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pVenus-N1 (804)

Plasmid

#61854

Purpose

empty backbone for tagging proteins at the C-terminus with Venus

Depositor

Jonathon Pines

Type

Empty backbone

Tags

Venus

Expression

Mammalian

Available Since

Feb. 4, 2015

Availability

Academic Institutions and Nonprofits only

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IDG_CACNB1_OE_1

Plasmid

#161660

Purpose

Overexpresses CACNB1

Depositor

Mike McManus

Insert

CACNB1 (CACNB1 Human)

Tags

V5

Expression

Mammalian

Available Since

March 18, 2022

Availability

Academic Institutions and Nonprofits only

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pMGS7 (AAVS1sgRNA)

Plasmid

#126582

Purpose

A CRISPR/Cas9 construct to target the AAVS1 locus in humans

Depositor

Michael Guertin

Article

Sathyan et al Genes Dev. 2019 Oct 1;33(19-20):1441-145

Insert

AAVS1 sgRNA

Expression

Mammalian

Available Since

June 10, 2019

Availability

Academic Institutions and Nonprofits only

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ND4-DdCBE- left side TALE

Plasmid

#157844

Purpose

expresses left-side TALE–DddAtox half ND4-DdCBE in mammalian cells

Depositor

David Liu

Article

Mok et al Nature. 2020 Jul 8. pii: 10.1038/s41586-

Insert

SOD2 MTS–3x-HA–ND4 left tale–G1397 DddA-C–UGI–SOD2 3'UTR

Expression

Mammalian

Available Since

Aug. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pMSCV-PIG-miR-29b-1/miR-29a

Plasmid

#64231

Purpose

Expresses miR-29b-1 and miR-29a in mammalian cells

Depositor

Joshua Mendell

Article

Chang et al Nat Genet. 2008 Jan;40(1):43-50. Epub 20

Insert

miR-29b-1 and miR-29a (MIR29A, MIR29B1 Human)

Use

Retroviral

Expression

Mammalian

Available Since

June 2, 2015

Availability

Academic Institutions and Nonprofits only

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ND4-DdCBE-right side TALE

Plasmid

#157843

Purpose

expresses right-side TALE–DddAtox half ND4-DdCBE in mammalian cells

Depositor

David Liu

Article

Mok et al Nature. 2020 Jul 8. pii: 10.1038/s41586-

Insert

COX8A MTS–3xFLAG–ND4 right TALE–G1397 DddA-N–UGI–ATP5B 3'UTR

Expression

Mammalian

Available Since

Aug. 4, 2020

Availability

Academic Institutions and Nonprofits only

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EGFP-PIP5K1A

Plasmid

#202720

Purpose

expresses fluorescent protein-tagged enzyme

Depositor

Gerry Hammond

Article

Wills et al J Cell Sci. 2023 Aug 3:jcs.261494. doi:

Insert

EGFP:PIP5K1A (PIP5K1A Human)

Expression

Mammalian

Available Since

June 28, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-ERBB2-V5/HIS

Plasmid

#201103

Purpose

expression of human ERBB2 receptor tyrosine kinase in mammalian cells

Depositor

Pavel Krejčí

Article

Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

Insert

human ERBB2 receptor tyrosine kinase, full length, wildtype (ERBB2 Human)

Tags

V5/His

Expression

Mammalian

Promoter

CMV

Available Since

May 26, 2023

Availability

Academic Institutions and Nonprofits only

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pLV-U-TVA950

Plasmid

#115236

Purpose

Lentiviral transfer plasmid for expression of TVA950

Depositor

Ian Wickersham

Article

Jin et al Proc Natl Acad Sci U S A. 2023 Feb 14;12

Insert

TVA950

Expression

Mammalian

Available Since

April 21, 2021

Availability

Academic Institutions and Nonprofits only

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Halo-p58

Plasmid

#166896

Purpose

mammalian expression of p58 (ie ERGIC-53; LMAN1) tagged with HaloTag

Depositor

Jennifer Lippincott-Schwartz

Article

Weigel et al Cell. 2021 Apr 29;184(9):2412-2429.e16.

Insert

p58 (Lman1 Rat)

Tags

HaloTag

Expression

Mammalian

Mutation

L405Q, R419S- please see depositor comments

Promoter

CMV

Available Since

May 13, 2021

Availability

Academic Institutions and Nonprofits only

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DNA-PKcs N-terminal sgRNA

Plasmid

#207093

Purpose

pX330 based plasmid for expression of Cas9 and the AGCGGGACTCGGCGGCATGG sgRNA to target the DNA-PKcs locus.

Depositor

Jens Schmidt

Article

Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

Insert

AGCGGGACTCGGCGGCATGG

Expression

Mammalian

Promoter

CMV and U6

Available Since

April 30, 2025

Availability

Academic Institutions and Nonprofits only

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Sar1b-H79G-mOrange2

Plasmid

#166900

Purpose

mammalian expression of Sar1b-H79G tagged with mOrange2

Depositor

Jennifer Lippincott-Schwartz

Article

Weigel et al Cell. 2021 Apr 29;184(9):2412-2429.e16.

Insert

Sar1b

Tags

mOrange2

Expression

Mammalian

Mutation

H79G

Promoter

CMV

Available Since

April 14, 2021

Availability

Academic Institutions and Nonprofits only

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MMTV-cFOS-SV40

Plasmid

#19259

Depositor

Philip Leder

Insert

cFOS

Expression

Mammalian

Available Since

Sept. 15, 2008

Availability

Academic Institutions and Nonprofits only
pKeima-LiveDrop

Plasmid

#199695

Purpose

lipophagy reporter system based on the Keima-fluorophore

Depositor

Tobias Walther

Article

Chung et al Nat Cell Biol. 2023 Aug;25(8):1101-1110.

Insert

GPAT4 (Gpat4 Fly)

Tags

Keima

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 16, 2024

Availability

Academic Institutions and Nonprofits only

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DsRed-rab11 WT

Plasmid

#12679

Depositor

Richard Pagano

Article

Choudhury et al J Clin Invest. 2002 Jun . 109(12):1541-5

Insert

RAB11A (Homo sapiens) (RAB11A Human)

Tags

DsRed and HA

Expression

Mammalian

Available Since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only

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pCMV-AcGFP1-ORP9-PH

Plasmid

#214266

Purpose

Mammalian expression of green fluorescent PI4P reporter AcGFP1-ORP9-PH

Depositor

Shinya Tsukiji

Article

Ajiki et al RSC Chem Biol. 2024 Apr 24;5(6):544-555.

Insert

AcGFP1-ORP9(1-111) (Osbpl9 Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO_HaloTag7_LamB1

Plasmid

#175528

Purpose

Expression of HaloTag7 at the nuclear lamina of the mammalian cells

Depositor

Kai Johnsson

Article

Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

Insert

HaloTag7-LamB1

Expression

Mammalian

Available Since

Dec. 13, 2021

Availability

Academic Institutions and Nonprofits only

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SpCas9 TadDE

Plasmid

#193837

Purpose

Express TadDE (with SpCas9) in mammalian cells

Depositor

David Liu

Article

Neugebauer et al Nat Biotechnol. 2022 Nov 10. pii: 10.103

Insert

TadA-DualEditor-Cas9D10A-2xUGI

Expression

Mammalian

Available Since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pLVX-Tight-Puro_Nkx6.2

Plasmid

#64846

Purpose

A lentiviral vector for the expression of mouse Nkx6.2 under doxycyline control

Depositor

Paul Tesar

Article

Najm et al Nat Biotechnol. 2013 May;31(5):426-33. d

Insert

Nkx6.2 (Nkx6-2 Mouse)

Use

Lentiviral

Expression

Bacterial and Mammalian

Available Since

May 28, 2015

Availability

Academic Institutions and Nonprofits only

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pEMTB-mScarlet3-H (aka mYongHong)

Plasmid

#239407

Purpose

Expresses EMTB-mScarlet3-H in mammalian cells

Depositor

Zhifei Fu

Article

Xiong et al Nat Methods. 2025 Apr 17. doi: 10.1038/s

Insert

EMTB (MAP7 Human)

Tags

mScarlet3-H (aka mYongHong)

Expression

Mammalian

Mutation

M163H in mScarlet3

Promoter

CMV

Available Since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

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