78,128 results

1. pDONR221_SLC25A31

   Plasmid

   #131969

   Purpose

   Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

   Depositor

   RESOLUTE Consortium, Giulio Superti-Furga

   Article

   Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

   Insert

   SLC25A31 (SLC25A31 Human)
   

   Expression

   Mammalian

   Available Since

   Oct. 21, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. pNLS-iRFP713

   Plasmid

   #45468

   Depositor

   Vladislav Verkhusha

   Article

   Shcherbakova et al Nat Methods. 2013 Jun 16. doi: 10.1038/n

   Insert

   iRFP713
   

   Tags

   nuclear localization signal

   Expression

   Mammalian

   Mutation

   T2A, S13L, A92T, V104T, V114I, E161K, E193K, F198…

   Promoter

   CMV

   Available Since

   June 17, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. CCR4-Tango

   Plasmid

   #66241

   Purpose

   Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

   Depositor

   Bryan Roth

   Article

   Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

   Insert

   CCR4 (CCR4 Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 15, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAmCyan1-C1-ADCY6

   Plasmid

   #113905

   Purpose

   N-terminal Cyan Fluorescent tag in Adenylate Cyclase 6

   Depositor

   Anjaparavanda Naren

   Article

   Thomas et al J Biol Chem. 2018 Jun 14. pii: RA118.003

   Insert

   Adenylate Cyclase 6 (ADCY6 Human)
   

   Tags

   Cyan

   Expression

   Bacterial and Mammalian

   Promoter

   pCMV

   Available Since

   Oct. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLY005SB_pAAV-U6sg(BbsI)-EFS-SB100X

   Plasmid

   #192148

   Purpose

   T cell CRISPR AAV vector

   Depositor

   Sidi Chen

   Article

   Dong et al Cell. 2019 Aug 22;178(5):1189-1204.e23.

   Type

   Empty backbone

   Use

   AAV

   Expression

   Mammalian

   Mutation

   NA

   Available Since

   Nov. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. TFORF1027

   Plasmid

   #141784

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   RARA (RARA Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Jan. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. p4489 Flag-betaTrCP

   Plasmid

   #10865

   Depositor

   Peter Howley

   Article

   Zhou et al Mol Cell. 2000 Sep . 6(3):751-6.

   Insert

   betaTrCP (BTRC Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Available Since

   Aug. 3, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMV-myc-Atg7

   Plasmid

   #24921

   Depositor

   Toren Finkel

   Article

   Lee et al Proc Natl Acad Sci U S A. 2008 Mar 4. 10

   Insert

   Atg7 (ATG7 Human)
   

   Tags

   Myc

   Expression

   Mammalian

   Available Since

   June 10, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEGFP-N1 human cofilin WT

   Plasmid

   #50859

   Purpose

   expresses human cofilin fused to EGFP in mammalian cells

   Depositor

   James Bamburg

   Article

   Garvalov et al J Neurosci. 2007 Nov 28;27(48):13117-29.

   Insert

   cofilin 1 (CFL1 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 5, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. CMV-Brainbow-1.1 M

    Plasmid

    #18722

    Depositor

    Joshua Sanes

    Article

    Livet et al Nature. 2007 Nov 1. 450(7166):56-62.

    Insert

    Kusabira ; M-mCherry ; M-mEYFP ; M-mCerulean
    

    Use

    Cre/Lox

    Expression

    Mammalian

    Available Since

    Aug. 15, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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11. pPB-TetON-mEGFP-MYOD1_AroPERFECT

    Plasmid

    #215620

    Purpose

    For integration of MYOD1 AroPERFECT T2A mEGFP

    Depositor

    Denes Hnisz

    Article

    Naderi et al Nat Cell Biol. 2024 Aug;26(8):1309-1321.

    Insert

    MYOD1 AroPERFECT (MYOD1 Human)
    

    Tags

    T2A mEGFP

    Expression

    Mammalian

    Available Since

    March 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. HA Nprl3 pRK5

    Plasmid

    #46330

    Depositor

    David Sabatini

    Article

    Bar-Peled et al Science. 2013 May 31;340(6136):1100-6. d

    Insert

    Nprl3 (NPRL3 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Available Since

    July 15, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBabe-tetoff-mVenus-PICdc42

    Plasmid

    #91876

    Purpose

    PI(WT): Photo inhibitable (blue light sensitive)

    Depositor

    Klaus Hahn

    Article

    Dagliyan et al Science. 2016 Dec 16;354(6318):1441-1444

    Insert

    Vav2 (VAV2 Human)
    

    Tags

    mVenus

    Expression

    Bacterial and Mammalian

    Available Since

    July 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. AAV-FLEX-FLIM-AKAR

    Plasmid

    #60445

    Purpose

    Expresses the Fluorescence Lifetime Imaging Microscopy (FLIM)-compatible PKA activity sensor FLIM-AKAR in a Cre dependent manner in an AAV backbone

    Depositor

    Bernardo Sabatini

    Article

    Chen et al Front Pharmacol. 2014 Apr 2;5:56. doi: 1

    Insert

    FLIM-AKAR
    

    Use

    AAV and Cre/Lox

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Oct. 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. 4X PRE TK luc

    Plasmid

    #206159

    Purpose

    Luciferase reporter construct containing 4 consensus PGR binding sites upstream of a minimal TK promoter, derived from Addgene #11350

    Depositor

    Renee van Amerongen

    Article

    Aarts et al J Mammary Gland Biol Neoplasia. 2023 Nov

    Insert

    4X PRE TK luciferase
    

    Use

    Luciferase

    Expression

    Mammalian

    Available Since

    Nov. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pTopo_Ex2Donor_pPGK-PURO

    Plasmid

    #198862

    Purpose

    Donor template for CRISPR/Cas9 targeting of TERT Ex2

    Depositor

    Agnel Sfeir

    Article

    Laprade et al Mol Cell. 2020 Jul 2;79(1):115-126.e6. d

    Insert

    PURO
    

    Expression

    Mammalian

    Promoter

    PGK

    Available Since

    March 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. SNAP-Dcp2

    Plasmid

    #153975

    Purpose

    SNAP-tag polypeptide fused to mRNA decapping enzyme 2

    Depositor

    Gia Voeltz

    Article

    Lee et al Science. 2020 Jan 31;367(6477). pii: 367

    Insert

    mRNA Decapping enzyme 2 (DCP2 Human)
    

    Tags

    SNAP-C1

    Expression

    Mammalian

    Available Since

    July 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. TFORF3305

    Plasmid

    #144781

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    ESR1 (ESR1 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Aug. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pHR-Tre3G-47xCAG-12xMS2

    Plasmid

    #99148

    Purpose

    Encodes 47xCAG repeats with 12xMS2 tag, 2nd generation lentiviral vector

    Depositor

    Ron Vale

    Article

    Jain et al Nature. 2017 Jun 8;546(7657):243-247. do

    Insert

    47xCAG 12xMS2 hairpins
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    TRE3G

    Available Since

    Aug. 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3.1 ZoAl-ENT N-1F (R1103A)

    Plasmid

    #216548

    Purpose

    For expression of N-flag tagged ZoAl-ENT R2 protein in mammalian cells

    Depositor

    Kathleen Collins

    Article

    Zhang et al Nat Biotechnol. 2024 Feb 20. doi: 10.103

    Insert

    R2 retroelement protein
    

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    March 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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