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pSpCas9n(BB)-2A-Puro (PX462) V2.0

Plasmid

#62987

Purpose

Cas9n (D10A nickase mutant) from S. pyogenes with 2A-Puro, and cloning backbone for sgRNA (V2.0)

Depositor

Feng Zhang

Article

Ran et al Nat Protoc. 2013 Nov;8(11):2281-308. doi

Insert

hSpCas9n-2A-Puro

Use

CRISPR

Tags

2A-Puro and 3XFLAG

Expression

Mammalian

Mutation

Cas9 D10A nickase mutant

Available Since

March 11, 2015

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-eGFP-SynaptoZip

Plasmid

#122525

Purpose

AAV expression of the synaptic activity-marker SynaptoZip fused to eGFP driven by human Synapsin promoter

Depositor

Antonio Malgaroli

Article

Ferro et al Nat Commun. 2017 Oct 31;8(1):1229. doi:

Insert

eGFP-SynaptoZip (Vamp2 Rat)

Use

AAV

Tags

Myc and eGFP

Expression

Mammalian

Promoter

hSyn

Available Since

March 10, 2021

Availability

Academic Institutions and Nonprofits only

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AAVS1-Puro CAG PSMA nanobody T-CAR

Plasmid

#171962

Purpose

Donor plasmid for PSMA nanobody T-CAR expression in human cells

Depositor

Xiaoping Bao

Article

Harris et al J Immunol Regen Med. 2023 May;20:100074.

Insert

Synthetic anti-PSMA nanobody CAR

Use

CRISPR, Synthetic Biology, and TALEN

Expression

Mammalian

Promoter

CAG

Available Since

May 5, 2023

Availability

Academic Institutions and Nonprofits only

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pCDNA3-human C-MYC-V394D

Plasmid

#74162

Purpose

Mammalian expression of human cMyc VD mutant

Depositor

Martine Roussel

Article

Vo et al Cancer Cell. 2016 Jan 11;29(1):5-16. doi

Insert

cMyc (MYC Human)

Expression

Mammalian

Mutation

V394D

Promoter

CMV

Available Since

April 11, 2016

Availability

Academic Institutions and Nonprofits only

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pUltra-hot-H2B-mTagBFP2

Plasmid

#161792

Purpose

3rd generation lentiviral multicistronic vector for mammalian expression of mCherry, H2B-mTagBFP2, and one gene of interest

Depositor

Jorge Luis Arias-Arias

Insert

H2B-mTagBFP2

Use

Lentiviral; Multicistronic

Tags

mCherry and mTagBFP2

Expression

Mammalian

Mutation

I174A in the mTagBFP2 protein according to PLoS O…

Promoter

UbC

Available Since

Nov. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV/D374Y-hPCSK9

Plasmid

#58379

Purpose

Expresses GoF mutant human PCSK9 to be used for rAAV8-mediated gene transfer, hypercholesterolemia and atherosclerosis

Depositor

Jacob Bentzon

Article

Bjorklund et al Circ Res. 2014 May 23;114(11):1684-9. do

Has Service

AAV8

Insert

proprotein convertase subtilisin/kexin type 9 (PCSK9 Human)

Use

AAV

Expression

Mammalian

Mutation

D374Y

Promoter

HCRApoE/hAAT

Available Since

Aug. 26, 2014

Availability

Academic Institutions and Nonprofits only

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p3xFLAG CMV14 / BRAF-D594N

Plasmid

#131713

Purpose

Mammalian Expression of Flag tagged BRAF

Depositor

Jacques De Grève

Article

Noeparast et al Oncotarget. 2016 Aug 26;8(36):60094-6010

Insert

BRAF (BRAF Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

D594N

Promoter

CMV

Available Since

Nov. 7, 2024

Availability

Academic Institutions and Nonprofits only

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pT3-EF1a-YapS127A

Plasmid

#46049

Depositor

Xin Chen

Article

Tschaharganeh et al Gastroenterology. 2013 Jun;144(7):1530-1

Insert

Yes-kinase associated protein (YAP1 Human)

Tags

Flag tag

Expression

Mammalian

Mutation

S127A

Promoter

EF1a

Available Since

July 2, 2013

Availability

Academic Institutions and Nonprofits only
pCG-Hc∆18-AA

Plasmid

#86559

Purpose

Plasmid encodes blinded measles virus H glycoprotein

Depositor

Jakob Reiser

Article

Ou et al Hum Gene Ther Methods. 2012 Apr;23(2):13

Insert

Measles virus H glycoprotein

Expression

Mammalian

Mutation

Y463A, R515A

Available Since

Feb. 1, 2017

Availability

Academic Institutions and Nonprofits only

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pCIneo-NHA-LacZ

Plasmid

#115363

Purpose

Expression vector to produce NHA-tagged LacZ protein

Depositor

Witold Filipowicz, Ramesh Pillai

Article

Pillai et al RNA. 2004 Oct . 10(10):1518-25.

Insert

lacZ beta-D-galactosidase

Tags

N peptide and HA

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 21, 2018

Availability

Academic Institutions and Nonprofits only

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lentiCRISPR - AAVS1 sgRNA

Plasmid

#70661

Purpose

Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an AAVS1-targeting sgRNA element from U6 promoter. Lentiviral backbone

Depositor

David Sabatini

Article

Wang et al Science. 2015 Oct 15. pii: aac7041.

Insert

sgRNA against AAVS1

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Oct. 29, 2015

Availability

Academic Institutions and Nonprofits only

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ABE8.20-m

Plasmid

#136300

Purpose

expresses ABE8.20-m in mammalian cells

Depositor

Nicole Gaudelli

Article

Gaudelli et al Nat Biotechnol. 2020 Apr 13. pii: 10.103

Insert

ABE8.20-m

Tags

C-terminal BPNLS

Expression

Mammalian

Mutation

D10A in S. pyogenes Cas9, TadA mutations describe…

Available Since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA5-LAP-KNL1-M3

Plasmid

#115896

Purpose

Used for expression of siRNA-resistant KNL1-M3 (modified codons 258 and 259) from the FRT site of FlpIn cells upon addition of doxycyclin.

Depositor

Geert Kops

Article

Vleugel et al J Cell Biol. 2013 Dec 23;203(6):943-55.

Insert

KNL1-M3 (KNL1 Human)

Tags

EYFP

Expression

Mammalian

Mutation

Fragment of KNL1 containing three KNL1 repeat seq…

Available Since

Oct. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA5FRT/TO-Strep/HA-UBE2QL1

Plasmid

#124665

Purpose

Expresses UBE2QL1 with N-terminal Strep-HA tag in mammalian cells

Depositor

Hemmo Meyer

Article

Koerver et al EMBO Rep. 2019 Aug 21:e48014. doi: 10.15

Insert

UBE2QL1 (UBE2QL1 Human)

Tags

2xStrep/HA

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 24, 2019

Availability

Academic Institutions and Nonprofits only

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pDONR221-SLC16A12_STOP

Plasmid

#161124

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC16A12 (SLC16A12 Human)

Expression

Mammalian

Available Since

June 21, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pmCherryC1 VAP-A

Plasmid

#226407

Purpose

Expression of human VAP-A fused to mCherry in mammalian cells

Depositor

Catherine Tomasetto

Article

Di Mattia et al EMBO Rep. 2018 Jul;19(7). pii: embr.2017

Insert

VAP-A (VAPA Human)

Tags

T7-Xpress tag and mCherry

Expression

Mammalian

Available Since

Oct. 4, 2024

Availability

Academic Institutions and Nonprofits only

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GFP-MAP1B

Plasmid

#44396

Depositor

Phillip Gordon-Weeks

Article

Scales et al J Cell Sci. 2009 Jul 15;122(Pt 14):2424-

Insert

MAP1B (Map1b Mouse)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

May 21, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA-K-GECO1

Plasmid

#105865

Purpose

Mammalian cell expression plasmid for red fluorescent Ca2+ indicator K-GECO1

Depositor

Robert Campbell

Article

Shen et al BMC Biol. 2018 Jan 16;16(1):9. doi: 10.1

Insert

K-GECO1

Expression

Mammalian

Available Since

April 6, 2018

Availability

Academic Institutions and Nonprofits only

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TFORF0786

Plasmid

#142217

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

FOXN1 (FOXN1 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

June 22, 2023

Availability

Academic Institutions and Nonprofits only

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pCDNA3 Flag MFF iso5 S155,172A

Plasmid

#74383

Purpose

Flag tagged human S155,172A MFF isoform 5 mutant

Depositor

Reuben Shaw

Article

Toyama et al Science. 2016 Jan 15;351(6270):275-81. d

Insert

Flag-MFF (MFF Human)

Tags

Flag

Expression

Mammalian

Mutation

See 155 Ala, Ser 172 Ala (numbering based on MFF …

Available Since

April 13, 2016

Availability

Academic Institutions and Nonprofits only