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pCDNA5 TEX264wt-cGFP

Plasmid

#220211

Purpose

Mammalian expression of wild type TEX264 fused at C-terminus to GFP

Depositor

Kristijan Ramadan

Article

Fielden et al Nat Commun. 2020 Mar 9;11(1):1274. doi:

Insert

TEX264 (TEX264 Human)

Tags

Strep-strep-HA

Expression

Mammalian

Promoter

CMV/TetO

Available Since

June 12, 2024

Availability

Academic Institutions and Nonprofits only

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pHaloTag-EGFP

Plasmid

#86629

Purpose

Expresses HaloTag-eGFP tandem (cytosolic)

Depositor

Thomas Leonard, Ivan Yudushkin

Article

Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

Insert

HaloTag

Expression

Mammalian

Available Since

March 7, 2017

Availability

Academic Institutions and Nonprofits only

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pIRE4-Azi

Plasmid

#105829

Purpose

Plasmid encoding for the translational pair that enables efficent incoporation of p-Azido-phenylalanine in response to the amber codon into proteins in mammmalian cells

Depositor

Irene Coin

Article

Seidel et al Elife. 2017 Aug 3;6. doi: 10.7554/eLife.

Inserts

Humanized EAziRS
4 copies of the U6-BstYam tRNA cassette

Expression

Mammalian

Mutation

(Y37L, D182S, F183M, L186A, D265R)TyrRS

Promoter

PGK and U6

Available Since

March 15, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV-mCherry-MHC-IIA

Plasmid

#35687

Depositor

Venkaiah Betapudi

Article

Dulyaninova et al Mol Biol Cell. 2007 Aug;18(8):3144-55. E

Insert

MHC IIA (MYH9 Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

April 24, 2012

Availability

Academic Institutions and Nonprofits only

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pDEST-CMV-N-mCherry

Plasmid

#123215

Purpose

N-terminal mCherry tag. Gateway destination vector for mammalian expression.

Depositor

Robin Ketteler

Article

Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

Type

Empty backbone

Use

Gateway Cloning

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 14, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.3-NL63-D14

Plasmid

#172666

Purpose

encodes NL63 spike protein, for pseudovirus production

Depositor

David Nemazee

Article

Dacon et al Science. 2022 Jul 12:eabq3773. doi: 10.1

Insert

HCNV63gp2 (HCNV63gp2 human coronavirus NL63)

Use

Lentiviral and Retroviral

Tags

NA

Expression

Mammalian

Mutation

c-terminal deletion of 14aa

Promoter

CMV

Available Since

Jan. 28, 2022

Availability

Academic Institutions and Nonprofits only

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mCherry-Alpha-Actinin-19

Plasmid

#54975

Purpose

Localization: Alpha Actinin, Excitation: 587, Emission: 610

Depositor

Michael Davidson

Insert

actinin alpha 1 (ACTN1 Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 17, 2014

Availability

Academic Institutions and Nonprofits only

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TagRFP-T-EEA1

Plasmid

#42635

Depositor

Silvia Corvera

Article

Navaroli et al Proc Natl Acad Sci U S A. 2012 Feb 21;10

Insert

EEA1 (EEA1 Human)

Tags

TagRFP-T

Expression

Mammalian

Mutation

none

Promoter

CMV

Available Since

March 1, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA3.4_7C11-1 light chain

Plasmid

#198252

Purpose

recombinant expression of light chain of monoclonal anti-AMP antibody 7C11-1

Depositor

Aymelt Itzen

Article

Hopfner et al iScience. 2020 Nov 13;23(12):101800. doi

Insert

light chain of monoclonal anti-AMP antibody 7C11-1 (mouse kappa)

Tags

IL10 signal peptide

Expression

Mammalian

Promoter

CMV

Available Since

May 5, 2023

Availability

Academic Institutions and Nonprofits only

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pHCMV_WNV_PrME

Plasmid

#207313

Purpose

Mammalian expression of West Nile Virus PrME protein

Depositor

Feng Zhang

Article

Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

Insert

WNV_PrME (POLY West Nile Virus)

Expression

Mammalian

Mutation

mammalian codon optimization

Promoter

CMV promoter

Available Since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV-mGold2s-Tubulin-C-18

Plasmid

#231771

Purpose

Visualization of tubulin in mammalian cells using mGold2s

Depositor

Francois St-Pierre

Article

Lee et al Nat Commun. 2025 Apr 4;16(1):3241. doi:

Insert

mGold2s-Tubulin-C-18 (TUBA1B Human)

Tags

mGold2s

Expression

Mammalian

Promoter

CMV

Available Since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA-NES-PhoCl-mCherry

Plasmid

#87690

Purpose

Express NES-PhoCl-mCherry in mammalian cells

Depositor

Robert Campbell

Article

Zhang et al Nat Methods. 2017 Mar 13. doi: 10.1038/n

Insert

NES-PhoCl-mCherry

Tags

nuclear export signal

Expression

Mammalian

Promoter

CMV

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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dCas9-hHDAC3

Plasmid

#98591

Purpose

3rd generation lenti vector encoding dCas9-hHDAC3 (EF1a-NLS-dCas9(N863)-hHDAC3). Expresses dCas9 fused to human HDAC3.

Depositor

Zhaolan Zhou

Article

Kwon et al Nat Commun. 2017 May 12;8:15315. doi: 10

Insert

dCAS9-HDAC3 (HDAC3 S. pyogenes, Human, Synthetic)

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

D10A and N863A in Cas9

Promoter

EF1A

Available Since

Oct. 3, 2017

Availability

Academic Institutions and Nonprofits only

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pLL7.0: tgRFPt-SSPB WT

Plasmid

#60415

Purpose

Mammalian Expression of tgRFPt-Nano

Depositor

Brian Kuhlman

Article

Guntas et al Proc Natl Acad Sci U S A. 2015 Jan 6;112

Insert

tgRFPt-SSPB

Use

Cre/Lox and Lentiviral

Tags

TagRFPt

Expression

Mammalian

Mutation

Y11K and A15E

Promoter

CMV

Available Since

Feb. 27, 2015

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1-hAIM2

Plasmid

#51542

Purpose

Express GFP-tagged hAIM2 in mammalian cells

Depositor

Emad Alnemri

Article

Fernandes-Alnemri et al Nature. 2009 Mar 26;458(7237):509-13. do

Insert

AIM2 (AIM2 Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

March 19, 2014

Availability

Academic Institutions and Nonprofits only

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pCMV-PE7-P2A-BSD

Plasmid

#224122

Purpose

Mammalian expression of SpCas9 PE7 prime editor with P2A-BSD marker

Depositor

Brittany Adamson

Article

Yan et al Nature. 2024 Apr 3. doi: 10.1038/s41586-

Insert

PE7-P2A-BSD

Tags

P2A-BSD, SV40 bpNLS, and c-Myc NLS

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 19, 2024

Availability

Academic Institutions and Nonprofits only

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SPLICS Po-Mt Long P2A

Plasmid

#164115

Purpose

Detect the long Peroxisomes-mitochondria contact

Depositor

Marisa Brini, Tito Calì

Article

Vallese et al Nat Commun. 2020 Nov 27;11(1):6069. doi:

Insert

Split GFP Po-Mt Long P2A

Expression

Mammalian

Available Since

Feb. 2, 2021

Availability

Academic Institutions and Nonprofits only

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EGFP

Plasmid

#176015

Purpose

Mammalian expression of EGFP. Parton lab clone DFS

Depositor

Rob Parton

Article

Lo et al J Cell Biol. 2021 Dec 6;220(12). pii: 21

Insert

EGFP

Expression

Mammalian

Available Since

Nov. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV_hSyn1_HyPer7_mito

Plasmid

#238968

Purpose

Mammalian neurons HyPer7 expression targeted to the mitochondrial matrix

Depositor

Dmitry Bilan

Article

Kelmanson et al Redox Biol. 2021 Nov 3;48:102178. doi: 1

Insert

HyPer7

Use

AAV

Tags

3x Cytochrome C Oxidase Subunit VIII presequense

Expression

Mammalian

Promoter

hSyn1

Available Since

Aug. 15, 2025

Availability

Academic Institutions and Nonprofits only

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Flag-HA-GFP

Plasmid

#22612

Depositor

Wade Harper

Article

Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

Insert

GFP

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

None

Available Since

Dec. 4, 2009

Availability

Academic Institutions and Nonprofits only

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