4,931 results

1. pUAST-attB-yki:V5-S111A

   Plasmid

   #85619

   Purpose

   Expresses V5 tagged mutant yki in Drosophila.

   Depositor

   Kenneth Irvine

   Article

   Oh et al Oncogene. 2009 Apr 30;28(17):1916-27. do

   Insert

   yki-V5-S111A
   

   Tags

   V5

   Expression

   Insect

   Available Since

   May 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pUAST-attB-yki:V5-S250A

   Plasmid

   #85618

   Purpose

   Expresses V5 tagged mutant yki in Drosophila.

   Depositor

   Kenneth Irvine

   Article

   Oh et al Oncogene. 2009 Apr 30;28(17):1916-27. do

   Insert

   yki-V5-S250A
   

   Tags

   V5

   Expression

   Insect

   Available Since

   May 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pHW-CIBN-mCerulean-MP-HRW-CRY2-VHH(GFP)

   Plasmid

   #126025

   Purpose

   express RFP- and mCer-tagged LARIAT for optogenetic clustering

   Depositor

   Eurico Morais-de-Sá

   Article

   Osswald et al Biomolecules. 2019 Feb 12;9(2). pii: bio

   Insert

   CIBN-mCerulean-MP; CRY2 PHR-VHH(GFP)
   

   Tags

   RFP

   Expression

   Insect

   Mutation

   WT

   Promoter

   Hsp70

   Available Since

   Sept. 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. Pub-mCD8:GFP-T2A-DsRed:NLS-SV40

   Plasmid

   #130665

   Purpose

   Plasmid to test the efficiency of T2A multiplexing in Aedes aegypti

   Depositor

   Leslie Vosshall

   Article

   Matthews et al eLife. 2019 May 21;8. pii: 43963. doi: 1

   Insert

   AaegPUb-mCD8-GFP-T2A-dsRed-NLS-SV40
   

   Use

   Ae. aegypti expression

   Expression

   Insect

   Promoter

   Aedes aegypti poly-ubiquitin

   Available Since

   Oct. 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMTgR2

   Plasmid

   #107286

   Purpose

   Plasmid can be used for Drosophila S2 driven secretion of extracellular part of human interferon-_ receptor 2.

   Depositor

   Bohdan Schneider

   Article

   Mikulecky et al Acta Crystallogr D Struct Biol. 2016 Sep

   Insert

   Interferon gamma receptor 2 (IFNGR2 Human)
   

   Tags

   CATCATCACCATCACCATGA

   Expression

   Insect

   Promoter

   Metallothionein promoter

   Available Since

   March 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pUAST myc mouse Rab35 CA

   Plasmid

   #53516

   Depositor

   Matthew Scott

   Article

   Zhang et al Science. 2009 Sep 4;325(5945):1250-4. do

   Insert

   Rab35 (Rab35 Mouse)
   

   Tags

   myc

   Expression

   Insect

   Mutation

   Q67L

   Promoter

   GAL4

   Available Since

   June 4, 2014

   Availability

   Academic Institutions and Nonprofits only

7. pFastBac His HA GNU WT

   Plasmid

   #112994

   Purpose

   Baculovirus expression of His/HA-tagged Drosophila gnu

   Depositor

   Terry Orr-Weaver

   Article

   Hara et al Elife. 2017 May 30;6. doi: 10.7554/eLife

   Insert

   gnu (gnu Fly)
   

   Tags

   His/HA

   Expression

   Insect

   Available Since

   Aug. 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. BacPAK HF-Uch37

   Plasmid

   #19425

   Depositor

   Joan Conaway, Ronald Conaway

   Article

   Yao et al Nat Cell Biol. 2006 Sep . 8(9):994-1002.

   Insert

   Uch37 (UCHL5 Human)
   

   Tags

   Flag and His

   Expression

   Insect

   Available Since

   Nov. 21, 2008

   Availability

   Academic Institutions and Nonprofits only

9. pMCh-56

   Plasmid

   #92260

   Purpose

   Plasmid for expression of HA-tagged Drosophila melanogaster GW182 in D. melanogaster cells

   Depositor

   Marina Chekulaeva, Roy Parker

   Article

   Chekulaeva et al RNA. 2009 May;15(5):794-803. doi: 10.126

   Insert

   dGW182 (gw Fly)
   

   Tags

   HA

   Expression

   Insect

   Promoter

   Ac5

   Available Since

   Dec. 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. EKAR2G_design1_mTFP_105_Venus_229

    Plasmid

    #39822

    Depositor

    Olivier Pertz

    Article

    Fritz et al Sci Signal. 2013 Jul 23;6(285):rs12. doi

    Insert

    Erk activity reporter
    

    Tags

    His-Myc

    Expression

    Bacterial, Insect, and Mamm…

    Promoter

    CMV

    Available Since

    Oct. 22, 2012

    Availability

    Academic Institutions and Nonprofits only

11. LexAop2-myr::4xCLIPf

    Plasmid

    #87636

    Purpose

    For creating drosophila transgenics expressing LexAop2-myr4x::CLIPf

    Depositor

    Gregory Jefferis

    Article

    Sutcliffe et al Genetics. 2017 Apr;205(4):1399-1408. doi

    Insert

    myr::4xCLIPf
    

    Expression

    Insect

    Available Since

    March 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pENTR-GFP-Patronin CC 638–1457

    Plasmid

    #59051

    Purpose

    for baculovirus expression of Patronin CC truncation (using Baculodirect kit from Invitrogen)

    Depositor

    Ron Vale

    Article

    Hendershott et al Proc Natl Acad Sci U S A. 2014 Apr 22;11

    Insert

    Patronin (Patronin Fly)
    

    Tags

    eGFP

    Expression

    Bacterial and Insect

    Available Since

    Sept. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

13. pENTR-GFP-Patronin CC 776–1457

    Plasmid

    #59052

    Purpose

    for baculovirus expression of Patronin CC truncation (using Baculodirect kit from Invitrogen)

    Depositor

    Ron Vale

    Article

    Hendershott et al Proc Natl Acad Sci U S A. 2014 Apr 22;11

    Insert

    Patronin (Patronin Fly)
    

    Tags

    eGFP

    Expression

    Bacterial and Insect

    Available Since

    Sept. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

14. GRM1

    Plasmid

    #25353

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    GRM1-C254S:L28-G518 (GRM1 Human)
    

    Tags

    His

    Expression

    Insect

    Mutation

    C254S. Contains amino acids L28-G518.

    Available Since

    July 29, 2011

    Availability

    Industry, Academic Institutions, and Nonprofits

15. CJ29 (let-7 perfect site)

    Plasmid

    #17766

    Depositor

    David Bartel

    Article

    Ruby et al Nature. 2007 Jul 5. 448(7149):83-6.

    Insert

    let-7 perfect site (let-7 Fly)
    

    Use

    Luciferase; Drosophila metallothionein gene promo…

    Tags

    renilla luciferase

    Expression

    Insect

    Available Since

    April 15, 2008

    Availability

    Academic Institutions and Nonprofits only

16. pUASp YFP Rab7 CA

    Plasmid

    #37704

    Depositor

    Matthew Scott

    Article

    Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

    Insert

    Rab7 (Rab7 Fly)
    

    Use

    Drosophila

    Tags

    YFP

    Expression

    Insect

    Mutation

    Q67L (constitutive active)

    Promoter

    GAL4

    Available Since

    June 10, 2013

    Availability

    Academic Institutions and Nonprofits only

17. pUC 3GLA UAS HAi

    Plasmid

    #71763

    Purpose

    gap-repair recombineering for Drosophila transgenesis, contains loxP cassette with GFP, Amp resistant, with 5xUAS and HA tag

    Depositor

    Matthias Soller

    Article

    Haussmann et al Nucleic Acids Res. 2018 Dec 12. pii: 523

    Type

    Empty backbone

    Expression

    Insect

    Available Since

    Dec. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pOCC177

    Plasmid

    #118886

    Purpose

    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6-MBP tag, cleavable with 3C, and C-terminal SNAP tag, cleavable with TEV

    Depositor

    Marc Bickle, PEPC MPI CBG

    Article

    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

    Insert

    NcoI-HIS6-MBP-3C-NotI-ccdB-AscI-TEV-SNAP-stop-HindIII cassette
    

    Tags

    HIS6-MBP, cleavable with 3C protease and SNAP tag…

    Expression

    Insect

    Promoter

    polH

    Available Since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pOCC194

    Plasmid

    #118882

    Purpose

    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with C-terminal SNAP tag, cleavable with TEV, and C-terminal HIS6 tag, cleavable with 3C

    Depositor

    Marc Bickle, PEPC MPI CBG

    Article

    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

    Insert

    NcoI-NotI-ccdB-AscI-TEV-SNAP-3C-HIS6-stop-HindIII cassette
    

    Tags

    SNAP tag, cleavable with TEV protease, HIS6, clea…

    Expression

    Insect

    Promoter

    polH

    Available Since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pOCC230

    Plasmid

    #118871

    Purpose

    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6 tag, cleavable with 3C, and C-terminal mCitrine, cleavable with TEV

    Depositor

    Marc Bickle, PEPC MPI CBG

    Article

    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

    Insert

    NcoI-HIS6-3C--NotI-ccdB-AscI-TEV-mCitrine-stop-HindIII cassette
    

    Tags

    HIS6, cleavable with 3C protease and mCitrine, cl…

    Expression

    Insect

    Promoter

    polH

    Available Since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pOCC69

    Plasmid

    #118874

    Purpose

    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6 tag, cleavable with 3C, and C-terminal monomeric GFP, cleavable with TEV

    Depositor

    Marc Bickle, PEPC MPI CBG

    Article

    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

    Insert

    NcoI-HIS6-3C--NotI-ccdB-AscI-TEV-mGFP-stop-HindIII cassette
    

    Tags

    HIS6, cleavable with 3C protease and mGFP, cleava…

    Expression

    Insect

    Promoter

    polH

    Available Since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pOCC110

    Plasmid

    #118905

    Purpose

    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6, cleavable with 3C, and C-terminal sortase site (LPETGg)

    Depositor

    Marc Bickle, PEPC MPI CBG

    Article

    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

    Insert

    NcoI-HIS6-3C-NotI-ccdB-AscI-LPETGg-stop-HindIII cassette
    

    Tags

    HIS6, cleavable with 3C protease and LPETGg

    Expression

    Insect

    Promoter

    polH

    Available Since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pOCC210

    Plasmid

    #118896

    Purpose

    shuttle vector for baculovirus production, using FlashBac bacmid; for rec. protein with N-terminal MBP tag, cleavable with 3C and C-terminal SNAP, cleavable with TEV and HIS6, cleavable with 3C

    Depositor

    Marc Bickle, PEPC MPI CBG

    Article

    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

    Insert

    NcoI-MBP-3C-NotI-ccdB-AscI-TEV-SNAP-3C-HIS6-stop-HindIII cassette
    

    Tags

    MBP, cleavable with 3C protease and SNAP tag, cle…

    Expression

    Insect

    Promoter

    polH

    Available Since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pOCC231

    Plasmid

    #118885

    Purpose

    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6-MBP tag, cleavable with 3C, and C-terminal mCitrine, cleavable with TEV

    Depositor

    Marc Bickle, PEPC MPI CBG

    Article

    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

    Insert

    NcoI-HIS6-MBP-3C-NotI-ccdB-AscI-TEV-mCitrine-stop-HindIII cassette
    

    Tags

    HIS6-MBP, cleavable with 3C protease and mCitrine…

    Expression

    Insect

    Promoter

    polH

    Available Since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pOCC219

    Plasmid

    #118908

    Purpose

    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with C-terminal HIS10, cleavable with 3C protease and C-terminal YBBR tag

    Depositor

    Marc Bickle, PEPC MPI CBG

    Article

    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

    Insert

    NcoI-NotI-ccdB-AscI-YBBR-3C-HIS10-stop-HindIII cassette
    

    Tags

    YBBR tag, HIS10, cleavable with 3C protease

    Expression

    Insect

    Promoter

    polH

    Available Since

    Jan. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pXN-FBLWLF-VP16AD

    Plasmid

    #26268

    Depositor

    Tom Clandinin

    Article

    Gohl et al Nat Methods. 2011 Mar;8(3):231-7. doi: 1

    Insert

    VP16AD-ZIP+
    

    Use

    Drosophila transgenesis

    Expression

    Bacterial and Insect

    Available Since

    Feb. 2, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. EPHA2 (3MBW)

    Plasmid

    #25357

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    epha2.023.326 (EPHA2 Human)
    

    Tags

    His

    Expression

    Insect

    Available Since

    Aug. 19, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

28. pUASP Flag Rab32 CA

    Plasmid

    #44834

    Depositor

    Matthew Scott

    Insert

    Rab32 (Rab32 Fly)
    

    Use

    Drosophila

    Tags

    Flag

    Expression

    Insect

    Mutation

    Q79L (constitutive active)

    Available Since

    April 18, 2013

    Availability

    Academic Institutions and Nonprofits only

29. pUASP YFP Rab18 WT

    Plasmid

    #37717

    Depositor

    Matthew Scott

    Article

    Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

    Insert

    Rab18 (Rab18 Fly)
    

    Use

    Drosophila

    Tags

    YFP

    Expression

    Insect

    Promoter

    GAL4

    Available Since

    March 13, 2013

    Availability

    Academic Institutions and Nonprofits only

30. 10XUAS IVS Jaws mCherry_tr

    Plasmid

    #111550

    Purpose

    Optogenetics in Drosophila: UAS-induced expression of Jaws mCherry

    Depositor

    Vivek Jayaraman

    Article

    Chuong et al Nat Neurosci. 2014 Aug;17(8):1123-9. doi

    Insert

    Jaws_mCherry_trafficked
    

    Expression

    Insect

    Mutation

    wt

    Promoter

    hsp70

    Available Since

    June 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pJFRC81-3xVAS3-IVS-Syn21-GFP-P10

    Plasmid

    #104614

    Purpose

    Expresses GFP upon binding of TALE3 to VAS3

    Depositor

    Tudor Fulga

    Article

    Toegel et al Nat Commun. 2017 Nov 21;8(1):1663. doi:

    Insert

    3xVAS3
    

    Expression

    Insect

    Promoter

    hsp70

    Available Since

    March 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pOT2 3GLA

    Plasmid

    #71764

    Purpose

    gap-repair recombineering for Drosophila transgenesis, contains loxP cassette with GFP, chloramphenicol resistant

    Depositor

    Matthias Soller

    Article

    Haussmann et al Nucleic Acids Res. 2018 Dec 12. pii: 523

    Type

    Empty backbone

    Expression

    Insect

    Available Since

    Dec. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pCaSpeR-4 porcupine (genomic DNA)

    Plasmid

    #37376

    Depositor

    Norbert Perrimon

    Article

    Kadowaki et al Genes Dev. 1996 Dec 15;10(24):3116-28.

    Insert

    porcupine (por Fly)
    

    Expression

    Insect

    Available Since

    July 16, 2012

    Availability

    Academic Institutions and Nonprofits only

34. pMT mir-1-1 reporter cel-mir-40

    Plasmid

    #46739

    Depositor

    David Bartel

    Article

    Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

    Insert

    cel-mir-40
    

    Use

    RNAi

    Tags

    His and hsa-mir-1-1

    Expression

    Insect

    Promoter

    Metallothionein (MT)

    Available Since

    June 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. PGRP-LCa

    Plasmid

    #21059

    Depositor

    Johann Deisenhofer

    Article

    Chang et al Proc Natl Acad Sci U S A. 2005 Jul 19. 1

    Insert

    PGRP-LCa (PGRP-LF Fly)
    

    Tags

    His

    Expression

    Insect

    Available Since

    Sept. 8, 2009

    Availability

    Academic Institutions and Nonprofits only

36. pIDS-RESTmut full-length

    Plasmid

    #109162

    Purpose

    Encodes human full-length REST/NRSF C402S to be expressed in a baculovirus/insect cell expression system

    Depositor

    Monika Golas

    Article

    Inui et al Protein Sci. 2017 May;26(5):997-1011. do

    Insert

    human full-length REST/NRSF (REST Human)
    

    Expression

    Insect

    Mutation

    Cys402Ser

    Promoter

    p10

    Available Since

    Jan. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. Or74a-Gal4

    Plasmid

    #63048

    Purpose

    To express Gal4 in the pattern of the Drosophila melanogaster odorant receptor Or74a, using the promoter sequences of the gene cloned 5' of Gal4

    Depositor

    Leslie Vosshall

    Article

    Fishilevich et al Curr Biol. 2005 Dec 6;15(23):2086-96.

    Insert

    Drosophila melanogaster Or74a promoter
    

    Tags

    Drosophila ADH (aa's 1-18) fused to Yeast Ga…

    Expression

    Insect

    Available Since

    April 22, 2015

    Availability

    Academic Institutions and Nonprofits only

38. pUASp YFP Rab23 DN

    Plasmid

    #37727

    Depositor

    Matthew Scott

    Article

    Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

    Insert

    Rab23 (Rab23 Fly)
    

    Use

    Drosophila

    Tags

    YFP

    Expression

    Insect

    Mutation

    S51N (dominant negative)

    Promoter

    GAL4

    Available Since

    Nov. 20, 2012

    Availability

    Academic Institutions and Nonprofits only

39. pUASp YFP Rab23 CA

    Plasmid

    #37728

    Depositor

    Matthew Scott

    Article

    Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

    Insert

    Rab23 (Rab23 Fly)
    

    Use

    Drosophila

    Tags

    YFP

    Expression

    Insect

    Mutation

    Q96L (constitutive active)

    Promoter

    GAL4

    Available Since

    Nov. 20, 2012

    Availability

    Academic Institutions and Nonprofits only

40. pJFRC81-3xVAS1-IVS-Syn21-GFP-P10

    Plasmid

    #104613

    Purpose

    Expresses GFP upon binding of TALE1 to VAS1

    Depositor

    Tudor Fulga

    Article

    Toegel et al Nat Commun. 2017 Nov 21;8(1):1663. doi:

    Insert

    3xVAS1
    

    Expression

    Insect

    Promoter

    hsp70

    Available Since

    Jan. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge