4,307 results
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Plasmid#131860PurposeTransient expression of firefly luciferase from the Zea mays ubiquitin promotorDepositorInsertluciferase
UseTagsExpressionPlantMutationPromoterZmUBQAvailable sinceOct. 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDGB1alpha11_SulR
Plasmid#186424Purposetranscriptional unit for sulfadiazine resistance; plant expression driven by the Pnos promoter; suitable for quintuple assemblyDepositorInsertSulR
UseSynthetic Biology; Binary vector for escherichia …TagsExpressionPlantMutationBsaI and BsmBI sites removedPromoterPnosAvailable sinceJan. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
SunTagng14aa
Plasmid#106439PurposeEncodes a SunTag CRISPR cas9 system that does not contain a guide RNA and therefore, does not target the TET1 catalytic domain to any specific region of the genome.DepositorInsertNOS_TET1CD_2xNLS_1xHA__sfGFP_scFv_UBQ10_INSULATOR_UBQ10_dCAS9_1xHA_3xNLS_10xGCN414aa_OCS
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceMarch 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCBL101gw
Plasmid#199725PurposeA pVS1-based T-DNA binary vector and destination vector for Gateway cloning with NPTII as a plant selectable marker gene.DepositorInsertattR1/R2 cassette
UseTagsExpressionPlantMutationPromoterAvailable sinceJune 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGD283
Plasmid#129733PurposeDestination plasmid containing the 35S:XVE estradiol inducible driver and the A-G overhangs for GreenGate cloning of an expression casette for estradiole-inducible expression in plants.DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceOct. 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGrDL_SPb
Plasmid#83205Purposereceives miRNA target sequences for testing using the dual luciferase assay systemDepositorInsertTomato ACTIN promoter
UseTagsExpressionPlantMutationPromoterTomato ACTINAvailable sinceNov. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pYLCRISPR/Cas9P35S-B
Plasmid#66190Purposeexpression of Cas9 in plants, cauliflower mosaic virus 35S promoter (P35S), basta selectionDepositorInsertCas9
UseTagsExpressionPlantMutationplant-codon optimized with higher GC contents (62…Promotercauliflower mosaic virus 35S promoter (P35S)Available sinceOct. 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFGC5941-PacI-ath-STTM165/166-48nt
Plasmid#84091Purposetarget miRNA165/166 for destruction in Arabidopsis thalianaDepositorInsertSTTM165/166
UseTagsExpressionPlantMutationPromoter2x35SAvailable sinceNov. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pH-mono-MAD7
Plasmid#170134PurposeFor plant prime editing in rice plants or monocotyledons protoplastsDepositorInsertMAD7(ErCas12a)
UseCRISPRTagsExpressionPlantMutationPromotermaize Ubiquitin-1, switchgrass Ubiquitin-1Available sinceSept. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMU-TPr
Plasmid#61189PurposeFluorescent reporter for tonoplast AtTIP1-1-mCherry expressed under AtUBQ10 promoterDepositorInsertAtTIP1-1
UseTagsmCherryExpressionPlantMutationPromoterAtUBQ10Available sinceFeb. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pJJB302
Plasmid#107694PurposeOsActin Promoter-LwaCas13a-HSP TerminatorDepositorInsertCas13a
UseTagsExpressionPlantMutationPromoterOsActinAvailable sinceAug. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGEL850
Plasmid#214250PurposeExpression TadCBEa in rice cellsDepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceMay 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFH14
Plasmid#125799PurposeLevel0 SaCas9, Arabidopsis codon optimizedDepositorInsertSaCas9, Arabidopsis codon optimized
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMKV058
Plasmid#133313PurposeBeYDV viral replicon on T-DNA backbone expressing Firefly Luc+ and WUS2DepositorInsertLuc+. WUS2
UseTagsExpressionPlantMutationPromoterAtUbi10, NosAvailable sinceDec. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCambia-loxKan
Plasmid#199590Purposebasic plant vector with a plant-selectable kanamycin resistance cassette flanked by both loxP and FRT sites for sustainable plant engineering with subsequent marker removal possibilityDepositorInsertFRT-lox66-NosP-KanR-NosT-lox71-FRT
UseCre/LoxTagsExpressionPlantMutationPromoterNosAvailable sinceJuly 5, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGWB444
Plasmid#74830PurposeGateway cloning compatible binary vector for C-terminal fusion with eCFP (CaMV35S promoter).DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterCaMV35SAvailable sinceJuly 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBUE411-GG
Plasmid#198232PurposeWheat GRF4-GIF1 chimera in pBUE411DepositorInsertGRF4-GIF1 chimera cassette
UseCRISPR; Plant binary vectorTagsExpressionPlantMutationPromoterUbiquitinAvailable sinceJune 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSKI074
Plasmid#11571DepositorInsertCaMV 35S enhancers
UseActivation taggingTagsExpressionPlantMutationPromoterAvailable sinceMay 24, 2006AvailabilityAcademic Institutions and Nonprofits only -
pGWB544
Plasmid#74878PurposeGateway cloning compatible binary vector for C-terminal fusion with eCFP (CaMV35S promoter).DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterCaMV35SAvailable sinceJuly 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEGB 35S:Renilla:Tnos (GB0109)
Plasmid#68215PurposeTranscriptional unit for Renilla Luciferase Protein plant expression driven by the 35S promoterDepositorInsertRenilla
UseLuciferase and Synthetic BiologyTagsExpressionPlantMutationBsaI and BsmBI sites removedPromoter35SAvailable sinceOct. 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
EK0397 3FLAG-ADAR1-p150-HIS (pEAQ)
Plasmid#191172PurposeHuman ADAR1, p150 isoform in plant-expressable format.DepositorInsert3FLAG-ADAR1-p150-HIS (plant) (ADAR Human)
UseTags3FLAG and HISExpressionPlantMutationWTPromoterAvailable sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLaNGo11-7
Plasmid#126055PurposeA self-assembling split-GFP based Golden Gate vector to determine protein targeting to plastids in plant cellDepositorInsertschloroplastic FNR transit peptide
ccdB cassette (BsaI)
GFP11x7 (5 aa linker)
UseBinaryTagsGFP1-10ExpressionPlantMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHdzCas9-KRAB
Plasmid#92341PurposeExpression dzCas9-KRAB in microalgae. CRISPR/Cas based plant genome editing and gene regulation, Hyg resistanceDepositorTypeEmpty backboneUseCRISPR; Plant expressionTagsKRABExpressionPlantMutationPromoterCaMV35SAvailable sinceJune 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFGC5941-PacI-ath-STTM156/157
Plasmid#84083Purposetarget miRNA156/157 for destruction in Arabidopsis thalianaDepositorInsertSTTM156/157
UseTagsExpressionPlantMutationPromoter2x35SAvailable sinceJan. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJY-RpABE
Plasmid#112872Purposebinary vector for ABE expression in ArabidopsisDepositorInsertsRPS5A promoter
ABE7.10
U6-sgRNA cassette
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceSept. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
ePPEmax*
Plasmid#205244PurposeFor plant prime editing in wheat plants or monocotyledons protoplastsDepositorInsertc-myc NLS-bpNLS-nCas9-NC-MLV-vbpNLS-SV40NLS
UseCRISPRTagsExpressionPlantMutationDetailed in manuscriptPromoterAvailable sinceFeb. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYPQ-ABE-Cas9n-Act3.0
Plasmid#178956PurposeIt consists of a Cas9 nickase (D10A) fused with an adenine deaminase (ABE8e) and MS2-SunTag-activators (ScFv-sfGFP-2xTAD), enabling simultaneous A to G conversion and gene activation.DepositorInsertABE8e-zCas9-T2A-MS2-SunTag-rbcS-E9t-ter-ZmUbi-scFv-sfGFP-2xTAD-GB1-NOS-ter
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceMay 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUC19/mChe
Plasmid#183161PurposeConstruct for transient expression of cell nucleus-, cytoplasm-targeted mCherry in plants.DepositorInsertmCherry
UseTagsExpressionPlantMutationPromoterAvailable sinceAug. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFGC5941-PacI-ath-STTM160
Plasmid#84085Purposetarget miRNA160 for destruction in Arabidopsis thalianaDepositorInsertSTTM160
UseTagsExpressionPlantMutationPromoter2x35SAvailable sinceMarch 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAGM55273
Plasmid#153211PurposeLevel 2 cloning vector for a single guide RNA, already contains Cas9, Kanamycin selection cassette for plant transformation and FAST markerDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMC-6-BeYDV-Rep-RepA-9
Plasmid#197715PurposePhytobrick (MoClo) Level 0 PartDepositorInsertBeYDV Rep/RepA proteins: native ORFs
UseTagsExpressionPlantMutationPromoterAvailable sinceMay 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGWB513
Plasmid#74855PurposeGateway cloning compatible binary vector for C-terminal fusion with 3xHA (no promoter).DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceJuly 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFGC5941DR5-PacI-ath-STTM164
Plasmid#84090Purposetarget miRNA164 for destruction in Arabidopsis thalianaDepositorInsertSTTM164
UseTagsExpressionPlantMutationPromoter2x35SAvailable sinceDec. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFGC5941-PacI-ath-STTM319
Plasmid#84115Purposetarget miRNA319 for destruction in Arabidopsis thalianaDepositorInsertSTTM319
UseTagsExpressionPlantMutationPromoter2x35SAvailable sinceMarch 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pICSL11059
Plasmid#68263PurposeLevel 1 Golden Gate Cassette: Plant hygromycin (with intron) resistance cassetteDepositorInsertPromoter:35s + 5'UTR:Omega + CDS:hptIIwintron + 3'UTR/terminator:35s
UseSynthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceAug. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCMU-APr
Plasmid#61182PurposeFluorescent reporter for apoplast MtBCP1sp-mCherry expressed under AtUBQ10 promoterDepositorInsertMtBCPsp-mCherry
UseTagsmCherryExpressionPlantMutationPromoterAtUBQ10Available sinceMarch 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCAMBIA-FLS2-myc-mCherry
Plasmid#86159PurposeFLS2-mCherry expressionDepositorInsertFLS2 (FLS2 Mustard Weed)
UseTagsmCherry and mycExpressionPlantMutationPromoterAvailable sinceFeb. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
ePPE-V223A
Plasmid#205243PurposeFor plant prime editing in wheat plants or monocotyledons protoplastsDepositorInsertSV40_NLS-nCas9-NC-MLV-SV40_NLS
UseCRISPRTagsExpressionPlantMutationDetailed in manuscriptPromoterAvailable sinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAG1guide_RUBY_EggCas9
Plasmid#225983PurposeTo make mutant alleles of AtAGAMOUS1 gene in Arabidopsis thaliana. The transgenics can be selected by red color appearance of plantsDepositorInsertGuide RNA against AtAGAMOUS1
UseCRISPRTagsExpressionPlantMutationPromoter35s promoter to Drive RUBY and DD45p to drive Cas9Available sinceOct. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDIRECT_25F
Plasmid#91143PurposeDirect Cloning, Type: T-DNA, Engineering Reagent: ZmUbi:TaCas9 + TaU6:gRNA, Plant Selection: PvUbi2:hpt IIDepositorInsertEngineering Reagent: ZmUbi:TaCas9 + TaU6:gRNA
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceJuly 13, 2017AvailabilityAcademic Institutions and Nonprofits only