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Lenti_gRNA-Puro

Plasmid

#84752

Purpose

Expresses Cpf1 customizable sgRNA from U6 promoter and puromycin resistance from EF-1a promoter

Depositor

Hyongbum Kim

Publication

Kim et al Nat Methods. 2016 Dec 19. doi: 10.1038/n

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available since

Dec. 21, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

21 Articles

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pLifeAct_mScarlet-i_N1

Plasmid

#85056

Purpose

In vivo visualization of actin cytoskeleton (can be used for colocalization studies)

Depositor

Dorus Gadella

Publication

Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

LifeAct

Expression

Mammalian

Promoter

CMV

Available since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

11 Articles

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pdCas9-DNMT3A-PuroR_v2

Plasmid

#74407

Purpose

Expression of human codon-optimized dCas9-DNMT3A fusion with T2A-PuroR (v2) for targeted DNA methylation in mammalian cells; cloning backbone for sgRNA

Depositor

Vlatka Zoldos

Publication

Vojta et al Nucleic Acids Res. 2016 Mar 11. pii: gkw

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

S. pyogenes dCas9 fused with the catalytic domain of human DNMT3A (amino acids P602-V912) and T2A-PuroR_v2 (DNMT3A Human, Synthetic, S. pyogenes)

Use

CRISPR

Tags

3xFLAG, SV40 NLS, and T2A-PuroR

Expression

Mammalian

Mutation

D10A and H840A in S.pyogenes Cas9

Promoter

CBh

Available since

April 26, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

9 Articles

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pEF6-Acly

Plasmid

#70765

Purpose

Expresses mouse ATP-citrate lyase in mammalian cells

Depositor

Kathryn Wellen

Publication

Lee et al Cell Metab. 2014 Aug 5;20(2):306-19. doi

Insert

ATP Citrate Lyase (Acly Mouse)

Tags

myc-epitope

Expression

Mammalian

Promoter

EF1alpha

Available since

Nov. 18, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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Murine E-cadherin mCherry

Plasmid

#71366

Purpose

mammalian expression of murine E-caderin with mCherry fusion

Depositor

Alpha Yap

Publication

Han et al J Biol Chem. 2014 Mar 14;289(11):7764-75

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

E-cadherin (Cdh1 Mouse)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available since

Nov. 17, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pcDNA3.1(+) ZKSCAN1 MCS Exon Vector

Plasmid

#69901

Purpose

Expression plasmid for expressing circular RNAs of a desired sequence in mammalian cells

Depositor

Jeremy Wilusz

Publication

Kramer et al Genes Dev. 2015 Oct 8.

Insert

ZKSCAN1 (ZKSCAN1 Human)

Expression

Mammalian

Promoter

CMV

Available since

Oct. 21, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

10 Articles

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PX404 Campylobacter jejuni Cas9

Plasmid

#68338

Purpose

Campylobacter jejuni Cas9

Depositor

Feng Zhang

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

CjCas9

Use

CRISPR

Tags

HA-NLS and NLS

Expression

Mammalian

Promoter

CMV

Available since

Sept. 30, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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EGFP-E-Syt2

Plasmid

#66831

Purpose

Mammalian Expression, Bacterial Expression

Depositor

Pietro De Camilli

Publication

Giordano et al Cell. 2013 Jun 20;153(7):1494-509. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

E-Syt2 (ESYT2 Human)

Tags

GFP

Expression

Mammalian

Available since

June 22, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pmIL-6 FL

Plasmid

#61286

Purpose

drives luciferase from Wt mouse IL-6 promoter (full length)

Depositor

Gail Bishop

Publication

Baccam et al J Immunol. 2003 Mar 15;170(6):3099-108.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

IL-6 promoter (Il6 Mouse)

Use

Luciferase

Expression

Mammalian

Promoter

IL-6 full length promoter

Available since

May 4, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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phage ubc nls ha pcp gfp

Plasmid

#64539

Purpose

Used to label reporter mRNAs. Lentiviral expression of the PP7 coat protein fused to EGFP.

Depositor

Jeffrey Chao

Publication

Halstead et al Science. 2015 Mar 20;347(6228):1367-671.

Insert

PCP

Use

Lentiviral

Tags

EGFP, FactorXa site, HA, and NLS

Expression

Mammalian

Promoter

human ubiquitin C promoter

Available since

May 1, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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EF05J

Plasmid

#42941

Depositor

Eline Luning Prak

Publication

Farkash et al Nucleic Acids Res. 2006 Feb 28;34(4):119

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Negative control Functionally Dead Human L1 element (L1RE1 Human)

Tags

EGFP (opposite strand)

Expression

Mammalian

Mutation

ORF1 R261A, R262A

Available since

April 25, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pDEST/hCx43-EGFP-N1

Plasmid

#40907

Depositor

Robin Shaw

Publication

Smyth et al Circ Res. 2012 Mar 30;110(7):978-89. Epu

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Connexin 43 (GJA1 Human)

Tags

EGFP

Expression

Mammalian

Mutation

No stop codon to create c-terminal fusion

Promoter

CMV

Available since

Nov. 21, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pCW57.1-4EBP1_4xAla

Plasmid

#38240

Depositor

David Sabatini

Publication

Thoreen et al Nature. 2012 May 2;485(7396):109-13. doi

Insert

4EBP1 (Eif4ebp1 hybrid gene of rat and human, Rat)

Use

Lentiviral; Doxycycline inducible

Expression

Mammalian

Mutation

T37A, T46A, S65A, T70A (dominant negative)

Promoter

Tet ON

Available since

Nov. 15, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

15 Articles

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EGFP-Tpr

Plasmid

#35024

Depositor

Larry Gerace

Publication

Frosst et al J Cell Biol. 2002 Feb 18;156(4):617-30.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Tpr (TPR Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available since

March 12, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pCMV-PTEN

Plasmid

#28298

Depositor

Maria-Magdalena Georgescu

Publication

Takahashi et al EMBO J. 2006 Feb 22. 25(4):910-20.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

PTEN (PTEN Human)

Tags

FLAG

Expression

Mammalian

Available since

Sept. 14, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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SNX9-pmCherryC1

Plasmid

#27678

Depositor

Christien Merrifield

Publication

Taylor et al PLoS Biol. 2011 Mar . 9(3):e1000604.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

SNX9 (Snx9 Mouse)

Tags

mCherry

Expression

Mammalian

Available since

May 4, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pGFP-Rab8A[T22N]

Plasmid

#24899

Depositor

Maxence Nachury

Publication

Nachury et al Cell. 2007 Jun 15. 129(6):1201-13.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

hRab8A[T22N]

Tags

GFP

Expression

Mammalian

Mutation

Changed Threonine 22 to Asparagine

Available since

June 17, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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Flag-HA-USP16

Plasmid

#22595

Depositor

Wade Harper

Publication

Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

Insert

USP16 (USP16 Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

None

Available since

Dec. 16, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pcDNA3 CDK7 HA (P#633)

Plasmid

#14647

Depositor

Matija Peterlin

Publication

Kanazawa et al Oncogene. 2003 Aug 28. 22(36):5707-11.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Cdk7 (CDK7 Human)

Tags

HA

Expression

Mammalian

Available since

June 30, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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EF.GFP

Plasmid

#17616

Depositor

Linzhao Cheng

Publication

Cui et al Blood. 2002 Jan 15. 99(2):399-408.

Insert

EF-1a promoter (EEF1A1 Human)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Available since

April 3, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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