78,128 results

1. pcDNA3-FKBP-EGFP-HOTag3

   Plasmid

   #106924

   Purpose

   Expresses FKBP-EGFP-HOTag3 in mammalian cells

   Depositor

   Xiaokun Shu

   Article

   Zhang et al Mol Cell. 2018 Jan 3. pii: S1097-2765(17

   Insert

   FKBP-EGFP-HOTag3
   

   Expression

   Mammalian

   Available Since

   March 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. mc2 mNeon

   Plasmid

   #206218

   Purpose

   For expressing cloned exons as circular RNA. Alternatively, for expressing cDNA (if the EcoRI-XhoI restriction fragment is also removed).

   Depositor

   Simon Conn, Brett Stringer

   Article

   Stringer et al bioRxiv. 2023 Nov 22:2023.11.21.568171.

   Type

   Empty backbone

   Use

   Synthetic Biology

   Expression

   Mammalian

   Available Since

   Aug. 31, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3-TrxRFP1

   Plasmid

   #98996

   Purpose

   mammalian expression of a biosensor to monitor thioredoxin redox dynamics

   Depositor

   Huiwang Ai

   Article

   Fan et al Nat Chem Biol. 2017 Jun 26. doi: 10.1038

   Insert

   human thioredoxin 1, GS-rich linker, fused with a redox-sensitive RFP
   

   Tags

   His-tag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNAintron-LASV-GPC

   Plasmid

   #215690

   Purpose

   Produces the Lassa virus glycoprotein

   Depositor

   Melinda Brindley

   Article

   Acciani et al J Virol. 2017 Aug 24;91(18):e00574-17. d

   Insert

   LASV GP
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 25, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. SARS-CoV2 Nucleocapsid

   Plasmid

   #153201

   Purpose

   Expresses myc-tagged nucleocapsid (N) from SARS-CoV2 in mammalian cells

   Depositor

   Peter Klein

   Article

   Liu et al Proc Natl Acad Sci U S A. 2021 Oct 19;11

   Insert

   Nucleocapsid from SARS-CoV-2 (N severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2))
   

   Tags

   Myc tag (5X)

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 15, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. R620-M12-303: CMV51p> SARS-CoV-2 S(1-1208)-2P-T4f-3C-His8-Strep2x2 B.1.351

   Plasmid

   #170196

   Purpose

   Mammalian expression of SARS-CoV-2 soluble spike trimer protein (B.1.351 variant)

   Depositor

   Dominic Esposito

   Article

   Esposito et al Protein Expr Purif. 2020 Jun 4;174:10568

   Insert

   SARS-CoV-2 S (spike) B.1.351 (S SARS-CoV-2)
   

   Tags

   3C-His8-Strep2x2

   Expression

   Mammalian

   Mutation

   L18F, D80A, D215G, Δ(242-244), R246I, K417N, E484…

   Promoter

   CMV51

   Available Since

   Nov. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. Integrin alpha L - mCFP

   Plasmid

   #8637

   Depositor

   Timothy Springer

   Article

   Kim et al Science 2003 Sep 19;301(5640):1720-5.

   Insert

   Integrin, alpha L (ITGAL Human)
   

   Tags

   CFP

   Expression

   Mammalian

   Available Since

   July 18, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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8. pWN_U6-TRAC-miniGag-Cas9

   Plasmid

   #228959

   Purpose

   miniEDV production plasmid. Expresses the miniGag-Cas9 polypeptide (CAG promoter) and sgRNA (human U6 promoter)

   Depositor

   Jennifer Doudna

   Article

   Ngo et al Proc Natl Acad Sci U S A. 2025 Jan 7;122

   Insert

   miniGag-Cas9
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Jan. 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. CDC40

   Plasmid

   #155444

   Purpose

   For use in RBP tethering screen

   Depositor

   Eugene Yeo

   Article

   Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

   Insert

   CDC40 (CDC40 Human)
   

   Tags

   V5-MCP

   Expression

   Mammalian

   Mutation

   none

   Promoter

   EF-1a

   Available Since

   Oct. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. LCV2 V-1

    Plasmid

    #84804

    Purpose

    Cas9 with sgRNA targeting Exon 1 of VEGF-A

    Depositor

    Glenn Yiu

    Article

    Yiu et al Invest Ophthalmol Vis Sci. 2016 Oct 1;57

    Insert

    gRNA targeting VEGF-A (VEGFA Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Nov. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. TGN38 EGFP

    Plasmid

    #128148

    Purpose

    Expression of TGN38 amino acids 304-357

    Depositor

    Jennifer Lippincott-Schwartz

    Insert

    TGN38 (Tgoln2 Rat)
    

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    Contains amino acids 304-357

    Available Since

    Nov. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCIneo-V5-SBP-MBP-HsStaufen1-T2_V

    Plasmid

    #147743

    Purpose

    Mammalian Expression of HsStaufen1-T2

    Depositor

    Elisa Izaurralde

    Insert

    HsStaufen1-T2 (STAU1 Human)
    

    Expression

    Mammalian

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. TFORF2998

    Plasmid

    #144474

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    GATA2 (GATA2 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    April 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLX304 Luciferase-V5 blast

    Plasmid

    #98580

    Purpose

    Lentiviral vector for constitutive expression of Luciferase with C-terminal V5 tag

    Depositor

    Kevin Janes

    Article

    Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

    Insert

    Luciferase
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Available Since

    Aug. 15, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA-wtATMSPARK2

    Plasmid

    #195247

    Purpose

    express wtATMSPARK2

    Depositor

    Xiaokun Shu

    Article

    Li et al Sci Adv. 2023 Mar;9(9):eade3760. doi: 10

    Insert

    ATMSPARK2 sensor
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. PB-5'-ENGRAM

    Plasmid

    #179157

    Purpose

    piggyBac plasmid for 5' ENGRAM recorder

    Depositor

    Jay Shendure

    Article

    Chen et al Nature. 2024 Aug;632(8027):1073-1081. do

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Expression

    Mammalian

    Promoter

    minP

    Available Since

    July 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. EGFP-BICD1

    Plasmid

    #49487

    Purpose

    expresses EGFP-BICD1 in mammalian cells

    Depositor

    Marci Scidmore

    Article

    Moorhead et al Infect Immun. 2007 Feb;75(2):781-91. Epu

    Insert

    BICD1 (BICD1 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    ** see notes

    Promoter

    CMV

    Available Since

    June 22, 2015

    Availability

    Academic Institutions and Nonprofits only

18. pSL3713 (pTnsC_Pse)

    Plasmid

    #200900

    Purpose

    Expresses human codon-optimized Pseudoalteromonas TnsC from a CMV promoter.

    Depositor

    Samuel H. Sternberg

    Article

    Lampe et al Nat Biotechnol. 2023 Mar 29. doi: 10.103

    Insert

    PseTnsC
    

    Tags

    3xNLS

    Expression

    Mammalian

    Available Since

    May 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLKO_FOXA1_#2

    Plasmid

    #70096

    Purpose

    Expression of shRNA to human FOXA1, puromycin selection

    Depositor

    William Hahn

    Article

    Pomerantz et al Nat Genet. 2015 Nov;47(11):1346-51. doi:

    Insert

    FOXA1 (FOXA1 Human)
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Promoter

    u6

    Available Since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLKO-perox-tagRFP

    Plasmid

    #185600

    Purpose

    Lentiviral construct for expression of tagRFP-T targeted to peroxisomes

    Depositor

    Jarrod Johnson

    Article

    Greiner et al Curr Protoc. 2022 Aug;2(8):e514. doi: 10

    Insert

    perox-tagRFP-T
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Aug. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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