and myc

    Expression

    Insect

    Available Since

    April 27, 2011

    Availability

    Academic Institutions and Nonprofits only

11. pGL3-DSCP-Gateway

    Plasmid

    #71507

    Purpose

    flySTARR-seq validation vector with Gateway cassette downstream of the firefly luciferase gene and the DSCP core promoter

    Depositor

    Alexander Stark

    Article

    Zabidi et al Nature. 2015 Feb 26;518(7540):556-9. doi

    Type

    Empty backbone

    Use

    Flystarr-seq validation vector

    Tags

    luciferase

    Expression

    Insect

    Available Since

    Dec. 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. p2Bac.Flag.p34

    Plasmid

    #39221

    Depositor

    Aziz Sancar

    Insert

    p34 (GTF2H3 Human)
    

    Tags

    Flag

    Expression

    Insect

    Promoter

    Pph

    Available Since

    Aug. 24, 2012

    Availability

    Academic Institutions and Nonprofits only

13. pAC-U63-tgRNA-nlsBFP

    Plasmid

    #169029

    Purpose

    gRNA-marker vector contain a U6:3 promoter, gRNA scaffold, and a Ubi-mTagBFP-NLS marker.

    Depositor

    Chun Han

    Article

    Allen et al PLoS Biol. 2021 Jan 14;19(1):e3001061. d

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Insect

    Promoter

    U6:3

    Available Since

    June 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. SPRR2A-pFastBac I

    Plasmid

    #182460

    Purpose

    Expression His-tagged SPRR2A in insect cells

    Depositor

    Lora Hooper

    Article

    Hu et al Science. 2021 Nov 5;374(6568):eabe6723.

    Insert

    small proline rich protein 2A (SPRR2A Human)
    

    Tags

    6xHis

    Expression

    Insect

    Promoter

    Polyhedrin

    Available Since

    March 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pIS1

    Plasmid

    #74887

    Purpose

    targeting vector for C-terminal eGFP tag on Drosophila melanogaster Act5C

    Depositor

    Klaus Foerstemann

    Article

    Kunzelmann et al G3 (Bethesda). 2016 Jun 1;6(6):1777-85.

    Inserts

    Act5C upstream homology region
    Act5C downstream homology
    

    Tags

    eGFP

    Expression

    Insect

    Available Since

    April 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pExpreS2-1-CR-PA-10H-SIII

    Plasmid

    #175447

    Purpose

    HiFi-compatible destination vector for secreted overexpression of thrombin-cleavable, C-terminal Protein A-10His-TwinStrep tagged proteins with ExpreS2 Platform

    Depositor

    Wyatt Yue

    Article

    Coker et al Front Bioeng Biotechnol. 2022 May 5;10:8

    Type

    Empty backbone

    Tags

    BiP Secretion Peptide and Thrombin-Protein A-10Hi…

    Expression

    Insect

    Promoter

    Fused Actin-HSP70

    Available Since

    March 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pJFRC176-10XUAS-rox>-dSTOP-rox>-myr::GFP

    Plasmid

    #32147

    Depositor

    Gerald Rubin

    Article

    Nern et al Proc Natl Acad Sci U S A. 2011 Aug 9.

    Insert

    rox> STOP STOP rox>
    

    Expression

    Insect

    Available Since

    Sept. 23, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pFastBac FPC

    Plasmid

    #1927

    Depositor

    Robert Kingston

    Article

    Francis et al Mol Cell 2001 Sep;8(3):545-56.

    Insert

    Polycomb (Pc Fly)
    

    Tags

    flag

    Expression

    Insect

    Available Since

    March 23, 2006

    Availability

    Academic Institutions and Nonprofits only

19. pKM463

    Plasmid

    #134214

    Purpose

    Baculovirus expression of CENP-U and CENP-Q

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Mol Cell. 2015 Dec 17;60(6):886-98. doi:

    Insert

    his-CENP-U + Q
    

    Expression

    Insect

    Available Since

    Feb. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLib6.4B

    Plasmid

    #176665

    Purpose

    Expression of sgRNA under D. melanogaster U6-2 _(_CR32867) promoter & delivery of D.mel-Actin5C::EBFP2-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism

    Depositor

    Norbert Perrimon

    Article

    Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

    Type

    Empty backbone

    Use

    Crispr

    Expression

    Insect

    Available Since

    Nov. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. Nup98-pDONR221

    Plasmid

    #124241

    Purpose

    Gateway entry clone of Drosophila Nup98-96 cDNA.

    Depositor

    Peter Vilmos

    Article

    Kristo et al Biochim Biophys Acta Mol Cell Res. 2017

    Insert

    Drosophila Nup98-96 (Nup98-96 Fly)
    

    Expression

    Insect

    Available Since

    April 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. HS-H3.3A-GFP

    Plasmid

    #8696

    Depositor

    Kami Ahmad

    Article

    Ahmad et al Mol Cell 2002 Jun;9(6):1191-200.

    Insert

    H3.3A (His3.3A Fly)
    

    Tags

    GFP

    Expression

    Insect

    Available Since

    Aug. 15, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pJC-TALE-VP64

    Plasmid

    #46147

    Depositor

    David Stern

    Article

    Crocker et al Nat Methods. 2013 Aug;10(8):762-7. doi:

    Type

    Empty backbone

    Tags

    VP64 activation domain

    Expression

    Insect

    Promoter

    hsp70

    Available Since

    Aug. 2, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. ninaE-dsocs

    Plasmid

    #26857

    Depositor

    Craig Montell

    Article

    Wang et al Neuron. 2008 Jan 10. 57(1):56-68.

    Insert

    Slow Termination of Phototransduction (stops Fly)
    

    Tags

    myc

    Expression

    Insect

    Mutation

    This STOPS construct contains a mutated/truncated…

    Available Since

    April 5, 2011

    Availability

    Academic Institutions and Nonprofits only

25. pKM498

    Plasmid

    #134215

    Purpose

    Baculovirus expression of CENP-T and CENP-W

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Mol Cell. 2015 Dec 17;60(6):886-98. doi:

    Insert

    his-CENPT + CENPW
    

    Expression

    Insect

    Available Since

    Feb. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pXN-attPGal4LWL

    Plasmid

    #26261

    Depositor

    Tom Clandinin

    Article

    Gohl et al Nat Methods. 2011 Mar;8(3):231-7. doi: 1

    Insert

    GAL4 (GAL4 Budding Yeast)
    

    Use

    Drosophila transgenesis

    Expression

    Bacterial and Insect

    Available Since

    Feb. 2, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. 10XUAS IVS Jaws mVenus_tr

    Plasmid

    #111553

    Purpose

    Optogenetics in Drosophila: UAS-induced expression of Jaws mVenus

    Depositor

    Vivek Jayaraman

    Article

    Chuong et al Nat Neurosci. 2014 Aug;17(8):1123-9. doi

    Insert

    Jaws_mVenus_tr
    

    Expression

    Insect

    Mutation

    wt

    Promoter

    hsp70

    Available Since

    June 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pAc5.1B-lambdaN-HA-DmFMR1_B

    Plasmid

    #145940

    Purpose

    Insect Expression of DmFMR1

    Depositor

    Elisa Izaurralde

    Insert

    DmFMR1 (Fmr1 Fly)
    

    Expression

    Insect

    Mutation

    Two amino acid mutations (N45D and G558R) and thr…

    Available Since

    Feb. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pUAST mito roGFP2-Orp1

    Plasmid

    #64997

    Purpose

    for expression of mitochondrial roGFP2-Orp1 with the Gal4 expression system in drosophila

    Depositor

    Tobias Dick

    Article

    Albrecht et al Cell Metab. 2011 Dec 7;14(6):819-29. doi

    Insert

    Orp1 (HYR1 Budding Yeast)
    

    Tags

    roGFP2

    Expression

    Insect

    Mutation

    mitochondrial targeting sequence

    Available Since

    June 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pMT p85-GFP

    Plasmid

    #126660

    Purpose

    Inducible expression of p85-GFP in S2 cells

    Depositor

    Ron Vale

    Article

    Williamson et al J Cell Biol. 2018 Nov 5;217(11):3977-399

    Insert

    PI3K SH2 containing subunit
    

    Expression

    Insect

    Available Since

    May 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pUAST tdTomato Rab11 WT

    Plasmid

    #53473

    Depositor

    Matthew Scott

    Insert

    Rab11 (Rab11 Fly)
    

    Tags

    tdtomato

    Expression

    Insect

    Promoter

    GAL4

    Available Since

    June 4, 2014

    Availability

    Academic Institutions and Nonprofits only

32. pPTluc

    Plasmid

    #87789

    Purpose

    Luciferase reporter for testing enhancer fragments in Drosophila

    Depositor

    Herman Wijnen

    Article

    Sharp et al Nucleic Acids Res. 2017 Apr 12. doi: 10.

    Type

    Empty backbone

    Tags

    luciferase

    Expression

    Insect

    Promoter

    minimal promoter

    Available Since

    April 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pOCC93

    Plasmid

    #118911

    Purpose

    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with GP64 viral secretion signal and C-terminal eGFP-HIS6

    Depositor

    PEPC MPI CBG, Marc Bickle

    Article

    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

    Insert

    NcoI-GP64ss-NotI-ccdB-AscI-eGFP-HIS6-stop-HindIII cassette
    

    Tags

    eGFP-HIS6

    Expression

    Insect

    Promoter

    polH

    Available Since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pAcGP67B MBP-tagged human Norrin ( 31–133)

    Plasmid

    #132625

    Purpose

    expresses MBP-tagged human Norrin ( 31–133) in Hi5 insect cell

    Depositor

    Wenqing Xu

    Article

    Shen et al Cell Res. 2015 Sep;25(9):1078-81. doi: 1

    Insert

    MBP-Norrin ( 31–133)
    

    Expression

    Insect

    Available Since

    Oct. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. AeU6 3p3 GFP LgRNA

    Plasmid

    #182208

    Purpose

    For generation of plasmids for CRISPR-mediated 3p3 GFP knockin in Aedes aegypti

    Depositor

    Craig Montell

    Article

    Chen et al Genetics. 2021 Nov 5;219(3). pii: 636806

    Insert

    3xP3-GFP
    

    Use

    CRISPR

    Expression

    Insect

    Promoter

    U6

    Available Since

    April 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pAct-dCBEevoAPOBEC1

    Plasmid

    #195514

    Purpose

    Ubiquitous expression of dCBEevoAPOBEC1 in Drosophila

    Depositor

    Michael Boutros

    Article

    Doll et al Sci Adv. 2023 Sep;9(35):eadj1568. doi: 1

    Insert

    dCBE-evoAPOBEC1
    

    Use

    CRISPR

    Expression

    Insect

    Promoter

    act5C

    Available Since

    Sept. 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pFTSG

    Plasmid

    #162389

    Purpose

    Expression of proteins with a C-terminal thermostable GFP (TGP) tag in insect cell

    Depositor

    Dianfan Li

    Article

    Cai et al Commun Biol. 2020 Dec 10;3(1):753. doi:

    Insert

    thermostable GFP (TGP)
    

    Tags

    His

    Expression

    Insect

    Available Since

    March 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pFastBac HT JS-Munc18b

    Plasmid

    #135554

    Purpose

    Express rat Munc18b in Sf9 cells, the resulted plasmid encodes an N-terminally His6-tagged Munc18c protein with a tobacco etch virus (TEV) cleavage site between the His6 tag and Munc18b.

    Depositor

    Jingshi Shen

    Article

    Yu et al Methods Mol Biol. 2019;1860:237-249. doi

    Insert

    Munc18b (Stxbp2 Rat)
    

    Tags

    6xHis tag and TEV site

    Expression

    Insect

    Promoter

    polyhedrin promoter

    Available Since

    March 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pUASTLOTattB_mCherry::Nrv1::vhhGFP4

    Plasmid

    #163926

    Purpose

    Expression of GrabFP-B-intracellular under control of UAS or LOP enhancer

    Depositor

    Markus Affolter

    Article

    Harmansa et al Elife. 2017 Apr 11;6. doi: 10.7554/eLife

    Insert

    Fusion of vhhGPF4 (intracellular) nanobody with Nrv1 protein and mCherry fluorophor
    

    Use

    Cre/Lox

    Tags

    mCherry

    Expression

    Insect

    Available Since

    Jan. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. 907L

    Plasmid

    #160237

    Purpose

    FlightN-ttgR-VP16-Sv40-attB

    Depositor

    Omar Akbari

    Article

    Gamez et al Insect Mol Biol. 2021 Oct;30(5):461-471.

    Inserts

    TtgR repressor
    Flightin promoter
    

    Tags

    VP16

    Expression

    Insect

    Available Since

    Nov. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge