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Plasmid#71809PurposeGenerating transgenic flies for FLP-inducible DamIDDepositorInsertmin.hsp70P-FRT-STOP#1-FRT-Dam-Myc[open]
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEBFP2
Plasmid#165831PurposeEBFP2blue fluorescent protein CDSDepositorInsertEBFP2
UseTagsExpressionInsectMutationPromoterAvailable sinceSept. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKSB-610 U6-sgRNA2 GGAA AGAG
Plasmid#173672PurposeTo clone and express a gRNA in AnophelesDepositorTypeEmpty backboneUseCRISPRTagsExpressionInsectMutationPromoterAvailable sinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSLfa-PUb-EGFP-3'UTR#5
Plasmid#176337PurposeThe synthetic 3'UTR#5 for EGFP expression under the control of the Ae. aegypti polyubiquitin (PUb) promoterDepositorInsert3'UTR#5
UseTagsExpressionInsectMutationPromoterAvailable sinceJan. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pQUAST-frt-stop-frt-p65AD::CaM
Plasmid#64716Purposegenerating transgenic TRIC fliesDepositorInsertsp65AD::CaM
frt-stop-frt
UseTagsExpressionInsectMutationPromoterAvailable sinceJune 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSN840
Plasmid#184832PurposeExpresses human KIF5A(∆exon27) and human KLC1 in insect cellsDepositorUseCre/LoxTagsHis-FLAG and mScarlet-2xStrepIIExpressionInsectMutation∆exon27 form of human KIF5APromoterp10 and polyhedrinAvailable sinceJuly 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
nos-Cas9.874Z1
Plasmid#112685PurposeExpress hSpCas9 under nanos promoterDepositorInserthSpCas9
UseTags3x FLAG, EGFP, and NLSExpressionInsectMutationHumanized Cas9 bacterial sequencePromoternanosAvailable sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAc-y1sgRNA-Cas9
Plasmid#49331PurposeExpresses sgRNA targeting yellow gene and Cas9-Puro in Drosophila S2 cellsDepositorInsertsUseCRISPRTags3xFLAG and NLSExpressionInsectMutationHuman codon optimisedPromoterActin-5c and Drosophila U6Available sinceDec. 19, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFastBac1/MBP-His
Plasmid#38008DepositorInsertMBP (Maltose binding protein)
UseBaculovirus expressionTags6xHISExpressionInsectMutationPromoterPolyhedrinAvailable sinceOct. 1, 2012AvailabilityIndustry, Academic Institutions, and Nonprofits -
pMT-TRAF6-V5-His
Plasmid#63752PurposeExpresses drosophila TRAF6 tagged with V5 and 6xHis epitopes, under control of metallothionein promoterDepositorInsertTRAF6 (Traf6 Fly)
UseTagsV5-HisExpressionInsectMutationPromoterpMTAvailable sinceApril 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pE4n
Plasmid#17458Purposemodified pENTR vector for N-terminal GST tag fusion with your gene of interestDepositorTypeEmpty backboneUseModified gateway entry vectorTagsGST and Thrombin siteExpressionBacterial, Insect, Mammalia…MutationPromoterAvailable sinceMarch 21, 2008AvailabilityAcademic Institutions and Nonprofits only -
pCRISPaint-T2A-QF2-3xP3-RFP
Plasmid#127559PurposeCRISPaint universal donor plasmid for gene exon insertion. Encodes T2A-QF2-SV40 and 3xP3-RFP visible eye marker.DepositorInsertQF2
UseCRISPRTagsExpressionInsectMutationPromoterAvailable sinceSept. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMT-Flag-RELISH
Plasmid#63750PurposeExpresses drosophila RELISH tagged with a Flag epitopes, under control of metallothionein promoterDepositorInsertRelish (Rel Fly)
UseTagsFlagExpressionInsectMutationPromoterpMTAvailable sinceApril 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmG3BP_G
Plasmid#146349PurposeInsect Expression of DmG3BPDepositorInsertDmG3BP (rin Fly)
UseTagsExpressionInsectMutationV632G compared to AF231031.1, exact match to NP_5…PromoterAvailable sinceJan. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFastBacDual with LFn + Needle
Plasmid#84868PurposeBaculovirus Expression of LFn-Needle fusion. The LFn domain acts as a signal sequence that targets proteins for cytosolic translocation via the co-administered protective antigen (PA) channel proteinDepositorInsertsLFn
Needle
UseBaculovirusTags6xHisExpressionInsectMutationPromoterPolyhedrinAvailable sinceNov. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJFRC-20X-UAS-ADARcd
Plasmid#81173Purposeidentification of cell-specific targets of RNA binding proteinsDepositorInsertUAS-ADARcd
UseTagsExpressionInsectMutationPromoterUASAvailable sinceAug. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJFRC7-UAS-NPRR_ANP
Plasmid#133243PurposeFluorescent reporter for neuropeptide release imagingDepositorInsertrat atrial natriuretic peptide-fused codon-optimized GCaMP6s
UseTagsExpressionInsectMutationPromoterhsp70Available sinceFeb. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBac(3xP3-gTc’v; Tc-hsp68:Cre)
Plasmid#119063PurposeExpression of Cre recombinase in Tribolium castaneumDepositorInsertspBacL
Tc-hsp68 Promoter
Cre recombinase
Tc-hsp68 3'UTR
SV40polyA
Tc-vermilion
3xP3 TATA
pBacR
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pUAST-GFP-RpL10Ab
Plasmid#71729PurposeExpression of Drosophila melanogaster Rpl10Ab-GFP fusionDepositorInsertRpL10Ab (RpL10Ab Fly)
UseTagsEGFPExpressionInsectMutationPromoterhsp70Available sinceDec. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDest-636
Plasmid#159574PurposeGateway Destination vector for protein expression in baculovirusDepositorTypeEmpty backboneUseTagsHis6-MBPExpressionInsectMutationPromoterAvailable sinceOct. 5, 2020AvailabilityAcademic Institutions and Nonprofits only