82,530 results
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Plasmid#83497PurposeExpression of a fluorescent granule marker, neuropeptide-Y, for localization and exocytosis studies.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertNPY (NPY Human)
Tagstandem mOrange2ExpressionMammalianMutationnonePromoterCMVAvailable sinceNov. 8, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3-MEK5DD-HA
Plasmid#65247PurposeExpression of HA tagged dominant active MEK5 in mammalian cellsDepositorInsertMEK5DD (MAP2K5 Human)
TagsHAExpressionMammalianMutationchanged Ser311 to Asp, Ser315 to AspPromoterCMVAvailable sinceNov. 7, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pRK5-EGFR
Plasmid#65225PurposeExpression of EGFR in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertEGFR (EGFR Human)
ExpressionMammalianPromoterCMVAvailable sinceNov. 7, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pp-mCAT1
Plasmid#83948PurposeThis plasmid co-expressed with VSVG allows the production of mCAT-1 gesicles rendering target cells permissive to transduction with Ecotropic lenti/retrovectorsDepositorAvailable sinceOct. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
RSV/hGATA3
Plasmid#83814PurposeMammalian expression of human GATA3DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertGATA3 (GATA3 Human)
ExpressionMammalianPromoterRSVAvailable sinceOct. 3, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3-ALK5 K232R
Plasmid#80878Purposemammalian expression of ALK5 K232RDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertALK5 (TGFBR1 Human)
TagsHAExpressionMammalianMutationK232R (kinase dead)PromoterCMVAvailable sinceAug. 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
CIB81-pmGFP
Plasmid#75366PurposeExpresses aa1-81 of Arabidopsis CIB1 fused to EGFP with CaaX membrane tag on endDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCIB1 (CIB1 Mustard Weed)
Tagsprenylated EGFPExpressionMammalianMutationresidues 1-81 of CIB1PromoterCMVAvailable sinceAug. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
vGAT-pH pcaggs SE
Plasmid#78578Purposeimage recycling of VGAT in live neuronsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertvGAT-pHluorin C-term (Slc32a1 Rat)
Tagssuperecliptic pHluorinExpressionMammalianPromoterchicken actinAvailable sinceJuly 13, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pYN4+
Plasmid#78715Purposemammalian expression of NHE1DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertNHE1 (SLC9A1 Human)
TagsHA3xExpressionMammalianMutationD720GPromoterRSV-LTRAvailable sinceJune 16, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCDNA3-HA-Akt1-K179M
Plasmid#73409PurposeExpresses kinase-inactive mutant of Akt with HA tagDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertAKT1 (AKT1 Human)
TagsHA tagExpressionMammalianMutationchanged K179M to make Kinase inactive mutantPromoterCMVAvailable sinceApril 20, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-CAG-fDIO-oG-WPRE-SV40pA
Plasmid#74291PurposepAAV vector to express oG (optimized Glycoprotein) in a Flp-dependent mannerDepositorInsertoG (optimized Glycoprotein)
UseAAVExpressionMammalianMutationchimeric glycoproteinPromoterCAGAvailable sinceApril 1, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Ntn1-Fc-His
Plasmid#72104PurposeExpresses the entire Netrin 1 protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.DepositorAvailable sinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
tetO-FUW-eGFP-RHOA-Q63L
Plasmid#73081PurposeLentiviral transfer vector for tet-inducible expression of EGFP tagged human RHOA-Q63LDepositorInsertRHOA-Q63L (RHOA Human)
UseLentiviralTagseGFPExpressionMammalianMutationQ63L Constitutively activeAvailable sinceFeb. 24, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pHLmMBP-6
Plasmid#72346PurposeMammalian expression of cytoplasmic mMBP-fused proteins with C-terminal 6His-tag/Strep-tag II/HA-tagDepositorTypeEmpty backboneTags6His-tag, HA-tag, Strep-tag II, and mMBPExpressionMammalianPromoterCMV enhancer + chicken beta-actin promoterAvailable sinceFeb. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
pmCherry-FKBP12-C1
Plasmid#67900PurposeExpresses a fusion of mCherry with FKBP12 in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertmCherry-FKBP12
UseSynthetic BiologyExpressionMammalianPromoterCMVAvailable sinceJan. 12, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Actin-mRFP-PAGFP
Plasmid#62382PurposeExpresses beta actin with both a PAGFP and mRFP tag.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertbeta actin (ACTB Human)
TagsPAGFP and mRFPExpressionMammalianPromoterCMVAvailable sinceNov. 12, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCMV/3' Box_(GLuc)_TOP1
Plasmid#68434PurposeTransient expression of the "TOP1" construct, targeting the GLuc reporter, in mammalian cells. CMV/3' Box expression backbone.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertTOP1 construct
UseCRISPR and Synthetic BiologyExpressionMammalianPromoterCMVAvailable sinceSept. 18, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
MC4R-Tango
Plasmid#66430PurposeExpression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocationDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertMC4R (MC4R Human)
TagsFLAGExpressionMammalianPromoterCMVAvailable sinceSept. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
AVPR2-Tango
Plasmid#66227PurposeExpression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocationDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertAVPR2 (AVPR2 Human)
TagsFLAGExpressionMammalianPromoterCMVAvailable sinceSept. 15, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
ADORA2A-Tango
Plasmid#66210PurposeExpression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocationDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertADORA2A (ADORA2A Human)
TagsFLAGExpressionMammalianPromoterCMVAvailable sinceSept. 15, 2015AvailabilityAcademic Institutions and Nonprofits only