78,128 results

1. pEJS2700-CMV-Nme2-ABE8e-nt-E932R

   Plasmid

   #228721

   Purpose

   Expresses N-terminal ABE8e-Nme2 nCas9 (D16A) base editor with E932R mutation in mammalian cells

   Depositor

   Erik Sontheimer

   Article

   Bamidele et al bioRxiv November 07, 2024

   Insert

   N-terminal ABE8e with Nme2 nCas9 (D16A) E932R
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   Nme2-Cas9 D16A E932R

   Promoter

   CMV

   Available Since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. HRE-doUnaG

   Plasmid

   #201713

   Purpose

   Hypoxia responsive promoter driving UnaG fluorescent protein destablized using ODDD fragment from HIF1a and a PEST sequence

   Depositor

   Friedemann Kiefer

   Article

   Erapaneedi et al EMBO J. 2016 Jan 4;35(1):102-13. doi: 10

   Insert

   UnaG
   

   Expression

   Mammalian

   Available Since

   Nov. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pcDNA3.2/v5-DEST hGlut1

   Plasmid

   #18085

   Depositor

   Wolf Frommer

   Article

   Takanaga et al FASEB J. 2010 Aug . 24(8):2849-58.

   Insert

   Glut1 (SLC2A1 Human)
   

   Expression

   Mammalian

   Available Since

   Feb. 17, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pLV VIVIT-GFP

   Plasmid

   #188707

   Purpose

   Lentivirus transfer plasmid encoding VIVIT-GFP

   Depositor

   David Tobin

   Article

   Brewer et al Cell Rep. 2022 Dec 13;41(11):111817. doi

   Insert

   VIVIT-GFP
   

   Use

   Lentiviral

   Tags

   GFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pAS_4xU6-PylT M15 (UUA) FLAG-Mma PylRS

   Plasmid

   #154774

   Purpose

   plasmid with 4xMma PylT (M15 mutant, UUA anticodon) cassette and Mma PylRS for ochre suppression in transient or stable, piggybac-mediated, integration

   Depositor

   Simon Elsaesser

   Article

   Meineke et al Cell Rep. 2020 Jun 23;31(12):107811. doi

   Insert

   Mma PylRS (pylS Methanosarcina mazei)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   in PylT: UUA anticodon and G10A, G14U, G16U, C20U…

   Promoter

   EF1

   Available Since

   Oct. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. WT TXNIP

   Plasmid

   #200088

   Purpose

   Dox-inducible WT human TXNIP in pInducer20 vector

   Depositor

   Jason MacGurn

   Article

   Qualls-Histed et al iScience. 2023 Feb 6;26(3):106150. doi:

   Insert

   TXNIP (TXNIP Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. TFORF2364

   Plasmid

   #142051

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   CREB1 (CREB1 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   June 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pD649-HAsp-IL1R1-Fc(DAPA)-AviTag-6xHis

   Plasmid

   #156756

   Purpose

   Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.

   Depositor

   Chris Garcia

   Article

   Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

   Insert

   IL1R1 (IL1R1 Human)
   

   Tags

   Fc(DAPA)-AviTag-6xHis

   Expression

   Mammalian

   Mutation

   Fc region of human IgG contains D265A and P329A m…

   Promoter

   CMV/SP6

   Available Since

   March 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pBItetDT480GFP

   Plasmid

   #96905

   Purpose

   tet inducible bidirectional plasmid expressing GFP in one direction and in the other a human DMPK genomic segment containing exons 11-15 with 480 interrupted CTG repeats in exon 15

   Depositor

   Thomas Cooper

   Article

   Lee et al Proc Natl Acad Sci U S A. 2012 Mar 13;10

   Insert

   EGFP and human DMPK exons 11-15 with 480 interrupted CTG repeats (DMPK Human)
   

   Use

   Tetracycline responsive and bidirectional

   Expression

   Mammalian

   Promoter

   tetracycline responsive and bidirectional

   Available Since

   Sept. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAAV-EF1a-double floxed-ClopHensorN-WPRE-HGHpA

    Plasmid

    #193728

    Purpose

    Cre-activated AAV expression of ClopHensorN for measuring intracellular chloride and pH in genetically-defined cell types

    Depositor

    Colin Akerman

    Article

    Calin et al iScience. 2023 Mar 9;26(4):106363. doi:

    Insert

    ClopHensorN
    

    Use

    AAV

    Tags

    Strep-II (N terminal on insert)

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    March 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pBacMam2-DiEx-LIC-C-flag_huntingtin_full-length_Q54

    Plasmid

    #111727

    Purpose

    Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cells

    Depositor

    Cheryl Arrowsmith

    Article

    Harding et al J Biol Chem. 2019 Apr 26;294(17):6986-70

    Insert

    HTT (HTT Human)
    

    Use

    Baculovirus expression

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Q54 (polyQ repeat)

    Promoter

    CMV and P10

    Available Since

    Oct. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pLV-EBFP2-nuc

    Plasmid

    #36085

    Depositor

    Pantelis Tsoulfas

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    Replace EYFP of the vector pEYFP-Nuc from Clontec…

    Available Since

    June 1, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pLOX-gfp-iresTK

    Plasmid

    #12243

    Depositor

    Didier Trono

    Article

    Salmon et al Mol Ther. 2000 Oct . 2(4):404-14.

    Insert

    GFP, HSV1-TK
    

    Use

    Cre/Lox and Lentiviral

    Expression

    Mammalian

    Mutation

    LoxP in 3' LTR.

    Available Since

    Aug. 4, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. 1319 pcDNA3 flag FKHRL1 AAA

    Plasmid

    #10709

    Depositor

    William Sellers

    Article

    Ramaswamy et al Cancer Cell. 2002 Jul . 2(1):81-91.

    Insert

    FKHRL1 AAA (FOXO3 Human)
    

    Tags

    flag

    Expression

    Mammalian

    Mutation

    AAA mutant (see map) - T32A, S253A, S315A

    Available Since

    Nov. 2, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. RF-88-Ate1

    Plasmid

    #79187

    Purpose

    Expression of C-terminally Flag tagged ATE1 in mammalian cells

    Depositor

    Richard Fahlman

    Insert

    ATE1 (ATE1 Human)
    

    Tags

    3x Flag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. mCherry- eMagBF - 5ptaseOCRL

    Plasmid

    #162254

    Purpose

    Blue-light dependent heterodimerization system

    Depositor

    Pietro De Camilli

    Article

    Benedetti et al Elife. 2020 Nov 11;9. pii: 63230. doi: 1

    Insert

    mCherry- eMagBF - 5ptaseOCRL
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pLenti-C-STAT1-mGFP-P2A-Puro

    Plasmid

    #199345

    Purpose

    express murine STAT1 protein fused with mGFP

    Depositor

    Massimo Mallardo

    Article

    Caiazza et al Int J Mol Sci. 2022 Nov 17;23(22):14232.

    Insert

    signal transducer and activator of transcription 1 (Stat1 Mouse)
    

    Use

    Lentiviral

    Tags

    mGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pTubulin-Electra2

    Plasmid

    #184930

    Purpose

    Tubulin labeling with blue fluorescent protein Electra2

    Depositor

    Kiryl Piatkevich

    Article

    Papadaki et al Sci Rep. 2022 Jun 17;12(1):10190. doi: 1

    Insert

    TUBA1B-Electra2
    

    Tags

    Electra2

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. VCP(R155H)-EGFP

    Plasmid

    #23972

    Depositor

    Nico Dantuma

    Article

    Tresse et al Autophagy. 2010 Feb 22. 6(2):.

    Insert

    Valosin containing protein (VCP Human)
    

    Tags

    6xHis, EGFP, and HA

    Expression

    Mammalian

    Mutation

    R155H

    Available Since

    Feb. 26, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pNAHA

    Plasmid

    #44169

    Depositor

    John Olsen

    Article

    Patel et al J Gene Med. 2013 Jan;15(1):51-62. doi: 1

    Inserts

    hemagglutinin gene
    neuraminidase gene
    

    Expression

    Mammalian

    Mutation

    T55A

    Promoter

    CMV enhancer/human Ferritin light chain promoter …

    Available Since

    May 17, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge