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P4-PE

Plasmid

#211375

Purpose

Packaging plasmid for v3b PE-eVLP production

Depositor

David Liu

Publication

An et al Nat Biotechnol. 2024 Jan 8. doi: 10.1038

Insert

P4-PE

Expression

Mammalian

Available since

Jan. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pmScarlet-I3_C1

Plasmid

#189756

Purpose

C1 Cloning vector for mammalian cells incorporating mScarlet-I3 red fluorescent protein

Depositor

Dorus Gadella

Publication

Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

Insert

mScarlet-I3

Expression

Mammalian

Promoter

CMV

Available since

March 21, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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Cas12f-GE ver4.1

Plasmid

#176544

Purpose

Expresses Cas12f-GE in mammalian cells

Depositor

Yong-Sam Kim

Publication

Kim et al Nat Biotechnol. 2021 Sep 2. pii: 10.1038

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Inserts

Cas12f-GE ver4.1
Cas12f-GE ver4.1

Expression

Mammalian

Promoter

U6 and chicken β-actin

Available since

Oct. 19, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pJRH029

Plasmid

#171060

Purpose

Plasmid for expressing Gag fused to spyCas9-3x FLAG-2x SV40 NLS with the SQNYPIVQ HIV-1 cleavage site in between

Depositor

Jennifer Doudna

Publication

Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:

Insert

Gag-spyCas9

Use

CRISPR and Lentiviral

Tags

2x SV40 NLS and 3x FLAG tag

Expression

Mammalian

Promoter

CAG

Available since

June 30, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pAY5 (mAID-EGFP-NLS piggyBac)

Plasmid

#140532

Purpose

mAID-EGFP-NLS piggyBac

Depositor

Masato Kanemaki

Publication

Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

mAID-EGFP-NLS

Expression

Mammalian

Available since

Nov. 12, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCMV R-CEPIA3mt

Plasmid

#140464

Purpose

Red fluorescent indicator for calcium imaging in the mitochondria (low affinity variant)

Depositor

Masamitsu Iino

Publication

Kanemaru et al Sci Rep. 2020 Feb 18;10(1):2835. doi: 10

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

R-CEPIA3mt

Tags

Myc

Expression

Mammalian

Available since

May 5, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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mNeonGreen-Giantin

Plasmid

#98880

Purpose

In vivo visualization of the Golgi apparatus (can be used for colocalization studies)

Depositor

Dorus Gadella

Publication

Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Giantin

Tags

mNeonGreen

Expression

Mammalian

Promoter

CMV

Available since

March 7, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pUCmini-iCAP-PHP.B

Plasmid

#103002

Purpose

non-standard AAV2 rep-AAV-PHP.B cap plasmid with AAV cap expression controlled by a tTA-TRE amplification system

Depositor

Viviana Gradinaru

Publication

Deverman et al Nat Biotechnol. 2016 Feb;34(2):204-9. do

Insert

Synthetic construct isolate AAV-PHP.B VP1 gene

Use

AAV

Expression

Mammalian

Promoter

p41

Available since

Feb. 7, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

13 Articles

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pHIV-iRFP720-E2A-Luc

Plasmid

#104587

Purpose

Lentivirus vector for bi-cistronic expression of iRFP720 and firefly luciferase from EF1a promoter

Depositor

Antonius Plagge

Publication

Comenge et al Elife. 2018 Jun 27;7. pii: 33140. doi: 1

Insert

iRFP720-E2A-Luciferase

Use

Lentiviral and Luciferase

Tags

bi-cistronic

Expression

Mammalian

Available since

Jan. 9, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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mCherry-Sec61b-C1

Plasmid

#90994

Purpose

Expresses mCherry-tagged Sec61b, brightly labels endoplasmic reticulum in mammalian cells

Depositor

Jennifer Lippincott-Schwartz

Publication

Nixon-Abell et al Science. 2016 Oct 28;354(6311). pii: aaf

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

mCherry-Sec61b (SEC61B Human, Synthetic)

Tags

mCherry (red fluorescence)

Expression

Mammalian

Promoter

CMV

Available since

May 30, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

11 Articles

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pAAV CD68-hM4D(Gi)-mCherry

Plasmid

#75033

Purpose

Expression of hM4D(Gi) using the CD68 promoter.

Depositor

Bryan Roth

Publication

Grace et al Proc Natl Acad Sci U S A. 2016 Jun 14;11

Has service

AAV1 and AAV9

Insert

hM4D(Gi)-mCherry

Use

AAV

Expression

Mammalian

Promoter

CD68

Available since

Oct. 6, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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CIBN-CAAX

Plasmid

#79574

Purpose

expression of N-terminal portion of CIB1 with a C-terminal CAAX box from KRras for plasma-membrane targeting

Depositor

Pietro De Camilli, Olof Idevall-Hagren

Publication

Idevall-Hagren et al Proc Natl Acad Sci U S A. 2012 Aug 28;10

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

CIBN (CIB1 Mustard Weed)

Tags

C-terminal CAAX-box

Expression

Mammalian

Mutation

K93A, R94A, K106A, K107A (please see depositor co…

Promoter

CMV

Available since

July 29, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

16 Articles

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pcDNA3 Flag C/EBPa

Plasmid

#66978

Purpose

Expression plasmid for mouse C/EBPa; adds Flag tag to the N-terminus of protein

Depositor

Christopher Vakoc

Publication

Roe et al Mol Cell. 2015 May 12. pii: S1097-2765(1

Insert

C/EBP alpha (Cebpa Mouse)

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available since

July 28, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pRRL-MHCK7-GCaMP6

Plasmid

#65042

Purpose

Lentiviral vector expressing the calcium indicator GCaMP6 driven by the muscle-specific promoter MHCK7

Depositor

Nenad Bursac

Publication

Madden et al eLife. 2015 Jan 9;4:e04885. doi: 10.7554

Insert

GCaMP6

Use

Lentiviral

Tags

6xHis, T7 tag, and Xpress epitope

Expression

Mammalian

Promoter

MHCK7

Available since

June 2, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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phDLX2-N174

Plasmid

#60860

Purpose

2nd generation lentiviral transfer plasmid. Expresses DLX2 under the EF1a promoter

Depositor

Andrew Yoo

Publication

Victor et al Neuron. 2014 Oct 22;84(2):311-23. doi: 1

Insert

DLX2 (DLX2 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available since

Dec. 5, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pmTurquoise2-C1

Plasmid

#60560

Purpose

Expresses the cyan fluorescent protein variant mTurquoise2, can be used to create fusion proteins

Depositor

Dorus Gadella

Publication

Goedhart et al Nat Commun. 2012 Mar 20;3:751. doi: 10.1

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

mTurquoise2

Expression

Mammalian

Promoter

CMV

Available since

Oct. 31, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

11 Articles

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pAAV-Syn-Chronos-GFP

Plasmid

#59170

Purpose

AAV-mediated expression of Chronos-GFP under the Syn promoter

Depositor

Edward Boyden

Publication

Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

Has service

AAV1, AAV5, and AAV Retrograde

Insert

Chronos-GFP

Use

AAV

Tags

GFP

Expression

Mammalian

Promoter

Syn

Available since

Aug. 13, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

25 Articles

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pC1-HyPer-C199S

Plasmid

#42213

Purpose

Peroxide-insensitive version of HyPer to be used as a pH-control. Can be applied as a pH sensor.

Depositor

Vsevolod Belousov

Publication

Belousov et al Nat Methods. 2006 Apr;3(4):281-6.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

HYPER C199S

Expression

Mammalian

Promoter

CMV

Available since

Dec. 6, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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CAG-GFP/cre

Plasmid

#49054

Purpose

Retroviral vector contains CAG promoter , CMV enhancer and a large synthetic intron that used to allow the ubiquitous expression of GFP-fused Cre recombinase

Depositor

Fred Gage

Publication

Tashiro et al Nat Protoc. 2006;1(6):3049-55.

Insert

GFP/Cre

Use

Retroviral

Expression

Mammalian

Promoter

CAG

Available since

Nov. 4, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pMYC-GFP

Plasmid

#42142

Depositor

Olufunmilayo Olopade

Publication

Chen et al BMC Cancer. 2011 Jun 13;11:246. doi: 10.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

c-Myc (MYC Human)

Tags

GFP

Expression

Bacterial and Mammalian

Promoter

CMV

Available since

Oct. 31, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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