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pDONR221-SLC4A8_STOP

Plasmid

#161466

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC4A8 (SLC4A8 Human)

Expression

Mammalian

Available Since

Sept. 15, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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Double UP mClover3 to mScarlet

Plasmid

#120262

Purpose

Natively produces mClover3, in the presence of Cre will be recombined to produce mScarlet.

Depositor

Erik Dent

Article

Taylor et al Front Mol Neurosci. 2020 May 20;13:82. d

Insert

mClover3/mScarletP2A-mcs

Expression

Mammalian

Mutation

Removed Not1 site in mScarlet

Promoter

CAG

Available Since

March 19, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV-Tag4A-MITF-M (L135V)

Plasmid

#31154

Depositor

Yardena Samuels

Article

Cronin et al Pigment Cell Melanoma Res. 2009 Aug . 22

Insert

MITF-M (MITF Human)

Tags

FLAG

Expression

Bacterial and Mammalian

Mutation

Leucine 135 changed to Valine

Available Since

Sept. 13, 2011

Availability

Academic Institutions and Nonprofits only
pCMV-Tag4A-MITF-M (E87R)

Plasmid

#31152

Depositor

Yardena Samuels

Article

Cronin et al Pigment Cell Melanoma Res. 2009 Aug . 22

Insert

MITF-M (MITF Human)

Tags

FLAG

Expression

Bacterial and Mammalian

Mutation

Glutamic acid 87 changed to Arginine

Available Since

Sept. 13, 2011

Availability

Academic Institutions and Nonprofits only
mCherry-Clathrin LC-15

Plasmid

#55019

Purpose

Localization: Clathrin Vesicles, Excitation: 587, Emission: 610

Depositor

Michael Davidson

Article

Rizzo et al Cold Spring Harb Protoc. 2009 Dec;2009(1

Insert

Clathrin LC (CLTB Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 29, 2014

Availability

Academic Institutions and Nonprofits only

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pDA056

Plasmid

#131387

Purpose

ORFeus expression, WT

Depositor

Kathleen Burns

Article

Ardeljan et al Nat Struct Mol Biol. 2020 Feb;27(2):168-

Insert

ORFeus-Hs

Expression

Mammalian

Promoter

TRE3G

Available Since

June 8, 2020

Availability

Academic Institutions and Nonprofits only

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pY116 (pcDNA3.1-huMb2Cpf1)

Plasmid

#92292

Purpose

Expression of humanized Mb2Cpf1

Depositor

Feng Zhang

Insert

huMb2Cpf1

Tags

NLS and NLS-3xHA

Expression

Mammalian

Available Since

June 22, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-TFEBS122D-MYC (#777)

Plasmid

#99956

Purpose

mammalian expression

Depositor

James Brugarolas

Article

Vega-Rubin-de-Celis et al Autophagy. 2017 Mar 4;13(3):464-472. doi

Insert

TFEB (TFEB Human)

Tags

6xHis and Myc

Expression

Mammalian

Mutation

S122D

Available Since

Nov. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pSG5-BALF5

Plasmid

#72630

Purpose

Mammalian expression vector for Epstein-Barr virus DNA polymerase

Depositor

S. Diane Hayward

Article

Sarisky et al J Virol. 1996 Dec;70(12):8340-7.

Insert

BALF5

Expression

Mammalian

Mutation

G249S

Available Since

Feb. 11, 2016

Availability

Academic Institutions and Nonprofits only
pcDNA6 asyn E46K

Plasmid

#105730

Purpose

Mammalian expression of mutant human alpha synuclein

Depositor

Hilal Lashuel

Article

Mbefo et al J Biol Chem. 2015 Apr 10;290(15):9412-27

Insert

alpha-synuclein E46K (SNCA Human)

Expression

Mammalian

Mutation

E46K

Promoter

CMV

Available Since

March 29, 2018

Availability

Academic Institutions and Nonprofits only

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pATZ-BFP-HA-GFP11

Plasmid

#177721

Purpose

ER protein alpha-1-antitrypsin containing E342K mutation (ATZ mutant) fused to blue fluorescence protein (BFP) tag protein, an HA tag and the eleventh β-strand of GFP

Depositor

Erik Böttger

Article

Cortes Sanchon et al Commun Biol. 2021 Dec 2;4(1):1350. doi:

Insert

alpha-1-antitrypsin (SERPINA1 Human)

Tags

GFP11, HA, and TagBFP

Expression

Bacterial and Mammalian

Mutation

E342K (ATZ mutant)

Available Since

Jan. 26, 2022

Availability

Academic Institutions and Nonprofits only

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p-mCherry2-sgRNA (empty)

Plasmid

#198330

Purpose

Customisable sgRNA sequence with optimised sgRNA scaffold for expression under U6 promoter, with mCherry2 reporter

Depositor

Sarah McClelland

Article

Tovini et al EMBO J. 2023 May 15;42(10):e111587. doi:

Insert

U6-sgRNA(F+E) empty

Expression

Mammalian

Promoter

U6

Available Since

April 14, 2023

Availability

Academic Institutions and Nonprofits only

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pDARMO_CMVT_N_Strep_BIRC6

Plasmid

#197972

Purpose

Mammalian cell expression of wt Strep-BIRC6

Depositor

Eric Fischer

Article

Hunkeler et al Science. 2023 Mar 17;379(6637):1105-1111

Insert

BIRC6 (BIRC6 Human)

Tags

Strep

Expression

Mammalian

Mutation

L1332V

Available Since

April 19, 2023

Availability

Academic Institutions and Nonprofits only

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pCDNA5/mCherry_pBirA-HA_FKBP8

Plasmid

#154457

Purpose

expresses mCherry_pBirA-HA_FKBP8/biochemical assay

Depositor

Hyun-Woo Rhee

Article

Kwak et al Proc Natl Acad Sci U S A. 2020 May 15. p

Insert

mCherry_pBirA-HA_FKBP8 (FKBP8 Human)

Expression

Mammalian

Promoter

CMV-F

Available Since

Aug. 24, 2020

Availability

Academic Institutions and Nonprofits only

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C1A-C1A-EYFP

Plasmid

#61155

Purpose

Diacylglycerol (DAG) reporter

Depositor

Tobias Meyer

Article

Tsai et al Nat Cell Biol. 2014 Feb;16(2):133-44. do

Insert

C1A-C1A-EYFP, C1A from PKCG (aa 26-89, rat) (Prkcg Rat)

Tags

EYFP

Expression

Mammalian

Mutation

C1A from PKCG amino acids 26-89

Promoter

CMV

Available Since

Dec. 2, 2014

Availability

Academic Institutions and Nonprofits only

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NanoLuc-KRAS(G12D)_CRAF(FL)-HaloTag BiBRET Vector

Plasmid

#238579

Purpose

Express NanoLuc-KRAS(G12D)_CRAF(FL)-HaloTag Fusion Protein in Mammalian Cells under a CMV promoter

Depositor

Promega Corporation

Has Service

DNA

Inserts

KRAS (KRAS Human)
CRAF (RAF1 Human)

Tags

HaloTag (R) and NanoLuc (R)

Expression

Mammalian

Mutation

G12D

Promoter

CMV

Available Since

Sept. 14, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pDisplay-GRAB_ATP1.0mut-IRES-mCherry-CAAX

Plasmid

#167583

Purpose

Express the ATP-insensitive control sensor GRAB_ATP1.0mut in mammalian cells

Depositor

Yulong Li

Article

Wu et al Neuron. 2021 Dec 17. pii: S0896-6273(21)

Insert

ATP-insensitive sensor GRAB_ATP1.0mut (control sensor) (P2RY1 Human)

Expression

Mammalian

Promoter

CMV

Available Since

April 21, 2021

Availability

Academic Institutions and Nonprofits only

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pLEX307 GFP-Kif26b-C

Plasmid

#118483

Purpose

lentiviral expression of GFP-Kif26b-C in mammalian cells

Depositor

Henry Ho

Article

Karuna et al Genes (Basel). 2018 Apr 5;9(4). pii: gen

Insert

mouse Kif26b-C (Kif26b Mouse)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

Encodes GFP fused to amino acid 1735 to 2112 of m…

Promoter

EF1A

Available Since

March 10, 2020

Availability

Academic Institutions and Nonprofits only

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BICstim-Gag-dCAS9-VPR

Plasmid

#120922

Purpose

encodes a GAG-dCAS9-VPR fusion for targeted transcriptional activation.

Depositor

Philippe Mangeot, Théophile Ohlmann, Emiliano Ricci

Article

Mangeot et al Nat Commun. 2019 Jan 3;10(1):45. doi: 10

Insert

GAG (FMLV)-dCAS9-VPR

Tags

none

Expression

Mammalian

Promoter

hCMV

Available Since

April 9, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3-ClopHensor

Plasmid

#25938

Depositor

Daniele Arosio

Article

Arosio et al Nat Methods. 2010 Jul . 7(7):516-8.

Insert

ClopHensor

Tags

Strep-II

Expression

Mammalian

Mutation

E2GFP is EGFP-T203Y

Available Since

Sept. 13, 2010

Availability

Academic Institutions and Nonprofits only

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