4,908 results

1. pCFD3-ebony

   Plasmid

   #83380

   Purpose

   Drosophila gRNA expression plasmid targets ebony

   Depositor

   Richard Padgett

   Article

   Kane et al G3 (Bethesda). 2017 Jan 5;7(1):87-93. do

   Insert

   ebony (e Fly)
   

   Use

   CRISPR

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Oct. 28, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. nos-Cas9.874Z1

   Plasmid

   #112685

   Purpose

   Express hSpCas9 under nanos promoter

   Depositor

   Omar Akbari

   Article

   Kandul et al Nat Commun. 2019 Jan 8;10(1):84. doi: 10

   Insert

   hSpCas9
   

   Use

   Tags

   3x FLAG, EGFP, and NLS

   Expression

   Insect

   Mutation

   Humanized Cas9 bacterial sequence

   Promoter

   nanos

   Available since

   Jan. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pJFRC81-10XUAS-IVS-Syn21-GFP-p10

   Plasmid

   #36432

   Depositor

   Gerald Rubin

   Article

   Pfeiffer et al Proc Natl Acad Sci U S A. 2012 Apr 9.

   Insert

   GFP
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   May 3, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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4. pFLAG-XBP1u-FLuc

   Plasmid

   #31239

   Depositor

   Jimtong Horng

   Article

   Jheng et al Cell Microbiol. 2010 Jun . 12(6):796-813

   Insert

   FLAG-XBP1u-FLuc (XBP1 Human)
   

   Use

   Tags

   Flag and Fluc

   Expression

   Bacterial, Insect, and Mamm…

   Mutation

   Promoter

   Available since

   Aug. 16, 2011

   Availability

   Academic Institutions and Nonprofits only

5. IMPT-10316:GABR1

   Plasmid

   #157850

   Purpose

   HA-TEV-VFT-TMD-CC-PreX-eGFP in pFastBac1 for Sf9 expression

   Depositor

   Vadim Cherezov, Raymond Stevens

   Article

   Shaye et al Nature. 2020 Jun 17. pii: 10.1038/s41586

   Insert

   GABR1 (GABBR1 Human)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Polyhedrin

   Available since

   Aug. 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pKM19

   Plasmid

   #123655

   Purpose

   Encodes firefly luciferase under the pIEx promoter for constitutive expression in insect (e.g. Drosophila, mosquito) cells

   Depositor

   Kevin Maringer

   Article

   Fredericks et al PLoS Negl Trop Dis. 2019 Nov 6;13(11):e0

   Insert

   Firefly luciferase
   

   Use

   Luciferase

   Tags

   Expression

   Insect

   Mutation

   Promoter

   pIEx

   Available since

   May 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pFastBac Dual His6 MBP N10 TEV LIC cloning vector (5C)

   Plasmid

   #30123

   Depositor

   Scott Gradia

   Type

   Empty backbone

   Use

   Tags

   His6-MBP-N10-TEV

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Aug. 17, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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8. pFasBac+GFP-CAMSAP2

   Plasmid

   #59037

   Purpose

   for baculovirus expression of CAMSAP2 in Sf9 cells

   Depositor

   Ron Vale

   Article

   Hendershott et al Proc Natl Acad Sci U S A. 2014 Apr 22;11

   Insert

   CAMSAP2 (CAMSAP2 Human)
   

   Use

   Tags

   His and eGFP

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Sept. 8, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pFastBac1-melHis10TEV

   Plasmid

   #159428

   Purpose

   empty pFastBac1 secretion vector (polyhedrin promoter) adds N-term melittin signal sequence & TEV-protease-removable His10; avoids unwanted residues in final protein by using BseRI restriction sites

   Depositor

   Debra Hansen

   Type

   Empty backbone

   Use

   Tags

   melittin signal sequence (removed in vivo by sign…

   Expression

   Insect

   Mutation

   Promoter

   polyhedrin

   Available since

   June 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTP288

    Plasmid

    #113460

    Purpose

    human Flag tagged Nbs1 in baculovirus transfer vector

    Depositor

    Tanya Paull

    Article

    Lee et al J Biol Chem. 2013 May 3;288(18):12840-51

    Insert

    Nbs1 (NBN Human)
    

    Use

    Tags

    Flag

    Expression

    Insect

    Mutation

    Promoter

    polyhedrin

    Available since

    Sept. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBPnlsLexA::p65Uw

    Plasmid

    #26230

    Depositor

    Gerald Rubin

    Article

    Pfeiffer et al Genetics. 2010 Oct;186(2):735-55. doi: 1

    Insert

    nlsLexA::p65 (RELA Human)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Jan. 5, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTriEx-mCerulean-PA-Rac1

    Plasmid

    #22030

    Purpose

    Expression of photoactivatable Rac1

    Depositor

    Klaus Hahn

    Article

    Wu et al Nature. 2009 Sep 3. 461(7260):104-8.

    Insert

    PA-Rac1 (RAC1 Avena sativa (oat), Human)
    

    Use

    Tags

    6X His and mCerulean

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    Rac1 starts at I4 and contains mutations Q61L, E9…

    Promoter

    CMV, p10

    Available since

    Sept. 29, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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13. pUCDMp10

    Plasmid

    #170461

    Purpose

    EarlyBac insect cell expression system

    Depositor

    Hiro Furukawa

    Article

    Chou et al Cell. 2020 Jul 23;182(2):357-371.e13. do

    Insert

    New vector
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    March 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pIEx4-Cre

    Plasmid

    #198280

    Purpose

    Expresses Cre in insect cells

    Depositor

    Feng Zhang

    Article

    Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

    Insert

    pIEx4-Cre
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    March 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pE2n

    Plasmid

    #17456

    Purpose

    modified pENTR vector for N-terminal 3x HA tag fusion with your gene of interest

    Depositor

    Giovanna Benvenuto

    Article

    Dubin et al Plant Methods. 2008 Jan 22. 4(1):3.

    Type

    Empty backbone

    Use

    Modified gateway entry vector

    Tags

    3xHA

    Expression

    Bacterial, Insect, Mammalia…

    Mutation

    Promoter

    Available since

    March 21, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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16. pKM7

    Plasmid

    #154164

    Purpose

    mCherry expressed under the IE1 promoter and hr5 enhancer from Autographa californica nuclear polyhedris virus.

    Depositor

    Kevin Maringer

    Article

    Russell et al PLoS Negl Trop Dis. 2021 Feb 16;15(2):e0

    Insert

    mCherry
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    IE1 promoter and hr5 enhancer from Autographa cal…

    Available since

    Aug. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pKRG3-mU6-PUb-hSpCas9-pAc

    Plasmid

    #162163

    Purpose

    Expresses hSpCas9-T2A-pAc (Ae. aegypti PUb promoter) with cloning site for sgRNAs (Ae. aegypti U6 promoter); puromycin selectable

    Depositor

    Charles Rice

    Article

    Rozen-Gagnon et al Sci Rep. 2021 Jan 12;11(1):736. doi: 10.

    Inserts

    hSpCas9
    sgRNA
    

    Use

    CRISPR

    Tags

    T2A-pAc

    Expression

    Insect

    Mutation

    Promoter

    Ae. aegypti PUb and Ae. aegypti U6

    Available since

    Jan. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pVL1393-6xMyc

    Plasmid

    #128035

    Purpose

    Baculovirus expression vector with C-terminal 6xMyc tag

    Depositor

    Oskar Laur

    Type

    Empty backbone

    Use

    Tags

    6xMyc tag

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Nov. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMT692 ORF2p-3C-3xF (1-1275, ORFeus-Hs) in pDARMO-PolH2.1

    Plasmid

    #213025

    Purpose

    Expresses full length human LINE-1 ORF2p Core (1-1275) with a C-terminal 3xFlag tag for baculovirus production in insect cells

    Depositor

    Martin S. Taylor

    Article

    Baldwin et al Nature. 2024 Feb;626(7997):194-206. doi:

    Insert

    LINE-1 ORF2p (1-1275)
    

    Use

    Tags

    3C-3xFlag (ORF2p)

    Expression

    Insect

    Mutation

    Promoter

    Polyhedrin

    Available since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pWALIUM10-GW-TurboID-V5

    Plasmid

    #196283

    Purpose

    Gateway destination vector to express C-terminal TurboID fusion under UAS promoter, for making transgenic flies

    Depositor

    Norbert Perrimon

    Type

    Empty backbone

    Use

    Gateway destination vector

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    March 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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