78,128 results

1. pcDNA3_2FLAG-AKAP79 WT

   Plasmid

   #183450

   Purpose

   Expression of human wild-type AKAP79

   Depositor

   Matthew Gold

   Article

   Patel et al Nat Commun. 2017 Nov 22;8(1):1681. doi:

   Insert

   AKAP5 (AKAP5 Human)
   

   Tags

   2xFLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pRluc-LC3G120A

   Plasmid

   #105003

   Purpose

   Mammalian expression of Rluc-LC3G120A. Can be used for measuring autophagic flux.

   Depositor

   Marja Jããttelã

   Article

   Farkas et al Methods Enzymol. 2017;588:1-13. doi: 10.

   Insert

   LC3B (Map1lc3b Rat)
   

   Use

   Luciferase

   Tags

   Rluc with C124A substitution

   Expression

   Mammalian

   Mutation

   Glycine 120 mutated to alanine, prevents lipidati…

   Available Since

   Jan. 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pcDNA3-ApaLI*-HA

   Plasmid

   #211461

   Purpose

   Express mitochondrial localized ApaLI with D211A, E225A and K227A mutations

   Depositor

   Agnel Sfeir

   Article

   Fu et al Mol Cell. 2023 Oct 8:S1097-2765(23)00753

   Insert

   ApaLI mutant
   

   Expression

   Mammalian

   Mutation

   D211A, E225A, K227A

   Promoter

   CMV

   Available Since

   Jan. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pBa.Halo-LDLR

   Plasmid

   #162717

   Purpose

   Mammalian expression of human LDLR

   Depositor

   Marvin Bentley

   Article

   Frank et al Traffic. 2020 Sep 21. doi: 10.1111/tra.1

   Insert

   LDLR (LDLR Human)
   

   Tags

   Halo

   Expression

   Mammalian

   Mutation

   none

   Promoter

   Chicken Beta actin

   Available Since

   Dec. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pcDNA3.1-FRB-LgBiT

   Plasmid

   #182650

   Purpose

   FRB-LgBiT

   Depositor

   Masaharu Somiya

   Article

   Somiya et al Mol Pharm. 2022 May 20. doi: 10.1021/acs

   Insert

   FRB-LgBiT fusion
   

   Tags

   LgBiT

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. CF-Ins54p

   Plasmid

   #20155

   Depositor

   Tobias Meyer

   Article

   Suh et al Science. 2006 Dec 1. 314(5804):1454-7.

   Insert

   CFP-FKBP-Inp54p (INP54 Budding Yeast)
   

   Expression

   Mammalian

   Mutation

   Stop codon at position 331.

   Available Since

   March 24, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCMV6-AC 4-μHOM

   Plasmid

   #113619

   Purpose

   Reporter for Alt-EJ, variant of EJ7-GFP. Double strand breaks are induced with the 7a & 7h, or 7a & 7i sgRNAs, with CAS9. EJ using 4 nt of microhomology causes a deletion mutation and restores GFP

   Depositor

   Jeremy Stark

   Article

   Bhargava et al Nat Commun. 2018 Jun 27;9(1):2484. doi:

   Insert

   4-μHOM variant of EGFP
   

   Expression

   Mammalian

   Available Since

   Aug. 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pNUT N6His hTFNG

   Plasmid

   #67240

   Purpose

   Expresses N-His tagged nonglycosylated human serum transferrin in mammalian cells

   Depositor

   Ross MacGillivray, Anne Mason

   Article

   Mason et al Biochemistry. 2002 Jul 30;41(30):9448-54

   Insert

   human serum transferrin (TF Human)
   

   Tags

   N-terminal signal peptide, 4 aa link, 6 His, Fact…

   Expression

   Mammalian

   Mutation

   Asn413 Asp, Asn611Asp

   Promoter

   SV40

   Available Since

   Sept. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. HA-Rab11-DN (S25N)

   Plasmid

   #101046

   Purpose

   Expresses HA-tagged human Rab11-DN (S25N) in mammalian cells

   Depositor

   Ken-Ichi Takemaru

   Article

   Burke et al J Cell Biol. 2014 Oct 13;207(1):123-37.

   Insert

   RAB11 (RAB11A Human)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   S25N

   Promoter

   CMV

   Available Since

   Sept. 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pmTurquoise2-Tubulin

    Plasmid

    #36202

    Purpose

    In vivo visualization of microtubules (can be used for colocalization studies)

    Depositor

    Dorus Gadella

    Article

    Goedhart et al Nat Commun. 2012 Mar 20;3:751. doi: 10.1

    Insert

    Alpha-tubulin (TUBA1B Human)
    

    Tags

    mTurquoise2

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 15, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. GFP-UBR5 ∆HECT

    Plasmid

    #52051

    Purpose

    CMV driven mammalian expression of ligase-dead UBR5 with an N-termianl GFP fusion

    Depositor

    Darren Saunders

    Article

    Gudjonsson et al Cell. 2012 Aug 17;150(4):697-709. doi: 1

    Insert

    UBR5 ∆HECT (UBR5 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    C2769A

    Promoter

    CMV

    Available Since

    March 24, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pcDNA3.1-PDGFRB-V5/HIS

    Plasmid

    #201988

    Purpose

    expression of human PDGFRB receptor tyrosine kinase in mammalian cells

    Depositor

    Pavel Krejčí

    Article

    Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

    Insert

    PDGFRB receptor tyrosine kinase (PDGFRB Human)
    

    Tags

    V5, 6xHis

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. MSCV PIG (Puro IRES GFP)

    Plasmid

    #18751

    Depositor

    Scott Lowe

    Type

    Empty backbone

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    July 3, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pEGFP-H1.5

    Plasmid

    #32898

    Depositor

    Michael Hendzel

    Article

    Th'ng et al J Biol Chem. 2005 Jul 29;280(30):27809-1

    Insert

    Histone H1.5 (H1-5 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 19, 2018

    Availability

    Academic Institutions and Nonprofits only

15. iNos-eGFP

    Plasmid

    #207251

    Purpose

    Reporter for expression of eGFP under control of iNos promoter

    Depositor

    Michelle Farkas

    Article

    Mas-Rosario et al Front Oncol. 2023 Apr 5;13:1151384. doi:

    Insert

    iNos Promoter (Nos2 Mouse)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    July 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCMV-Klb-EGFP

    Plasmid

    #45531

    Depositor

    Zipora Yablonka-Reuveni

    Article

    Phelps et al FEBS J. 2016 May;283(9):1653-68. doi: 10

    Insert

    beta Klotho (Klb Mouse)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 18, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. HaloTag-A2A-adenosine receptor

    Plasmid

    #66995

    Purpose

    A2A-adenosine receptor tagged with HaloTag on amino terminus

    Depositor

    Catherine Berlot

    Insert

    HaloTag-A2A-adenosine receptor (ADORA2A Human)
    

    Tags

    Human Growth hormone Signal Sequence- HaloTag

    Expression

    Mammalian

    Mutation

    The signal sequence from human growth hormone was…

    Promoter

    CMV

    Available Since

    July 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pQLL-MAG-5 kb

    Plasmid

    #218305

    Purpose

    Donor plasmid of MAG Transposon with 5 kb cargo gene

    Depositor

    Haoyi Wang

    Article

    Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

    Insert

    5 kb cargo gene
    

    Expression

    Mammalian

    Available Since

    Oct. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. FU- dio PSD95-mCherry- W

    Plasmid

    #73919

    Purpose

    Expresses PSD-95 tagged with mCherry. Expression is Cre dependent. Plasmid can also be used to make lentivirus.

    Depositor

    Elly Nedivi

    Article

    Villa et al Neuron. 2016 Feb 3. pii: S0896-6273(16)0

    Insert

    PSD-95-mCherry
    

    Use

    Lentiviral

    Tags

    mCherry and myc

    Expression

    Mammalian

    Promoter

    Ubi-C

    Available Since

    April 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. mRuby2-Lysosomes-20

    Plasmid

    #55902

    Purpose

    Localization: Lysosome Membrane, Excitation: 559, Emission: 600

    Depositor

    Michael Davidson

    Article

    Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

    Insert

    LAMP1
    

    Tags

    mRuby2

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 10, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge