78,131 results

1. human EB1 fused to monomeric RFP

   Plasmid

   #39323

   Depositor

   Tim Mitchison, Jennifer Tirnauer

   Inserts

   EB1 (MAPRE1 Human)
   mRFP
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 30, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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2. Tet-O-FUW-ZEB2-P2A-mCherry

   Plasmid

   #203857

   Purpose

   Tet-inducible lentiviral plasmid expressing ZEB2-P2A-mCherry, with ORF specific barcode close to 3'LTR site

   Depositor

   Andrea Califano

   Article

   Obradovic et al Cancer Cell. 2023 May 8;41(5):933-949.e1

   Insert

   ZEB2 (ZEB2 Human)
   

   Expression

   Mammalian

   Available Since

   Aug. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. HDM_VSV_G

   Plasmid

   #204156

   Purpose

   VSV-G expression plasmid

   Depositor

   Jesse Bloom

   Article

   Dadonaite et al Cell. 2023 Mar 16;186(6):1263-1278.e20.

   Insert

   VSV-G
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1-EBFP2(1-10)

   Plasmid

   #165141

   Purpose

   Expresses EBFP2(1-10) in mammalian cells

   Depositor

   Daichi Kamiyama

   Article

   Tamura et al Commun Biol. 2021 Feb 26;4(1):257. doi:

   Insert

   EBFP2(1-10)
   

   Expression

   Mammalian

   Available Since

   April 8, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. HA-Rab11-WT

   Plasmid

   #101047

   Purpose

   Expresses HA-tagged human Rab11-WT in mammalian cells

   Depositor

   Ken-Ichi Takemaru

   Article

   Burke et al J Cell Biol. 2014 Oct 13;207(1):123-37.

   Insert

   RAB11 (RAB11A Human)
   

   Tags

   HA

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDRM166 LKB (Luciferase mKate2 Blast)

   Plasmid

   #183502

   Purpose

   Expresses the firefly luciferase gene, the NLS-tagged mKate2 gene, and the blasticidin resistance gene joined by P2A linkers.

   Depositor

   Wayne Tilley

   Article

   Mustafa et al Oncogene. 2023 Nov 24. doi: 10.1038/s413

   Inserts

   Firefly Luciferase
   mKate2
   BSD
   

   Use

   Lentiviral and Luciferase

   Tags

   P2A linker and SV40 NLS with GGS linker

   Expression

   Mammalian

   Promoter

   MND, MND (off Luc-P2A), and MND (off Luc-P2A-mKat…

   Available Since

   Nov. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. 3XFlagNICD3

   Plasmid

   #20185

   Depositor

   Raphael Kopan

   Article

   Ong et al J Biol Chem. 2006 Feb 24. 281(8):5106-19

   Insert

   Notch 3 Intracellular Domain (Notch3 Mouse)
   

   Tags

   3X Flag

   Expression

   Mammalian

   Mutation

   Contains murine Notch 3 Intracellular Domain (Met…

   Promoter

   CMV

   Available Since

   Dec. 13, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLAMP1-miRFP670nano

   Plasmid

   #127435

   Purpose

   LAMP1 (Lysosome Membrane) labeling with near-infrared fluorescent protein miRFP670nano

   Depositor

   Vladislav Verkhusha

   Article

   Oliinyk et al Nat Commun. 2019 Jan 17;10(1):279. doi:

   Insert

   LAMP1-miRFP670nano
   

   Tags

   miRFP670nano

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. paGFP-Omp25

   Plasmid

   #69598

   Purpose

   expresses GFP-labeled mitochondrial outer membrane protein 25

   Depositor

   David Sabatini

   Article

   Katajisto et al Science. 2015 Apr 17;348(6232):340-3. do

   Insert

   paGFP-Omp25 (Synj2bp Rat)
   

   Use

   Lentiviral

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   photoactivatable GFP fused to rat Omp25 C-terminus

   Promoter

   CMV

   Available Since

   Dec. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. MAC_C_AOX

    Plasmid

    #111661

    Purpose

    MAC-tagged gene expression

    Depositor

    Markku Varjosalo

    Article

    Liu et al Nat Commun. 2018 Mar 22;9(1):1188. doi:

    Insert

    AOX
    

    Expression

    Mammalian

    Available Since

    June 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. 3xFLAG-dCas9/pMXs-neo

    Plasmid

    #51260

    Purpose

    Expresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions of interest.

    Depositor

    Hodaka Fujii

    Article

    Fujita et al PLoS One. 2014 Jul 22;9(7):e103084. doi:

    Insert

    3xFLAG-dCas9
    

    Use

    CRISPR and Retroviral

    Tags

    3xFLAG tag and NLS (nuclear localization signal)

    Expression

    Mammalian

    Mutation

    human codon-optimized, D10A + H840A

    Promoter

    LTR

    Available Since

    Feb. 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCMV-T7-ABE8e-SpCas9-NRRH-P2A-EGFP (RMD51)

    Plasmid

    #197502

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized ABE8e A-to-G base editor with nSpCas9-NRRH(D10A) and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

    Insert

    pCMV-T7-BPNLS-ABE8e-nSpCas9-NRRH-BPNLS-P2A-EGFP
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS and BPNLS-P2A-EGFP

    Expression

    Mammalian

    Mutation

    ABE8e mutations in TadA and nSpCas9-NRRH(D10A/I32…

    Promoter

    CMV and T7

    Available Since

    March 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLX_TRC311 ETV1

    Plasmid

    #74981

    Purpose

    Constitutive ETV1 ORF, blasticidin selection

    Depositor

    William Hahn

    Article

    Wang et al Cell Rep. 2017 Feb 7;18(6):1543-1557. do

    Insert

    ETV1 (ETV1 Human)
    

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    May 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. PB-TET

    Plasmid

    #20909

    Purpose

    piggyBac empty vector with Gateway cassette, tet inducible promoter, and linked reporter (IRES-bgeo)

    Depositor

    Andras Nagy

    Article

    Woltjen et al Nature. 2009 Apr 9;458(7239):766-70. doi

    Insert

    Gateway Destination Vector
    

    Use

    piggybac

    Tags

    IRES-bGeo

    Expression

    Mammalian

    Available Since

    May 5, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCMV-mCherry-MHC-IIA

    Plasmid

    #35687

    Depositor

    Venkaiah Betapudi

    Article

    Dulyaninova et al Mol Biol Cell. 2007 Aug;18(8):3144-55. E

    Insert

    MHC IIA (MYH9 Human)
    

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 24, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCF140_Gag-SpyCas9-NLS

    Plasmid

    #225959

    Purpose

    CMV-Intron-Gag-SpyCas9-NLS. Expresses Gag-SpyCas9-NLS for VLP production.

    Depositor

    Christof Fellmann

    Article

    Ramadoss et al bioRxiv [Preprint]. 2024 Jun 26:2024.06.

    Insert

    CMV-Intron-Gag-SpyCas9-NLS
    

    Expression

    Mammalian

    Available Since

    Oct. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCMV-SEMPER_ACC-msfGFP[r5M]_ACC-mEBFP2_IRES-mCherry

    Plasmid

    #221075

    Purpose

    SEMPER plasmid for expression of msfGFP[r5M] and mEBFP2 in ratios dictated by the trinucleotide TIS. Relative ORF expression is characterized in publication

    Depositor

    Mikhail Shapiro

    Article

    Duan et al Cell Syst. 2024 Jul 17;15(7):597-609.e4.

    Insert

    ACC-msfGFP[r5M]_ACC-mEBFP2_IRES-mCherry
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pEGFPC1-muscle Amphiphysin 2

    Plasmid

    #22213

    Depositor

    Pietro De Camilli

    Article

    Butler et al J Cell Biol. 1997 Jun 16. 137(6):1355-67

    Insert

    Amphiphysin II (BIN1 Human)
    

    Tags

    GFP

    Expression

    Bacterial and Mammalian

    Available Since

    Oct. 9, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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19. c-myc promoter (Del 4)

    Plasmid

    #16604

    Depositor

    Bert Vogelstein

    Article

    He et al Science. 1998 Sep 4. 281(5382):1509-12.

    Insert

    c-myc promoter (Del 4) (MYC Human)
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Mutation

    Del-4 (PvuII->PvuII). The first PvuII site is …

    Available Since

    March 24, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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20. MLRV2: NF-kb-SMAD-DBE-P53-AP1

    Plasmid

    #124536

    Purpose

    Multiplex luciferase reporter vector, with luciferase reporters for the pathways NFKB, SMAD, FOXO, P53 and AP1

    Depositor

    Koen Venken

    Article

    Sarrion-Perdigones et al Curr Protoc Mol Biol. 2020 Jun;131(1):e1

    Inserts

    5 copies of the NF-kb DNA binding motif
    7xSMAD_RE::FLuc
    3xDBE_BE::Renilla (FOXO)
    2xP53_RE::NLuc
    6xAP-1_RE::GrRenilla
    

    Use

    Luciferase and Synthetic Biology

    Expression

    Mammalian

    Available Since

    June 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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