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4,457 results

Showing: 1481 - 1520 of 4457 results

  1. pTUU

    Plasmid
    #159749
    Purpose
    Ubiquitin promoter-controlled expression of 3×FLAG-NLS-zCas9-NLS. Contains gRNA scaffold for insertion of target sequence (U6-26 promoter). Selection of transgenic seeds by mTomato fluoresce.
    Depositor
    Wolfgang Lukowitz
    Article
    Angulo et al bioRxiv
    Insert
    gRNA scaffold
    Use
    CRISPR
    Expression
    Plant
    Available Since
    Oct. 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    159749-plasmid-map-sequence-id-313818

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  2. pSW683 - pAHP6>>

    Plasmid
    #115998
    Purpose
    destination vector for GreenGate cloning method, contains 2x a set of modules from A-F, "Driver line", tissues-specific promoter, Dex-inducible mTurquoise2 expression
    Depositor
    Sebastien Wolf
    Article
    Schurholz et al Plant Physiol. 2018 Jul 19. pii: pp.18.0
    Insert
    pAHP6:GR-LhG4:tRBCS:F-H adapter-H-A adapter::pOp4:SP(ER)-mTurquoise2-HDEL:tUBQ10::SulfR
    Tags
    HDEL and SP(ER)
    Expression
    Plant
    Available Since
    Dec. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    115998-plasmid-map-sequence-id-227486

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  6. pMDC32B-AtMIR390a-NbSu-2

    Plasmid
    #213400
    Purpose
    Plant expression vector (2x35S) for expressing an amiRNA against Nicotiana benthamiana SULFUR gene from AtMIR390a precursor
    Depositor
    Alberto Carbonell
    Article
    Cisneros et al Plant J. 2022 May;110(4):1166-1181. doi:
    Insert
    Arabidopsis MIR390a precursor including amiRNA/amiRNA* sequences for silencing N. benthamiana SULFUR gene
    Expression
    Plant
    Promoter
    2x35S
    Available Since
    Feb. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    213400-plasmid-map-sequence-id-422344

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  13. pYPQ265G2

    Plasmid
    #164723
    Purpose
    Gateway entry clone (attL1 & attR5) A3A/Y130F-iSpyMacCas9-CBE_V01 for C-T base editing
    Depositor
    Yiping Qi
    Article
    Ren et al Plant Biotechnol J. 2021 May 27. doi: 10
    Insert
    A3A(Y130F)-ziSpyMac(D10A)-UGI)
    Use
    CRISPR; Gateway compatible a3a/y130f-ispymaccas9-…
    Expression
    Plant
    Available Since
    Aug. 31, 2021
    Availability
    Academic Institutions and Nonprofits only
    164723-plasmid-map-sequence-id-341525

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  16. pdCas9 (GB1079)

    Plasmid
    #75399
    Purpose
    Provides the human codon optimized CDS of Cas9 protein with mutated (D10A, H840A) and inactivated catalytic domains as a level 0 GoldenBraid part for C-terminal fusions
    Depositor
    Diego Orzaez
    Article
    Vazquez-Vilar et al Plant Methods. 2016 Feb 1;12:10. doi: 10
    Insert
    Cas9 coding region with mutated (D10A, H840A) and inactivated catalytic domains (human codon optimised)
    Use
    CRISPR and Synthetic Biology
    Expression
    Plant
    Available Since
    Aug. 5, 2016
    Availability
    Academic Institutions and Nonprofits only
    75399-plasmid-map-sequence-id-151817

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  22. A3A-PBE-ΔUGI

    Plasmid
    #119770
    Purpose
    Conversion of C to T in wheat plants or monocotyledons protoplasts without uracil glycosylase inhibitor (UGI)
    Depositor
    Caixia Gao
    Article
    Zong et al Nat Biotechnol. 2018 Oct 1. pii: nbt.426
    Insert
    APOBEC3A-nCas9-ΔUGI-NLS
    Tags
    NLS
    Expression
    Plant
    Mutation
    nCas9 has a mutation D10A in wild type SpCas9
    Promoter
    maize Ubiquitin-1
    Available Since
    Dec. 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    119770-plasmid-map-sequence-id-232089

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  24. P303-FBP_2

    Plasmid
    #139693
    Purpose
    T-DNA encoding Fungal Bioluminescent Pathway Components, complete pathway, without recycling gene CPH
    Depositor
    Daniel Voytas
    Article
    Khakhar et al Elife. 2020 Mar 25;9. pii: 52786. doi: 1
    Insert
    p35S_Short_TMV 5':AsNpga:tAtug7, pCmYLCV:NnH3H:AtuOCS, pCmYLCV:NnHisps:tHsp, p35S:NnLuz:tAtuOcs
    Use
    Luciferase
    Expression
    Plant
    Promoter
    various
    Available Since
    May 29, 2020
    Availability
    Academic Institutions and Nonprofits only
    139693-plasmid-map-sequence-id-296189

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  28. pDGB3 alpha1 U6-26:gRNA 4 (Pnos):2.1 aptamer (GB1724)

    Plasmid
    #160621
    Purpose
    Target for the Nopaline Synthetase Promoter with the MS2 recognition 2.1 loop in position3' in the scaffold
    Depositor
    Diego Orzaez
    Article
    Selma et al Plant Biotechnol J. 2019 Sep;17(9):1703-
    Insert
    U6-26:gRNA 4 (pNos): 2.1 aptamer
    Use
    CRISPR and Synthetic Biology
    Expression
    Plant
    Mutation
    BsaI and BsmBI sites removed
    Promoter
    U6-26
    Available Since
    Jan. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    160621-plasmid-map-sequence-id-322006

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  36. pFGC-I2Cas9

    Plasmid
    #173158
    Purpose
    Expresses Cas9 in dividing Arabidopsis cells. Contains dsRED and Basta for fast transformant selection.
    Depositor
    Piotr Ziolkowski
    Article
    Bieluszewski et al Nat Commun. 2022 Jan 12;13(1):277. doi:
    Inserts
    hSpCas9
    pNAP:dsRED:tNOS
    pMAS:BAR:tMAS
    ICU2 upstream regulatory region
    Tags
    3xFLAG-NLS and NLS
    Expression
    Plant
    Mutation
    Contains a D169N mutation with no visible effect …
    Available Since
    June 3, 2022
    Availability
    Academic Institutions and Nonprofits only
    173158-plasmid-map-sequence-id-358594

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  37. pFH113

    Plasmid
    #128210
    Purpose
    improved sgRNA backbone for tRNA-gRNA array, Position 1, combine with TaU3 promoter module (pFH31)
    Depositor
    Vladimir Nekrasov
    Article
    Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d
    Insert
    improved sgRNA backbone for tRNA-gRNA array, Position 1, combine with TaU3 promoter module (pFH31)
    Use
    CRISPR and Synthetic Biology
    Expression
    Plant
    Available Since
    Aug. 19, 2019
    Availability
    Academic Institutions and Nonprofits only
    128210-plasmid-map-sequence-id-252904

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Showing: 1481 - 1520 of 4457 results