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5,038 results

Showing: 1801 - 1850 of 5038 results

  6. pOCC81

    Plasmid
    #118900
    Purpose
    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6, cleavable with 3C , and N-terminal GST
    Depositor
    Marc Bickle, PEPC MPI CBG
    Article
    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do
    Insert
    NcoI-HIS6-3C-GST-NotI-ccdB-AscI-stop-HindIII cassette
    Tags
    HIS6, cleavable with 3C protease; GST tag
    Expression
    Insect
    Promoter
    polH
    Available Since
    Jan. 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    118900-plasmid-map-sequence-id-232307

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  7. LHP1049 - pFB-YCK2

    Plasmid
    #32433
    Depositor
    Linda Hicke
    Insert
    YCK2 (YCK2 Budding Yeast)
    Expression
    Insect
    Mutation
    Y193L
    Promoter
    P-P10 and P-Pol
    Available Since
    Nov. 16, 2011
    Availability
    Academic Institutions and Nonprofits only

  11. Abr in pAcG2T

    Plasmid
    #38162
    Depositor
    Nora Heisterkamp
    Insert
    ABR (ABR Human)
    Tags
    GST
    Expression
    Insect
    Promoter
    polyhedron
    Available Since
    Aug. 27, 2012
    Availability
    Academic Institutions and Nonprofits only

  23. Or45b-Gal4

    Plasmid
    #63036
    Purpose
    To express Gal4 in the pattern of the Drosophila melanogaster odorant receptor Or45b, using the promoter sequences of the gene cloned 5' of Gal4
    Depositor
    Leslie Vosshall
    Article
    Fishilevich et al Curr Biol. 2005 Dec 6;15(23):2086-96.
    Insert
    Drosophila melanogaster Or45b promoter
    Tags
    Drosophila ADH (aa's 1-18) fused to Yeast Ga…
    Expression
    Insect
    Available Since
    April 22, 2015
    Availability
    Academic Institutions and Nonprofits only

  25. pGP-20XUAS-IVS-Syn21-op1-jGCaMP7s-T2A-tdTomato-p10

    Plasmid
    #172916
    Purpose
    Gal4 activated transgene coexpression of protein calcium sensor and tdTomato under the hsp70 promoter in D. melanogaster
    Depositor
    GENIE Project
    Insert
    jGCaMP7s-T2A-tdTomato
    Tags
    6xHis and T2A-tdTomato
    Expression
    Insect
    Promoter
    hsp70
    Available Since
    Aug. 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    172916-plasmid-map-sequence-id-341478

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  30. pBac[3xP3-gTc'v; Tc'aTub1P-loxP(mCherry)Sv40_Tc'H2Av_EGFP]

    Plasmid
    #86456
    Purpose
    Lox responder construct with floxed (same orientation; 2xloxP, 2xlox2272) mCherry (cy) body marker and H2Av::EGFP (nu) for Cre dependent cell clone marking; (Transformation plasmid; Black eye marker)
    Depositor
    Gregor Bucher
    Insert
    3xP3-gTc'v; Tc'aTub1P-loxP(mCherry)Sv40_Tc'H2Av_EGFP
    Expression
    Insect
    Available Since
    March 22, 2017
    Availability
    Academic Institutions and Nonprofits only
    86456-plasmid-map-sequence-id-171156

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  34. pLIB_3xFlag-His-PLpro (nsp3) (SARS-CoV-2)

    Plasmid
    #169194
    Purpose
    Baculoviral transfer vector to express SARS-CoV-2 papain-like protease domain (nsp3) in insect cells
    Depositor
    John Diffley
    Article
    Lim et al Biochem J. 2021 Jul 16;478(13):2517-2531
    Insert
    3xFlag-His6-PLpro (ORF1ab SARS-CoV-2, Synthetic)
    Tags
    3xFlag-His6
    Expression
    Insect
    Mutation
    Codon optimised for insect cells (Spodoptera frug…
    Promoter
    Polyhedrin
    Available Since
    July 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    169194-plasmid-map-sequence-id-333980

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  45. pBIG1b_nsp7-Linker-nsp8 (SARS-CoV-2)

    Plasmid
    #169181
    Purpose
    Baculoviral transfer vector to express untagged nsp7-Linker-nsp8 fusion protein (SARS-CoV-2) in insect cells
    Depositor
    John Diffley
    Article
    Bertolin et al Biochem J. 2021 Jul 16;478(13):2425-2443
    Insert
    nsp7-Linker-nsp8 (ORF1ab SARS-CoV-2, Synthetic)
    Tags
    No purification tag and nsp7-nsp8 fusion protein …
    Expression
    Insect
    Mutation
    Codon optimised for insect cells (Spodoptera frug…
    Promoter
    Polyhedrin
    Available Since
    July 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    169181-plasmid-map-sequence-id-334012

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  50. pSBL_DK198

    Plasmid
    #156425
    Purpose
    Insect cell expression of 3a delta N(aa 42-275)-sfGFP, needs to be introduced into baculoviral bacmid
    Depositor
    Stephen Brohawn
    Article
    Kern et al bioRxiv [Preprint]. 2021 Jan 26:2020.06.
    Insert
    SARS-CoV-2 3a protein (aa 42-275)
    Tags
    sfGFP-His
    Expression
    Insect
    Mutation
    aa 42-275
    Promoter
    Polyhedrin
    Available Since
    Aug. 5, 2020
    Availability
    Academic Institutions and Nonprofits only
    156425-plasmid-map-sequence-id-307748

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Showing: 1801 - 1850 of 5038 results