79,901 results

Showing: 1941 - 1960 of 79901 results

Results per page:

Sort results by:

pTSHZ2.1.0-gDNA

Plasmid

#113778

Purpose

CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor TSHZ2

Depositor

Kevin White

Insert

TSHZ2 (TSHZ2 Human)

Use

CRISPR

Expression

Mammalian

Available Since

Dec. 11, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pSpCas9n(BB)-2A-GFP (PX461)

Plasmid

#48140

Purpose

Cas9n (D10A nickase mutant) from S. pyogenes with 2A-EGFP, and cloning backbone for sgRNA

Depositor

Feng Zhang

Article

Ran et al Nat Protoc. 2013 Nov;8(11):2281-308. doi

Insert

hSpCas9n

Use

CRISPR

Tags

3XFLAG and GFP

Expression

Mammalian

Mutation

Cas9 D10A nickase mutant

Promoter

Cbh

Available Since

Oct. 29, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
HA-HIF1alpha-pcDNA3

Plasmid

#18949

Depositor

William Kaelin

Article

Kondo et al Cancer Cell. 2002 Apr . 1(3):237-46.

Insert

Hypoxia inducible factor 1 alpha (HIF1A Human)

Tags

HA-tag

Expression

Mammalian

Mutation

wt cDNA

Available Since

Sept. 3, 2008

Availability

Academic Institutions and Nonprofits only

Enlarge
pLYS1-FLAG-MitoGFP-HA

Plasmid

#50057

Purpose

Expression of GFP targeted to mitochondria with Flag and HA tags

Depositor

Vamsi Mootha

Article

Sancak et al Science. 2013 Nov 14.

Insert

Mito-GFP

Use

Lentiviral

Tags

Flag and HA

Expression

Mammalian

Available Since

Jan. 13, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
HyPer7.2DAAO-NLS

Plasmid

#168302

Purpose

Chemogenetic fluorescent H2O2 reporter targeted to the nucleus

Depositor

Thomas Michel

Article

Saeedi Saravi et al Redox Biol. 2020 Sep;36:101605. doi: 10.

Insert

HyPer7.2DAAO-NLS

Expression

Mammalian

Promoter

CMV

Available Since

May 4, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
MSCV Puro

Plasmid

#68469

Purpose

Retroviral plasmid with multiple cloning site and puromycin resistance

Depositor

Tyler Jacks

Article

Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

Insert

Puromycin Resistance

Use

Retroviral

Expression

Mammalian

Promoter

pGK

Available Since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
MCS-BioID2-HA

Plasmid

#74224

Purpose

To fuse your protein of interest to the N-terminus of BioID2 and use in proximity-dependent biotin identification (BioID); MCS is present upstream of BioID2 tag and HA tag

Depositor

Kyle Roux

Article

Kim et al Mol Biol Cell. 2016 Feb 24. pii: mbc.E15

Has Service

Cloning Grade DNA

Type

Empty backbone

Tags

HA and humanized A. aeolicus BioID2 with R40G

Expression

Mammalian

Promoter

CMV

Available Since

April 1, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
LV-hGFAP-GFP

Plasmid

#183906

Purpose

Expression of GFP under the human GFAP ABC1D promoter

Depositor

Chun-Li Zhang

Article

Niu et al Nat Cell Biol. 2013 Oct;15(10):1164-75.

Insert

GFP (GFAP Human)

Use

Lentiviral

Expression

Mammalian

Available Since

May 19, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pLV6-mPER2-luc

Plasmid

#212035

Purpose

Expression of murine Per2-luciferase in mammalian cells

Depositor

David Hazlerigg

Article

Appenroth et al J Biol Rhythms. 2024 Dec;39(6):554-567.

Insert

mPer2-luciferase (Per2 Mouse)

Use

Lentiviral and Luciferase

Tags

luciferase

Expression

Mammalian

Available Since

Dec. 20, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pHR-iCasp9

Plasmid

#199441

Purpose

Express inducible Caspase9 in mammalian cells

Depositor

Chao Tang

Article

Yuan et al Cell Death Dis. 2022 Jan 10;13(1):34. do

Insert

inducible Caspase9 (CASP9 Human)

Use

Lentiviral

Tags

DmrB and mCherry

Expression

Bacterial and Mammalian

Available Since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
Flag-Keap1

Plasmid

#28023

Depositor

Qing Zhong

Article

Fan et al Autophagy. 2010 Jul 22. 6(5):.

Insert

Keap1 (KEAP1 Human)

Tags

3xFlag

Expression

Mammalian

Available Since

Feb. 17, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
All-in-one lenti-evoCDA-NG

Plasmid

#200449

Purpose

Lentivirus vector for base editing

Depositor

Benjamin Cravatt

Article

Li et al Nat Chem Biol. 2023 Nov;19(11):1320-1330

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

June 14, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV(MinDis).iGluSnFR

Plasmid

#41732

Purpose

Single-wavelength extracellular glutamate sensor constructed from E. coli Gltl and cpGFP. Membrane-displayed. ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

Depositor

Loren Looger

Article

Marvin et al Nat Methods. 2013 Feb;10(2):162-70. doi:

Insert

GltI based Glutamate Biosensor

Use

Glutamate biosensor

Tags

Myc, PDGFR transmembrane helix (aa 513-561), and …

Expression

Mammalian

Mutation

cpGFP146 variant from EcMBP165-cpGFP.PPYF.pRSET i…

Promoter

CMV

Available Since

Jan. 3, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pmGFP-P2A-K(AAA)20-P2A-RFP

Plasmid

#105688

Purpose

Double fluorescent stalling reporter K(AAA)20

Depositor

Ramanujan Hegde

Article

Juszkiewicz et al Mol Cell. 2017 Feb 16;65(4):743-750.e4.

Inserts

P2A-3xFLAG-VHPbeta
K(AAA)20
P2A-mCherry

Expression

Mammalian

Promoter

CMV (from vector)

Available Since

Feb. 6, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
phage UbiC G3BP1-GFP-GFP

Plasmid

#119950

Purpose

Lentiviral vector expressing G3BP1-GFP fusion protein

Depositor

Jeffrey Chao

Article

Wilbertz et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

Insert

G3BP1 (G3BP1 Human)

Use

Lentiviral

Tags

GFP

Expression

Bacterial and Mammalian

Promoter

UbiC

Available Since

March 20, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
dCas9 plasmid

Plasmid

#100091

Purpose

dCas9 expression plasmid without effector fusions; 3X Flag tag; 2NLS; pCDNA3 vector backbone, mammalian expression

Depositor

David Segal

Article

O'Geen et al Nucleic Acids Res. 2017 Sep 29;45(17):99

Type

Empty backbone

Use

CRISPR

Tags

3XFlag-NLS-dCas9-NLS

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 30, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti PGK FKBP12(F36V)-OGT (Neo)

Plasmid

#154294

Purpose

Lentiviral vector encoding FKBP12(F36V)-2xHA-OGT (Neomycin resistant) off PGK promoter

Depositor

Suzanne Walker

Article

Levine et al Proc Natl Acad Sci U S A. 2021 Jan 26;11

Insert

O-GlcNAc Transferase (OGT Human)

Use

Lentiviral

Tags

2x HA and FKBP12(F36V)

Expression

Mammalian

Promoter

PGK

Available Since

Nov. 16, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pLVpuro-CMV-N-mCherry

Plasmid

#123221

Purpose

N-terminal mCherry tag. Gateway destination vector for lentivirus production and stable transduction in mammalian cells.

Depositor

Robin Ketteler

Article

Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

Type

Empty backbone

Use

Lentiviral; Gateway destination

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 18, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
SPLICS LY-MT Short P2A

Plasmid

#213614

Purpose

Detect the short-range Lysosome-Mitochondria contact

Depositor

Tito Calì

Article

Giamogante et al Nat Commun. 2024 Feb 19;15(1):1516. doi:

Insert

SPLICS LY-MT Short P2A

Expression

Mammalian

Available Since

Feb. 20, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pU6-tmpknot-GG-acceptor

Plasmid

#174039

Purpose

Prime editing in mammalian cells

Depositor

David Liu

Article

Nelson et al Nat Biotechnol. 2021 Oct 4. pii: 10.1038

Type

Empty backbone

Expression

Mammalian

Promoter

hU6

Available Since

Oct. 4, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge