5,046 results
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Plasmid#118874Purposeshuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6 tag, cleavable with 3C, and C-terminal monomeric GFP, cleavable with TEVDepositorInsertNcoI-HIS6-3C--NotI-ccdB-AscI-TEV-mGFP-stop-HindIII cassette
TagsHIS6, cleavable with 3C protease and mGFP, cleava…ExpressionInsectPromoterpolHAvailable SinceJan. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOCC110
Plasmid#118905Purposeshuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6, cleavable with 3C, and C-terminal sortase site (LPETGg)DepositorInsertNcoI-HIS6-3C-NotI-ccdB-AscI-LPETGg-stop-HindIII cassette
TagsHIS6, cleavable with 3C protease and LPETGgExpressionInsectPromoterpolHAvailable SinceJan. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOCC207
Plasmid#118869Purposeshuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6 tag, cleavable with 3C, and N-terminal mCherryDepositorInsertNcoI-HIS6-3C-mCherry-NotI-ccdB-AscI-stop-HindIII cassette
TagsHIS6, cleavable with 3C protease, mCherryExpressionInsectPromoterpolHAvailable SinceJan. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOCC210
Plasmid#118896Purposeshuttle vector for baculovirus production, using FlashBac bacmid; for rec. protein with N-terminal MBP tag, cleavable with 3C and C-terminal SNAP, cleavable with TEV and HIS6, cleavable with 3CDepositorInsertNcoI-MBP-3C-NotI-ccdB-AscI-TEV-SNAP-3C-HIS6-stop-HindIII cassette
TagsMBP, cleavable with 3C protease and SNAP tag, cle…ExpressionInsectPromoterpolHAvailable SinceJan. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOCC231
Plasmid#118885Purposeshuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6-MBP tag, cleavable with 3C, and C-terminal mCitrine, cleavable with TEVDepositorInsertNcoI-HIS6-MBP-3C-NotI-ccdB-AscI-TEV-mCitrine-stop-HindIII cassette
TagsHIS6-MBP, cleavable with 3C protease and mCitrine…ExpressionInsectPromoterpolHAvailable SinceJan. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOCC81
Plasmid#118900Purposeshuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6, cleavable with 3C , and N-terminal GSTDepositorInsertNcoI-HIS6-3C-GST-NotI-ccdB-AscI-stop-HindIII cassette
TagsHIS6, cleavable with 3C protease; GST tagExpressionInsectPromoterpolHAvailable SinceJan. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOCC254
Plasmid#118889Purposeshuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6-MBP tag, cleavable with 3C, and N-terminal mCherry, cleavable with TEVDepositorInsertNcoI-HIS6-MBP-3C-mCherry-TEV-NotI-ccdB-AscI-stop-HindIII cassette
TagsHIS6-MBP, cleavable with 3C protease; mCherry, cl…ExpressionInsectPromoterpolHAvailable SinceJan. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOCC215
Plasmid#118879Purposeshuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with C-terminal Dendra2, and C-terminal HIS6 tag, cleavable with 3CDepositorInsertNcoI-NotI-ccdB-AscI-Dendra2-3C-HIS6-stop-HindIII cassette
TagsDendra2, HIS6, cleavable with 3C proteaseExpressionInsectPromoterpolHAvailable SinceJan. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOCC219
Plasmid#118908Purposeshuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with C-terminal HIS10, cleavable with 3C protease and C-terminal YBBR tagDepositorInsertNcoI-NotI-ccdB-AscI-YBBR-3C-HIS10-stop-HindIII cassette
TagsYBBR tag, HIS10, cleavable with 3C proteaseExpressionInsectPromoterpolHAvailable SinceJan. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pUC 3GLA UAS HAi
Plasmid#71763Purposegap-repair recombineering for Drosophila transgenesis, contains loxP cassette with GFP, Amp resistant, with 5xUAS and HA tagDepositorTypeEmpty backboneExpressionInsectAvailable SinceDec. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pOT2 3GLA
Plasmid#71764Purposegap-repair recombineering for Drosophila transgenesis, contains loxP cassette with GFP, chloramphenicol resistantDepositorTypeEmpty backboneExpressionInsectAvailable SinceDec. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFastBac-His6-Mps1-KD
Plasmid#114064Purposeprotein productionDepositorAvailable SinceSept. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFastBac-LacI-LAP
Plasmid#114034PurposeProduction of baculovirus with LacI-LAPDepositorInsertLacI-LAP
ExpressionInsectMutationnonePromoterCMVAvailable SinceAug. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFastBac His HA GNU WT
Plasmid#112994PurposeBaculovirus expression of His/HA-tagged Drosophila gnuDepositorAvailable SinceAug. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
Hook3(1-522)-GFP-Psc-StrepII
Plasmid#111861PurposeC-terminal GFP tag on Hook3 (amino acids 1-522) for expression in Sf9 cells. C-terminal GFP-Psc-StrepII tag.DepositorInsertHook3(1-552)-GFP (HOOK3 Human)
TagsGFP, Psc tag, and Strep tagExpressionInsectMutationOptimised for expression in SF9 cellsAvailable SinceJuly 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
20XUAS IVS Jaws mVenus_tr
Plasmid#111552PurposeOptogenetics in Drosophila: UAS-induced expression of Jaws mVenusDepositorInsertJaws_mVenus_tr
ExpressionInsectMutationwtPromoterhsp70Available SinceJune 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
10XUAS IVS Jaws mVenus_tr
Plasmid#111553PurposeOptogenetics in Drosophila: UAS-induced expression of Jaws mVenusDepositorInsertJaws_mVenus_tr
ExpressionInsectMutationwtPromoterhsp70Available SinceJune 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUAST-HA-dAtg13
Plasmid#111802PurposeInsect Expression of dAtg13DepositorAvailable SinceJune 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
ACVR1 (ACVR1Z-c041)
Plasmid#110269PurposeBaculovirus expression for structure determination; may not be full ORFDepositorAvailable SinceJune 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
ULK3 (ULK3A-c002)
Plasmid#110272PurposeBaculovirus expression for structure determination; may not be full ORFDepositorAvailable SinceJune 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
Hy_pMT dati MCS circfirefly_1677
Plasmid#110118PurposeExpresses a 1677-nt firefly luciferase circular RNA in Drosophila cellsDepositorInsertFirefly luciferase
ExpressionInsectPromoterMetallothionein Promoter (pMT)Available SinceMay 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGS_N_3xFLAG
Plasmid#101654PurposeExpression of type I signal-peptide containing proteins with N-terminal 3xFLAG-tag in insect cellsDepositorInsertLRP6 Signal peptide, KpnI linker, 3xFLAG tag
Tags3xFLAG tagExpressionInsectAvailable SinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMTgR2
Plasmid#107286PurposePlasmid can be used for Drosophila S2 driven secretion of extracellular part of human interferon-_ receptor 2.DepositorInsertInterferon gamma receptor 2 (IFNGR2 Human)
TagsCATCATCACCATCACCATGAExpressionInsectPromoterMetallothionein promoterAvailable SinceMarch 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJFRC81-3xVAS3-IVS-Syn21-GFP-P10
Plasmid#104614PurposeExpresses GFP upon binding of TALE3 to VAS3DepositorInsert3xVAS3
ExpressionInsectPromoterhsp70Available SinceMarch 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJC-ase0.8kb-TALE3-VP64-P10
Plasmid#104610PurposeExpresses TALE3 under the control of a 0.8kb ase enhancer elementDepositorAvailable SinceFeb. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJC-20xUAS-TALE1-VP64-P10
Plasmid#104606PurposeExpresses TALE1 under UAS controlDepositorInsertTALE1
UseTALENExpressionInsectPromoterhsp70Available SinceJan. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJC-mhc2.4kb-TALE3-VP64-P10
Plasmid#104611PurposeExpresses TALE3 under the control of a 2.4kb mhc enhancer elementDepositorAvailable SinceJan. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJFRC81-3xVAS1-IVS-Syn21-GFP-P10
Plasmid#104613PurposeExpresses GFP upon binding of TALE1 to VAS1DepositorInsert3xVAS1
ExpressionInsectPromoterhsp70Available SinceJan. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUASt-FMW-attB-AthHen1
Plasmid#104958PurposeExpression of FLAG Myc tagged A.thaliana-Hen1 (codon optimised for Drosophila) in Drosophila using somatic tissue Gal4 driversDepositorAvailable SinceJan. 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMCh-56
Plasmid#92260PurposePlasmid for expression of HA-tagged Drosophila melanogaster GW182 in D. melanogaster cellsDepositorAvailable SinceDec. 11, 2017AvailabilityAcademic Institutions and Nonprofits only