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Plasmid#91112PurposeTransformation, Type: T-DNA, Plant Selection: 2x35S:hpt II, Viral Replication: WDVDepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoter35SAvailable sinceSept. 29, 2017AvailabilityAcademic Institutions and Nonprofits only
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pCaRNA1&2
Plasmid#86087PurposeSimultaneous expression of cDNAs of complete genomic RNA1 and RNA 2 of PNRSV (Pch12 isolate) in transfected cellsDepositorInsertRNA1, RNA2
UseTagsExpressionPlantMutationPromoter35SAvailable sinceFeb. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pYPQ265E6
Plasmid#213469PurposeGateway entry clone (attL1 & attR5) TadA-CDa-32aa linker-zSpCas9-D10A-2xUGI for C-T base editingDepositorInsertTadA-CDa-32aa linker-zSpCas9-D10A-2xUGI
UseCRISPR; Gateway compatible tada-cda-32aa linker-z…TagsNoExpressionPlantMutationPromoterAvailable sinceMay 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
dpYPQ230-D156R-ABE4
Plasmid#195370PurposedLbCas12a-D156R based adenine base editor, ecTadA8e fused to the N terminal of dLbCas12a-D156R with the linker of (GGGGS)x6DepositorInsertecTadA8e-(GGGGS)x6-dLbCas12a-D156R
UseCRISPR; Gateway compatible ectada8e-(ggggs)x6-dlb…Tags5' and 3' NLSExpressionPlantMutationPromoterZmUBI1Available sinceMay 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
dpYPQ230-D156R-CBE1
Plasmid#195367PurposedLbCas12a-D156R based cytosine base editor, hA3A/Y130F fused to the N terminal of dLbCas12a-D156R with the linker of XTEN with 3xFLAGDepositorInserthA3A/Y130F-XTEN with 3xFLAG-dLbCas12a-D156R-UGI
UseCRISPR; Gateway compatible ha3a/y130f-xten plus 3…Tags3xflagExpressionPlantMutationPromoterZmUBI1Available sinceMay 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
Raichu-OsRac1 WT
Plasmid#133262PurposeBiosensor for monitoring activation for rice small GTPase OsRac1DepositorInsertOsRac1 (LOC4325879 Oryza sativa)
UseTagsCFP-lipid and Venus-CRIBExpressionPlantMutationPromoterUbiAvailable sinceDec. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB229
Plasmid#68620PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneUseTagstdTomatoExpressionPlantMutationPromoterAvailable sinceNov. 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCaRNA3
Plasmid#86088PurposeExpression of cDNA of full-length genomic RNA3 of PNRSV (Pch12 isolate) in transfected cellsDepositorInsertRNA3
UseTagsExpressionPlantMutationPromoter35SAvailable sinceJan. 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHDE-35S-Cas9-mCherry
Plasmid#78931PurposeFor CRISPR/Cas9 mediated gene editing in Arabidopsis; provides a visual screen for Cas9-free plantsDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceJuly 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGrDL_SPb
Plasmid#83205Purposereceives miRNA target sequences for testing using the dual luciferase assay systemDepositorInsertTomato ACTIN promoter
UseTagsExpressionPlantMutationPromoterTomato ACTINAvailable sinceNov. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTRANS_100
Plasmid#91198PurposeTransformation, Type: non-T-DNA, Plant Selection: none, Viral Replication: noneDepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceJuly 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMC-6-RedFLuc-9
Plasmid#197738PurposePhytobrick (MoClo) Level 0 PartDepositorInsertRedF Luciferase (tomato optimized)
UseTagsExpressionPlantMutationPromoterAvailable sinceMay 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDEST17-UnaG
Plasmid#203485PurposeFor recombinant protein production in Escherichia coli. His-tagged UnaG under the control of the T7 promoter.DepositorInsertUnaG
UseTagsExpressionBacterial and PlantMutationPromoterAvailable sinceAug. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFH14
Plasmid#125799PurposeLevel0 SaCas9, Arabidopsis codon optimizedDepositorInsertSaCas9, Arabidopsis codon optimized
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTRANS_150
Plasmid#91205PurposeTransformation, Type: non-T-DNA, Plant Selection: PvUbi2:hpt II, Viral Replication: noneDepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceJuly 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pYPQ135B2.0
Plasmid#167159PurposeGolden Gate entry vector to express the 5th gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under AtU3 promoterDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterAtU3Available sinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHdzCas9-KRAB
Plasmid#92341PurposeExpression dzCas9-KRAB in microalgae. CRISPR/Cas based plant genome editing and gene regulation, Hyg resistanceDepositorTypeEmpty backboneUseCRISPR; Plant expressionTagsKRABExpressionPlantMutationPromoterCaMV35SAvailable sinceJune 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
GB4070
Plasmid#187811PurposeModule for the constitutive expression of Cup2:Gal4AD and gRNA-1 DFR, and the copper-inducible expression of dCasEV2.1 (dCas9:EDLL and MS2:VPR).DepositorInsertcopper-inducible dCasEV2.1
UseTagsExpressionPlantMutationPromoterAvailable sinceJune 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pNK869
Plasmid#219737PurposeMoClo-compatible Level 0 - promoter + 5U vector carrying promoter AtPD7DepositorInsertpromoter of serine carboxypeptidase-like gene AtSCPL30, AtPD7
UseSynthetic BiologyTagsExpressionPlantMutationPromoterAtPD7Available sinceJuly 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUBQ10-mNeonGreen-GW
Plasmid#209352PurposeGateway cloning compatible binary vector for agrobacterium mediated transformation. Arabidopsis UBQ10 promoter expression of N-terminal mNeonGreen fusion protein.DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceFeb. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pH-dCas9-PBE
Plasmid#98162PurposeExpresses CRISPR base editor in riceDepositorInsertNLS-APOBEC1-XTEN-dCas9-UGI-NLS
UseRiceTagsExpressionPlantMutationPromoterUbiAvailable sinceJuly 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pH-Ubi-miniSdd7-nCas9-PBE
Plasmid#204856PurposeFor cytosine base editing using miniSdd7 in monocotyledon plants (Agrobacterium-mediated transformation)DepositorInsertminiSdd7-nSpCas9(D10A)-UGI
UseCRISPRTagsExpressionPlantMutationD10A in SpCas9PromoterZmUbi-1, OsU3, 35SAvailable sinceAug. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pARSCL504
Plasmid#198636PurposeExpresses a transgene encoding RUBYDepositorInsert35S:Ω:Ruby
UseTagsExpressionPlantMutationPromoterAvailable sinceJune 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHAtC
Plasmid#78098PurposePlant expression of Cas9 and gRNA empty backbone; Hygro ResistanceDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterU6Available sinceJune 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
AL13Rb
Plasmid#161006PurposePP7-tagged reporter without a promoterDepositorTypeEmpty backboneUseTagsPP7 and H2B-mScarletExpressionPlantMutationPromoterAvailable sinceDec. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPSup_iGFP
Plasmid#149420PurposePlasmid for STARR-seq in tobacco leaves. Enhancer candidates can be inserted just upstream of the minimal promoter. The GFP reporter gene harbors the IV2 intron.DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceJuly 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pZA72
Plasmid#174783PurposeIn planta gene expressionDepositorInsertGG_XopJ4, Kmr plant selection marker
UseTagsExpressionPlantMutationWT + STOP codonPromoterAvailable sinceOct. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
P427-FBP_13
Plasmid#139704PurposeT-DNA encoding Fungal Bioluminescent Pathway Components, complete pathwayDepositorInsertpSlH4:NnCPH:tSlH4, pSlRBCS2:AsNpga:tAtug7, pUBQ1:NhH3H:tNos, p35S:NnHisps:t35S, pAtELF4:NnLuz:trbcse9
UseLuciferaseTagsExpressionPlantMutationPromotervariousAvailable sinceMay 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTC364
Plasmid#91213Purposeprotoplast vector expressing 2 gRNAs targeting tomato ARF8A (CmYLCV promoter, Csy4 processing)DepositorInsert2 gRNAs targeting tomato ARF8A
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceJuly 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pICH47802-p35S::tdTomato-ER::tNOS
Plasmid#160220PurposetdTomato fluorescent protein-ER localization, 35 promoter, tNOS terminator, transcriptional unit, inserted at the MoClo toolkit level 1 acceptor position 1 reverseDepositorInsertp2x35S::TdTomato-ER::tNOS
UseSynthetic Biology; Moclo toolkit level 1 acceptor…TagsExpressionPlantMutationPromoterAvailable sinceAug. 15, 2022AvailabilityAcademic Institutions and Nonprofits only