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PCDNA3 Flag p38 alpha (agf)

Plasmid

#20352

Depositor

Roger Davis

Article

Enslen et al J Biol Chem. 1998 Jan 16. 273(3):1741-8.

Insert

pDCNA3 Flag p38 alpha (agf) (Mapk14 Mouse)

Tags

Flag

Expression

Mammalian

Mutation

Replace Thr 180 and Tyr 182 with Ala and Phe resp…

Available Since

April 14, 2009

Availability

Academic Institutions and Nonprofits only

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YFP-FKBP (YF)

Plasmid

#20175

Depositor

Tobias Meyer

Article

Inoue et al Nat Methods. 2005 Jun . 2(6):415-8.

Insert

YFP-FKBP (YF): (FKBP1A Human)

Expression

Mammalian

Mutation

FKBP was taken out from pC4M-F2E with a flanking …

Available Since

March 24, 2009

Availability

Academic Institutions and Nonprofits only

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GolgiCFP

Plasmid

#14873

Depositor

Alexandra Newton

Article

Gallegos et al J Biol Chem. 2006 Oct 13. 281(41):30947-

Insert

CFP

Tags

eNOS(1-35)

Expression

Mammalian

Available Since

Aug. 9, 2007

Availability

Academic Institutions and Nonprofits only

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pMXs-Sall4

Plasmid

#13361

Depositor

Shinya Yamanaka

Article

Takahashi et al Cell. 2006 Aug 25. 126(4):663-76.

Insert

sal-like 4 (Drosophila) (Sall4 Mouse)

Use

Retroviral

Expression

Mammalian

Mutation

*(see below)

Available Since

Jan. 12, 2007

Availability

Academic Institutions and Nonprofits only

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pMSCV-TAP

Plasmid

#12570

Depositor

David Sabatini

Article

Frias et al Curr Biol. 2006 Sep 19;16(18):1865-70. d

Type

Empty backbone

Use

Retroviral

Tags

TAP

Expression

Mammalian

Available Since

Sept. 1, 2006

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Casp3 C163A-myc

Plasmid

#11814

Depositor

Guy Salvesen

Article

Stennicke et al J Biol Chem. 1997 Oct 10. 272(41):25719-

Insert

Caspase 3 catalytic mutant (CASP3 Human)

Tags

Myc

Expression

Mammalian

Mutation

C163A

Available Since

July 19, 2006

Availability

Academic Institutions and Nonprofits only

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pMKO.1 puro Rb shRNA

Plasmid

#10670

Depositor

William Hahn

Article

Boehm et al Mol Cell Biol. 2005 Aug . 25(15):6464-74

Insert

Rb shRNA (RB1 Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

Jan. 6, 2006

Availability

Academic Institutions and Nonprofits only

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pU6-Sp-pegRNA-RNF2_+5GtoT

Plasmid

#135957

Purpose

S. pyogenes pegRNA for +5 G to T edit at the RNF2 site of human cells using prime editing

Depositor

David Liu

Article

Anzalone et al Nature. 2019 Oct 21. pii: 10.1038/s41586

Insert

RNF2_5GtoT pegRNA

Expression

Mammalian

Mutation

See manuscript

Promoter

U6

Available Since

Jan. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pCCL-CellCycle

Plasmid

#132429

Purpose

Expressing Fucci cell cycle indicator in mammalian cells

Depositor

John M. Greally

Article

Sato et al J Cell Biol. 2019 Jun 3;218(6):1810-1823

Insert

hCdt, hGeminin

Use

Lentiviral

Expression

Mammalian

Available Since

Oct. 18, 2019

Availability

Academic Institutions and Nonprofits only

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Lentiviral-S-tdTomato

Plasmid

#112579

Purpose

Expressing Shuttle-tdTomato, or NLS-NES-tagged tdTomato in a Lentiviral vector

Depositor

Jeffrey Rothstein

Article

Zhang et al Nature. 2015 Sep 3;525(7567):56-61. doi:

Insert

NLS-tdTomato-NES

Use

Lentiviral

Tags

nuclear export sequence and nuclear localization …

Expression

Mammalian

Available Since

July 24, 2018

Availability

Academic Institutions and Nonprofits only

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PPRE-pNL1.3

Plasmid

#84394

Purpose

Secreted Luciferase reporter for PPARgamma activity

Depositor

Severine Degrelle, Thierry Fournier

Article

Degrelle et al PPAR Res. 2017;2017:6139107. doi: 10.115

Insert

PPARG response element (PPARG Human)

Tags

secNluc

Expression

Mammalian

Promoter

PPRE

Available Since

Jan. 23, 2018

Availability

Academic Institutions and Nonprofits only

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pGP-AAV-CAG-FLEX-jGCaMP7s-WPRE

Plasmid

#104495

Purpose

AAV-mediated expression of ultrasensitive protein calcium sensor under the CAG promoter, Cre mediated flip-excision switch

Depositor

Douglas Kim, GENIE Project

Article

Dana et al Nat Methods. 2019 Jul;16(7):649-657. doi

Has Service

AAV PHP.eB, AAV Retrograde, and AAV1

Insert

jGCaMP7s

Use

AAV and Cre/Lox

Tags

T7 epitope, Xpress tag, 6xHis

Expression

Mammalian

Promoter

CAG

Available Since

Dec. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pCAG-D12L

Plasmid

#89161

Purpose

Express vaccinia virus D12L in mammalian cells

Depositor

Takeshi Kobayashi

Article

Kanai et al Proc Natl Acad Sci U S A. 2017 Jan 30. p

Insert

Vaccinia virus D12L

Expression

Mammalian

Promoter

CAG promotor

Available Since

April 18, 2017

Availability

Academic Institutions and Nonprofits only

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pY094

Plasmid

#84743

Purpose

Expresses huAsCpf1-T2A-GFP and crRNA guide

Depositor

Feng Zhang

Article

Zetsche et al Nat Biotechnol. 2017 Jan;35(1):31-34. do

Inserts

huAsCpf1
GFP

Tags

3xHA, NLS, and T2A

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-PKM2

Plasmid

#64698

Purpose

Expression of GFP tagged PKM2 in mammalian cells

Depositor

Axel Ullrich

Article

Stetak et al Cancer Res. 2007 Feb 15;67(4):1602-8.

Insert

PKM2 (PKM Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 7, 2016

Availability

Academic Institutions and Nonprofits only

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pEGFP-Sec16A

Plasmid

#36155

Purpose

Expresses Sec16A as a GFP fusion

Depositor

David Stephens

Article

Watson et al Traffic. 2006 Dec;7(12):1678-87. Epub 20

Insert

Sec16A (SEC16A Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

July 8, 2015

Availability

Academic Institutions and Nonprofits only

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pCDNA4/Full length FVIII

Plasmid

#41036

Depositor

Robert Peters

Article

Peters et al J Thromb Haemost. 2012 Dec 4. doi: 10.11

Insert

FVIII (F8 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 6, 2012

Availability

Academic Institutions and Nonprofits only

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pECE

Plasmid

#26453

Depositor

William Rutter

Article

Ellis et al Cell. 1986 Jun 6;45(5):721-32.

Type

Empty backbone

Expression

Mammalian

Available Since

Nov. 4, 2011

Availability

Academic Institutions and Nonprofits only

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pCBFRE-luc

Plasmid

#26897

Depositor

Nicholas Gaiano

Article

Yu et al Cell Stem Cell. 2008 May 8. 2(5):461-71.

Insert

CBFRE

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Available Since

Dec. 20, 2010

Availability

Academic Institutions and Nonprofits only
cdk1 FRET sensor (2327)

Plasmid

#26064

Depositor

Jonathon Pines

Article

Gavet et al Dev Cell. 2010 Apr 20. 18(4):533-43.

Insert

Cdk1 FRET Sensor

Tags

CFP variant, FLAG, and YFP variant

Expression

Mammalian

Available Since

Aug. 26, 2010

Availability

Academic Institutions and Nonprofits only

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