78,292 results

1. NPC1-CFP P692S

   Plasmid

   #58457

   Purpose

   expression of NPC1-CFP sterol sensing domain mutant

   Depositor

   Matthew Scott

   Article

   Ko et al Mol Biol Cell. 2001 Mar;12(3):601-14.

   Insert

   NPC1 (Npc1 Mouse)
   

   Tags

   ECFP and His6

   Expression

   Mammalian

   Mutation

   P692S sterol sensing domain mutant

   Promoter

   CMV

   Available Since

   Oct. 15, 2014

   Availability

   Academic Institutions and Nonprofits only

2. pf CAG mc2 IRES blast

   Plasmid

   #206235

   Purpose

   For expressing cloned exons as circular RNA.

   Depositor

   Simon Conn, Brett Stringer

   Article

   Stringer et al bioRxiv. 2023 Nov 22:2023.11.21.568171.

   Type

   Empty backbone

   Use

   Lentiviral and Synthetic Biology

   Expression

   Mammalian

   Available Since

   Sept. 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pT7-EGFP-C1-HsPAN3

   Plasmid

   #37374

   Depositor

   Elisa Izaurralde

   Article

   Braun et al Mol Cell. 2011 Oct 7;44(1):120-33.

   Insert

   HsPAN3 (PAN3 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 13, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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4. pf CAG mc2 IRES puro

   Plasmid

   #206233

   Purpose

   For expressing cloned exons as circular RNA.

   Depositor

   Simon Conn, Brett Stringer

   Article

   Stringer et al bioRxiv. 2023 Nov 22:2023.11.21.568171.

   Type

   Empty backbone

   Use

   Lentiviral and Synthetic Biology

   Expression

   Mammalian

   Available Since

   Sept. 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. MERTK gRNA (BRDN0001145505)

   Plasmid

   #76444

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human MERTK

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   MERTK (MERTK Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. mEmerald-CD151-7

   Plasmid

   #54032

   Purpose

   Localization: Tetraspanin/Membrane, Excitation: 487, Emission: 509

   Depositor

   Michael Davidson

   Insert

   CD151 (CD151 Human)
   

   Tags

   mEmerald

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 16, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

7. pD649-HAsp-FLT1-Fc(DAPA)-AviTag-6xHis

   Plasmid

   #156962

   Purpose

   Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.

   Depositor

   Chris Garcia

   Article

   Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

   Insert

   FLT1 (FLT1 Human)
   

   Tags

   Fc(DAPA)-AviTag-6xHis

   Expression

   Mammalian

   Mutation

   Fc region of human IgG contains D265A and P329A m…

   Promoter

   CMV/SP6

   Available Since

   March 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. TFORF1722

   Plasmid

   #144305

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   DMRT3 (DMRT3 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   May 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pX330-NQL005-SOX2-sgRNA

   Plasmid

   #175553

   Purpose

   For transient expression of spCas9-nuclease and a sgRNA targeting the mouse SOX2 locus.

   Depositor

   Elzo de Wit

   Article

   Liu et al Nat Genet. 2021 Jan;53(1):100-109. doi:

   Insert

   spCas9-nuclease and sgRNA against mouse SOX2 STOP Codon
   

   Use

   Mouse Targeting

   Expression

   Mammalian

   Available Since

   Nov. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDONR221_SLC22A3

    Plasmid

    #132024

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

    Depositor

    RESOLUTE Consortium, Giulio Superti-Furga

    Article

    Mocking et al Int J Mol Sci. 2022 Jan 21;23(3):1203. d

    Insert

    SLC22A3 (SLC22A3 Human)
    

    Expression

    Mammalian

    Available Since

    Oct. 25, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. HA-MBP-TEVpCS-ADGRL4 CTF-FLAG

    Plasmid

    #217709

    Purpose

    Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

    Depositor

    Stephen Blacklow

    Article

    Dates et al bioRxiv 2022.07.14.500097

    Insert

    ADGRL4 (ADGRL4 Human)
    

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Deletion of residues 1-406

    Available Since

    April 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. ADRB2-DuET

    Plasmid

    #213181

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    ADRB2 (ADRB2 Human)
    

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Promoter

    CMV, T7

    Available Since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. FUGW-ER-TurboID

    Plasmid

    #200965

    Purpose

    3rd gen lentiviral plasmid for expressing endoplasmic reticulum localized TurboID with EGFP

    Depositor

    Keriann Backus

    Article

    Yan et al Cell Chem Biol. 2023 Jul 20;30(7):811-82

    Insert

    ER-TurboID
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Available Since

    Aug. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. HA-MBP-TEVpCS-ADGRL3 CTF-FLAG

    Plasmid

    #217708

    Purpose

    Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

    Depositor

    Stephen Blacklow

    Article

    Dates et al bioRxiv 2022.07.14.500097

    Insert

    ADGRL3 (ADGRL3 Human)
    

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Deletion of residues 1-841

    Available Since

    April 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pEGFP-C1-AR V7

    Plasmid

    #86856

    Purpose

    Fluorescent human androgen-receptor splice variant 7, lacking the ligand-binding domain (fused to EGFP)

    Depositor

    Michael Mancini, Marco Marcelli

    Article

    Szafran et al Prostate. 2017 Jan;77(1):82-93. doi: 10.

    Insert

    Androgen receptor (AR) (AR Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Alternative splice variant 7 (alteration/deletion…

    Promoter

    CMV

    Available Since

    April 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pD649-HAsp-KIR2DL5B-Fc(DAPA)-AviTag-6xHis

    Plasmid

    #156644

    Purpose

    Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.

    Depositor

    Chris Garcia

    Article

    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

    Insert

    KIR2DL5B (KIR2DL5B Human)
    

    Tags

    Fc(DAPA)-AviTag-6xHis

    Expression

    Mammalian

    Mutation

    Fc region of human IgG contains D265A and P329A m…

    Promoter

    CMV/SP6

    Available Since

    March 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3.1-Hyg-BFP

    Plasmid

    #214363

    Purpose

    Expression of the Blue Fluorescent Protein

    Depositor

    Bertrand Collet

    Article

    Chaumont et al BMC Genomics. 2024 Jun 29;25(1):650. doi

    Insert

    BFP
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLVpuro-CMV-N-mCherry

    Plasmid

    #123221

    Purpose

    N-terminal mCherry tag. Gateway destination vector for lentivirus production and stable transduction in mammalian cells.

    Depositor

    Robin Ketteler

    Article

    Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

    Type

    Empty backbone

    Use

    Lentiviral; Gateway destination

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pF CAG mc2 IRES hygro

    Plasmid

    #206234

    Purpose

    For expressing cloned exons as circular RNA.

    Depositor

    Simon Conn, Brett Stringer

    Article

    Stringer et al bioRxiv. 2023 Nov 22:2023.11.21.568171.

    Type

    Empty backbone

    Use

    Lentiviral and Synthetic Biology

    Expression

    Mammalian

    Available Since

    Sept. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA6-N-3XFLAG-Gp78

    Plasmid

    #123590

    Depositor

    Bruce Beutler

    Article

    Choi et al Science. 2019 May 10;364(6440). pii: 364

    Insert

    autocrine motility factor receptor (Amfr Mouse)
    

    Tags

    FLAG

    Expression

    Mammalian

    Available Since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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