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80,773 results

Showing: 7701 - 7720 of 80773 results

  1. IABE

    Plasmid
    #247332
    Purpose
    Vector encoding human codon-optimized engineered DelIscB nickase fusion with TadA8e driven by CAG promoter, optimized sgRNA (sgRNA-V5) driven by hU6, and mCherry driven by CMV promoter
    Depositor
    Yong Tian
    Insert
    pCAG_enDelIscB(D60A)-2×TadA8e(V106W)_npNLS_polyA_pU6_sgRNA_V5-BsaI_pCMV_mCherry
    Use
    CRISPR
    Expression
    Mammalian
    Available Since
    Jan. 7, 2026
    Availability
    Academic Institutions and Nonprofits only
    247332-plasmid-map-sequence-id-496501

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  2. ICBE

    Plasmid
    #247331
    Purpose
    Vector encoding human codon-optimized engineered DelIscB nickase fusion with APOBEC3A and UGI driven by CAG promoter, optimized sgRNA (sgRNA-V5) driven by hU6, and mCherry driven by CMV promoter
    Depositor
    Yong Tian
    Insert
    pCAG_enDelIscB(D60A)-hAPOBEC3A(W98Y/W104A/Y130F)-2×UGI_npNLS_polyA_pU6_sgRNA_V5-BsaI_pCMV_mCherry
    Expression
    Mammalian
    Available Since
    Jan. 7, 2026
    Availability
    Academic Institutions and Nonprofits only
    247331-plasmid-map-sequence-id-496500

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  3. pCAG-denAsCas12a(E174R/S542R/K548R/D908A)-NLS(nuc)-3xHA-VPR (RTW776)

    Plasmid
    #107943
    Purpose
    Mammalian expression plasmid for human codon optimized DNase-inactive enAsCas12a (enhanced AsCas12a) fused to the VPR activation domain, encoding E174R/S542R/K548R/D908A substitutions
    Depositor
    Keith Joung, Benjamin Kleinstiver
    Article
    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103
    Insert
    human codon optimized DNase-inactive (D908A) enAsCas12a (E174R/S542R/K548R) fused to VPR activation domain
    Tags
    3x HA, NLS (nucleoplasmin), and VPR (VP64-p65-Rta…
    Expression
    Mammalian
    Mutation
    E174R, S542R, K548R and D908A
    Promoter
    CAG
    Available Since
    Feb. 11, 2019
    Availability
    Academic Institutions and Nonprofits only
    107943-plasmid-map-sequence-id-207614

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  5. pDAC439

    Plasmid
    #195237
    Purpose
    Mammalian expression of Csm complex from single promoter; RFP backbone (Used for RNA knockdown)
    Depositor
    Jennifer Doudna
    Article
    Colognori et al Nat Biotechnol. 2023 Jan 23. doi: 10.103
    Insert
    FLAG-NLS-Csm5-2A-FLAG-NLS-Csm4-2A-FLAG-NLS-Csm3-2A-FLAG-NLS-Csm2; RFP-2A-FLAG-NLS-Cas6-2A-FLAG-NLS-Csm1; crRNA
    Expression
    Mammalian
    Available Since
    Jan. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    195237-plasmid-map-sequence-id-382945

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  7. pAG CMV Empty Puro

    Plasmid
    #237881
    Purpose
    Empty mammalian expression plasmid for cloning untagged proteins
    Depositor
    Meghan Rego, Addgene Research Program
    Type
    Empty backbone
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    June 12, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    237881-plasmid-map-sequence-id-469912

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  9. HiBiT-TP53 Fusion Vector

    Plasmid
    #236885
    Purpose
    Express HiBiT-TP53 in Mammalian Cells under a CMV promoter
    Depositor
    Promega Corporation
    Has Service
    DNA
    Insert
    TP53 (TP53 Human)
    Tags
    HiBiT
    Expression
    Mammalian
    Mutation
    None
    Promoter
    CMV
    Available Since
    July 14, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    236885-plasmid-map-sequence-id-473395

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  19. PB-TAC-ERP2

    Plasmid
    #80478
    Purpose
    All-in-One piggyBac transposon Destination vector for dox-inducible expression of Gateway cloned elements (inducible mCherry and constitutive rtTA and puromycin resistance)
    Depositor
    Knut Woltjen
    Article
    Kim et al Methods Mol Biol. 2016;1357:111-31. doi:
    Type
    Empty backbone
    Use
    Piggybac transposon
    Expression
    Mammalian
    Available Since
    Nov. 28, 2016
    Availability
    Academic Institutions and Nonprofits only
    80478-plasmid-map-sequence-id-404034

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  20. pcDNA3.1-Mfn1

    Plasmid
    #191955
    Purpose
    Expression of Mfn1, a mediator of mitochondrial fusion.
    Depositor
    Luca Scorrano
    Insert
    Mfn1
    Expression
    Mammalian
    Available Since
    Dec. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    191955-plasmid-map-sequence-id-379811

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Showing: 7701 - 7720 of 80773 results