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AAV-P(Per2)-DIO-intron2-dLUC

Plasmid

#110059

Purpose

Per2 transcription luciferase reporter

Depositor

Eric Erquan Zhang

Article

Mei et al Proc Natl Acad Sci U S A. 2018 Apr 2. pi

Insert

Per2 promoter and luciferase (Per2 Mouse)

Use

AAV and Cre/Lox

Expression

Mammalian

Promoter

Per2

Available Since

May 30, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-HA_human_BAP1

Plasmid

#158109

Purpose

Expresses tagged BAP1, neomycin selection

Depositor

Emanuela Felley-Bosco

Insert

BAP1 (BAP1 Human)

Expression

Mammalian

Available Since

Oct. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO_Lyn11-HaloTag9

Plasmid

#175543

Purpose

Expression of HaloTag9 at the inner leaflet of the plasma membrane of mammalian cells

Depositor

Kai Johnsson

Article

Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

Insert

Lyn11-HaloTag9

Expression

Mammalian

Mutation

HaloTag9 = HaloTag7-Q165H-P174R

Available Since

Dec. 13, 2021

Availability

Academic Institutions and Nonprofits only

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Stargazin-GFP-LOVpep

Plasmid

#80406

Purpose

Membrane localized GFP-tagged LOVpep. Expresses in mammalian cells

Depositor

Michael Glotzer

Article

Wagner et al J Cell Biol. 2016 Jun 20;213(6):641-9. d

Insert

Stargazin-GFP-LOVpep (CACNG2 Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 1, 2016

Availability

Academic Institutions and Nonprofits only

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Tagged-IRES

Plasmid

#35570

Depositor

Marian Waterman

Article

Tsai et al Mol Cell Proteomics. 2011 Apr;10(4):M110

Insert

Lymphoid Enhancer Factor 1 5'UTR (LEF1 Human)

Use

Luciferase

Tags

Firefly Luciferase and MS2 Stem Loop

Expression

Mammalian

Promoter

SV40

Available Since

April 3, 2012

Availability

Academic Institutions and Nonprofits only

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pDUAL CLDN (GFP)

Plasmid

#86981

Purpose

Lentiviral expression construct encoding Claudin-1 and GFP from separate promoters

Depositor

Joe Grove

Insert

CLDN1 (CLDN1 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

SFFV/hPGK

Available Since

Feb. 28, 2017

Availability

Academic Institutions and Nonprofits only

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AttB_ACE2-2A-TMPRSS2_IRES_iRFP670-H2A-P2A-PuroR

Plasmid

#190077

Purpose

express human ACE2 and human TMPRSS2 with selectable markers iRFP670 and puromycin resistance gene

Depositor

Kenneth Matreyek

Article

Roelle et al PLoS Biol. 2022 Jul 27;20(7):e3001738. d

Insert

ACE2 (ACE2, TMPRSS2 Human)

Expression

Mammalian

Promoter

none

Available Since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

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xCas9(3.7)-BE4

Plasmid

#108381

Purpose

Mammalian expression vector for xCas9(3.7)-BE4

Depositor

David Liu

Article

Hu et al Nature. 2018 Feb 28. pii: nature26155. d

Insert

xCas9(3.7)-BE4

Tags

NLS

Expression

Mammalian

Mutation

A262T, R324L, S409I, E480K, E543D, M694I, E1219V

Available Since

March 28, 2018

Availability

Academic Institutions and Nonprofits only

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pSicoR human Dicer1

Plasmid

#14763

Depositor

Tyler Jacks

Article

Kumar et al Nat Genet. 2007 May;39(5):673-7. doi: 10

Insert

Dicer shRNA (DICER1 Human)

Use

Cre/Lox, Lentiviral, and RNAi

Expression

Mammalian

Available Since

April 5, 2007

Availability

Academic Institutions and Nonprofits only

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FLAG-MST1

Plasmid

#1965

Depositor

Joseph Avruch

Article

Lin et al J Biol Chem 2002 Dec 13;277(50):47991-80

Insert

MST1 (Stk4 Mouse)

Tags

FLAG

Expression

Mammalian

Available Since

April 20, 2006

Availability

Academic Institutions and Nonprofits only

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GFP-Talin1

Plasmid

#26724

Depositor

Anna Huttenlocher

Article

Franco et al Nat Cell Biol. 2004 Oct . 6(10):977-83.

Insert

Talin (Tln1 Mouse)

Tags

EGFP

Expression

Mammalian

Available Since

Dec. 30, 2010

Availability

Academic Institutions and Nonprofits only

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TFORF1169

Plasmid

#142745

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

EBF1 (EBF1 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

June 8, 2023

Availability

Academic Institutions and Nonprofits only

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GLuc-FLAG-CB1

Plasmid

#158066

Purpose

Membrane expression detection of Cannabinoid receptor 1

Depositor

Adolfo Rivero-Muller

Article

Rodriguez-Rodriguez et al Sci Rep. 2020 Oct 23;10(1):18191. doi: 1

Insert

CB1 (CNR1 Human)

Use

AAV

Tags

Gaussia Luciferase

Expression

Mammalian

Mutation

contains 4Kb of 3' UTR

Promoter

pCAG

Available Since

Nov. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pmirGLO-3'UTR mouse Tnf

Plasmid

#207127

Purpose

Luciferase vector containing 3'UTR for mouse Tnf

Depositor

Silvia Monticelli

Article

Leoni et al Nat Commun. 2023 Jun 29;14(1):3862. doi:

Insert

Tumor necrosis factor (Tnf) 3'UTR (Tnf Mouse)

Use

Luciferase

Expression

Mammalian

Promoter

PGK promoter

Available Since

Dec. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pLVX-Puro-TDP-43-M337V

Plasmid

#133759

Purpose

Lentiviral expression of human TDP-43 M337V disease mutant in mammalian cells.

Depositor

Shawn Ferguson

Article

Roczniak-Ferguson et al Life Sci Alliance. 2019 Sep 16;2(5). pii

Insert

TDP-43 (TARDBP Human)

Use

Lentiviral

Expression

Mammalian

Mutation

Changed methionine 337 to valine

Promoter

CMV

Available Since

Nov. 25, 2019

Availability

Academic Institutions and Nonprofits only

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TFORF2815

Plasmid

#144244

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

FOSB (FOSB Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

April 27, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-SC35-cMyc

Plasmid

#44721

Depositor

Kathleen Scotto

Article

Shi et al Mol Cell Biol. 2008 Jan;28(2):883-95. Ep

Insert

SC35 (SRSF2 Human)

Tags

his and myc

Expression

Mammalian

Promoter

CMV

Available Since

April 29, 2013

Availability

Academic Institutions and Nonprofits only

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SPLICS Mt-ER- Po Long P2A

Plasmid

#164117

Purpose

Detect the long Peroxisomes-mitochondria-Endoplasmic reticulum contact

Depositor

Marisa Brini, Tito Calì

Article

Vallese et al Nat Commun. 2020 Nov 27;11(1):6069. doi:

Insert

Split GFP and YFP Mt-ER- Po Long P2A

Expression

Mammalian

Available Since

Feb. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Lyn-dInPAkt

Plasmid

#181834

Purpose

Genetically encoded single-color sensor for monitoring plasma membrane PI(3,4,5)P2 dynamics in living cells. Based on dimerization-dependent red fluorescent protein (ddRFP).

Depositor

Jin Zhang

Article

Hertel et al ACS Chem Biol. 2020 Jan 17;15(1):33-38.

Insert

Lyn-dInPAkt

Tags

N-terminal targeting sequence from Lyn kinase, dd…

Expression

Mammalian

Promoter

CMV

Available Since

June 17, 2022

Availability

Academic Institutions and Nonprofits only

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TFORF3195

Plasmid

#144671

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

ONECUT1 (ONECUT1 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

EF1a

Available Since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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