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4,625 results

Showing: 521 - 560 of 4625 results

  1. CMPE-ePPEplus

    Plasmid
    #205242
    Purpose
    For multiplex plant prime editing in wheat plants or monocotyledons protoplasts
    Depositor
    Zong Yuan
    Article
    Ni et al Genome Biol. 2023 Jun 29;24(1):156. doi:
    Insert
    Csy4-c-myc NLS-bpNLS-nCas9-NC-MLV-vbpNLS-SV40NLS
    Use
    CRISPR
    Expression
    Plant
    Mutation
    Detailed in manuscript
    Available Since
    Oct. 18, 2023
    Availability
    Academic Institutions and Nonprofits only
    205242-plasmid-map-sequence-id-408832

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  4. pH-nCas9-PPE-V2

    Plasmid
    #170131
    Purpose
    For plant prime editing in rice plants or monocotyledons protoplasts
    Depositor
    Caixia Gao
    Article
    Lin et al Nat Biotechnol. 2021 Mar 25. pii: 10.103
    Insert
    nCas9(H840A)-M-MLV
    Use
    CRISPR
    Expression
    Plant
    Mutation
    H840A for Cas9; D200N, T306K, W313F, T330P and L…
    Promoter
    maize Ubiquitin-1, OsU3
    Available Since
    May 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    170131-plasmid-map-sequence-id-333607

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  6. pPPED

    Plasmid
    #162468
    Purpose
    Destination Expression vector for dicot plant prime editing
    Depositor
    Adam Bogdanove
    Article
    Wang et al Front Genome Ed. 2021 Mar 4;3:617553. do
    Inserts
    Cas9H840A
    attR gateway destination cassette
    Tags
    MLV reverse transcriptase
    Expression
    Plant
    Mutation
    Change histidine 840 to Alanine based sequence o…
    Promoter
    2x35S
    Available Since
    Dec. 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    162468-plasmid-map-sequence-id-350748

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  11. pISV-DsRed1

    Plasmid
    #171024
    Purpose
    Agrobacterium rhizogenes-mediated hairy root transformation of Lotus japonicus.
    Depositor
    Christian Staehelin
    Type
    Empty backbone
    Expression
    Plant
    Promoter
    2X EN 35S
    Available Since
    Aug. 5, 2021
    Availability
    Academic Institutions and Nonprofits only
    171024-plasmid-map-sequence-id-337720

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  16. pCBC-MT2T3

    Plasmid
    #50594
    Purpose
    CRISPR/Cas based plant genome editing and gene regulation; used as template for making expression cassette with multiple gRNA target sites
    Depositor
    Qi-Jun Chen
    Article
    Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.
    Insert
    T2, gRNA scaffold, TaU3t plus, U6-26p, T3
    Use
    CRISPR; Pcr template
    Expression
    Plant
    Available Since
    Dec. 11, 2014
    Availability
    Academic Institutions and Nonprofits only
    50594-plasmid-map-sequence-id-75674

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  17. pBUE411

    Plasmid
    #62200
    Purpose
    CRISPR/Cas based plant genome editing and gene regulation; expresses 3×FLAG-NLS-zCas9-NLS, gRNA scaffold for insertion of target sequence (OsU3 promoter), Bar resistance
    Depositor
    Qi-Jun Chen
    Article
    Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.
    Inserts
    gRNA scaffold
    zCas9
    Use
    Plant binary vector
    Tags
    3x FLAG and NLS
    Expression
    Plant
    Mutation
    maize codon optimized
    Promoter
    OsU3 and Ubi
    Available Since
    April 29, 2015
    Availability
    Academic Institutions and Nonprofits only
    62200-plasmid-map-sequence-id-395472

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  30. pYPQ-CBE-Cas9n-Act3.0

    Plasmid
    #178955
    Purpose
    It consists of a Cas9 nickase (D10A) fused with a cytidine deaminase (A3A/Y130F) and MS2-SunTag-activators (ScFv-sfGFP-2xTAD), enabling simultaneous C to T conversion and gene activation.
    Depositor
    Yiping Qi
    Article
    Pan et al Nat Plants. 2022 May;8(5):513-525. doi:
    Insert
    hA3A-zCas9-UGI-T2A-MS2-SunTag-rbcS-E9t-ter-ZmUbi-scFv-sfGFP-2xTAD-GB1-NOS-ter
    Use
    CRISPR
    Expression
    Plant
    Available Since
    May 23, 2022
    Availability
    Academic Institutions and Nonprofits only
    178955-plasmid-map-sequence-id-458203

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  31. pLX-AS

    Plasmid
    #188870
    Purpose
    Compact T-DNA binary vector of the pLX series suitable for assembly of plant virus infectious clones and their Agrobacterium-mediated delivery to plants
    Depositor
    Fabio Pasin
    Article
    Pasin et al STAR Protoc. 2022 Dec 16;3(4):101716. do
    Insert
    none
    Use
    Synthetic Biology; Binary t-dna vector
    Expression
    Plant
    Promoter
    cauliflower mosaic virus (CaMV) 35S promoter
    Available Since
    Nov. 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    188870-plasmid-map-sequence-id-377941

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  32. pDIRECT_22D

    Plasmid
    #91136
    Purpose
    Direct Cloning, Type: T-DNA, Engineering Reagent: 35S:Csy4-P2A-AtCas9_dead + CmYLCV:gRNAs with Csy4 spacers, Plant Selection: 2x35S:npt II
    Depositor
    Daniel Voytas
    Article
    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.
    Insert
    Engineering Reagent: 35S:Csy4-P2A-AtCas9_dead + CmYLCV:gRNAs with Csy4 spacers
    Use
    CRISPR
    Expression
    Plant
    Mutation
    D10A, H840A
    Available Since
    July 11, 2017
    Availability
    Academic Institutions and Nonprofits only
    91136-plasmid-map-sequence-id-182475

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  33. pMpGWB337

    Plasmid
    #68061
    Purpose
    Gateway-based vector for Marchantia polymorpha to express a floxed gene, with an expression cassette for Cre-glucocorticoid receptor fusion protein under the control of heat-inducible promoter
    Depositor
    Takayuki Kohchi
    Article
    Nishihama et al Plant Cell Physiol. 2015 Jul 6. pii: pcv
    Type
    Empty backbone
    Use
    Cre/Lox
    Expression
    Plant
    Promoter
    MpEF1alpha promoter
    Available Since
    Sept. 18, 2015
    Availability
    Academic Institutions and Nonprofits only
    68061-plasmid-map-sequence-id-487277

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  35. pKb-TnpB1

    Plasmid
    #215333
    Purpose
    ISDra2 expression driven by PolII promoter ; 20 bp guide RNA can be cloned in BsaI site
    Depositor
    Kutubuddin Molla
    Article
    Karmakar et al Plant Biotechnol J. 2024 Jun 28. doi: 10
    Insert
    Rice codon optimized ISDra2 TnpB
    Tags
    Not applicable
    Expression
    Plant
    Promoter
    Rice ubiquitin promoter for TnpB and OsU3 promote…
    Available Since
    Sept. 24, 2024
    Availability
    Academic Institutions and Nonprofits only
    215333-plasmid-map-sequence-id-446611

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  37. pYPQ173

    Plasmid
    #99907
    Purpose
    Express CRISPR-Act2.0 system which contains pco-dCas9-VP64 fusion protein and MS2-VP64 fusion protein linked by in-frame T2A (encoding Thosea asigna 'self-cleaving' 2A peptide) sequence
    Depositor
    Yiping Qi
    Article
    Lowder et al Mol Plant. 2017 Nov 29. pii: S1674-2052(
    Insert
    pco-dCas9-VP64-T2A-MS2-VP64
    Use
    CRISPR
    Expression
    Plant
    Available Since
    May 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    99907-plasmid-map-sequence-id-210432

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Showing: 521 - 560 of 4625 results