4,069 results

1. pAGM55273

   Plasmid

   #153211

   Purpose

   Level 2 cloning vector for a single guide RNA, already contains Cas9, Kanamycin selection cassette for plant transformation and FAST marker

   Depositor

   Sylvestre Marillonnet

   Article

   Grutzner et al Plant Commun. 2020 Nov 23;2(2):100135. d

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Aug. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. UBQ10:sXVE:(MCS)-S11-DI-GFP1–9

   Plasmid

   #108260

   Purpose

   Binary vectors used for the inducible expression of dual intein-coupled tripartite split-sfGFP components in plants

   Depositor

   Tzu-Yin Liu

   Article

   Liu et al Plant J. 2018 Feb 16. doi: 10.1111/tpj.1

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   Promoter

   UBQ10:sXVE:

   Available since

   April 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pYPQ265E2

   Plasmid

   #164719

   Purpose

   Gateway entry clone (attL1 & attR5) A3A/Y130F-CBE_V01 for C-T base editing

   Depositor

   Yiping Qi

   Article

   Ren et al Plant Biotechnol J. 2021 May 27. doi: 10

   Insert

   A3A(Y130F)-zCas9(D10A)-UGI
   

   Use

   CRISPR; Gateway compatible a3a/y130f-cbe_v01 entr…

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   May 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHEE401

   Plasmid

   #71286

   Purpose

   Egg cell-specific promoter-controlled expression of 3×FLAG-NLS-zCas9-NLS. Contains gRNA scaffold for insertion of target sequence (U6-26 promoter), Hyg resistance

   Depositor

   Qi-Jun Chen

   Article

   Wang et al Genome Biol. 2015 Jul 21;16:144. doi: 10

   Inserts

   sgRNA scaffold
   zCas9
   

   Use

   CRISPR; Plant binary vector

   Tags

   3x FLAG and NLS

   Expression

   Plant

   Mutation

   Zea mays codon-optimized Cas9

   Promoter

   EC1.2 promoter and U6-26p Arabidopsis U6 gene pro…

   Available since

   Jan. 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTRANS_220

   Plasmid

   #91113

   Purpose

   Transformation, Type: T-DNA, Plant Selection: 2x35S:npt II, Viral Replication: none

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Type

   Empty backbone

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   35S

   Available since

   July 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. p35S_GFP-GUS

   Plasmid

   #167122

   Purpose

   Plant binary expression vector containing mGFP5-GUS fusion reporter under CaMV 35S promoter.

   Depositor

   Olivier Voinnet, Arturo Marí-Ordóñez

   Article

   Oberlin et al Genome Res. 2017 Sep;27(9):1549-1562. do

   Insert

   GFP-GUS
   

   Use

   Tags

   mGFP5, GUS

   Expression

   Plant

   Mutation

   Promoter

   CaMV 35S

   Available since

   May 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLCS111

   Plasmid

   #209332

   Purpose

   Gateway cloning compatible vector for transient expression in protoplasts. Arabidopsis UBQ10 promoter expression of N-terminal mScarlet-I-mNeonGreen tandem fluorescent timer fusion protein.

   Depositor

   Lucia Strader

   Article

   Allen et al Res Sq. 2023 Nov 7:rs.3.rs-3558280. doi:

   Type

   Empty backbone

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Jan. 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMC-6-StayGold-9

   Plasmid

   #197734

   Purpose

   Phytobrick (MoClo) Level 0 Part

   Depositor

   Daniel Voytas

   Article

   Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

   Insert

   StayGold GFP
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   July 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pKI_ΩBBMVP16&SRDXWUSm1

   Plasmid

   #220349

   Purpose

   Expresses BBM-VP16 and SRDX-WUSm1 in plant cells

   Depositor

   Tomoko Igawa

   Article

   Sato et al Front Plant Sci. 2024 Apr 3;15:1308417.

   Inserts

   BABY BOOM (BBM Mustard Weed)
   WUSCHEL (WUS Mustard Weed)
   

   Use

   Tags

   Superman Repression Domain X (SRDX) and VP16 tran…

   Expression

   Plant

   Mutation

   two amino acid substitution in WUS-box: L255A L25…

   Promoter

   CaMV 35S and RPS5A

   Available since

   July 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pYLCRISPR/Cas9P35S-B

    Plasmid

    #66190

    Purpose

    expression of Cas9 in plants, cauliflower mosaic virus 35S promoter (P35S), basta selection

    Depositor

    Yao-Guang Liu

    Article

    Ma et al Mol Plant. 2015 Apr 24. pii: S1674-2052(

    Insert

    Cas9
    

    Use

    Tags

    Expression

    Plant

    Mutation

    plant-codon optimized with higher GC contents (62…

    Promoter

    cauliflower mosaic virus 35S promoter (P35S)

    Available since

    Oct. 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pbZIP9:GFP-GUS

    Plasmid

    #172483

    Purpose

    GFP-GUS driven by the bZIP9 promoter

    Depositor

    Wolf Frommer

    Article

    Kim et al Plant Cell. 2021 May 5;33(3):511-530. do

    Insert

    bZIP9 promoter (BZIP9 Mustard Weed)
    

    Use

    Tags

    GFP and GUS

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Aug. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. P16ΔS:S10-(MCS)

    Plasmid

    #108182

    Purpose

    Binary vectors used for the constitutive expression of tripartite split-sfGFP components in plants

    Depositor

    Tzu-Yin Liu

    Article

    Liu et al Plant J. 2018 Feb 16. doi: 10.1111/tpj.1

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    P16ΔS

    Available since

    April 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. MT-AvrXa10-FT

    Plasmid

    #234373

    Purpose

    T-DNA vector for expression of the two protein components of the MoonTag system (dCas9-24XGP41 and NbGP41P-GFP-AvrXa10-GB1) with optimized activation domain (AvrXa10), targeting Arabidopsis FT locus

    Depositor

    Michael Smanski

    Article

    Zinselmeier et al Plant Biotechnol J. 2024 Nov;22(11):3202

    Inserts

    NbGP41-GFP-AvrXa10-GB1
    dCas9-24XGP41
    gRNAs targeting FT locus
    RFP
    

    Use

    Synthetic Biology

    Tags

    GB1, GFP, and GP41

    Expression

    Plant

    Mutation

    Promoter

    Arabidopsis U6, Arabidopsis Ubi10, and Arabidopsi…

    Available since

    April 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. NST1_pOpOn2.1

    Plasmid

    #228065

    Purpose

    Expresses NST1 in different cell types in Arabidopsis thaliana upon induction with dexamethasone.

    Depositor

    Charles Anderson

    Article

    Tamadaddi et al Ann Bot. 2024 Aug 30:mcae153. doi: 10.10

    Insert

    NAC SECONDARY WALL THICKENING PROMOTING FACTOR1, NST1 (NST1 Mustard Weed)
    

    Use

    Dexamethasone inducible vector

    Tags

    Expression

    Plant

    Mutation

    Promoter

    CaMV35S for LhGR and pOp6-minimal CaMV35S for NST1

    Available since

    Jan. 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pKAN43Ag8-GFP

    Plasmid

    #178182

    Purpose

    Plant binary vector expressing GFP by a enhanced 35S promoter

    Depositor

    Keiichi Shimizu

    Insert

    EGFP (eGFP )
    

    Use

    Tags

    Expression

    Plant

    Mutation

    The CAT is inserted after the start codon.

    Promoter

    The minimal 35S promoter from Cauliflower Mosaic …

    Available since

    Sept. 26, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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16. pGWB510

    Plasmid

    #74852

    Purpose

    Gateway cloning compatible binary vector for C-terminal fusion with FLAG (no promoter).

    Depositor

    Tsuyoshi Nakagawa

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Sept. 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. puc19-pCas13a

    Plasmid

    #118963

    Purpose

    Intermediate/cloning vector that can be used for sub-cloning into final destination vectors based on one's requirements.

    Depositor

    Magdy Mahfouz

    Article

    Aman et al Genome Biol. 2018 Jan 4;19(1):1. doi: 10

    Insert

    Cas13a
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Feb. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGWB402-omega

    Plasmid

    #74797

    Purpose

    Gateway cloning compatible binary vector for expression of gene by 2xCaMV35S enhancer and omega sequence.

    Depositor

    Tsuyoshi Nakagawa

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    2xCaMV35S enhancer and omega

    Available since

    July 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pRGEB32-BAR

    Plasmid

    #126072

    Purpose

    For CRISPR/Cas9 delivery in maize. Expresses Cas9 with rice ubiquitin promoter, BAR with 35S promoter, and sgRNA/PTG with rice snoRNA U3 promoter (pol III). Can produce 1 or more gRNAs.

    Depositor

    Charles Hunter

    Article

    Hunter et al Plants (Basel). 2021 Apr 8;10(4). pii: p

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    35S for BAR; Rice UBI for Cas9; Rice snoRNA U3 fo…

    Available since

    June 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pPSup

    Plasmid

    #149416

    Purpose

    Plasmid for STARR-seq in tobacco leaves. Enhancer candidates can be inserted just upstream of the minimal promoter.

    Depositor

    Stan Fields

    Article

    Jores et al Plant Cell. 2020 May 14. pii: tpc.20.001

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    July 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge