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82,585 results

Showing: 11981 - 12000 of 82585 results

  1. pCMV-SaPE2-P2A-BSD

    Plasmid
    #174818
    Purpose
    Mammalian expression of SaCas9 prime editor 2 with P2A-BSD marker
    Depositor
    David Liu
    Publication
    Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SaPE2-P2A-BSD
    Tags
    SV40 bpNLS
    Expression
    Mammalian
    Mutation
    Detailed in manuscript
    Promoter
    CMV
    Available since
    Oct. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    174818-plasmid-map-sequence-id-343609

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  2. pAB2076 pAAV REPAIR.t1

    Plasmid
    #176323
    Purpose
    pAAV EFS-HIVNES-dCas13bt1- (GGS)2-huADAR2(E488Q)-3xHA BGHpolyA::U6-BpiI-Cas13bt1 DR (full REPAIR.t1 + crRNA expression for AAV production)
    Depositor
    Feng Zhang
    Publication
    Kannan et al Nat Biotechnol. 2021 Aug 30. pii: 10.103
    Inserts
    human codon optimized Cas13bt1 (catalytically inactivated)
    huADAR2dd(E488Q) (ADARB1 Human)
    Cas13bt1 crRNA + BpiI cloning site
    Use
    AAV and CRISPR
    Tags
    3xHA and HIV NES
    Expression
    Mammalian
    Mutation
    E488Q, only the deaminase domain (aa 276-702 are …
    Promoter
    EFS (short EF1alpha) and hU6
    Available since
    Oct. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    176323-plasmid-map-sequence-id-345286

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  4. pCMV-Csy4-PE2

    Plasmid
    #172667
    Purpose
    Enhanced prime editing system in mammalian cells. This plasmid is used by PE3 and PE3b
    Depositor
    Xiaolong Wang
    Publication
    Liu et al Cell Res. 2021 Jun 8. pii: 10.1038/s4142
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Csy4-PE2
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Aug. 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    172667-plasmid-map-sequence-id-339511

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  6. CMV-VPR-S-L1-dCjCas9 MiniCAFE

    Plasmid
    #169910
    Purpose
    Expression of a truncated VPR-dCjCas9 fusion protein to activate gene expression.
    Depositor
    Zhili Rong
    Publication
    Zhang et al Nucleic Acids Res. 2021 Mar 22. pii: 617
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    MiniCAFE
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    D8A, HNH-truncation (Δ495–609 aa) in CjCas9
    Promoter
    CMV
    Available since
    July 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    169910-plasmid-map-sequence-id-336045

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  7. pCMV-RdLight1

    Plasmid
    #125706
    Purpose
    Expresses the red genetically encoded dopamine sensor RdLight1 in mammalian cells.
    Depositor
    Lin Tian
    Publication
    Patriarchi et al Nat Methods. 2020 Nov;17(11):1147-1155.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    RdLight1
    Tags
    Flag tag
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    July 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    125706-plasmid-map-sequence-id-246158

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  8. pCMVd1-NLS-GAL4-iLight-VP16-T2A-mTagBFP2

    Plasmid
    #170273
    Purpose
    NLS-Gal4(1-65)-iLight(human codon optimized)-VP16-T2A-mTagBFP2 construct expression in mammalian cells.
    Depositor
    Vladislav Verkhusha
    Publication
    Kaberniuk et al Nat Commun. 2021 Jun 23;12(1):3859. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    NLS-Gal4(1-65)-iLight(human codon optimized)-VP16-T2A-mTagBFP2
    Expression
    Mammalian
    Mutation
    See Comments
    Promoter
    CMVd1
    Available since
    June 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    170273-plasmid-map-sequence-id-334945

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  10. GFP-PIK3C2B

    Plasmid
    #161989
    Purpose
    Mammalian expression vector containing PIK3C2B tagged with GFP
    Depositor
    David Bryant, Volker Haucke
    Publication
    Marat et al Science. 2017 Jun 2;356(6341):968-972. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    PIK3C2B (PIK3C2B Human)
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    siRNA resistance to siRNA#2 with 4 silent mutatio…
    Available since
    June 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    161989-plasmid-map-sequence-id-333558

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  11. LiPOP2

    Plasmid
    #168266
    Purpose
    A single-component optogenetic tool for photoswitchable pyroptosis
    Depositor
    Yubin Zhou
    Publication
    He et al Adv Sci (Weinh). 2021 May 6;8(13):210042
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mGSDMD(1-276) (Gsdmd Mouse)
    Tags
    cpLOV2, mCherry
    Expression
    Mammalian
    Mutation
    R138A, K146A, R152A, R154A
    Promoter
    CMV
    Available since
    June 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    168266-plasmid-map-sequence-id-334500

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  16. nLuc-GLP-1R-His-FLAG

    Plasmid
    #124831
    Purpose
    Expresses N-terminally NanoLuc-Tagged GLP-1R in mammalian cells
    Depositor
    Sam Gellman
    Publication
    Cary et al Nat Chem Biol. 2022 Mar;18(3):256-263. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    GLP1R (GLP1R Human, Synthetic)
    Use
    Luciferase
    Tags
    FLAG, His6, and NanoLuc
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    April 12, 2021
    Availability
    Academic Institutions and Nonprofits only
    124831-plasmid-map-sequence-id-251141

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Showing: 11981 - 12000 of 82585 results