4,069 results

1. pCMU-TPr

   Plasmid

   #61189

   Purpose

   Fluorescent reporter for tonoplast AtTIP1-1-mCherry expressed under AtUBQ10 promoter

   Depositor

   Maria Harrison

   Article

   Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

   Insert

   AtTIP1-1
   

   Use

   Tags

   mCherry

   Expression

   Plant

   Mutation

   Promoter

   AtUBQ10

   Available since

   Feb. 17, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSV40-mCherry-P2A-Hygro

   Plasmid

   #106478

   Purpose

   Expression of mCherry and Hygromycin resistance gene from SV40 promoter

   Depositor

   Alex Hewitt

   Inserts

   mCherry
   Hygromycin B resistance
   

   Use

   Tags

   Expression

   Mammalian and Plant

   Mutation

   Promoter

   SV40 promoter

   Available since

   April 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDGB3 alpha2 P35S:dCas9:TV:Tnos (GB2047)

   Plasmid

   #160626

   Purpose

   TU for the constitutive expression of dCas9 fused to TV (TALx6 - VP128) activation domains

   Depositor

   Diego Orzaez

   Article

   Selma et al Plant Biotechnol J. 2019 Sep;17(9):1703-

   Insert

   P35s-dCas9:TV-Tnos
   

   Use

   Tags

   Expression

   Plant

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   P35S

   Available since

   March 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pGWB405

   Plasmid

   #74799

   Purpose

   Gateway cloning compatible binary vector for C-terminal fusion with sGFP (CaMV35S promoter).

   Depositor

   Tsuyoshi Nakagawa

   Article

   Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

   Type

   Empty backbone

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   CaMV35S

   Available since

   July 18, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pYPQ133B2.0

   Plasmid

   #99892

   Purpose

   Golden Gate entry vector to express the 3rd gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under AtU3 promoter

   Depositor

   Yiping Qi

   Article

   Lowder et al Mol Plant. 2017 Nov 29. pii: S1674-2052(

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   AtU3

   Available since

   March 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. MT363

   Plasmid

   #65858

   Purpose

   The gRNA construct is designed for constitutive expression of salicylate hydroxylase for degradation of salicylic acid to catechol.

   Depositor

   Detlef Weigel

   Article

   Todesco et al Nature. 2010 Jun 3;465(7298):632-6. doi:

   Insert

   nahG
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Sept. 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCHW111117

   Plasmid

   #222460

   Purpose

   Level 1 acceptor, Position 3 containing CBS4-CMV1::LoxP-mCherry-stop-LacZ::nosT.

   Depositor

   Chih-Hang Wu

   Article

   Chiang et al bioRxiv 2024.03.23.586378

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   no

   Promoter

   CBS4-CMV1 (Cucumber mosaic virus)

   Available since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMpGWB120

   Plasmid

   #68574

   Purpose

   Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

   Depositor

   Takayuki Kohchi

   Article

   Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

   Type

   Empty backbone

   Use

   Tags

   Modified EAR motif plant-specific repression doma…

   Expression

   Plant

   Mutation

   Promoter

   Available since

   June 24, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pMKV060

   Plasmid

   #133315

   Purpose

   BeYDV viral replicon on T-DNA backbone expressing Firefly Luc+

   Depositor

   Daniel Voytas

   Article

   Maher et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

   Insert

   Luc+
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   AtUbi10

   Available since

   Dec. 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. ePE2

    Plasmid

    #202029

    Purpose

    Prime Editing plasmid for tag insertion

    Depositor

    Pengcheng Wei

    Article

    Li et al Plant Commun. 2023 Mar 7:100572. doi: 10

    Insert

    Cas9，
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Aug. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pZA26

    Plasmid

    #158504

    Purpose

    In planta gene expression of Golden gate compatible A. thaliana ZAR1 D498V-4xMyc. Km plant selection marker.

    Depositor

    Sophien Kamoun

    Article

    Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

    Insert

    ZAR1-4xMyc (ZAR1 Mustard Weed)
    

    Use

    Tags

    4xMyc

    Expression

    Plant

    Mutation

    D489V

    Promoter

    Available since

    Aug. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGWB513

    Plasmid

    #74855

    Purpose

    Gateway cloning compatible binary vector for C-terminal fusion with 3xHA (no promoter).

    Depositor

    Tsuyoshi Nakagawa

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    July 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pGPTVII-Bar-U-MatryoshCaMP6s

    Plasmid

    #100024

    Purpose

    Plant expression of fluorescent reporter for calcium signaling, based off of GCaMP6s. Contains a stable reference Large Stokes Shift (LSS) mOrange nested within the reporting cpEGFP.

    Depositor

    Wolf Frommer

    Article

    Ast et al Nat Commun. 2017 Sep 5;8(1):431. doi: 10

    Insert

    MatryoshCaMP6s
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    AtUBQ10

    Available since

    Oct. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pMM113

    Plasmid

    #127210

    Purpose

    BeYDV replicon with constitutive Luc expression, WUS2 and AtSTM, sgRNA targeting PDS

    Depositor

    Daniel Voytas

    Article

    Maher et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

    Insert

    WUS2, AtSTM, sgRNA, Luc
    

    Use

    Tags

    Expression

    Plant

    Mutation

    codon optimized CDSs

    Promoter

    Available since

    Dec. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pBiFCt-2in1-CC

    Plasmid

    #105114

    Purpose

    ratiometric BiFC analysis in plants (transient transformation)

    Depositor

    Christopher Grefen

    Article

    Grefen et al Biotechniques. 2012 Oct 2;53(5):311-14.

    Type

    Empty backbone

    Use

    Tags

    cYFP-Myc/nYFP-HA

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Feb. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCHW110033

    Plasmid

    #222441

    Purpose

    Level 0, CDS module (AATG-GCTT) containing Cre recombinase.

    Depositor

    Chih-Hang Wu

    Article

    Chiang et al bioRxiv 2024.03.23.586378

    Insert

    Cre recombinase
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    BsaI/BbsI sites removed by point-mutation

    Promoter

    Available since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pYPQ141B2.0

    Plasmid

    #99897

    Purpose

    Express single gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under AtU3 promoter

    Depositor

    Yiping Qi

    Article

    Lowder et al Mol Plant. 2017 Nov 29. pii: S1674-2052(

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    AtU3

    Available since

    March 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pEGB 35S:YFP:Tnos (GB0209)

    Plasmid

    #68219

    Purpose

    Transcriptional unit for Yellow Flurescent Protein plant expression driven by the 35S promoter

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    YFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    35S

    Available since

    Oct. 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pGTQL1211YN

    Plasmid

    #61704

    Purpose

    Gateway compatible BiFC vector (pEarleyGate201-YN)

    Depositor

    Yuhai Cui

    Article

    Lu et al Plant J. 2010 Jan;61(2):259-70. doi: 10.

    Type

    Empty backbone

    Use

    Bifc assay

    Tags

    YFP-N and HA

    Expression

    Plant

    Mutation

    Promoter

    35S

    Available since

    May 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pGTQL1221YC

    Plasmid

    #61705

    Purpose

    Gateway compatible BiFC vector (pEarleyGate202-YC)

    Depositor

    Yuhai Cui

    Article

    Lu et al Plant J. 2010 Jan;61(2):259-70. doi: 10.

    Type

    Empty backbone

    Use

    Bifc assay

    Tags

    YFP-C and FLAG

    Expression

    Plant

    Mutation

    Promoter

    35S

    Available since

    May 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge