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aTY171_pPGK1_iRFP_WPRE

Plasmid

#225351

Purpose

Lentiviral vector for iRFP expression

Depositor

Wendell Lim

Article

Yamada et al Cell. 2024 Dec 11:S0092-8674(24)01323-0.

Insert

iRFP670

Use

Lentiviral

Expression

Mammalian

Available Since

Dec. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pD649-HAsp-SIRPB1.3-COMP5AP-AviTag-9xHis

Plasmid

#157346

Purpose

Mammalian expression of cell-surface protein extracellular domain (isoform 3) fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

SIRPB1 (SIRPB1 Human)

Tags

COMP5AP-AviTag-9xHis

Expression

Mammalian

Promoter

CMV/SP6

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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ACE2-IgFc probe

Plasmid

#184787

Purpose

Expression of His-tagged, soluble ACE2-IgFc microbody

Depositor

Ajit Chande

Article

Mishra et al Front Cell Infect Microbiol. 2021 Apr 23

Insert

hACE2-IgFc (ACE2 Synthetic)

Tags

6xHis Tag

Expression

Mammalian

Promoter

EF1-alpha

Available Since

Oct. 19, 2022

Availability

Academic Institutions and Nonprofits only

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CeNL(Ca2+)_110μ-pcDNA3

Plasmid

#111929

Purpose

Cyan color bright luminescent calcium indicator with low affinity

Depositor

Takeharu Nagai

Article

Hossain et al ACS Chem Biol. 2018 Mar 9. doi: 10.1021/

Insert

CeNL(Ca2+)_110μ

Expression

Mammalian

Mutation

E31D, F92W, E104D. D133E at CaM

Promoter

CMV

Available Since

June 29, 2018

Availability

Academic Institutions and Nonprofits only

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dCas9-DNMT3a-DNMT3l-3xFLAG-tagBFP

Plasmid

#154139

Purpose

Expresses the dCas9-DNMT3a-DNMT3l-3xFLAG-tagBFP fusion protein for targeted DNA methylation.

Depositor

Albert Jeltsch

Article

Hofacker et al Int J Mol Sci. 2020 Jan 13;21(2). pii: i

Insert

dCas9, DNMT3a (catalytic domain), DNMT3l (C-terminal part), tagBFP

Tags

3x FLAG and tagBFP

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 20, 2021

Availability

Academic Institutions and Nonprofits only

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Plenti-ARE-HSVTK-luc

Plasmid

#161789

Purpose

Expresses HSV-TK and Luciferase in an NRF2 dependent manner. Also, expresses cherry and blasticidin resistance selection markers

Depositor

Hans-Guido Wendel

Article

Sanghvi et al Cell. 2019 Aug 8;178(4):807-819.e21. doi

Inserts

Luciferase-T2A-HSV-TK
mCherry P2A blasticidin

Use

Lentiviral and Luciferase

Expression

Mammalian

Promoter

EF1a and Murine Gclc ARE

Available Since

Feb. 10, 2021

Availability

Academic Institutions and Nonprofits only

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VPS13A^Halo

Plasmid

#118759

Purpose

VPS13A internally tagged with Halo at residue 1372.

Depositor

Pietro De Camilli

Article

Kumar et al J Cell Biol. 2018 Oct 1;217(10):3625-363

Insert

VPS13A (VPS13A Human)

Tags

Halo tag inserted at position 1372

Expression

Mammalian

Available Since

Jan. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pLKO.1-puro-shNT

Plasmid

#109012

Purpose

short-hairpin knockdown of targeted gene

Depositor

Jacob Corn

Article

Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

Insert

Non-targeting shRNA

Use

Lentiviral

Expression

Mammalian

Promoter

hU6 promoter

Available Since

June 20, 2018

Availability

Academic Institutions and Nonprofits only

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pAK005

Plasmid

#233642

Purpose

For bacterial expression of eSpCas9(1.1) c-terminally tagged with VitelloTagB.

Depositor

Zak Swartz

Article

Clarke et al Development. 2024 Oct 15;151(20):dev2028

Insert

eSpCas9(1.1) with c-terminal VitelloTagB

Tags

6-His

Expression

Mammalian

Available Since

April 15, 2025

Availability

Academic Institutions and Nonprofits only

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negMaLionR

Plasmid

#113909

Purpose

negative control for Red ATP sensor

Depositor

Tetsuya Kitaguchi

Article

Arai et al Angew Chem Int Ed Engl. 2018 Jun 27. doi

Insert

negMaLionR

Expression

Mammalian

Available Since

March 26, 2019

Availability

Academic Institutions and Nonprofits only

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MIP 227 CoMiP 4in1 wo shRNA p53

Plasmid

#63727

Purpose

To express Oct4, Klf4, Sox2, and c-Myc. A single plasmid reprogramming system using a mini-intronic plasmid (MIP).

Depositor

Mark Kay, Joseph Wu

Article

Diecke et al Sci Rep. 2015 Jan 28;5:8081. doi: 10.103

Insert

Mini-intronic-plasmid intron (POU5F1, KLF4, SOX2, MYC Human)

Tags

IRES-dtomato

Expression

Mammalian

Mutation

codon-optimised

Promoter

SFFV

Available Since

Aug. 28, 2015

Availability

Academic Institutions and Nonprofits only

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AAV-GfaABC1D-ERCreER-4x6T

Plasmid

#196411

Purpose

Astrocytic expression of ERT2-Cre-ERT2 recombinase in AAV; astrocyte specificity enhanced with 4x6T miRNA targeting cassette

Depositor

Stanley Thomas Carmichael

Article

Gleichman et al Nat Commun. 2023 Nov 16;14(1):7426. doi:

Insert

ERT2-Cre-ERT2

Use

AAV; Astrocyte-selective

Expression

Mammalian

Promoter

CAG and GfaABC1D

Available Since

Feb. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pPB-cT3G-cERP2-DMC1

Plasmid

#222541

Purpose

PiggyBac transposon plasmid for doxycycline inducible expression of DMC1

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2024.05.31.596483

Insert

DMC1 (DMC1 Human)

Tags

HA

Expression

Mammalian

Available Since

Oct. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR221_SLC45A4

Plasmid

#132179

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC45A4 (SLC45A4 Human)

Expression

Mammalian

Available Since

Oct. 25, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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mTq2-Calmodulin

Plasmid

#198200

Purpose

FRET donor: Calmodulin-2 tagged with N-terminal mTurquoise2

Depositor

Christian Wahl-Schott

Article

Feldmann et al STAR Protoc. 2023 Jul 28;4(3):102459. do

Insert

Calmodulin 2 (Calm2 Rat)

Tags

mTurquoise2

Expression

Mammalian

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pMX hCD4

Plasmid

#14614

Depositor

Dan Littman

Article

Landau et al Nature. 1988 Jul 14. 334(6178):159-62.

Insert

CD4 (CD4 Human)

Use

Retroviral

Expression

Mammalian

Available Since

June 27, 2007

Availability

Academic Institutions and Nonprofits only

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hPGK_V5-APEX2-GFP

Plasmid

#105285

Purpose

drives expression of V5-NES-APEX2-GFP from human PGK promotor

Depositor

Eugene Yeo

Article

Markmiller et al Cell. 2018 Jan 25;172(3):590-604.e13. do

Insert

V5-APEX2-NES

Tags

GFP

Expression

Mammalian

Promoter

human PGK promoter

Available Since

Oct. 25, 2018

Availability

Academic Institutions and Nonprofits only

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MultiMate 5 colors

Plasmid

#206266

Purpose

Expression of 5 fluorescently labelled proteins in mammalian cells. Can be used as a CRE-donor (MultiBac system)

Depositor

Imre Berger

Article

Aulicino et al Nucleic Acids Res. 2022 Jul 22;50(13):77

Insert

H2B (H. Sapiens), Actin and Tubulin (B. taurus), mito mCherry (Synthetic), CyOFP1 (E. quadricolor)

Use

Recombinant baculovirus production, cre acceptor…

Expression

Mammalian

Available Since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-UBF

Plasmid

#26672

Depositor

Sui Huang

Article

Chen et al J Cell Biol. 2001 Apr 2. 153(1):169-76.

Insert

UBF1 (UBTF Human)

Tags

EGFP

Expression

Mammalian

Available Since

Nov. 10, 2010

Availability

Academic Institutions and Nonprofits only
pEYFP-C1-Drp1

Plasmid

#45160

Depositor

Richard Youle

Article

Frank et al Dev Cell. 2001 Oct;1(4):515-25.

Insert

Drp1 (DNM1L Human)

Tags

EYFP

Expression

Mammalian

Promoter

CMV

Available Since

May 22, 2013

Availability

Academic Institutions and Nonprofits only

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