4,625 results

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3TERM_35S

Plasmid

#136114

Purpose

3TERM after CDS/ CDS12-CTAG

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

3TERM_35S (CaMV)

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available Since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pRATIO3212

Plasmid

#141311

Purpose

Gateway cloning compatible dual fluorescent ratiometric binary vector

Depositor

David Nelson

Article

Khosla et al Plant Direct. 2020 Jun 21;4(6):e00231. d

Type

Empty backbone

Tags

mScarlet-I and mVenus

Expression

Plant

Promoter

CaMV 35S promoter

Available Since

Sept. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pYPQ141-ZmUbi-RZ-Fn

Plasmid

#108864

Purpose

FnCpf1 Gateway crRNA entry plasmid using Zea mays Ubi promoter and double ribozyme processing

Depositor

Yiping Qi

Article

Zhong et al Mol Plant. 2018 Jul 2;11(7):999-1002. do

Type

Empty backbone

Use

CRISPR; Gateway compatible crrna entry clone

Expression

Plant

Available Since

Aug. 27, 2018

Availability

Academic Institutions and Nonprofits only

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pAK005

Plasmid

#128218

Purpose

improved sgRNA backbone for tRNA-gRNA array, Position 5

Depositor

Vladimir Nekrasov

Article

Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

Insert

improved sgRNA backbone for tRNA-gRNA array, Position 5

Use

CRISPR and Synthetic Biology

Expression

Plant

Available Since

Oct. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pAK004

Plasmid

#128217

Purpose

improved sgRNA backbone for tRNA-gRNA array, Position 4

Depositor

Vladimir Nekrasov

Article

Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

Insert

improved sgRNA backbone for tRNA-gRNA array, Position 4

Use

CRISPR and Synthetic Biology

Expression

Plant

Available Since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pAK006

Plasmid

#128219

Purpose

improved sgRNA backbone for tRNA-gRNA array, Position 6

Depositor

Vladimir Nekrasov

Article

Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

Insert

improved sgRNA backbone for tRNA-gRNA array, Position 6

Use

CRISPR and Synthetic Biology

Expression

Plant

Available Since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pGem-nHyPer:cAPX

Plasmid

#84736

Purpose

Simultaneous expresion of stromal HyPer2 and cytosolic Ascorbate peroxidase (APX) in plant cells

Depositor

Philip Mullineaux

Article

Exposito-Rodriguez et al Nat Commun. 2017 Jun 29;8(1):49. doi: 10

Inserts

APX1 (APX1 Mustard Weed)
HyPer2 targeted to nucleus

Tags

NLS-SV40

Expression

Plant

Promoter

pFMV/p35S

Available Since

June 30, 2017

Availability

Academic Institutions and Nonprofits only

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pYPQ265_OoA3GX2

Plasmid

#225147

Purpose

Gateway entry clone (attL1 & attR5) OoA3GX2-CBE_V01 for C-T base editing

Depositor

Yiping Qi

Article

Contiliani et al Plant Biotechnol J. 2025 Feb 14. doi: 10

Insert

OoA3GX2-zCas9(D10A)-UGI

Expression

Plant

Available Since

Feb. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pGrDL_SP

Plasmid

#83204

Purpose

receives miRNA target sequences for testing using the dual luciferase assay system

Depositor

Peer Schenk

Article

Moyle et al Front Plant Sci. 2017 Sep 20;8:1631. doi

Insert

SalI/PstI cloning sites

Expression

Plant

Available Since

Nov. 3, 2016

Availability

Academic Institutions and Nonprofits only

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pFH66

Plasmid

#131765

Purpose

Level1 ZmUBIp::SpCas9 (wheat codon optimized, version 2):NOSt

Depositor

Vladimir Nekrasov

Article

Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

Insert

ZmUBIp::SpCas9 (wheat codon optimized, version 2):NOSt

Use

CRISPR and Synthetic Biology

Expression

Plant

Available Since

Oct. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pMpGWB130

Plasmid

#68584

Purpose

Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

Depositor

Takayuki Kohchi

Article

Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

Type

Empty backbone

Tags

tdTomato

Expression

Plant

Promoter

Cauliflower mosaic virus 35S promoter

Available Since

June 24, 2016

Availability

Academic Institutions and Nonprofits only

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pCHW00019

Plasmid

#222448

Purpose

Level 0, C terminal tag module (TTCG-GCTT) containing GAL4AD-NLS.

Depositor

Chih-Hang Wu

Article

Chiang et al New Phytol. 2024 Oct;244(1):318-331. doi

Insert

GAL4AD-NLS

Use

Synthetic Biology

Expression

Plant

Mutation

no

Available Since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pCHW100003

Plasmid

#222442

Purpose

Level 0, N-terminal tag module (CCAT-AATG) containing mCherry2-HyP5SM.

Depositor

Chih-Hang Wu

Article

Chiang et al New Phytol. 2024 Oct;244(1):318-331. doi

Insert

mCherry2-HyP5SM

Use

Synthetic Biology

Expression

Plant

Mutation

no

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pCHW111114

Plasmid

#222459

Purpose

Level 1 acceptor, Position 3 containing CBS4-CMV1::LacZ::nosT.

Depositor

Chih-Hang Wu

Article

Chiang et al New Phytol. 2024 Oct;244(1):318-331. doi

Type

Empty backbone

Use

Synthetic Biology

Expression

Plant

Mutation

no

Promoter

CBS4-CMV1 (Cucumber mosaic virus)

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pCHW111118

Plasmid

#222461

Purpose

Level 1 acceptor, Position 3 containing CBS4-CMV1::LoxP-mCherry-stop-LacZ::nosT.

Depositor

Chih-Hang Wu

Article

Chiang et al New Phytol. 2024 Oct;244(1):318-331. doi

Type

Empty backbone

Use

Synthetic Biology

Expression

Plant

Mutation

no

Promoter

CBS4-CMV1 (Cucumber Mosaic Virus)

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pCHW111113

Plasmid

#222458

Purpose

Level 1 acceptor, Position 3 containing CBS4-CMV1::LacZ::nosT.

Depositor

Chih-Hang Wu

Article

Chiang et al New Phytol. 2024 Oct;244(1):318-331. doi

Type

Empty backbone

Use

Synthetic Biology

Expression

Plant

Mutation

no

Promoter

CBS4-CMV1 (Cucumber mosaic virus)

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pCHW100002

Plasmid

#222440

Purpose

Level 0, CDS module (AATG-GCTT) containing rice OsL5.

Depositor

Chih-Hang Wu

Article

Chiang et al New Phytol. 2024 Oct;244(1):318-331. doi

Insert

OsL5 (Oryza sativa)

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/BbsI sites removed by point-mutation

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pCHW91003

Plasmid

#222449

Purpose

Level 1, Position 3 containing 35S::RUBY::OCS.

Depositor

Chih-Hang Wu

Article

Chiang et al New Phytol. 2024 Oct;244(1):318-331. doi

Insert

35S::RUBY::OCS

Use

Synthetic Biology

Expression

Plant

Mutation

no

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pGWB450

Plasmid

#74832

Purpose

Gateway cloning compatible binary vector for C-terminal fusion with G3GFP (no promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Expression

Plant

Available Since

Oct. 18, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pMpGWB335

Plasmid

#68663

Purpose

Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

Depositor

Takayuki Kohchi

Article

Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

Type

Empty backbone

Tags

TagRFP

Expression

Plant

Promoter

Cauliflower mosaic virus 35S promoter

Available Since

July 13, 2016

Availability

Academic Institutions and Nonprofits only

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pTRANS_233

Plasmid

#91122

Purpose

Transformation, Type: T-DNA, Plant Selection: 2x35S:bar, Viral Replication: WDV

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Type

Empty backbone

Expression

Plant

Promoter

35S

Available Since

Sept. 29, 2017

Availability

Academic Institutions and Nonprofits only

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pMpGWB403

Plasmid

#68668

Purpose

Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

Depositor

Takayuki Kohchi

Article

Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

Type

Empty backbone

Expression

Plant

Promoter

MpEF1alpha promoter

Available Since

June 24, 2016

Availability

Academic Institutions and Nonprofits only

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pNC-Green-LUC

Plasmid

#193438

Purpose

Nimble cloning-compatible vector for the analysis of promoter activity in plants

Depositor

Pu Yan

Article

Yan et al Plant Commun. 2023 Mar 13;4(2):100471. d

Type

Empty backbone

Expression

Plant

Available Since

Dec. 13, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
barII-UT-ABAleon2.1

Plasmid

#106981

Purpose

ABAleon2.1 in plant expression vector (Basta resistance)

Depositor

Julian Schroeder

Article

Waadt et al Elife. 2014 Apr 15;3:e01739. doi: 10.755

Insert

ABAleon2.1

Expression

Plant

Promoter

UBQ10

Available Since

Feb. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pARR5::LUC

Plasmid

#141170

Purpose

Luminescent reporter for cytokinin signaling

Depositor

Vardis Ntoukakis, Patrick Schäfer

Article

Lehmann et al PLoS One. 2020 Jun 4;15(6):e0234154. doi

Insert

AtARR5 promoter

Use

Luciferase

Expression

Plant

Promoter

AtARR5, AT3G48100

Available Since

Aug. 10, 2020

Availability

Academic Institutions and Nonprofits only

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A3A-Gam

Plasmid

#119769

Purpose

Conversion of C to T monocotyledons protoplasts

Depositor

Caixia Gao

Article

Zong et al Nat Biotechnol. 2018 Oct 1. pii: nbt.426

Insert

Gam-APOBEC3A-nCas9-NLS-2XUGI-NLS

Tags

NLS (flanking UGI)

Expression

Plant

Mutation

nCas9 has a mutation D10A in wild type SpCas9

Promoter

maize Ubiquitin-1

Available Since

Sept. 25, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pGem-sHyper:sAPX

Plasmid

#84738

Purpose

Simultaneous expresion of stromal sHyPer and stromal Ascorbate peroxidase (APX) in plant cells

Depositor

Philip Mullineaux

Article

Exposito-Rodriguez et al Nat Commun. 2017 Jun 29;8(1):49. doi: 10

Inserts

sAPX (SAPX Mustard Weed)
sHyper (HyPer2 targeted to nucleus)

Tags

nuclear localisation signals from the coding sequ…

Expression

Plant

Promoter

pFMV/p35S

Available Since

July 6, 2017

Availability

Academic Institutions and Nonprofits only

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pMT1-24X-NOG(CmYLCV)

Plasmid

#202014

Purpose

T-DNA vector for expression of the two protein components of the MoonTag system (dCas9-24XGP41 and NbGP41P-sfGFP-VP64-GB1) under the control of the CmYLCV promoter.

Depositor

Michael Smanski

Article

Casas-Mollano et al Nucleic Acids Res. 2023 Jul 21;51(13):70

Inserts

dCas9-24XGP41
NbGP41P-sfGFP-VP64-GB1

Tags

GB1 and sfGFP

Expression

Plant

Promoter

CmYLCV

Available Since

Aug. 21, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
AL12R-HSP70

Plasmid

#161010

Purpose

PP7-tagged reporter driven by the Arabidopsis HSP70 promoter

Depositor

Krishna Niyogi

Article

Alamos et al Nat Plants. 2021 Aug;7(8):1037-1049. doi

Insert

PP7 and H2B-mScarlet

Expression

Plant

Available Since

Dec. 8, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pH7m34GW-35S:SNACS S785A

Plasmid

#203577

Purpose

Plant SnRK2S30A kinase activity FRET sensor in plant expression vector

Depositor

Julian Schroeder

Article

Zhang et al eLife. 2020 May 28;9:e56351. doi: 10.755

Insert

SNACS S785A

Tags

StrepII

Expression

Plant

Mutation

S785A

Available Since

Aug. 10, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pEE531

Plasmid

#149290

Purpose

T-DNA encoding TRV2 with spacer multiplexed truncated FT augmented gRNAs targeting NbAGsgRNA1, NbPDS3, NbAGsgRNA2

Depositor

Daniel Voytas

Article

Ellison et al Nat Plants. 2020 Jun 1. pii: 10.1038/s41

Insert

p35S:TRV2_Spacer_NbAGsgRNA1_NbPDS3sgRNA_NbAGsgRNA2_TrunFT:tNos

Expression

Plant

Promoter

35S

Available Since

July 20, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pFH102

Plasmid

#128207

Purpose

sgRNA backbone for SaCas9, combine with AtU6-26 promoter module (pFH35)

Depositor

Vladimir Nekrasov

Article

Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

Insert

sgRNA backbone for SaCas9, combine with AtU6-26 promoter module (pFH35)

Use

CRISPR and Synthetic Biology

Expression

Plant

Available Since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pAGM65905

Plasmid

#153217

Purpose

Level 2 cloning vector for one or several guide RNAs, already contains Cas9 and a kanamycin selection cassette for plant transformation

Depositor

Sylvestre Marillonnet

Article

Grutzner et al Plant Commun. 2020 Nov 23;2(2):100135. d

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available Since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pICSL11055

Plasmid

#68252

Purpose

Level 1 Golden Gate Cassette: Plant kanamycin resistance cassette

Depositor

Nicola Patron

Article

Lawrenson et al Genome Biol. 2015 Nov 30;16(1):258. doi:

Insert

Promoter:2x35s+5'UTR:Omega+CDS:nptII+3'UTR/terminator:Nos

Use

Synthetic Biology

Expression

Plant

Available Since

Sept. 1, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pCsC_spacer

Plasmid

#136077

Purpose

Use when making odd level constructs (L3,...) if pCsC position (position 3) has no construct assigned

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

Even spacer

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available Since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pCsE_spacer

Plasmid

#136078

Purpose

Use when making odd level constructs (L3,...) if pCsE position (position 4) has no construct assigned

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

Even spacer

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available Since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pCsB

Plasmid

#136068

Purpose

Acceptor plasmid for even level position 2, lacZ blue-white screening

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

lacZ

Use

Synthetic Biology

Expression

Plant

Available Since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pCsC

Plasmid

#136069

Purpose

Acceptor plasmid for even level position 3, lacZ blue-white screening

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

lacZ

Use

Synthetic Biology

Expression

Plant

Available Since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pCsE

Plasmid

#136070

Purpose

Acceptor plasmid for even level position 4, lacZ blue-white screening

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

lacZ

Use

Synthetic Biology

Expression

Plant

Available Since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pEn-C1.1

Plasmid

#61479

Purpose

Encodes CRISPR sgRNA for Gateway or conventional cloning of 1-3 sgRNAs into pDe-CAS9 or pCAS9-TPC

Depositor

Holger Puchta

Article

Schiml et al Plant J. 2014 Oct 18. doi: 10.1111/tpj.1

Insert

AtU6-26:sgRNA

Use

CRISPR

Expression

Plant

Promoter

U6-26

Available Since

Sept. 24, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge

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