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82,530 results

Showing: 10461 - 10480 of 82530 results

  9. HA-BirA*-pDEST-N-pcDNA5/FRT/TO

    Plasmid
    #118375
    Purpose
    Expression vector for BioID in mammalian system. Gateway compatible destination vector to generate stable, inducible cell line expressing N-terminally tagged HABirA* and protein of interest.
    Depositor
    Maria Vartiainen
    Publication
    Viita et al J Cell Sci. 2019 Apr 17;132(8). pii: jcs
    Type
    Empty backbone
    Use
    Destination
    Tags
    HA-BirA*
    Expression
    Mammalian
    Available since
    Nov. 30, 2018
    Availability
    Academic Institutions and Nonprofits only
    118375-plasmid-map-sequence-id-229581

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  11. CMV-CASANOVA

    Plasmid
    #113035
    Purpose
    CASANOVA (AcrIIA4-LOV2 hybrid) expression in mammalian cells; enables optogenetic control of SpyCas9
    Depositor
    Dominik Niopek
    Publication
    Bubeck et al Nat Methods. 2018 Oct 30. pii: 10.1038/s
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CASANOVA (for CRISPR/Cas9 activity switching via a novel optogenetic variant of AcrIIA4)
    Use
    CRISPR and Synthetic Biology
    Expression
    Mammalian
    Available since
    Oct. 31, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    113035-plasmid-map-sequence-id-224728

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  12. PH-PLCD1_mScarlet_IRES_SYFP2_PH_N1

    Plasmid
    #85069
    Purpose
    Yellow/red FRET biosensor for monotoring plasmamembrane PtdIns(4,5)P2 levels (PIP2)
    Depositor
    Dorus Gadella
    Publication
    Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    PH-PLCD1 (PLCD1 Human)
    Tags
    IRES-sYFP2-PH and mScarlet
    Expression
    Mammalian
    Mutation
    PLCD aa 1–170
    Promoter
    CMV
    Available since
    Oct. 2, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    85069-plasmid-map-sequence-id-269155

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  15. AICSDP-64: FUS-mEGFP

    Plasmid
    #114410
    Purpose
    Homology arms and linker-mEGFP sequence for C-terminus tagging of human FUS
    Depositor
    Allen Institute for Cell Science
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    FUS Homology Arms with linker-mEGFP (FUS Human)
    Use
    CRISPR; Donor template
    Tags
    linker-mEGFP
    Expression
    Mammalian
    Mutation
    homology arms contain point mutations to disrupt …
    Available since
    Sept. 5, 2018
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    114410-plasmid-map-sequence-id-220875

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  19. pLenti-xBE4Gam-P2A-Puro

    Plasmid
    #112676
    Purpose
    Lentivirus for constitutive expression of xCas9(3.7)-BE4Gam in mammalian cells (codon optimized)
    Depositor
    Lukas Dow
    Publication
    Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419
    Insert
    xCas9(3.7)-BE4Gam
    Use
    Lentiviral
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    D10A, A262T, R324L, S409I, E480K, E543D, M694I, E…
    Promoter
    EF1s
    Available since
    July 30, 2018
    Availability
    Academic Institutions and Nonprofits only
    112676-plasmid-map-sequence-id-217196

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Showing: 10461 - 10480 of 82530 results