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FUW-hSNCA-GFP

Plasmid

#215489

Purpose

Lentiviral expression and easy visualization of alpha-synuclein in mammalian cells

Depositor

Ophir Shalem

Article

Santhosh Kumar et al Sci Adv. 2024 Feb 9;10(6):eadj4767. doi:

Insert

alpha-synuclein (SNCA Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Promoter

Ubc

Available Since

April 9, 2024

Availability

Academic Institutions and Nonprofits only

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pLEX-FLAG-NRAS-IRES-Puro

Plasmid

#120574

Purpose

Expresses Flag-N-Ras wildtype fusion protein in mammalian cells & for virus production

Depositor

Paul Khavari

Article

Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

Insert

NRAS (NRAS Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 29, 2019

Availability

Academic Institutions and Nonprofits only

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pLX304_CMV::mCherry-LgBiT-FLAG-NES

Plasmid

#199715

Purpose

Expresses LgBiT, fused to mCherry, in the cytosol of mammalian cells. Can be used in generating stable cell lines via lentiviral transduction.

Depositor

Alice Ting

Article

Roh et al J Am Chem Soc. 2023 May 10;145(18):10220

Insert

mCherry-LgBiT-FLAG-NES

Use

Lentiviral and Luciferase

Expression

Mammalian

Promoter

CMV

Available Since

May 9, 2023

Availability

Academic Institutions and Nonprofits only

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pST1374-N-NLS-flag-linker-Cas9-H840A

Plasmid

#51129

Purpose

Cas9 nickase optimized for nuclear import.

Depositor

Xingxu Huang

Article

Shen et al Nat Methods. 2014 Mar 2. doi: 10.1038/nm

Insert

Cas9 nickase

Use

CRISPR

Tags

Flag and NLS

Expression

Mammalian

Mutation

H840A

Promoter

CMV

Available Since

March 7, 2014

Availability

Academic Institutions and Nonprofits only

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pCAGGS-FLAG-mISG15

Plasmid

#12443

Depositor

Dong-Er Zhang

Article

Kim et al Mol Cell Biol. 2004 Nov . 24(21):9592-60

Insert

ISG15

Tags

FLAG

Expression

Mammalian

Available Since

Jan. 4, 2007

Availability

Academic Institutions and Nonprofits only

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p6-8

Plasmid

#118089

Purpose

rapa-T7- mRNA(GFP)

Depositor

Bryan Dickinson

Article

Pu et al Nat Chem Biol. 2017 Apr;13(4):432-438. d

Insert

Rapamycin-inducible split T7 RNAP with T7 driven mRNA for GFP

Expression

Mammalian

Available Since

Nov. 7, 2018

Availability

Academic Institutions and Nonprofits only

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BRD4-N CRISPR

Plasmid

#140651

Purpose

BRD4 tagging CRISPR

Depositor

Masato Kanemaki

Article

Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

Insert

BRD4 (BRD4 Human)

Use

CRISPR

Expression

Mammalian

Available Since

Nov. 12, 2020

Availability

Academic Institutions and Nonprofits only

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LentiV_Cas9_puro

Plasmid

#108100

Purpose

Lentiviral expression plasmid of spCas9 with puromycin resistance gene

Depositor

Christopher Vakoc

Article

Tarumoto et al Mol Cell. 2018 Feb 28. pii: S1097-2765(1

Insert

Cas9

Use

CRISPR and Lentiviral

Tags

FLAG

Expression

Mammalian

Promoter

EFS promoter

Available Since

July 5, 2018

Availability

Academic Institutions and Nonprofits only

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GFP-HP1gamma

Plasmid

#17650

Depositor

Tom Misteli

Article

Cheutin et al Science. 2003 Jan 31. 299(5607):721-5.

Insert

HP1 gamma (CBX3 Human)

Tags

GFP and His

Expression

Mammalian

Available Since

March 27, 2008

Availability

Academic Institutions and Nonprofits only

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pCMV-lyso-pHoenix

Plasmid

#70112

Purpose

Expression of lyso-pHoenix in mammalian cells for optogenetic acidification of lysosomes

Depositor

Christian Rosenmund

Article

Rost et al Nat Neurosci. 2015 Nov 9. doi: 10.1038/n

Insert

lyso-pHoenix (Cd63 Rat, Halorubrum sodomense, Entacmaea quadricolor, Aequorea victoria, Mouse)

Tags

Arch3, H+/K+ ATPase beta-subunit, HA-Tag, mKate2,…

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 11, 2015

Availability

Academic Institutions and Nonprofits only

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pDualEX-SMN2

Plasmid

#214431

Purpose

Dual-color reporter express eGFP constitutively; tdTomato expression is contingent on the exclusion of the frame-shifting cassette exon 7

Depositor

Chaolin Zhang

Article

Recinos et al Nat Commun. 2024 May 7;15(1):3839. doi:

Insert

SMN2 EXON 7 (SMN2 Human)

Expression

Mammalian

Available Since

May 13, 2024

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPR-EGFP

Plasmid

#75159

Purpose

Derived from LentiCRISPR v1 but expresses EGFP instead of puromycin resistance

Depositor

Beat Bornhauser

Article

McComb et al Sci Transl Med. 2016 May 18;8(339):339ra

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

EGFP

Expression

Mammalian

Available Since

May 17, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3-RAGE

Plasmid

#71435

Purpose

mammalian expression of human RAGE

Depositor

Henri Huttunen

Article

Huttunen et al J Biol Chem. 1999 Jul 9;274(28):19919-24

Insert

RAGE (AGER Human)

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 25, 2015

Availability

Academic Institutions and Nonprofits only

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pCI-neo-sOVA

Plasmid

#25098

Depositor

Maria Castro

Article

Yang et al Proc Natl Acad Sci U S A. 2010 Mar 9. 10

Insert

soluble ovalbumin

Expression

Bacterial and Mammalian

Available Since

July 1, 2010

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Casp8

Plasmid

#11817

Depositor

Guy Salvesen

Article

Stennicke et al J Biol Chem. 1997 Oct 10. 272(41):25719-

Insert

Caspase 8 (CASP8 Human)

Expression

Mammalian

Available Since

May 25, 2006

Availability

Academic Institutions and Nonprofits only

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mRaspberry-N1

Plasmid

#54645

Purpose

Localization: N1 Cloning Vector, Excitation: 598, Emission: 625

Depositor

Michael Davidson

Article

Kremers et al Nat Methods. 2009 May;6(5):355-8. doi: 1

Type

Empty backbone

Tags

mRaspberry

Expression

Mammalian

Available Since

June 12, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 TEV (full-length)

Plasmid

#64276

Purpose

Expresses tobacco etch virus protease (TEV)

Depositor

Xiaokun Shu

Article

To et al Proc Natl Acad Sci U S A. 2015 Mar 17;11

Insert

Tobacco etch virus (TEV) protease

Expression

Mammalian

Promoter

CMV

Available Since

April 24, 2015

Availability

Academic Institutions and Nonprofits only

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pCAG-ETF1-E55D

Plasmid

#130876

Purpose

Encodes ETF1 with an E55D substitution

Depositor

Prashant Mali

Article

Katrekar et al Sci Rep. 2018 Feb 26;8(1):3589. doi: 10.

Insert

Eukaryotic transcritional termination factor ETF1-E55D (ETF1 Human)

Expression

Mammalian

Mutation

E55D

Promoter

CAG

Available Since

Sept. 3, 2019

Availability

Academic Institutions and Nonprofits only

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AAV_pMBP-EGFP-caax

Plasmid

#190155

Purpose

Expresses membrane-targeted EGFP in oligodendrocytes; AAV vector

Depositor

Brad Zuchero

Article

Lam et al Nat Commun. 2022 Sep 23;13(1):5583. doi:

Has Service

AAV1 and AAV8

Insert

EGFP-caax

Use

AAV

Expression

Mammalian

Promoter

MBP

Available Since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pLV-EF1A-CD19.8H.28TM.28.BB.3z

Plasmid

#200676

Purpose

Modular CD8hinge-CD28transmembrane-CD28-41BB-CD3z CAR backbone, For Transient Expression or Lentiviral Production

Depositor

Michele Bernasconi

Article

Timpanaro et al J Exp Clin Cancer Res. 2023 Nov 4;42(1):

Insert

FMC63-CD8HD-CD28TM-CD28-41BB-CD3z-P2A-EGFP (CD8A, CD28, CD247, TNFRSF9 Human)

Use

Lentiviral

Tags

MycTag

Expression

Mammalian

Promoter

EF1a-short

Available Since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

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