4,908 results

1. pMT mCherry-CaaX

   Plasmid

   #126669

   Purpose

   Inducible expression of mCherry-CaaX in S2 cells

   Depositor

   Ron Vale

   Article

   Williamson et al J Cell Biol. 2018 Nov 5;217(11):3977-399

   Insert

   mCherry-CaaX
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Jan. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pE3n

   Plasmid

   #17457

   Purpose

   modified pENTR vector for N-terminal 6x MYC tag fusion with your gene of interest

   Depositor

   Giovanna Benvenuto

   Article

   Dubin et al Plant Methods. 2008 Jan 22. 4(1):3.

   Type

   Empty backbone

   Use

   Modified gateway entry vector

   Tags

   6xMyc

   Expression

   Bacterial, Insect, Mammalia…

   Mutation

   Promoter

   Available since

   March 21, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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3. pUAS-ERK-YFP

   Plasmid

   #42076

   Depositor

   Norbert Perrimon

   Article

   Friedman et al Nature. 2006 Nov 9;444(7116):230-4. Epub

   Insert

   Drosophila ERK ortholog (rl Fly)
   

   Use

   Tags

   YFP

   Expression

   Insect

   Mutation

   Promoter

   Hsp70

   Available since

   March 6, 2013

   Availability

   Academic Institutions and Nonprofits only

4. pUWR-CadTS-C

   Plasmid

   #58326

   Purpose

   Destination vector for inserting ubi-CadTS control to Drosophila melanogaster genome

   Depositor

   Denise Montell

   Article

   Cai et al Cell. 2014 May 22;157(5):1146-59. doi: 1

   Insert

   E-cadherin tension sensor control
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   poly-ubiquitin

   Available since

   Aug. 5, 2014

   Availability

   Academic Institutions and Nonprofits only

5. pExpreS2-1

   Plasmid

   #175360

   Purpose

   ZeoR backbone for recombinant protein overexpression with Drosophila ExpreS2 Platform

   Depositor

   ExpreS2ion Biotechnologies

   Article

   Coker et al Front Bioeng Biotechnol. 2022 May 5;10:8

   Type

   Empty backbone

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Fused Actin-HSP70

   Available since

   March 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pACUH-sfcherry2(1-10)

   Plasmid

   #172061

   Purpose

   UAS vector to express sfcherry2(1-10) in Drosophila

   Depositor

   Daichi Kamiyama

   Article

   Kamiyama et al Proc Natl Acad Sci U S A. 2021 Jun 8;118

   Insert

   sfcherry2(1-10)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Oct. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pFB-HTB-ASK1

   Plasmid

   #29517

   Depositor

   Ning Zheng

   Article

   Sheard et al Nature. 2010 Nov 18. 468(7322):400-5.

   Insert

   ASK1 (SKP1 Mustard Weed)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   April 14, 2011

   Availability

   Academic Institutions and Nonprofits only

8. pFBOH-MHL.SUZ12

   Plasmid

   #125172

   Purpose

   Expresses human SUZ12 in insect cells, under a TEV-cleavable N-terminal polyhistidine tag.

   Depositor

   Chen Davidovich

   Article

   Zhang et al Nat Struct Mol Biol. 2019 Mar;26(3):237-

   Insert

   SUZ12 (SUZ12 Human)
   

   Use

   Tags

   His-TEV

   Expression

   Insect

   Mutation

   Promoter

   Polyhedrin

   Available since

   May 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. IMPT-6213

   Plasmid

   #99333

   Purpose

   AT2R N terminal BRIL, linker GSGS, delta aa 1-34, delta aa 336-363

   Depositor

   Raymond Stevens

   Article

   Zhang et al Nature. 2017 Apr 20;544(7650):327-332. d

   Insert

   AGTR2 (AGTR2 Human)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   polyhedrin

   Available since

   Sept. 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. 10xUAS-GFP-DD

    Plasmid

    #104989

    Purpose

    Expresses GFP fused with destabilizing domain (DD) downstream of a 10XUAS sequence.

    Depositor

    Jing-W Wang

    Article

    Sethi et al Elife. 2017 Nov 15;6. pii: e30327. doi:

    Insert

    TMP-inducible destabilized GFP (GFP-DD)
    

    Use

    Tags

    DD

    Expression

    Insect

    Mutation

    Promoter

    Available since

    May 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pOPINTRX

    Plasmid

    #41116

    Depositor

    Ray Owens

    Article

    Bird et al Methods. 2011 Sep;55(1):29-37. Epub 2011

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    Promoter

    Available since

    March 27, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. p2Bac.His.XPB

    Plasmid

    #39216

    Depositor

    Aziz Sancar

    Insert

    XPB (ERCC3 Human)
    

    Use

    Tags

    His

    Expression

    Insect

    Mutation

    Promoter

    Pph

    Available since

    Aug. 24, 2012

    Availability

    Academic Institutions and Nonprofits only

13. pACEBac1-PARP1-Flag-His-7xKQ

    Plasmid

    #111573

    Purpose

    Expresses full length PARP1 with K to Q mutations in automodification domain in Sf9 (insect) cells

    Depositor

    Thomas Muir

    Article

    Liszczak et al Nat Chem Biol. 2018 Jul 16. pii: 10.1038

    Insert

    PARP1 (PARP1 Human)
    

    Use

    Tags

    C-terminal 6xHis and C-terminal Flag

    Expression

    Insect

    Mutation

    lysine to glutamine mutation at the following ami…

    Promoter

    polyhedrin promoter

    Available since

    July 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAc-y1sgRNA-Cas9

    Plasmid

    #49331

    Purpose

    Expresses sgRNA targeting yellow gene and Cas9-Puro in Drosophila S2 cells

    Depositor

    Ji-Long Liu

    Article

    Bassett et al Biol Open. 2013 Dec 10. pii: bio.2013712

    Inserts

    Cas9
    dU6-sgRNA
    y1 sgRNA (y Fly)
    

    Use

    CRISPR

    Tags

    3xFLAG and NLS

    Expression

    Insect

    Mutation

    Human codon optimised

    Promoter

    Actin-5c and Drosophila U6

    Available since

    Dec. 19, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. pOCC304

    Plasmid

    #118897

    Purpose

    shuttle vector for baculovirus production, using FlashBac bacmid; for rec. protein with N-terminal TwinStrep-MBP tag, cleavable with TEV and C-terminal HIS6, cleavable with TEV

    Depositor

    PEPC MPI CBG, Marc Bickle

    Article

    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

    Insert

    NcoI-TwinStrep-MBP-TEV-NotI-ccdB-AscI-TEV-HIS6-stop-HindIII cassette
    

    Use

    Tags

    HIS6, cleavable with TEVprotease and TwinStrep-MB…

    Expression

    Insect

    Mutation

    Promoter

    polH

    Available since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pScarlessHD-N-3xVHH05-DsRed

    Plasmid

    #171581

    Purpose

    For scarless insertion of 3xVHH05 NanoTag into N-term; Marked by the visible marker 3xP3-DsRed flanked by piggyBac termini.

    Depositor

    Norbert Perrimon, Hidde Ploegh

    Article

    Xu et al Elife. 2022 Jan 25;11. pii: 74326. doi:

    Insert

    3xVHH05 (UBE2J1 Human)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    June 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pFastBac1-Ac-AChBP

    Plasmid

    #63128

    Purpose

    Expression of Aplysia californica AChBP

    Depositor

    Titia Sixma

    Article

    Celie et al Nat Struct Mol Biol. 2005 Jul;12(7):582-

    Insert

    AChBP
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Polyhedrin

    Available since

    March 13, 2015

    Availability

    Academic Institutions and Nonprofits only

18. pTriEx-mVenus-PA-Rac1-T17N

    Plasmid

    #22017

    Purpose

    Expression of photoactivatable Rac1 (dominant-negative mutant)

    Depositor

    Klaus Hahn

    Article

    Wu et al Nature. 2009 Sep 3. 461(7260):104-8.

    Insert

    PA-Rac1-T17N (RAC1 Human, Avena sativa (oat))
    

    Use

    Tags

    6X His and mVenus

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    Rac1 starts at I4 and contains mutation T17N.

    Promoter

    CMV, p10

    Available since

    Sept. 29, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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19. p2Bac.His.XPD

    Plasmid

    #39217

    Depositor

    Aziz Sancar

    Insert

    XPD (ERCC2 Human)
    

    Use

    Tags

    His (HHYHHH)

    Expression

    Insect

    Mutation

    XPD starts at aa4

    Promoter

    Pph

    Available since

    Aug. 24, 2012

    Availability

    Academic Institutions and Nonprofits only

20. pFC31

    Plasmid

    #165845

    Purpose

    _C31 integrase CDS

    Depositor

    Koen Venken

    Article

    Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

    Insert

    _C31 integrase
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Sept. 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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