78,292 results

1. pEM705 Gso (Ser)

   Plasmid

   #109353

   Purpose

   GsX chimera with Gαo C-terminus (Pertussis toxin insensitive mutant)

   Depositor

   Robert Lucas

   Article

   Ballister et al BMC Biol. 2018 Jan 16;16(1):10. doi: 10.

   Insert

   Gso chimera
   

   Tags

   None

   Expression

   Mammalian

   Mutation

   Changed Cysteine at position 391 to Serine (Pertu…

   Promoter

   CAG

   Available Since

   May 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. Flag-HA-UCHL1

   Plasmid

   #22563

   Depositor

   Wade Harper

   Article

   Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

   Insert

   UCHL1 (UCHL1 Human)
   

   Use

   Retroviral

   Tags

   FLAG and HA

   Expression

   Mammalian

   Mutation

   None

   Available Since

   Nov. 16, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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3. myc-BioID2-MCS

   Plasmid

   #74223

   Purpose

   To fuse your protein of interest to the C-terminus of BioID2 and use in proximity-dependent biotin identification (BioID); MCS is present downstream of myc tag and BioID2 tag

   Depositor

   Kyle Roux

   Article

   Kim et al Mol Biol Cell. 2016 Feb 24. pii: mbc.E15

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Tags

   Myc and humanized A. aeolicus BioID2 with R40G

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMSCV-F-del Casp9.IRES.GFP

   Plasmid

   #15567

   Depositor

   David Spencer

   Article

   Straathof et al Blood. 2005 Jun 1. 105(11):4247-54.

   Insert

   FKBP12(V36)-p30Caspase9 (FKBP1A Human)
   

   Use

   Retroviral

   Tags

   FKBP12 and HA epitope

   Expression

   Mammalian

   Mutation

   FKBP12 F36 to V Truncated Caspase9 (135-416; lac…

   Available Since

   Aug. 22, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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5. lenti sgRNA(MS2)_puro backbone

   Plasmid

   #73795

   Purpose

   3rd generation lenti sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2 and EF1a-puro resistance marker. Contains BsmBI sites for insertion of spacer sequences.

   Depositor

   Feng Zhang

   Article

   Konermann et al Nature. 2014 Dec 10. doi: 10.1038/nature

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6 and EF1A

   Available Since

   March 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1-Beta3

   Plasmid

   #140988

   Purpose

   Encodes a Gbeta subunit (GNB3) as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

   Depositor

   Bryan Roth

   Article

   Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

   Insert

   GBeta3 (GNB3 Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. MGAT1-GFP

   Plasmid

   #163647

   Purpose

   Expresses GFP tagged MGAT1 in mammalian cells

   Depositor

   Lei Lu

   Article

   Sun et al J Cell Sci. 2021 Oct 15;134(20). pii: 27

   Insert

   alpha-1,3-mannosyl-glycoprotein beta-1, 2-N-acetylglucosaminyltransferase (MGAT1 Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Available Since

   Nov. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. Integrin β5-GFP

   Plasmid

   #205090

   Purpose

   Express GFP-tagged human integrin β5 in mammalian cells

   Depositor

   Bianxiao Cui

   Article

   Zhang et al Nat Cell Biol. 2023 Sep 28. doi: 10.1038

   Insert

   Integrin beta 5 (ITGB5 Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Available Since

   Sept. 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-U6-sasgRNACMV- mCherry-WPREpA

   Plasmid

   #217015

   Purpose

   S. aureus sgRNAs backbone along with mCherry expression

   Depositor

   Nadav Ahituv

   Article

   Matharu et al Science. 2018 Dec 13. pii: science.aau06

   Insert

   mCherry
   

   Use

   AAV and CRISPR

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. ph7SK-gRNA

    Plasmid

    #53189

    Purpose

    Expresses the S. pyogenes sgRNA from the 7SK promoter

    Depositor

    Charles Gersbach

    Article

    Kabadi et al Nucleic Acids Res. 2014 Aug 13. pii: gku

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    human 7SK

    Available Since

    June 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. VPS13A^Halo

    Plasmid

    #118759

    Purpose

    VPS13A internally tagged with Halo at residue 1372.

    Depositor

    Pietro De Camilli

    Article

    Kumar et al J Cell Biol. 2018 Oct 1;217(10):3625-363

    Insert

    VPS13A (VPS13A Human)
    

    Tags

    Halo tag inserted at position 1372

    Expression

    Mammalian

    Available Since

    Jan. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.1/opto-a1AR-EYFP

    Plasmid

    #20947

    Purpose

    Mammalian expression of opto-a1AR fused to EYFP for optical control of GPCR signaling

    Depositor

    Karl Deisseroth

    Article

    Airan et al Nature. 2009 Apr 23;458(7241):1025-9. do

    Insert

    opto-a1AR
    

    Expression

    Mammalian

    Available Since

    May 28, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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13. pB-rtTA

    Plasmid

    #126034

    Purpose

    PiggyBac cargo vector expressing rtTA. Also confers resistance to G418.

    Depositor

    Mauro Calabrese

    Article

    Kirk et al Nat Genet. 2018 Oct;50(10):1474-1482. do

    Insert

    rtTA3-IRES-Neo cassette cloned from Addgene Plasmid #25735
    

    Use

    Piggybac

    Expression

    Mammalian

    Promoter

    hUbiC

    Available Since

    June 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLenti6/V5/DEST-VPS35

    Plasmid

    #21691

    Depositor

    Lynda Chin

    Article

    Scott et al Nature. 2009 Jun 25. 459(7250):1085-90.

    Insert

    Vacuolar Protein Sorting 35 homolog (VPS35 Human)
    

    Use

    Lentiviral; Invitrogen gateway destination vector

    Tags

    V5

    Expression

    Mammalian

    Available Since

    July 28, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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15. Man2a1-GFP

    Plasmid

    #163649

    Purpose

    Expresses GFP tagged Man2a1 in mammalian cells

    Depositor

    Lei Lu

    Article

    Sun et al J Cell Sci. 2021 Oct 15;134(20). pii: 27

    Insert

    mannosidase alpha class 2A member 1 (MAN2A1 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Silent mutation was made to introduce a XbaI rest…

    Available Since

    Nov. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pPAmCherry2-C1

    Plasmid

    #31932

    Depositor

    Vladislav Verkhusha

    Article

    Subach et al Nat Methods. 2009 Feb;6(2):153-9. Epub 2

    Insert

    PAmCherry2
    

    Expression

    Mammalian

    Mutation

    M18L/K69N/L84F/A147T/S148L/I165L/Q167A/L169T/A179…

    Promoter

    CMV

    Available Since

    Sept. 12, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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17. Tet1-dCas9

    Plasmid

    #136650

    Purpose

    Tet1 fused to N-terminus of dCas9; pCDNA3 vector backbone, mammalian expression

    Depositor

    David Segal

    Article

    Umeh-Garcia et al Br J Cancer. 2022 Apr 19. pii: 10.1038/s

    Insert

    Tet1 (Tet1 Mouse)
    

    Use

    CRISPR

    Tags

    3XFLag-NLS-Tet1-dCas9-NLS

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. Lamp1-RFP

    Plasmid

    #1817

    Purpose

    Mammalian expression of Lamp1 fused to RFP. Used for localization to the lysosome.

    Depositor

    Walther Mothes

    Article

    Sherer et al Traffic 2003 Nov;4(11):785-801.

    Insert

    lysosome associated membrane protein 1 (Lamp1 Rat)
    

    Tags

    RFP

    Expression

    Mammalian

    Mutation

    D50E (not important for function of plasmid)

    Available Since

    Sept. 26, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBR_attB(bxb)_lox

    Plasmid

    #183762

    Purpose

    Bxb1-specific donor plasmid for the cloning of payloads <20 kb

    Depositor

    Richard Davis

    Article

    Blanch-Asensio et al Cell Rep Methods. 2022 Sep 22;2(10):1003

    Type

    Empty backbone

    Use

    Cre/Lox and Synthetic Biology

    Expression

    Mammalian

    Available Since

    Sept. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pEF6-Acly(S455D)

    Plasmid

    #70768

    Purpose

    Expresses phosphomimetic mouse ATP-citrate lyase in mammalian cells

    Depositor

    Kathryn Wellen

    Article

    Lee et al Cell Metab. 2014 Aug 5;20(2):306-19. doi

    Insert

    ATP Citrate Lyase (Acly Mouse)
    

    Tags

    myc-epitope

    Expression

    Mammalian

    Mutation

    S455D

    Promoter

    EF1alpha

    Available Since

    Nov. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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