4,908 results

1. HBM-mVEGF-C-HisTag-pENTR

   Plasmid

   #64066

   Purpose

   Plasmid encodes mature form of murine VEGF-C preceded by honey bee melittin secretion signal; entry vector to Gateway cloning system.

   Depositor

   Joanna Bereta

   Article

   Klaus et al Protein Expr Purif. 2015 Mar 7. pii: S10

   Insert

   vascular endothelial growth factor C (Vegfc Mouse)
   

   Use

   Tags

   His-tag

   Expression

   Insect

   Mutation

   Promoter

   Available since

   April 24, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSGE_768_Nhe2-EcR_dCP

   Plasmid

   #71223

   Purpose

   Luciferase reporter

   Depositor

   Alexander Stark

   Article

   Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

   Insert

   768_Nhe2-EcR_dCP
   

   Use

   Luciferase

   Tags

   luciferase

   Expression

   Insect

   Mutation

   Promoter

   DSCP Drosophila synthetic core promoter

   Available since

   Dec. 16, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pSGE_769_DipB-EcR_dCP

   Plasmid

   #71222

   Purpose

   Luciferase reporter

   Depositor

   Alexander Stark

   Article

   Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

   Insert

   769_DipB-EcR_dCP
   

   Use

   Luciferase

   Tags

   luciferase

   Expression

   Insect

   Mutation

   Promoter

   DSCP Drosophila synthetic core promoter

   Available since

   Nov. 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. CG4845/pMT/V5-HisB

   Plasmid

   #17589

   Depositor

   Denise Montell

   Insert

   CG4845 (psidin Fly)
   

   Use

   Tags

   6x His and V5

   Expression

   Insect

   Mutation

   Promoter

   Available since

   April 3, 2008

   Availability

   Academic Institutions and Nonprofits only

5. pBGRY

   Plasmid

   #61599

   Purpose

   Transformation vector pBGRY

   Depositor

   John Carlson

   Article

   Koh et al Neuron. 2014 Aug 20;83(4):850-65. doi: 1

   Type

   Empty backbone

   Use

   Drosophila transformation vector

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Feb. 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pUASP YFP Rab4 WT

   Plasmid

   #37690

   Depositor

   Matthew Scott

   Article

   Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

   Insert

   Rab4 (Rab4 Fly)
   

   Use

   Drosophila

   Tags

   YFP

   Expression

   Insect

   Mutation

   Promoter

   GAL4

   Available since

   June 10, 2013

   Availability

   Academic Institutions and Nonprofits only

7. pUASP dsRed Rab5 DN

   Plasmid

   #44823

   Depositor

   Matthew Scott

   Article

   Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

   Insert

   Rab5 (Rab5 Fly)
   

   Use

   Drosophila

   Tags

   dsRed

   Expression

   Insect

   Mutation

   S43N (dominant negative)

   Promoter

   Available since

   April 18, 2013

   Availability

   Academic Institutions and Nonprofits only

8. pOPINTolB

   Plasmid

   #41138

   Depositor

   Ray Owens

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial, Insect, and Mamm…

   Mutation

   Promoter

   Available since

   March 27, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pUASp YFP Rab4 CA

   Plasmid

   #53466

   Depositor

   Matthew Scott

   Article

   Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

   Insert

   Rab04
   

   Use

   Tags

   YFP

   Expression

   Insect

   Mutation

   Q67L

   Promoter

   GAL4

   Available since

   June 4, 2014

   Availability

   Academic Institutions and Nonprofits only

10. pBIG2ab_nsp14/nsp10-6His-3xFlag (SARS-CoV-2)

    Plasmid

    #169164

    Purpose

    Baculoviral transfer vector to co-express SARS-CoV-2 nsp14 and nsp10 in insect cells

    Depositor

    John Diffley

    Article

    Canal et al Biochem J. 2021 Jul 16;478(13):2445-2464

    Insert

    nsp14/nsp10-6His-3xFlag (ORF1ab Synthetic, SARS-CoV-2)
    

    Use

    Tags

    6His-3xFlag (nsp10 C-terminus)

    Expression

    Insect

    Mutation

    Codon optimised for insect cells (Spodoptera frug…

    Promoter

    Polyhedrin

    Available since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pBIG1a_3xFlag-6His-nsp15 (SARS-CoV-2)

    Plasmid

    #169166

    Purpose

    Baculoviral transfer vector for the expression of SARS-CoV-2 nsp15 in insect cells

    Depositor

    John Diffley

    Article

    Canal et al Biochem J. 2021 Jul 16;478(13):2465-2479

    Insert

    3xFlag-6His-nsp15 (ORF1ab Synthetic, SARS-CoV-2)
    

    Use

    Tags

    3xFlag-6His

    Expression

    Insect

    Mutation

    Codon optimised for insect cells (Spodoptera frug…

    Promoter

    Polyhedrin

    Available since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pIE2-AD

    Plasmid

    #21603

    Depositor

    Takahiro Kusakabe

    Article

    Mon et al Anal Biochem. 2009 Sep 15;392(2):180-2.

    Insert

    NF-kappa-B p65 subunit (Rela Mouse)
    

    Use

    Gateway destination vector

    Tags

    Flag, Gateway destination cassette, and NLS

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Sept. 22, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pAc5.1B-EGFP-DmNot1_J

    Plasmid

    #146709

    Purpose

    Insect Expression of DmNot1

    Depositor

    Elisa Izaurralde

    Article

    Huntzinger et al Nucleic Acids Res. 2013 Jan;41(2):978-94

    Insert

    DmNot1 (Not1 Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Three non silent mutations N605S, K759M, G1999S a…

    Promoter

    Available since

    May 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pAW-EGFP-4xlet-7 (pAW-AS27)

    Plasmid

    #40834

    Purpose

    miRNA sensor used in Drosophila cell lines

    Depositor

    Phillip Zamore

    Article

    Ameres et al Science. 2010 Jun 18;328(5985):1534-9.

    Insert

    Four target sites perfectly complementary to let-7
    

    Use

    Mirna reporter

    Tags

    EGFP

    Expression

    Insect

    Mutation

    Promoter

    Actin5C

    Available since

    Sept. 4, 2013

    Availability

    Academic Institutions and Nonprofits only

15. pQUAST-p65AD::CaM

    Plasmid

    #64715

    Purpose

    generating transgenic TRIC flies

    Depositor

    Liqun Luo

    Article

    Gao et al Nat Neurosci. 2015 May 11. doi: 10.1038/

    Insert

    p65AD::CaM
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    May 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSLfa[Hsp_p-GAL4Delta-SV40;attp]fa

    Plasmid

    #86451

    Purpose

    Gal4 driver construct for misexpression in Tribolium with Tribolium basal promoter; has been used for enhancer trapping; (Shuttle plasmid)

    Depositor

    Gregor Bucher

    Insert

    Hsp_p-GAL4Delta-SV40;attp
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    March 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. RP78 pUAST Orco mRNA

    Plasmid

    #53431

    Purpose

    Expresses the full-length Drosophila melanogaster Orco cDNA under Gal4/UAS control

    Depositor

    Leslie Vosshall

    Article

    Benton et al PLoS Biol. 2006 Feb;4(2):e20. Epub 2006

    Insert

    Orco (Orco )
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Aug. 12, 2014

    Availability

    Academic Institutions and Nonprofits only

18. pACEBac1-NEDD1

    Plasmid

    #178073

    Purpose

    Insect cell expression of NEDD1

    Depositor

    Tarun Kapoor

    Article

    Wieczorek et al J Cell Biol. 2021 Mar 1;220(3). pii: 211

    Insert

    NEDD1 (NEDD1 Human)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Jan. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. S

    Plasmid

    #167172

    Purpose

    Expresses the full Leptodactylus latrans Na+K+ATPase with ATP1A1-S variant in Sf9 cells

    Depositor

    Susanne Dobler

    Article

    Mohammadi et al Curr Biol. 2021 Jun 21;31(12):2530-2538.

    Inserts

    ATP1A1-S
    ATP1B1
    

    Use

    Tags

    Expression

    Bacterial and Insect

    Mutation

    Promoter

    P10 and PH

    Available since

    Feb. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCFD3-MS2tail-SAM

    Plasmid

    #78905

    Purpose

    Vector for expressing a single sgRNA with SAM MS2 tail, under U6:3 promoter

    Depositor

    Norbert Perrimon

    Article

    Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Aug. 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge