Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

79,276 results

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pAAV-CAG-DIO-TurboID

Plasmid

#237885

Purpose

AAV vector for Cre-dependent expression of TurboID

Depositor

Rui Lin

Article

Zhong et al Neuron. 2025 Sep 18:S0896-6273(25)00657-

Insert

TurboID

Use

AAV and Cre/Lox

Tags

FLAG tag

Expression

Mammalian

Promoter

CAG

Available Since

Aug. 25, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV sfGFP-CHMP2B-3x-55-96

Plasmid

#232015

Purpose

Expression of 3 tandem copies of CHMP2B helix 2, connected with gly-ser linkers, attached to sfGFP.

Depositor

Franz-Ulrich Hartl

Article

Sitron et al Mol Cell. 2025 Sep 18;85(18):3505-3523.e

Insert

CHMP2B (CHMP2B Human)

Expression

Mammalian

Available Since

Aug. 22, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV sfGFP-CHMP2B-201-213

Plasmid

#232016

Purpose

Expression of the CHMP2B VPS4-binding region attached to sfGFP.

Depositor

Franz-Ulrich Hartl

Article

Sitron et al Mol Cell. 2025 Sep 18;85(18):3505-3523.e

Insert

CHMP2B (CHMP2B Human)

Tags

HA

Expression

Mammalian

Available Since

Aug. 22, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV CHMP2B-L4D F5D-3XHA

Plasmid

#232003

Purpose

Expression of a CHMP2B membrane binding mutant (L4D F5D) with a 3xHA tag.

Depositor

Franz-Ulrich Hartl

Article

Sitron et al Mol Cell. 2025 Sep 18;85(18):3505-3523.e

Insert

CHMP2B (CHMP2B Human)

Tags

3xHA

Expression

Mammalian

Mutation

L4D F5D

Available Since

Aug. 22, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV CHMP2B-L4D F5D-d10-52-3xHA (Δα1)

Plasmid

#232004

Purpose

Expression of a CHMP2B membrane binding mutant (L4D F5D) with the first alpha helix deleted with a 3xHA tag.

Depositor

Franz-Ulrich Hartl

Article

Sitron et al Mol Cell. 2025 Sep 18;85(18):3505-3523.e

Insert

CHMP2B (CHMP2B Human)

Tags

3xHA

Expression

Mammalian

Mutation

L4D F5D Δ10-52

Available Since

Aug. 22, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV CHMP2B-L4D F5D-d55-96-3xHA (Δα2)

Plasmid

#232005

Purpose

Expression of a CHMP2B membrane binding mutant (L4D F5D) with the second alpha helix deleted with a 3xHA tag.

Depositor

Franz-Ulrich Hartl

Article

Sitron et al Mol Cell. 2025 Sep 18;85(18):3505-3523.e

Insert

CHMP2B (CHMP2B Human)

Tags

3xHA

Expression

Mammalian

Mutation

L4D F5D Δ55-96

Available Since

Aug. 22, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV CHMP2B-L4D F5D-d97-106-3xHA (Δα2/3 loop)

Plasmid

#232006

Purpose

Expression of a CHMP2B membrane binding mutant (L4D F5D) with the loop connecting the second and third alpha helices deleted with a 3xHA tag.

Depositor

Franz-Ulrich Hartl

Article

Sitron et al Mol Cell. 2025 Sep 18;85(18):3505-3523.e

Insert

CHMP2B (CHMP2B Human)

Tags

3xHA

Expression

Mammalian

Mutation

L4D F5D Δ97-106

Available Since

Aug. 22, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV CHMP2B-L4D F5D-d106-113-3xHA (Δα3)

Plasmid

#232007

Purpose

Expression of a CHMP2B membrane binding mutant (L4D F5D) with the third alpha helix deleted with a 3xHA tag.

Depositor

Franz-Ulrich Hartl

Article

Sitron et al Mol Cell. 2025 Sep 18;85(18):3505-3523.e

Insert

CHMP2B (CHMP2B Human)

Tags

3xHA

Expression

Mammalian

Mutation

L4D F5D Δ106-113

Available Since

Aug. 22, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV CHMP2B-L4D F5D-d118-138-3xHA (Δα4)

Plasmid

#232008

Purpose

Expression of a CHMP2B membrane binding mutant (L4D F5D) with the fourth alpha helix deleted with a 3xHA tag.

Depositor

Franz-Ulrich Hartl

Article

Sitron et al Mol Cell. 2025 Sep 18;85(18):3505-3523.e

Insert

CHMP2B (CHMP2B Human)

Tags

3xHA

Expression

Mammalian

Mutation

L4D F5D Δ118-138

Available Since

Aug. 22, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV CHMP2B-L4D F5D-d55-138-3xHA (Δα2-4)

Plasmid

#232009

Purpose

Expression of a CHMP2B membrane binding mutant (L4D F5D) with the second through fourth helices deleted with a 3xHA tag.

Depositor

Franz-Ulrich Hartl

Article

Sitron et al Mol Cell. 2025 Sep 18;85(18):3505-3523.e

Insert

CHMP2B (CHMP2B Human)

Tags

3xHA

Expression

Mammalian

Mutation

L4D F5D Δ55-138

Available Since

Aug. 22, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV CHMP2B-L4D F5D-d159-174-3xHA (Δα5)

Plasmid

#232010

Purpose

Expression of a CHMP2B membrane binding mutant (L4D F5D) with the fifth alpha helix deleted with a 3xHA tag.

Depositor

Franz-Ulrich Hartl

Article

Sitron et al Mol Cell. 2025 Sep 18;85(18):3505-3523.e

Insert

CHMP2B (CHMP2B Human)

Tags

3xHA

Expression

Mammalian

Mutation

L4D F5D Δ159-174

Available Since

Aug. 22, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV CHMP2B-L4D F5D-d201-213-HA (ΔVPS4 binding)

Plasmid

#232011

Purpose

Expression of a CHMP2B membrane binding mutant (L4D F5D) with the VPS4-binding region deleted with a 3xHA tag.

Depositor

Franz-Ulrich Hartl

Article

Sitron et al Mol Cell. 2025 Sep 18;85(18):3505-3523.e

Insert

CHMP2B (CHMP2B Human)

Tags

HA

Expression

Mammalian

Mutation

L4D F5D Δ201-213

Available Since

Aug. 22, 2025

Availability

Academic Institutions and Nonprofits only

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pEF1a FLAG-CHMP2B

Plasmid

#232012

Purpose

Expression of FLAG-tagged CHMP2B

Depositor

Franz-Ulrich Hartl

Article

Sitron et al Mol Cell. 2025 Sep 18;85(18):3505-3523.e

Insert

CHMP2B (CHMP2B Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Available Since

Aug. 22, 2025

Availability

Academic Institutions and Nonprofits only

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pEF1a FLAG-CHMP2B-d55-96

Plasmid

#232013

Purpose

Expression of FLAG-tagged CHMP2B with the second alpha helix deleted.

Depositor

Franz-Ulrich Hartl

Article

Sitron et al Mol Cell. 2025 Sep 18;85(18):3505-3523.e

Insert

CHMP2B (CHMP2B Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

Δ55-96

Available Since

Aug. 22, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV sfGFP-CHMP2B-55-96

Plasmid

#232014

Purpose

Expression of CHMP2B helix 2 attached to sfGFP.

Depositor

Franz-Ulrich Hartl

Article

Sitron et al Mol Cell. 2025 Sep 18;85(18):3505-3523.e

Insert

CHMP2B (CHMP2B Human)

Expression

Mammalian

Available Since

Aug. 22, 2025

Availability

Academic Institutions and Nonprofits only

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NanoLuc-ACT1 Fusion Vector

Plasmid

#237018

Purpose

Express NanoLuc(R)-ACT1 Fusion Protein in Mammalian Cells under a CMV promoter

Depositor

Promega Corporation

Has Service

DNA

Insert

ACT1 (TRAF3IP2 Human)

Tags

NanoLuc (R)

Expression

Mammalian

Mutation

None

Promoter

CMV

Available Since

July 14, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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HA-MAP4K1

Plasmid

#229396

Purpose

Lentiviral or overpexression of cDNA

Depositor

Christopher Vakoc

Article

Klingbeil et al Cancer Discov. 2024 Jul 26. doi: 10.1158

Insert

MAP4K1 (MAP4K1 Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Promoter

EFS

Available Since

Dec. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA-DDX53-FLAG

Plasmid

#222241

Purpose

Express FLAG tagged DDX53 in mammalian cells

Depositor

Yuliang Wu

Insert

DDX53 (DDX53 Human)

Tags

3xFLAG

Expression

Mammalian

Promoter

T7

Available Since

Aug. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA-DDX53-GFP

Plasmid

#222239

Purpose

Express GFP tagged DDX53 in mammalian cells

Depositor

Yuliang Wu

Insert

DDX53 (DDX53 Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV and T7

Available Since

Aug. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pPYRMandi-ABI

Plasmid

#233649

Purpose

Cellular colocalization assay (PYRMandi/ABI) for the CIP Mandi (mandipropamid)

Depositor

Richard Wombacher

Article

Poschko et al ACS Chem Biol. 2025 Jan 27. doi: 10.1021

Insert

TOMM20-mCherry-PYRMandi-P2A-eGFP-ABI

Expression

Mammalian

Promoter

CMV

Available Since

April 9, 2025

Availability

Academic Institutions and Nonprofits only

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