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pcdna3.1 hygro mCherry

Plasmid

#128057

Purpose

Mammalian expression vector with N terminal mCherry

Depositor

Oskar Laur

Type

Empty backbone

Tags

mCherry

Expression

Mammalian

Available Since

Aug. 27, 2019

Availability

Academic Institutions and Nonprofits only

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pUB_smHA_KDM5B_MS2

Plasmid

#81085

Purpose

Translation reporter: Encodes KDM5B (1544 aa) fused to smHA (Spaghetti Monster HA: 10 copies of an epitope tag HA).

Depositor

Tim Stasevich

Article

Morisaki et al Science. 2016 Jun 17;352(6292):1425-9. d

Insert

smHA_KDM5B_24xMS2

Expression

Mammalian

Available Since

Sept. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1-FLAG-hSF3B1-K700E

Plasmid

#82577

Purpose

Expresses a codon-optimized ORF of human SF3B1 ( K700E mutant)

Depositor

Manoj Pillai

Article

Kesarwani et al Oncogene. 2016 Aug 15. doi: 10.1038/onc.

Insert

human SF3B1-K700E (SF3B1 Human)

Tags

FLAG peptide

Expression

Mammalian

Mutation

K700E

Promoter

CMV

Available Since

March 6, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3-sACE2(WT)-Fc(IgG1)

Plasmid

#145163

Purpose

Mammalian expression plasmid for soluble ACE2-Fc fusion (IgG1)

Depositor

Erik Procko

Article

Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

Insert

Angiotensin-converting enzyme 2 (ACE2 Human)

Tags

Fc region of human IgG1

Expression

Mammalian

Mutation

Extracellular, soluble protease domain (a.a. M1-D…

Promoter

CMV

Available Since

April 15, 2020

Availability

Academic Institutions and Nonprofits only

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THBS1-bio-His

Plasmid

#53417

Purpose

Expresses full-length Thrombospondin-1 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

Depositor

Gavin Wright

Article

Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

Insert

THBS1 (THBS1 Human)

Tags

His tag, enzymatic biotinylation sequence, and ra…

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 27, 2015

Availability

Academic Institutions and Nonprofits only

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pmScarlet3-LaminB_C1

Plasmid

#189776

Purpose

Construct for labelling nuclear envelopes in mammaiian cells using mScarlet3-LaminB fusion

Depositor

Dorus Gadella

Article

Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

Insert

mScarlet3-LaminB

Expression

Mammalian

Promoter

CMV

Available Since

March 21, 2023

Availability

Academic Institutions and Nonprofits only

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mAzurite-N1

Plasmid

#54617

Purpose

Localization: N1 Cloning Vector, Excitation: 384, Emission: 450

Depositor

Patrick Daugherty, Michael Davidson

Article

Mena et al Nat Biotechnol. 2006 Dec . 24(12):1569-7

Type

Empty backbone

Tags

mAzurite

Expression

Mammalian

Available Since

July 25, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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MSCV-CMV-DsRed-IRES-EGFP-DEST

Plasmid

#41941

Depositor

Stephen Elledge

Article

Yen et al Science. 2008 Nov 7;322(5903):918-23. do

Type

Empty backbone

Use

Retroviral

Expression

Mammalian

Available Since

Sept. 3, 2013

Availability

Academic Institutions and Nonprofits only

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pMD2.G-VSV-G-mut

Plasmid

#196508

Purpose

VSV-G mutant (K47Q, R354A)

Depositor

Howard Chang

Article

Yu et al Cell. 2022 Dec 22;185(26):4904-4920.e22.

Insert

VSV-G

Tags

NA

Expression

Mammalian

Mutation

K47Q, R354A

Promoter

CMV

Available Since

May 15, 2023

Availability

Academic Institutions and Nonprofits only

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pMIIS948-CXCR4

Plasmid

#238213

Purpose

TasR tigRNA expression to target CXCR4 under U6 promoter in human cells.

Depositor

Feng Zhang

Article

Faure et al Science. 2025 Feb 27:eadv9789. doi: 10.1

Insert

tigRNA

Expression

Mammalian

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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pU6-(BbsI)_CBh-Cas9-T2A-BFP

Plasmid

#64323

Purpose

Expression vector for sgRNAs cloned into the BbsI sites and for expression of Cas9 linked to BFP via a T2A peptide

Depositor

Ralf Kuehn

Article

Chu et al Nat Biotechnol. 2015 Mar 24. doi: 10.103

Inserts

Cas9
sgRNA cassette

Use

CRISPR

Tags

3xFLAG, NLS, and T2A-EBFP2

Expression

Mammalian

Promoter

CBh and U6

Available Since

May 29, 2015

Availability

Academic Institutions and Nonprofits only

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pEGFP‐RNASEH1

Plasmid

#108699

Purpose

For expression of C-terminally eGFP-tagged human RNASEH1 (nuclear isoform) in mammalian cells

Depositor

Andrew Jackson, Martin Reijns

Article

Bubeck et al Nucleic Acids Res. 2011 May;39(9):3652-6

Insert

RNASEH1 (nuclear isoform) (RNASEH1 Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

April 24, 2018

Availability

Academic Institutions and Nonprofits only

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pDONR221-SLC45A4_STOP

Plasmid

#161345

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC45A4 (SLC45A4 Human)

Expression

Mammalian

Available Since

Sept. 15, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR221-SLC25A45_STOP

Plasmid

#161220

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC25A45 (SLC25A45 Human)

Expression

Mammalian

Available Since

June 21, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR221-SLC18B1_STOP

Plasmid

#161187

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC18B1 (C6orf192 Human)

Expression

Mammalian

Available Since

June 21, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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GATA3-pCDNA3.1

Plasmid

#115271

Purpose

Expresses human GATA3 in mammalian cells

Depositor

Arne S. Schaefer

Insert

GATA3 (GATA3 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 10, 2018

Availability

Academic Institutions and Nonprofits only

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PGK plasmid

Plasmid

#169744

Purpose

Unregulated "open-loop" PGK promoter plasmid. This plasmid encodes eGFP to report circuit output levels.

Depositor

Francois St-Pierre

Article

Yang et al Nat Commun. 2021 Jul 5;12(1):4132. doi:

Insert

eGFP

Use

Synthetic Biology

Expression

Mammalian

Promoter

PGK promoter

Available Since

June 9, 2021

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1-Flag-humanRBM47

Plasmid

#134607

Purpose

Plasmid bearing RBM47 coding sequence. Flag tagged is useful for IP and WB assays.

Depositor

Silvestro Conticello

Article

Chieca et al bioRxiv 2021

Insert

Flag-humanRBM47 (RBM47 Human)

Tags

flag

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 16, 2019

Availability

Academic Institutions and Nonprofits only

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secretedBirA-8his

Plasmid

#32408

Depositor

Gavin Wright

Article

Bushell et al Genome Res. 2008 Apr;18(4):622-30. Epub

Insert

BirA

Tags

8 His tag

Expression

Mammalian

Available Since

Jan. 17, 2012

Availability

Academic Institutions and Nonprofits only

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pEGFP-NR1a

Plasmid

#17926

Depositor

Stefano Vicini

Article

Luo et al Neuropharmacology. 2002 Mar . 42(3):306-

Insert

glutamate receptor, ionotropic, N-methyl D-aspartate-like 1A (Grinl1a Rat)

Tags

eGFP

Expression

Mammalian

Available Since

June 10, 2013

Availability

Academic Institutions and Nonprofits only

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