4,551 results

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pCk1_spacer

Plasmid

#136071

Purpose

Use when making even level constructs (L2,...) if pCk1 position (position 1) has no construct assigned

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

Odd spacer

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available Since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pYPQ239

Plasmid

#108859

Purpose

FnCpf1 Gateway entry plasmid

Depositor

Yiping Qi

Article

Zhong et al Mol Plant. 2018 Jul 2;11(7):999-1002. do

Insert

FnCpf1

Use

CRISPR; Gateway compatible cpf1 entry clone

Tags

NLS

Expression

Plant

Mutation

Cpf1 is rice codon optimized

Available Since

Aug. 27, 2018

Availability

Academic Institutions and Nonprofits only

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pGWB529

Plasmid

#74871

Purpose

Gateway cloning compatible binary vector for C-terminal fusion with TAP (CaMV35S promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Expression

Plant

Promoter

CaMV35S

Available Since

Oct. 18, 2016

Availability

Academic Institutions and Nonprofits only

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pGWB524

Plasmid

#74866

Purpose

Gateway cloning compatible binary vector for N-terminal fusion with GST (CaMV35S promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Expression

Plant

Promoter

CaMV35S

Available Since

Nov. 11, 2016

Availability

Academic Institutions and Nonprofits only

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pMpGWB227

Plasmid

#68618

Purpose

Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

Depositor

Takayuki Kohchi

Article

Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

Type

Empty backbone

Tags

TagRFP

Expression

Plant

Promoter

MpEF1alpha promoter

Available Since

June 24, 2016

Availability

Academic Institutions and Nonprofits only

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pKDH

Plasmid

#67584

Purpose

Gene expression in plants. A promoter and/or reporter can be cloned into this plasmid

Depositor

Indu Maiti

Article

Sahoo et al PLoS One. 2014 Jun 4;9(6):e98988. doi: 1

Type

Empty backbone

Expression

Plant

Promoter

None

Available Since

July 29, 2015

Availability

Academic Institutions and Nonprofits only

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pHub10-His-YFP-PIP2;1

Plasmid

#99386

Purpose

overexpression and isolation of recombinant At-PIP2;1 protein

Depositor

Ning Li

Article

Qing et al Mol Plant. 2016 Jan 4;9(1):158-74. doi:

Insert

PIP2;1 (PIP2A Mustard Weed)

Tags

His and YFP

Expression

Plant

Promoter

2xCaMV35S

Available Since

Aug. 31, 2017

Availability

Academic Institutions and Nonprofits only

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MIGS2.1 (FF570)

Plasmid

#35247

Purpose

Expression of miR173 precursor under UBQ10 promoter; includes miR173 target site after 35S promoter (Basta selection marker)

Depositor

Detlef Weigel

Article

de Felippes et al Plant J. 2011 Dec 28. doi: 10.1111/j.136

Type

Empty backbone

Expression

Plant

Promoter

CaMV 35S

Available Since

March 23, 2012

Availability

Academic Institutions and Nonprofits only

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pYL1300UaUf

Plasmid

#183173

Purpose

Plant binary vector with ccdB flanked by Ua and Uf unique nucleotide sequences.

Depositor

Yao-Guang Liu, Qinlong Zhu

Article

Han et al Plant Biotechnol J. 2022 Feb 18. doi: 10

Type

Empty backbone

Expression

Plant

Available Since

April 26, 2022

Availability

Academic Institutions and Nonprofits only

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pRATIO1112

Plasmid

#141066

Purpose

Gateway cloning compatible dual fluorescent ratiometric binary vector

Depositor

David Nelson

Article

Khosla et al Plant Direct. 2020 Jun 21;4(6):e00231. d

Type

Empty backbone

Tags

mScarlet-I and mVenus

Expression

Plant

Promoter

CaMV 35S promoter

Available Since

Sept. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pRGEB-VirD2-Cas9

Plasmid

#139803

Purpose

Expressing VirD-Cas9 fusion under ubiquitin promoter

Depositor

Magdy Mahfouz

Article

Ali et al Commun Biol. 2020 Jan 23;3(1):44. doi: 1

Insert

VirD2-Cas9

Tags

VirD2-Cas9

Expression

Plant

Promoter

Ubiquitin

Available Since

April 28, 2020

Availability

Academic Institutions and Nonprofits only

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pGEL856

Plasmid

#214256

Purpose

Expression TadDE-NG in rice cells

Depositor

Yong Zhang

Article

Fan et al Nat Commun. 2024 Jun 14;15(1):5103. doi:

Type

Empty backbone

Expression

Plant

Available Since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

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pRGEB34

Plasmid

#158152

Purpose

Construction of inPTG-Cas9 plasmids with a truncated 5'-UTR intron for plant genome editing

Depositor

Kabin Xie

Article

Ding et al Mol Plant. 2018 Apr 2;11(4):542-552. doi

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available Since

Nov. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pGWT35S-MpPHOT-Citrine

Plasmid

#220106

Purpose

Transient expression of MpPHOT-Citrine in plant cells

Depositor

Yutaka Kodama

Article

Yong et al Photochem Photobiol. 2023 Oct 26. doi: 1

Insert

MpPHOT-Citrine

Expression

Plant

Available Since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pGWB443

Plasmid

#74829

Purpose

Gateway cloning compatible binary vector for C-terminal fusion with eCFP (no promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Expression

Plant

Available Since

Sept. 21, 2016

Availability

Academic Institutions and Nonprofits only

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pICSL86900OD

Plasmid

#86178

Purpose

GG compatible single transcript acceptor NPTII

Depositor

Mark Youles

Type

Empty backbone

Expression

Plant

Available Since

Sept. 13, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pRATIO1267

Plasmid

#141301

Purpose

Gateway cloning compatible dual bioluminescent ratiometric binary vector

Depositor

David Nelson

Article

Khosla et al Plant Direct. 2020 Jun 21;4(6):e00231. d

Type

Empty backbone

Tags

gLUC and redLUC

Expression

Plant

Promoter

CaMV 35S promoter

Available Since

Sept. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pDGB3_alpha1-AtUBQ10:GFP-T35S

Plasmid

#211836

Purpose

Plant transient expression cassette for GFP (control) under UBQ10 promoter.

Depositor

Marcela Rojas-Pierce

Article

Huang et al Plant Cell. 2024 Mar 6:koae072. doi: 10.

Insert

GFP control

Use

Synthetic Biology

Tags

GFP

Expression

Plant

Promoter

UBIQUITIN10

Available Since

March 18, 2024

Availability

Academic Institutions and Nonprofits only

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pRATIO4214

Plasmid

#141314

Purpose

Gateway cloning compatible dual fluorescent ratiometric binary vector

Depositor

David Nelson

Article

Khosla et al Plant Direct. 2020 Jun 21;4(6):e00231. d

Type

Empty backbone

Tags

mCerulean and mScarlet-I

Expression

Plant

Promoter

UBQ10 promoter

Available Since

Sept. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pYPQ262m3

Plasmid

#149556

Purpose

Gateway entry clone for CRISPR-iSpyMacCas9 ABE7.10 A-G base editing at NAAR PAMs

Depositor

Yiping Qi

Article

Sretenovic et al Plant Commun. 2020 Jul 22;2(2):100101. d

Insert

ecTadAwt-ecTadAmut-ziSpyMacCas9(D10A)

Use

CRISPR

Expression

Plant

Mutation

D10A, R221K, N394K, Protospacer interacting domai…

Available Since

July 20, 2020

Availability

Academic Institutions and Nonprofits only

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pYPQ133C2.0

Plasmid

#99893

Purpose

Golden Gate entry vector to express the 3rd gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under OsU6 promoter

Depositor

Yiping Qi

Article

Lowder et al Mol Plant. 2017 Nov 29. pii: S1674-2052(

Type

Empty backbone

Use

CRISPR

Expression

Plant

Promoter

OsU6

Available Since

March 8, 2018

Availability

Academic Institutions and Nonprofits only

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pEPOR1CB0013

Plasmid

#117549

Purpose

Level 1 Golden Gate Cassette: LbCas12a expression cassette for dicotyledonous plants

Depositor

Nicola Patron

Article

Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

Insert

2x35S+5'UTR OMEGA (pICH51288) + LbCas12a (with stop codon) (pEPOR0SP0007)+ Nos (pICH41421)

Expression

Plant

Promoter

2x35S+5'UTR OMEGA (pICH51288)

Available Since

Nov. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pNC-BiFC-ED1

Plasmid

#193426

Purpose

Nimble cloning-compatible vector for plant BiFC

Depositor

Pu Yan

Article

Yan et al Plant Commun. 2023 Mar 13;4(2):100471. d

Type

Empty backbone

Expression

Plant

Available Since

Dec. 19, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMB-PMr

Plasmid

#61181

Purpose

Fluorescent reporter for plasma membrane AtPIP2a-mCherry expressed under MtBCP1 promoter

Depositor

Maria Harrison

Article

Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

Insert

AtPIP2a

Tags

mCherry

Expression

Plant

Promoter

MtBCP1

Available Since

March 30, 2015

Availability

Academic Institutions and Nonprofits only

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pMAP L1A

Plasmid

#153343

Purpose

Mobius Assembly cloning and expression vector for plants with high-copy number origin of replication

Depositor

Naomi Nakayama

Article

Andreou et al bioRxiv, March 31, 2021

Type

Empty backbone

Expression

Plant

Available Since

Nov. 30, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pIAA5::LUC

Plasmid

#141169

Purpose

Luminescent reporter for auxin signaling

Depositor

Vardis Ntoukakis, Patrick Schäfer

Article

Lehmann et al PLoS One. 2020 Jun 4;15(6):e0234154. doi

Insert

AtIAA5 promoter

Use

Luciferase

Expression

Plant

Promoter

AtIAA5, At1g15580

Available Since

Aug. 7, 2020

Availability

Academic Institutions and Nonprofits only

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AL13Rb-GAPC2

Plasmid

#161009

Purpose

PP7-tagged reporter driven by the Arabidopsis GAPC2 promoter

Depositor

Krishna Niyogi

Article

Alamos et al Nat Plants. 2021 Aug;7(8):1037-1049. doi

Insert

PP7 and H2B-mScarlet

Expression

Plant

Available Since

Dec. 8, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
p231-pOsAct1::hpt-pZmUbi::BdPetC-p35S::mTurquoise

Plasmid

#129650

Purpose

Rieske FeS overexpression

Depositor

Susanne von Caemmerer

Article

Ermakova et al Commun Biol. 2019 Aug 16;2:314. doi: 10.

Insert

CDS of PetC gene encoding for the Rieske FeS subunit

Expression

Plant

Mutation

Codon modified for Golden Gate

Promoter

pZmUbi

Available Since

Oct. 30, 2019

Availability

Academic Institutions and Nonprofits only

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Raichu-OsRac1 CA

Plasmid

#133264

Purpose

Constitutively-active (CA) mutant of OsRac1; Acts as a positive control for small GTPases activation assays.

Depositor

Yoji Kawano

Article

Wong et al Plant Methods. 2018 Jul 7;14:56. doi: 10

Insert

OsRac1(G19V) (LOC4325879 Oryza sativa)

Tags

CFP-lipid and Venus-CRIB

Expression

Plant

Mutation

OsRac1 G19V

Promoter

Ubi

Available Since

Jan. 24, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
Raichu-OsRac1 DN

Plasmid

#133263

Purpose

Dominant-negative (DN) mutant of OsRac1; Acts as a negative control for small GTPases activation assays

Depositor

Yoji Kawano

Article

Wong et al Plant Methods. 2018 Jul 7;14:56. doi: 10

Insert

OsRac1(T24N) (LOC4325879 Oryza sativa)

Tags

CFP-lipid and Venus-CRIB

Expression

Plant

Mutation

OsRac1 T24N

Promoter

Ubi

Available Since

Jan. 24, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
PEP102E

Plasmid

#97388

Purpose

binary construct of nuclear-targeted sfGFP1-10 for plant expression

Depositor

Savithramma Dinesh-Kumar

Article

Park et al Plant Cell. 2017 Jul;29(7):1571-1584. do

Insert

sfGFP1-10OPT

Tags

Nuclear target

Expression

Plant

Available Since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pMpGWB136

Plasmid

#68590

Purpose

Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

Depositor

Takayuki Kohchi

Article

Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

Type

Empty backbone

Tags

TagRFP

Expression

Plant

Promoter

MpEF1alpha promoter

Available Since

June 24, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pMDC32-SEIPIN1

Plasmid

#96973

Purpose

Express Arabidopsis SEIPIN1 gene in plants.

Depositor

Kent Chapman

Article

Cai et al Plant Cell. 2015 Sep;27(9):2616-36. doi:

Insert

SEIPIN1 (AT5G16460 Mustard Weed)

Expression

Plant

Promoter

35S

Available Since

Aug. 9, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pMC-1-EXOB-11

Plasmid

#197688

Purpose

Phytobrick (MoClo) Level 0 Part

Depositor

Daniel Voytas

Article

Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

Insert

EXOB enhancer-blocking insulator

Expression

Plant

Available Since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pDGE670

Plasmid

#153236

Purpose

Recipient plant transformation vector containing selection markers and a Cas9 expression cassette, but lacking guide RNAs

Depositor

Sylvestre Marillonnet

Article

Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available Since

Sept. 9, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMB-PDESr

Plasmid

#61207

Purpose

Fluorescent reporter for plasmodesmata AtPDLP1-mCherry expressed under AtUBQ10 promoter

Depositor

Maria Harrison

Article

Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

Insert

AtPDLP1

Tags

mCherry

Expression

Plant

Promoter

MtBCP1

Available Since

Feb. 12, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pSB63 - pL0_fLUC-I (CDS1)

Plasmid

#123180

Purpose

Golden Gate (MoClo; CDS1) compatible luciferase gene from Photinuspyralis with an intron for transient and stable expression in plants

Depositor

Erin Cram, Carolyn Lee-Parsons

Article

Mortensen et al Front Plant Sci. 2019 Jun 11;10:755. doi

Insert

Luciferase gene from Photinuspyralis with intron from U5 small nuclear ribonucleoprotein component (Arabidopsis thaliana)

Use

Luciferase; Part for plant expression

Expression

Plant

Mutation

No BpiI and BsaI sites

Available Since

May 29, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
PEP107E

Plasmid

#97393

Purpose

binary construct of Golgi-targeted sfGFP1-10 for plant expression

Depositor

Savithramma Dinesh-Kumar

Article

Park et al Plant Cell. 2017 Jul;29(7):1571-1584. do

Insert

sfGFP1-10OPT

Tags

Golgi target

Expression

Plant

Available Since

Sept. 25, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pCLV3Cas-LT0

Plasmid

#210548

Purpose

Binary vector expressing the Lineage Tracing system. Cas9 expression driven by CLV3 promoter. LT0 reporter

Depositor

Ortrun Mittelsten Scheid

Article

Dona et al Plant J. 2023 Jul 5. doi: 10.1111/tpj.16

Insert

CLV3p::spCas9::PEA3At::U6p::AAVS1gRNA::pHTR5::LT0::mCherrySSM

Expression

Plant

Available Since

April 22, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
LT0

Plasmid

#210551

Purpose

Binary vector expressing the Lineage Tracing system. No Cas9 control expression cassette. LT0 reporter

Depositor

Ortrun Mittelsten Scheid

Article

Dona et al Plant J. 2023 Jul 5. doi: 10.1111/tpj.16

Insert

pU6::AAVS1gRNA::LT0::mCherrySSM

Expression

Plant

Available Since

March 25, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge

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4,551 results