79,207 results

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MSCV-N E4orf1

Plasmid

#38063

Depositor

Karl Munger

Article

Rozenblatt-Rosen et al Nature. 2012 Jul 26;487(7408):491-5.

Insert

E4 orf1

Use

Retroviral

Tags

Flag and HA

Expression

Mammalian

Promoter

PKG

Available Since

Aug. 3, 2012

Availability

Academic Institutions and Nonprofits only

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pAAV-Neo_CAG-Cas9

Plasmid

#86698

Purpose

Cas9 expression vector for targeting to the AAVS1 locus

Depositor

Ludovic Vallier

Article

Bertero et al Development. 2016 Dec 1;143(23):4405-441

Insert

Cas9

Use

CRISPR

Expression

Mammalian

Promoter

pCag

Available Since

Jan. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pLX304 CMV FKBP-V5-sTurboID-high affinity (N)

Plasmid

#153004

Purpose

Expresses high-affinity split-TurboID N-terminal fragment in mammalian cells

Depositor

Alice Ting

Article

Cho et al Proc Natl Acad Sci U S A. 2020 May 18. p

Insert

sTurboID-high affinity (N) (G78)

Use

Lentiviral

Tags

FKBP and V5

Expression

Mammalian

Promoter

CMV

Available Since

June 16, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/Myc-His(-)-HSulf-2

Plasmid

#13004

Depositor

Steven Rosen

Article

Morimoto-Tomita et al J Biol Chem. 2002 Dec 20. 277(51):49175-

Insert

human sulfatase 2 (SULF2 Human)

Tags

6x His and Myc

Expression

Mammalian

Available Since

Oct. 30, 2006

Availability

Academic Institutions and Nonprofits only

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scFv-GCN4-DNMT3a(R887E)-DNMT3l

Plasmid

#154141

Purpose

Expresses a higher specificity variant of scFv-GCN4-DNMT3a-DNMT3l, contains R887E mutation in DNMT3a. To be used with the dCas9-SunTag system for targeted DNA methylation.

Depositor

Albert Jeltsch

Article

Hofacker et al Int J Mol Sci. 2020 Jan 13;21(2). pii: i

Insert

scFv-GCN4, DNMT3a (catalytic domain), DNMT3l (C-terminal part), sfGFP

Tags

HA and sfGFP

Expression

Mammalian

Mutation

R887E

Promoter

SFFV

Available Since

Oct. 19, 2021

Availability

Academic Institutions and Nonprofits only

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HA GSK3 beta S9A pcDNA3

Plasmid

#14754

Purpose

Mammalian expression of human GSK3 beta constitutively active mutant with HA tag

Depositor

Jim Woodgett

Article

Stambolic et al Biochem J. 1994 Nov 1;303 ( Pt 3)(Pt 3):

Insert

GSK3 beta S9A (GSK3B Human)

Tags

HA

Expression

Mammalian

Mutation

S9A. Constitutively active mutant.

Available Since

April 12, 2007

Availability

Industry, Academic Institutions, and Nonprofits

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FLAG-PRMT6

Plasmid

#164697

Purpose

Expression of PRMT6 with an N-terminal FLAG tag

Depositor

Cheryl Arrowsmith

Article

Eram et al ACS Chem Biol. 2016 Mar 18;11(3):772-781

Insert

PRMT6 (PRMT6 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

March 10, 2021

Availability

Academic Institutions and Nonprofits only

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pInducer20 Cyclin E1

Plasmid

#109348

Purpose

virus packaged, doxycyline inducible expression of Cyclin E1

Depositor

Jean Cook

Article

Matson et al Elife. 2017 Nov 17;6. doi: 10.7554/eLife

Insert

Cyclin E1 (CCNE1 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

July 25, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-ROR2-V5/HIS

Plasmid

#205608

Purpose

Expression of human ROR2 receptor tyrosine kinase in mammalian cells

Depositor

Pavel Krejčí

Article

Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

Insert

ROR2 receptor tyrosine kinase (ROR2 Human)

Tags

V5-His

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pmRFP-N1 human cofilin WT

Plasmid

#50856

Purpose

expresses human cofilin fused to mRFP in mammalian cells

Depositor

James Bamburg

Article

Garvalov et al J Neurosci. 2007 Nov 28;27(48):13117-29.

Insert

cofilin 1 (CFL1 Human)

Tags

mRFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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YFP-Parkin

Plasmid

#23955

Purpose

Mammalian expression of human Park2 fused to EYFP

Depositor

Richard Youle

Article

Narendra et al J Cell Biol. 2008 Dec 1. 183(5):795-803.

Insert

Park2 (PRKN Human)

Tags

eYFP

Expression

Mammalian

Available Since

April 9, 2010

Availability

Academic Institutions and Nonprofits only

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pFlagCMV2-EFP

Plasmid

#12449

Depositor

Dong-Er Zhang

Article

Zou et al J Biol Chem. 2006 Feb 17. 281(7):3989-94

Insert

EFP (TRIM25 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 4, 2007

Availability

Academic Institutions and Nonprofits only

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tdTomato-Integrin-Beta1-N-18

Plasmid

#58105

Purpose

Localization: Integrin/Focal Adhesions, Excitation: 554, Emission: 581

Depositor

Michael Davidson

Insert

Integrin-Beta1 (ITGB1 Human)

Tags

tdTomato

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 5, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 His-GFP-EWSR1-myc-His

Plasmid

#46385

Purpose

expresses EGFP tagged EWSR1 in mammalian cells

Depositor

Heinz Gehring

Article

Pahlich et al Proteins. 2008 Sep;72(4):1125-37. doi: 1

Insert

EWSR1 (EWSR1 Human)

Tags

EGFP, His, and myc

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 5, 2013

Availability

Academic Institutions and Nonprofits only
GFP-Ub

Plasmid

#11928

Purpose

Used for in vivo monitoring of ubiquitin localization.

Depositor

Nico Dantuma

Article

Dantuma et al J Cell Biol. 2006 Apr 10. 173(1):19-26.

Insert

Ubiquitin (UBC Human)

Tags

GFP

Expression

Mammalian

Available Since

June 22, 2006

Availability

Academic Institutions and Nonprofits only

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pLV x EF1a_EKAREN4-ires-puro

Plasmid

#167828

Purpose

Optimized EKAREV FRET biosensor sensor for ERK

Depositor

Hugo Snippert

Article

Ponsioen et al Nat Cell Biol. 2021 Apr;23(4):377-390. d

Insert

EKAREN4

Use

Lentiviral

Tags

nls localization motif

Expression

Mammalian

Mutation

K424P, K426W

Promoter

EF1a

Available Since

April 20, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV-Luc

Plasmid

#45968

Depositor

Ralf Kuehn

Insert

Luciferase

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 14, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)Gαi1-91-NLuc

Plasmid

#207148

Purpose

Part of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes GNAI1 tagged with NanoLuciferase and is meant to be paired with Ggamma2 labeled with LSS-mKATE2

Depositor

Dominique Schols, Tom Van Loy

Article

Boon et al Biosensors (Basel). 2023 Jul 28;13(8):76

Insert

GNAI1 (GNAI1 Human)

Expression

Mammalian

Mutation

NLuc inserted at position L(91)K

Available Since

Nov. 20, 2023

Availability

Academic Institutions and Nonprofits only

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Flag-FOXP3

Plasmid

#153147

Purpose

Expression of Flag tagged human FOXP3 in mammalian cells

Depositor

Stefan Koch

Article

Moparthi et al Differentiation. 2020 Sep - Oct;115:30-3

Insert

human FOXP3 (FOXP3 Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV IE94

Available Since

Aug. 24, 2020

Availability

Academic Institutions and Nonprofits only

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pCEP4-HA-BAX

Plasmid

#16587

Depositor

Bert Vogelstein

Article

Yu et al Mol Cell. 2001 Mar . 7(3):673-82.

Insert

BAX alpha (BAX Human)

Tags

2x HA

Expression

Mammalian

Available Since

March 10, 2008

Availability

Academic Institutions and Nonprofits only

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