75,771 results

1. pAAV.CAG.Flex.GCaMP6s.WPRE.SV40

   Plasmid

   #100842

   Purpose

   AAV expression of Cre recombinase-activated ultrasensitive protein calcium sensor from CAG promoter

   Depositor

   GENIE Project, Douglas Kim

   Article

   Chen et al Nature. 2013 Jul 18;499(7458):295-300. d

   Has Service

   AAV1, AAV5, and AAV9

   Insert

   GCaMP6s
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   GCaMP3-K78H T302L R303P D380Y T381R S383T R392G

   Promoter

   CAG

   Available since

   Feb. 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pZac2.1-GfaABC1D-lck-jGCaMP8s

   Plasmid

   #176761

   Purpose

   AAV-mediated expression of membrane bound GCaMP8s under the control of GfaABC1D promoter

   Depositor

   Loren Looger

   Has Service

   AAV1 and AAV9

   Insert

   lck-jGCaMP8s
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   GfaABC1D

   Available since

   Nov. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. EGFP-Rab13

   Plasmid

   #110500

   Purpose

   Fluorescent Rab13

   Depositor

   Bernardo Mainou

   Article

   Mainou et al J Virol. 2012 Aug;86(16):8346-58. doi: 1

   Insert

   Rab13 (RAB13 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pAAV.RMacL3-01.EGFP.BCA5.WPRE

   Plasmid

   #225685

   Purpose

   L3 pyramidal neuron Enhancer to drive transgene expression

   Depositor

   William Stauffer

   Article

   He et al Neuron. 2025 May 21;113(10):1548-1561.e8

   Insert

   RMacL3-01.EGFP.BCA5
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 30, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. dCas9-10xSunTag-P2A-tagBFP

   Plasmid

   #174141

   Purpose

   Expresses the dCas9-10xSunTag-tagBFP fusion protein for the recruitment of scFv(GCN4)-fused effector domains. A shorter version of the Addgene 60903 plasmid.

   Depositor

   Albert Jeltsch

   Article

   Hofacker et al Int J Mol Sci. 2020 Jan 13;21(2). pii: i

   Insert

   dCas9, 10xSunTag, tagBFP
   

   Use

   Tags

   HA and tagBFP

   Expression

   Mammalian

   Mutation

   Promoter

   SV40

   Available since

   Oct. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCCLc-MNDU3-Luciferase-PGK-EGFP-WPRE

   Plasmid

   #89608

   Purpose

   Expression of Luciferase in human cells. 3rd generation lentiviral vector

   Depositor

   Fernando Fierro

   Article

   Beegle et al Stem Cells. 2015 Jun;33(6):1818-28. doi:

   Insert

   Luciferase
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   MNDU3

   Available since

   July 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pU6-cj-E sgRNA

   Plasmid

   #169915

   Purpose

   Expression of sgRNA on an optimized gRNA scaffold contains A-U pair flip stabilize the CjCas9/sgRNA complex.

   Depositor

   Zhili Rong

   Article

   Zhang et al Nucleic Acids Res. 2021 Mar 22. pii: 617

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pEGFR-PATagRFP

   Plasmid

   #31950

   Depositor

   Vladislav Verkhusha

   Article

   Subach et al J Am Chem Soc. 2010 May 12;132(18):6481-

   Insert

   PATagRFP
   

   Use

   Tags

   EGF receptor (EGFR)

   Expression

   Mammalian

   Mutation

   R69S/F84W/Q139K/A147P/N148S/M151K/Y153K/S165V/H20…

   Promoter

   CMV

   Available since

   Oct. 28, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. Lamp1-YFP

   Plasmid

   #1816

   Purpose

   Mammalian expression of Lamp1 fused to YFP. Used for localization to the lysosome.

   Depositor

   Walther Mothes

   Article

   Sherer et al Traffic 2003 Nov;4(11):785-801.

   Insert

   lysosome associated membrane protein 1 (Lamp1 Rat)
   

   Use

   Tags

   YFP

   Expression

   Mammalian

   Mutation

   D50E (not important for function of plasmid)

   Promoter

   Available since

   Feb. 16, 2006

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. NES-mCherry-cPHx3

    Plasmid

    #116862

    Purpose

    PI(3,4)P2 biosensor

    Depositor

    Gerry Hammond

    Article

    Goulden et al J Cell Biol. 2019 Mar 4;218(3):1066-1079

    Insert

    PLEKHA1 (PLEKHA1 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    amino acids 169-329 (tandem trimer)

    Promoter

    Available since

    Oct. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pPGKPhiC31obpA

    Plasmid

    #13795

    Purpose

    Expression of mouse codon-optimized PhiC31 recombinase with bovine growth hormone polyadenylation (bpA) sequence.

    Depositor

    Philippe Soriano

    Article

    Raymond et al PLoS ONE. 2007 . 2():e162.

    Insert

    PhiC31o
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PGK

    Available since

    Jan. 16, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pX459V2.0-eSpCas9(1.1)

    Plasmid

    #108292

    Purpose

    pX459 V2.0 (Plasmid #62988) with the K848A, K1003A, and R1060A mutations

    Depositor

    Yuichiro Miyaoka

    Article

    Kato-Inui et al Nucleic Acids Res. 2018 May 18;46(9):467

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCXLE-SOX2-p2a-KLF4

    Plasmid

    #193292

    Purpose

    episomal hSK (expressing human SOX2 + KLF4)

    Depositor

    Hans Schöler

    Article

    MacCarthy et al Cell Stem Cell. 2024 Jan 4;31(1):127-147

    Insert

    SOX2-p2a-KLF4 (SOX2, KLF4 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    F2A of original pCXLE-SK vector was replaced with…

    Promoter

    CAG

    Available since

    Dec. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pAAV.hSyn.FLEX.iGluSnFR3.v857.GPI.codonopt

    Plasmid

    #175181

    Purpose

    Fluorescent reporter for glutamate, third generation, variant 857 in GPI backbone. iGluSnFR3.v857.GPI

    Depositor

    Kaspar Podgorski

    Article

    Aggarwal et al Nat Methods. 2023 May 4. doi: 10.1038/s4

    Has Service

    AAV1

    Insert

    pAAV hSyn FLEX iGluSnFR3 v857.GPI
    

    Use

    AAV, Cre/Lox, Mouse Targeting, and Synthetic Biol…

    Tags

    GPI Anchor, IgK-chain, and Myc epi tag

    Expression

    Mammalian

    Mutation

    Promoter

    human Synapsin

    Available since

    Oct. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. NES-Grx1-roGFP2

    Plasmid

    #236551

    Purpose

    Expressing fluorescent Grx1-roGFP2 fusion protein in mammalian cells with localization in the cytoplasm.

    Depositor

    Pablo Rivera-Fuentes

    Insert

    NES-Grx1-roGFP2 (NES Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pPBT/EFS-Cas9-PuroTk

    Plasmid

    #170542

    Purpose

    Expresses spCas9 with a P2A junction to the puromycin resistance gene directly fused to the thymidine kinase

    Depositor

    Jacob Giehm Mikkelsen

    Article

    Thomsen et al Mol Ther Nucleic Acids. 2022 Aug 4;29:56

    Insert

    PuroTK
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. PCDNA3-GFP1-9 T2A mCherry

    Plasmid

    #124430

    Purpose

    Expresses GFP1-9 and T2A mCherry in mammalian cells

    Depositor

    Xiaokun Shu

    Article

    Zhang et al J Am Chem Soc. 2019 Mar 1. doi: 10.1021/

    Insert

    GFP1-9 T2A mCherry
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pDEST26-C-FLAG

    Plasmid

    #79275

    Purpose

    Destination vector containing a C-terminal FLAG tag

    Depositor

    Rita Shiang

    Article

    Amr et al Am J Hum Genet. 2007 Oct;81(4):673-83. E

    Insert

    FLAG
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 13, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

19. RelB cFlag pcDNA3

    Plasmid

    #20017

    Depositor

    Stephen Smale

    Insert

    RelB (Relb Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 28, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. L444P GBA pcDNA3.1

    Plasmid

    #188582

    Purpose

    Ectopic expression L444P GBA

    Depositor

    Anthony Schapira

    Article

    Smith et al Hum Mol Genet. 2022 Sep 20:ddac233. doi:

    Insert

    GBA (GBA1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    L444P

    Promoter

    CMV

    Available since

    Dec. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge