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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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81,793 results

Showing: 2981 - 3000 of 81793 results

  9. PGKneolox2DTA.2

    Plasmid
    #13449
    Depositor
    Philippe Soriano
    Insert
    PGKneolox2DTA
    Use
    Cre/Lox
    Expression
    Mammalian
    Available Since
    Nov. 16, 2006
    Availability
    Academic Institutions and Nonprofits only
    13449-plasmid-map-sequence-id-7635

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  13. Flag-APEX2-C Cloning Vector

    Plasmid
    #128146
    Purpose
    To express a protein of interest fused to the C-terminus of Flag-APEX2 for proximity labeling or TEM
    Depositor
    Ken-Ichi Takemaru
    Type
    Empty backbone
    Tags
    Flag-APEX2
    Expression
    Mammalian
    Available Since
    July 2, 2019
    Availability
    Academic Institutions and Nonprofits only
    128146-plasmid-map-sequence-id-248610

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  14. pCAG-enAsCas12a-HF1(E174R/N282A/S542R/K548R)-NLS(nuc)-3xHA (AAS1815)

    Plasmid
    #107942
    Purpose
    Mammalian expression plasmid for human codon optimized enAsCas12a-HF1 (enhanced high-fidelity AsCas12a) encoding E174R/N282A/S542R/K548R substitutions
    Depositor
    Keith Joung, Benjamin Kleinstiver
    Article
    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103
    Insert
    human codon optimized enAsCas12a-HF1 (E174R/N282A/S542R/K548R)
    Tags
    3x HA and NLS (nucleoplasmin)
    Expression
    Mammalian
    Mutation
    E174R, S542R, K548R, and N282A
    Promoter
    CAG
    Available Since
    Feb. 11, 2019
    Availability
    Academic Institutions and Nonprofits only
    107942-plasmid-map-sequence-id-259760

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  15. pIRE4-Azi

    Plasmid
    #105829
    Purpose
    Plasmid encoding for the translational pair that enables efficent incoporation of p-Azido-phenylalanine in response to the amber codon into proteins in mammmalian cells
    Depositor
    Irene Coin
    Article
    Seidel et al Elife. 2017 Aug 3;6. doi: 10.7554/eLife.
    Inserts
    Humanized EAziRS
    4 copies of the U6-BstYam tRNA cassette
    Expression
    Mammalian
    Mutation
    (Y37L, D182S, F183M, L186A, D265R)TyrRS
    Promoter
    PGK and U6
    Available Since
    March 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    105829-plasmid-map-sequence-id-203267

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  20. Talin-TS

    Plasmid
    #83376
    Purpose
    FRET probe for measuring tension on Talin molecule
    Depositor
    Martin Schwartz
    Article
    Kumar et al J Cell Biol. 2016 May 9;213(3):371-83. d
    Insert
    Talin1 (Tln1 Mouse)
    Use
    Retroviral
    Tags
    EGFP-F40-tagRFP (tension sensor module)
    Expression
    Mammalian
    Mutation
    Tension sensor module is inserted between V447 an…
    Promoter
    CMV
    Available Since
    Oct. 26, 2016
    Availability
    Academic Institutions and Nonprofits only
    83376-plasmid-map-sequence-id-158760

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Showing: 2981 - 3000 of 81793 results