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79,901 results

Showing: 2741 - 2760 of 79901 results

  3. pBA-mCherry-EGFP-PIM

    Plasmid
    #111758
    Purpose
    Low level expression of mCherry-EGFP-PIM. Can be used for inducible aggregate formation upon AP20187 addition
    Depositor
    Lukas Kapitein
    Article
    Janssen et al Nat Commun. 2018 Oct 12;9(1):4245. doi:
    Insert
    2x FKBP homo-mCherry-EGFP-2x FKBP homo-2x FKBP
    Expression
    Mammalian
    Mutation
    Val24Glu, Tyr80Cys, Ala95Thr mutations in second …
    Promoter
    chicken beta-actin
    Available Since
    Oct. 30, 2018
    Availability
    Academic Institutions and Nonprofits only
    111758-plasmid-map-sequence-id-222504

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  6. TA1-DuET

    Plasmid
    #213382
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    TA1 (TAAR1 Human)
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Promoter
    CMV, T7
    Available Since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213382-plasmid-map-sequence-id-430737

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  10. pLV-EF1A-CD19.28H.28TM.BB.28.3z

    Plasmid
    #200681
    Purpose
    Modular CD28hinge-CD28transmembrane-41BB-CD28-CD3z CAR backbone, For Transient Expression or Lentiviral Production
    Depositor
    Michele Bernasconi
    Article
    Timpanaro et al J Exp Clin Cancer Res. 2023 Nov 4;42(1):
    Insert
    FMC63-CD28HD-CD28TM-41BB-CD28-CD3z-P2A-EGFP (CD28, CD247, TNFRSF9 Human)
    Use
    Lentiviral
    Tags
    MycTag
    Expression
    Mammalian
    Promoter
    EF1a-short
    Available Since
    Nov. 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    200681-plasmid-map-sequence-id-392612

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  13. dCas9-hHDAC3

    Plasmid
    #98591
    Purpose
    3rd generation lenti vector encoding dCas9-hHDAC3 (EF1a-NLS-dCas9(N863)-hHDAC3). Expresses dCas9 fused to human HDAC3.
    Depositor
    Zhaolan Zhou
    Article
    Kwon et al Nat Commun. 2017 May 12;8:15315. doi: 10
    Insert
    dCAS9-HDAC3 (HDAC3 S. pyogenes, Synthetic, Human)
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Mutation
    D10A and N863A in Cas9
    Promoter
    EF1A
    Available Since
    Oct. 3, 2017
    Availability
    Academic Institutions and Nonprofits only
    98591-plasmid-map-sequence-id-339879

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  15. pCC_05 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-dCas9-NLS-VPR-2A-Puro-WPRE

    Plasmid
    #139090
    Purpose
    Expresses human codon-optimized inactive SpCas9 fused to a transcriptional activator VPR in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a barcode downstream of sgRNA cassette.
    Depositor
    Neville Sanjana
    Article
    Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.
    Insert
    dSpCas9-VPR
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Mutation
    D10A, H840A
    Available Since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    139090-plasmid-map-sequence-id-273886

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  18. mCherry-Rab4a-7

    Plasmid
    #55125
    Purpose
    Localization: Ras GTPase, Excitation: 587, Emission: 610
    Depositor
    Michael Davidson
    Insert
    Rab4a (RAB4A Human)
    Tags
    mCherry
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Sept. 17, 2014
    Availability
    Academic Institutions and Nonprofits only
    55125-plasmid-map-sequence-id-129652

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Showing: 2741 - 2760 of 79901 results