4,908 results

1. pMT-Mad2-GFP

   Plasmid

   #24284

   Depositor

   Ron Vale

   Article

   Griffis et al J Cell Biol. 2007 Jun 18. 177(6):1005-15

   Insert

   Mad2 (mad2 Fly)
   

   Use

   Tags

   GFP

   Expression

   Insect

   Mutation

   Promoter

   Available since

   May 10, 2010

   Availability

   Academic Institutions and Nonprofits only

2. ORCO-T2A-QF2

   Plasmid

   #162524

   Purpose

   Plasmid for CRISPR-mediated knock-in line that expresses a QF2 transcriptional activator by knocking-in a T2A-QF2 fragment replacing the stop codon of the endogenous Aedes aegypti Orco gene

   Depositor

   Leslie Vosshall

   Article

   Herre et al Cell. 2022 Aug 18;185(17):3104-3123.e28.

   Insert

   Orco-left-HDR-arm; T2A-QF2-SV40; 3xP3-dsRed-SV40; Orco-right-HDR-arm
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Aug. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. 874U=ExuL-NLS-hSpCas9-T2A-GFP, Opie2-dsRed-SV40

   Plasmid

   #159671

   Purpose

   Plasmid supports Cas9 expression only in males, Opie2-dsRed tagged, and can be integrated with pBac or phC31.

   Depositor

   Omar Akbari

   Article

   Kandul et al Elife. 2021 Mar 5;10. pii: 65939. doi: 1

   Insert

   ExuL-NLS-hSpCas9-T2A-GFP
   

   Use

   Tags

   T2A-GFP

   Expression

   Insect

   Mutation

   Promoter

   Available since

   March 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pMT-HGP-v3-Nb127D01-hIgG

   Plasmid

   #171564

   Purpose

   Expresses hIgG-tagged anti-CXCR2 (human) recombinant llama nanobody (Nb127D01-human IgG-TEV-Avi-His) in fly cells

   Depositor

   Norbert Perrimon, Hidde Ploegh

   Article

   Xu et al Elife. 2022 Jan 25;11. pii: 74326. doi:

   Insert

   Nb127D01-human IgG-TEV-Avi-His (CXCR2 Human)
   

   Use

   Tags

   BiP signal peptide and hIgG1-hinge-N297A-TEV site…

   Expression

   Insect

   Mutation

   Promoter

   Available since

   June 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMT-HGP-v3-NbVHH05-hIgG

   Plasmid

   #171565

   Purpose

   Expresses hIgG-tagged anti-UBC6e (human) recombinant alpaca nanobody (NbVHH05-human IgG-TEV-Avi-His) in fly cells

   Depositor

   Norbert Perrimon, Hidde Ploegh

   Article

   Xu et al Elife. 2022 Jan 25;11. pii: 74326. doi:

   Insert

   NbVHH05-human IgG-TEV-Avi-His (UBE2J1 Human)
   

   Use

   Tags

   BiP signal peptide and hIgG1-hinge-N297A-TEV site…

   Expression

   Insect

   Mutation

   Promoter

   Available since

   June 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. Hy_pMT Laccase2 MCS-WT Split GFP

   Plasmid

   #69891

   Purpose

   Expresses a GFP circular RNA in Drosophila

   Depositor

   Jeremy Wilusz

   Article

   Kramer et al Genes Dev. 2015 Oct 8.

   Insert

   GFP, laccase2 (stw Fly)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Metallothionein Promoter (pMT)

   Available since

   Nov. 3, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pUASp-Egl-Trbo

   Plasmid

   #179993

   Purpose

   Expresses Egl fused at the C-terminus with TurboID. Expression is driven using the UAS-Gal4 system.

   Depositor

   Graydon Gonsalvez

   Article

   Baker et al Development. 2021 Nov 15;148(22). pii: 2

   Insert

   Egl-Trbo
   

   Use

   Tags

   TurboID

   Expression

   Insect

   Mutation

   Promoter

   UAS-Gal4

   Available since

   March 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pJC-TALE-hairy

   Plasmid

   #46145

   Depositor

   David Stern

   Article

   Crocker et al Nat Methods. 2013 Aug;10(8):762-7. doi:

   Type

   Empty backbone

   Use

   Tags

   Hairy Repression Domain

   Expression

   Insect

   Mutation

   Promoter

   hsp70

   Available since

   Aug. 2, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGL2-ptc-lu

   Plasmid

   #37678

   Depositor

   Philip Beachy

   Article

   Chen et al Cell. 1999 Aug 6;98(3):305-16.

   Insert

   ptc promoter (−758 to +130)
   

   Use

   Luciferase

   Tags

   luciferase

   Expression

   Insect

   Mutation

   Promoter

   ptc

   Available since

   Nov. 12, 2012

   Availability

   Academic Institutions and Nonprofits only

10. pOPINF Ta-sro1 WWE-PARP

    Plasmid

    #192550

    Purpose

    E. coli expression vector for Triticum aestivum sro1 WWE-PARP domains (amino acids 2-434)

    Depositor

    Lennart Wirthmueller

    Article

    Vogt et al Plant Cell. 2022 Aug 18. pii: 6671231. d

    Insert

    SIMILAR TO RCD ONE 1 (SRO1) WWE-PARP domains, cultivar Shanrong No. 3 (SR3)
    

    Use

    Tags

    His6, 3C protease cleavage site

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    Promoter

    T7 promoter

    Available since

    Dec. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pRB37

    Plasmid

    #72869

    Purpose

    template for N-terminal Strep-8xHis-Strep-TEV site tag, constitutive Puromycin resistance

    Depositor

    Klaus Foerstemann

    Article

    Kunzelmann et al G3 (Bethesda). 2016 Jun 1;6(6):1777-85.

    Insert

    Strep - His8 - Strep -TEV tag
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    March 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pUASP dsRed Rab11 CA

    Plasmid

    #44830

    Depositor

    Matthew Scott

    Article

    Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

    Insert

    Rab11 (Rab11 Fly)
    

    Use

    Drosophila

    Tags

    dsRed

    Expression

    Insect

    Mutation

    Q70L (constitutive active)

    Promoter

    Available since

    April 18, 2013

    Availability

    Academic Institutions and Nonprofits only

13. pVMG0146_Cq-U6-1_2xBbsI-gRNA

    Plasmid

    #169238

    Purpose

    Expression of gRNA in Culex quinquefasciatus or other mosquitoes

    Depositor

    Valentino Gantz

    Article

    Feng et al Nat Commun. 2021 May 20;12(1):2960. doi:

    Insert

    Cq-U6-1_2xBbsI-gRNA
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    CPIJ039653

    Available since

    May 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pVMG0149_Cq-U6-6_2xBbsI-gRNA

    Plasmid

    #169323

    Purpose

    Expression of gRNA in Culex quinquefasciatus or other mosquitoes

    Depositor

    Valentino Gantz

    Article

    Feng et al Nat Commun. 2021 May 20;12(1):2960. doi:

    Insert

    pVMG0149_Cq-U6-6_2xBbsI-gRNA
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    CPIJ039596

    Available since

    May 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pTriEx4-ADCY6-C2 (917-1165 aa)

    Plasmid

    #113904

    Purpose

    His-S tagged cytoplasmic catalytic domain 2 of Adenylate Cyclase 6

    Depositor

    Anjaparavanda Naren

    Article

    Thomas et al J Biol Chem. 2018 Jun 14. pii: RA118.003

    Insert

    Adenylate Cyclase 6 cytoplasmic catalytic domain 2 (ADCY6 Human)
    

    Use

    Tags

    His-S

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    Please see Depositor Comments

    Promoter

    T7, CMV

    Available since

    Nov. 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pTriEx4-ADCY6-C1(306-672 aa)

    Plasmid

    #113903

    Purpose

    His-S tagged cytoplasmic catalytic domain 1 of Adenylate Cyclase 6

    Depositor

    Anjaparavanda Naren

    Article

    Thomas et al J Biol Chem. 2018 Jun 14. pii: RA118.003

    Insert

    Adenylate Cyclase 6 cytoplasmic catalytic domain 1 (ADCY6 Human)
    

    Use

    Tags

    His-S

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    Promoter

    T7, CMV

    Available since

    Oct. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. CJ52 (cg1849 utr mut)

    Plasmid

    #17780

    Depositor

    David Bartel

    Article

    Ruby et al Nature. 2007 Jul 5. 448(7149):83-6.

    Insert

    cg1849 utr fragment mut
    

    Use

    Luciferase; Drosophila metallothionein gene promo…

    Tags

    renilla luciferase

    Expression

    Insect

    Mutation

    mutant version

    Promoter

    Available since

    April 15, 2008

    Availability

    Academic Institutions and Nonprofits only

18. pmCD8-GFP,y+

    Plasmid

    #24350

    Depositor

    Liqun Luo

    Article

    Potter et al Cell. 2010 Apr 30. 141(3):536-48.

    Insert

    Yellow gene
    

    Use

    Tags

    CD8-GFP

    Expression

    Insect

    Mutation

    Promoter

    Available since

    April 12, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. BacPAK Flag-NFRKB

    Plasmid

    #19791

    Depositor

    Ronald Conaway, Joan Conaway

    Article

    Yao et al Mol Cell. 2008 Sep 26. 31(6):909-17.

    Insert

    NFRKB (NFRKB Human)
    

    Use

    Tags

    Flag and His

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Dec. 15, 2008

    Availability

    Academic Institutions and Nonprofits only

20. pJET1.2[eb-Tc’bhsp-EGFP-2A-Cre; ye-3xP3-gTc’v-SV40]

    Plasmid

    #124069

    Purpose

    CRISPR/Cas mediated knock-in via non-homologous end-joining in Tribolium; bhsp drives EGFP expression; Dm-ebony and Dm-yellow for linearization; (Transformation plasmid; Black eye marker)

    Depositor

    Gregor Bucher

    Insert

    eb-Tc’bhsp-EGFP-2A-Cre; ye-3xP3-gTc’v-SV40
    

    Use

    CRISPR and Cre/Lox

    Tags

    Expression

    Insect

    Mutation

    Promoter

    bhsp68

    Available since

    July 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge