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5,046 results

Showing: 1111 - 1140 of 5046 results

  5. pKSB-618 U6-sgRNA2/3 GGAA AACA

    Plasmid
    #173674
    Purpose
    To clone and express a gRNA in Anopheles
    Depositor
    Eric Marois
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Insect
    Available Since
    Sept. 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    173674-plasmid-map-sequence-id-342962

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  8. pOPINO_F2

    Plasmid
    #171926
    Purpose
    Bacterial expression of nanobody VHH_F2, which binds to receptor binding domain of the spike protein of SARS-CoV-2
    Depositor
    Ray Owens
    Article
    Huo et al Nat Commun. 2021 Sep 22;12(1):5469. doi:
    Insert
    VHH_F2
    Tags
    His6
    Expression
    Bacterial, Insect, and Mamm…
    Available Since
    Sept. 23, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    171926-plasmid-map-sequence-id-338638

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  12. pLIB_3xFlag-His-nsp5CS-nsp9 (SARS-CoV-2)

    Plasmid
    #169177
    Purpose
    Baculoviral transfer vector to express SARS-CoV-2 nsp9 with nsp5-cleavable 3xFlag-His6 tag in insect cells
    Depositor
    John Diffley
    Article
    Milligan et al Biochem J. 2021 Jul 16;478(13):2499-2515
    Insert
    3xFlag-His-nsp5CS-nsp9 (ORF1ab SARS-CoV-2)
    Tags
    3xFlag-His6 (nsp5-cleavable)
    Expression
    Insect
    Mutation
    Codon optimised for insect cells (Spodoptera frug…
    Promoter
    Polyhedrin
    Available Since
    July 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    169177-plasmid-map-sequence-id-333223

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  13. pBIG1b_nsp7-His6-nsp8 (SARS-CoV-2)

    Plasmid
    #169180
    Purpose
    Baculoviral transfer vector to express nsp7-His6-nsp8 fusion protein (SARS-CoV-2) in insect cells
    Depositor
    John Diffley
    Article
    Bertolin et al Biochem J. 2021 Jul 16;478(13):2425-2443
    Insert
    nsp7-His6-nsp8 (ORF1ab Synthetic, SARS-CoV-2)
    Tags
    His6 (internal tag linking nsp7 and nsp8): nsp7-H…
    Expression
    Insect
    Mutation
    Codon optimised for insect cells (Spodoptera frug…
    Promoter
    Polyhedrin
    Available Since
    July 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    169180-plasmid-map-sequence-id-333998

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  14. pBIG2abc_nsp12-3xFlag/nsp7-His6-nsp8 (SARS-CoV-2)

    Plasmid
    #169183
    Purpose
    Baculoviral transfer vector to co-express nsp12-3xFlag and nsp7-His6-nsp8 fusion in insect cells
    Depositor
    John Diffley
    Article
    Bertolin et al Biochem J. 2021 Jul 16;478(13):2425-2443
    Insert
    nsp12-3xFlag/nsp7-His6-nsp8 (ORF1ab SARS-CoV-2)
    Tags
    3xFlag (nsp12 C-terminus) and His6 (as internal t…
    Expression
    Insect
    Mutation
    Codon optimised for insect cells (Spodoptera frug…
    Promoter
    Polyhedrin
    Available Since
    July 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    169183-plasmid-map-sequence-id-333983

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  15. pBIG2abc_nsp12-His6-3xFlag/nsp7-Linker-nsp8 (SARS-CoV-2)

    Plasmid
    #169184
    Purpose
    Baculoviral transfer vector to co-express nsp12-His6-3xFlag and nsp7-Linker-nsp8 fusion in insect cells
    Depositor
    John Diffley
    Article
    Bertolin et al Biochem J. 2021 Jul 16;478(13):2425-2443
    Insert
    nsp12-His6-3xFlag/nsp7-Linker-nsp8 (ORF1ab Synthetic, SARS-CoV-2)
    Tags
    His6-3xFlag (nsp12 C-terminus) and nsp7-nsp8 fusi…
    Expression
    Insect
    Mutation
    Codon optimised for insect cells (Spodoptera frug…
    Promoter
    Polyhedrin
    Available Since
    July 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    169184-plasmid-map-sequence-id-333981

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  22. 908H

    Plasmid
    #160240
    Purpose
    TetO(2x)-Hsp70min-UASpmin-Firefly-T2A-eGFP-p10-3’UTR-Ubiquitin-Renilla-attB
    Depositor
    Omar Akbari
    Article
    Gamez et al Insect Mol Biol. 2021 Oct;30(5):461-471.
    Inserts
    Tet Operator (2x)
    hsp70 minimal promoter
    p-element promoter
    Firefly luciferase
    Renilla luciferase
    Ubiquitin promoter
    Tags
    T2A GFP
    Expression
    Insect
    Available Since
    Nov. 23, 2020
    Availability
    Academic Institutions and Nonprofits only
    160240-plasmid-map-sequence-id-316017

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  23. pUAS-kkv::NG

    Plasmid
    #138953
    Purpose
    Used to generate a transgene that will express a fluorescent Chitin Synthase in Drosophila using Gal4
    Depositor
    Paul Adler
    Article
    Adler et al bioRxiv 2020
    Insert
    kkv (NEWENTRY Fly)
    Use
    Uas transgene vector
    Tags
    Neon Green fused to C terminus of Kkv
    Expression
    Insect
    Available Since
    Nov. 1, 2020
    Availability
    Academic Institutions and Nonprofits only
    138953-plasmid-map-sequence-id-274788

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Showing: 1111 - 1140 of 5046 results