77,958 results

1. -5.5kb-UCP1-GFP

   Plasmid

   #104585

   Purpose

   Reporter assay to monitor the transcription activity of the UCP1 promoter by GFP expression

   Depositor

   Irfan Lodhi

   Article

   Lodhi et al Cell Rep. 2017 Sep 19;20(12):2766-2774.

   Insert

   UCP1 promoter (Ucp1 Mouse)
   

   Tags

   TurboGFP

   Expression

   Mammalian

   Promoter

   UCP1

   Available Since

   Dec. 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. ADSL_pLX307

   Plasmid

   #98311

   Purpose

   Lentiviral expression of ADSL

   Depositor

   William Hahn, Sefi Rosenbluh

   Article

   Rosenbluh et al Cell Syst. 2016 Sep 28;3(3):302-316.e4.

   Insert

   ADSL (ADSL Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Promoter

   E1Fa

   Available Since

   Aug. 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pHFS:SEP-rGluA2 TKIT donor

   Plasmid

   #169440

   Purpose

   Donor DNA for TKIT knockin of SEP to GluA2 in rat

   Depositor

   Richard Huganir

   Article

   Fang et al Elife. 2021 Mar 10;10. pii: 65202. doi:

   Insert

   Super ecliptic pHluorin (SEP)
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   June 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. dCas9-hHDAC3

   Plasmid

   #98591

   Purpose

   3rd generation lenti vector encoding dCas9-hHDAC3 (EF1a-NLS-dCas9(N863)-hHDAC3). Expresses dCas9 fused to human HDAC3.

   Depositor

   Zhaolan Zhou

   Article

   Kwon et al Nat Commun. 2017 May 12;8:15315. doi: 10

   Insert

   dCAS9-HDAC3 (HDAC3 Human, Synthetic, S. pyogenes)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Mutation

   D10A and N863A in Cas9

   Promoter

   EF1A

   Available Since

   Oct. 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. MCP-mCherry

   Plasmid

   #207668

   Purpose

   To express fluorescently labeled MS2 coat binding protein (MCP)

   Depositor

   Christine Mayr

   Article

   Horste et al Mol Cell. 2023 Dec 21;83(24):4509-4523.e

   Insert

   MCP-mCherry
   

   Expression

   Mammalian

   Available Since

   Sept. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pPB-Ins-TRE3Gp-dCas9-Suntag-VP64-EF1Ap-Puro

   Plasmid

   #183409

   Purpose

   piggyBAC-based doxycycline-inducible dCas9-5xSuntag-VP64 CRISPR activation plasmid for enhancer and promoter activation in mammalian cells

   Depositor

   Azim Surani

   Article

   Tang et al Nat Cell Biol. 2022 Apr;24(4):448-460. d

   Insert

   dCas9-5xGCN5-P2A-scFV-sfGFP-VP64-GB1
   

   Use

   CRISPR and Synthetic Biology; Piggybac

   Expression

   Mammalian

   Promoter

   TRE3G

   Available Since

   May 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3_QLuc

   Plasmid

   #183260

   Purpose

   Express QLuc in mammalian cells

   Depositor

   Huiwang Ai

   Article

   Xiong et al J Am Chem Soc. 2022 Aug 1. doi: 10.1021/

   Insert

   QLuc
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCSIIbleo-iRFP670-P2A-EGFP

   Plasmid

   #198060

   Purpose

   The lentiviral vector for iRFP670 and EGFP

   Depositor

   Michiyuki Matsuda

   Insert

   iRFP670
   

   Use

   Lentiviral

   Tags

   P2A-EGFP

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Aug. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV.Syn.NES-jRGECO1a.WPRE.SV40

   Plasmid

   #100854

   Purpose

   AAV expression of jRGECO1a, a red fluorescent calcium sensor protein, from the Synapsin promoter

   Depositor

   GENIE Project, Douglas Kim

   Article

   Dana et al eLife. 2016 Mar 24;5. pii: e12727. doi:

   Has Service

   AAV PHP.eB, AAV Retrograde, AAV1, and AAV9

   Insert

   jRGECO1a
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   Synapsin

   Available Since

   Nov. 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. piggyBac-rtTA (4th_Gen)-SNCA(3K)-sfGFP-IRES-NGN2-puro (VK_1120)

    Plasmid

    #209078

    Purpose

    Ngn2 mediated cortical neuron induction, along with SNCA(3K) over-expression

    Depositor

    Vikram Khurana

    Article

    Lam et al bioRxiv 2022.11.08.515615

    Inserts

    SNCA (SNCA Human)
    NGN2 (Neurog2 Mouse)
    

    Tags

    sfGFP

    Expression

    Mammalian

    Mutation

    3K mutant: E35K, E46K, E61K

    Promoter

    TRE4G

    Available Since

    Dec. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. GFP-mROCK2

    Plasmid

    #101296

    Purpose

    Mammalian expression of Rock2-GFP

    Depositor

    Alpha Yap

    Article

    Priya et al Nat Cell Biol. 2015 Oct;17(10):1282-93.

    Insert

    Rock2 (Rock2 Mouse)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCW57.1 blast tBID-flag

    Plasmid

    #211533

    Purpose

    Expresses truncated BID upon doxycycline addition to trigger apoptosis

    Depositor

    Richard Possemato

    Article

    Nam et al Nat Metab. 2024 Dec;6(12):2338-2353. doi

    Insert

    tBID-flag (BID Human)
    

    Use

    Retroviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Truncated (CASP8) form (p15)

    Available Since

    Jan. 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. PB-sgRNA(MS2)

    Plasmid

    #102560

    Purpose

    Piggybac transposon sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2. Contains BbsI sites for insertion of spacer sequences.

    Depositor

    Ying Liu

    Article

    Li et al Mol Ther Nucleic Acids. 2017 Sep 15;8:64

    Type

    Empty backbone

    Use

    CRISPR; Piggybac transposon

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Jan. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAdEasy-2

    Plasmid

    #16401

    Purpose

    Adenoviral vector for use in AdEasy System, for use with large inserts.

    Depositor

    Bert Vogelstein

    Article

    He et al Proc Natl Acad Sci U S A. 1998 Mar 3. 95

    Type

    Empty backbone

    Use

    Adenoviral

    Expression

    Mammalian

    Available Since

    June 11, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDsRed-Sensor

    Plasmid

    #22743

    Depositor

    Lynn Hudson

    Article

    Lau et al J Neurosci. 2008 Nov 5. 28(45):11720-30.

    Insert

    modified pCMV-DsRed-Express (Clontech)
    

    Expression

    Mammalian

    Available Since

    Dec. 18, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLenti-H3.3(WT)-3AID-HA-2A-mCherryBSD

    Plasmid

    #225697

    Purpose

    Lentiviral expression of AID degron tagged Wildtype H3.3 and mCherry fused BlasticidinR

    Depositor

    Lacra Bintu

    Article

    Sinha et al bioRxiv [Preprint]. 2023 Oct 14:2023.10.

    Insert

    H3.3 (WT) (H3-3A, H3-3B Human)
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    3xAID HA and T2A-mCherry

    Expression

    Mammalian

    Available Since

    Oct. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAcGFP1-N1-RvCAHS12-GS

    Plasmid

    #192462

    Purpose

    Expression of RvCAHS12-GS_linker-AcGFP1 in mammalian cells

    Depositor

    Takekazu Kunieda

    Article

    Tanaka et al PLoS Biol. 2022 Sep 6;20(9):e3001780. do

    Insert

    CAHS12
    

    Tags

    AcGFP1

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. canxTM-mEmerald (cytosolic)

    Plasmid

    #186962

    Purpose

    Expresses mEmerald connected with the C-terminus of Calnexin transmembrane domain.

    Depositor

    Ke Xu

    Article

    Wang et al Proc Natl Acad Sci U S A. 2022 May 3;119

    Insert

    CANX (CANX Human)
    

    Tags

    mEmerald

    Expression

    Mammalian

    Mutation

    Only express calnexin (482-end) and add ER signal…

    Promoter

    CMV Promoter

    Available Since

    Sept. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-hSyn-DIO-FLAG-KORD-P2A-3xHA-hM3D(Gq)

    Plasmid

    #220611

    Purpose

    Neuron-specific, Cre-dependent co-expression of FLAG-tagged inhibitory KORD DREADD receptor, and 3x HA-tagged excitatory hM3D(Gq) DREADD receptor.

    Depositor

    Sam Golden

    Article

    Szelenyi et al Proc Natl Acad Sci U S A. 2024 Aug 6;121

    Insert

    FLAG-KORD-P2A-3x HA-hM3D(Gq)
    

    Use

    AAV and Cre/Lox

    Tags

    FLAG; ArgiNLS

    Expression

    Mammalian

    Promoter

    hSyn

    Available Since

    Aug. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCS2+N-3xHA Cloning Vector

    Plasmid

    #196653

    Purpose

    N-terminal 3xHA mammalian expression plasmid

    Depositor

    Ken-Ichi Takemaru

    Type

    Empty backbone

    Tags

    3xHA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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