4,233 results

1. pYPQ230-E795L

   Plasmid

   #176890

   Purpose

   Gateway entry plasmid (attL1 & attR5) expressing LbCas12a with E795L mutation, without promoter

   Depositor

   Yiping Qi

   Article

   Zhang et al Front Genome Ed. 2022 Jan 31;4:780238. d

   Insert

   LbCas12a-E795L
   

   Use

   CRISPR; Gateway compatible lbcpf1 entry clone

   Tags

   Nucleoplasmin NLS and SV40 NLS

   Expression

   Plant

   Mutation

   Promoter

   Available since

   April 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDGE1108

   Plasmid

   #202772

   Purpose

   CRISPR-KO in Arabidopsis thaliana

   Depositor

   Johannes Stuttmann

   Article

   Ordon et al Plant Methods. 2023 Mar 28;19(1):30. doi

   Inserts

   FAST marker
   pRPS5a:zCas9_ttriple
   ccdB cassette (BsaI-excisable)
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Aug. 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pH-Ubi-miniSdd7-nCas9-PBE

   Plasmid

   #204856

   Purpose

   For cytosine base editing using miniSdd7 in monocotyledon plants (Agrobacterium-mediated transformation)

   Depositor

   Caixia Gao

   Article

   Huang et al Cell. 2023 Jul 20;186(15):3182-3195.e14.

   Insert

   miniSdd7-nSpCas9(D10A)-UGI
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   D10A in SpCas9

   Promoter

   ZmUbi-1, OsU3, 35S

   Available since

   Aug. 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pYPQ233 (dLbCpf1-SRDX)

   Plasmid

   #86211

   Purpose

   Deactivated LbCpf1-SRDX repressor fusion; transcriptional repressor dCpf1 Gateway entry plasmid

   Depositor

   Yiping Qi

   Article

   Tang et al Nat Plants. 2017 Feb 17;3:17018. doi: 10

   Insert

   LbCpf1
   

   Use

   CRISPR

   Tags

   5' and 3' NLS and SRDX repressor

   Expression

   Plant

   Mutation

   Promoter

   Available since

   April 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGG-TurboID

   Plasmid

   #209391

   Purpose

   Transiently expressing TurboID in planta

   Depositor

   Rosa Lozano-Durán

   Article

   Tan et al J Integr Plant Biol. 2024 Jan 31. doi: 1

   Insert

   3XHA-TurboID
   

   Use

   Tags

   Expression

   Plant

   Mutation

   TurboID gene sequence C249A

   Promoter

   Cauliflower mosaic virus (CaMV) 35S promoter

   Available since

   Jan. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDEST17-UnaG

   Plasmid

   #203485

   Purpose

   For recombinant protein production in Escherichia coli. His-tagged UnaG under the control of the T7 promoter.

   Depositor

   Yutaka Kodama

   Article

   Ishikawa et al Sci Adv. 2023 Jun 9;9(23):eadh4787. doi:

   Insert

   UnaG
   

   Use

   Tags

   Expression

   Bacterial and Plant

   Mutation

   Promoter

   Available since

   Aug. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pECNUS_MP02

   Plasmid

   #194351

   Purpose

   CRISPR-Cas9 multiplexing acceptor clone with BsaI cutter

   Depositor

   Eunyoung Chae

   Article

   Lee et al Nucleic Acids Res. 2023 Mar 15:gkad142.

   Insert

   pcoCas9
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   RPS5A

   Available since

   March 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. UBQ10:sXVE:(MCS)-S11

   Plasmid

   #108179

   Purpose

   Binary vectors used for the inducible expression of tripartite split-sfGFP components in plants

   Depositor

   Tzu-Yin Liu

   Article

   Liu et al Plant J. 2018 Feb 16. doi: 10.1111/tpj.1

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   Promoter

   UBQ10:sXVE:

   Available since

   April 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pFG7m34GW

   Plasmid

   #133747

   Purpose

   Three-fragment Multisite Gateway vector construction for green fluorescent seed selection in Arabidopsis thaliana

   Depositor

   Niko Geldner

   Article

   Wang et al Nat Plants. 2020 Jun 29. pii: 10.1038/s4

   Type

   Empty backbone

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Feb. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pZmUBQ:GW

    Plasmid

    #132358

    Purpose

    Expression of CDS from the Zea mays ubiquitin promotor

    Depositor

    Paul Schulze-Lefert

    Article

    Saur et al Plant Methods. 2019 Oct 24;15:118. doi:

    Type

    Empty backbone

    Use

    Tags

    YFP

    Expression

    Plant

    Mutation

    Promoter

    pZmUBQ

    Available since

    Oct. 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pTRANS_213

    Plasmid

    #91112

    Purpose

    Transformation, Type: T-DNA, Plant Selection: 2x35S:hpt II, Viral Replication: WDV

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    35S

    Available since

    Sept. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pPPEM

    Plasmid

    #162467

    Purpose

    Destination Expression vector for monocot plant primer editing

    Depositor

    Adam Bogdanove

    Article

    Wang et al Front Genome Ed. 2021 Mar 4;3:617553. do

    Insert

    Cas9H840A
    

    Use

    Tags

    MLV reverse transcriptase

    Expression

    Plant

    Mutation

    Change histidine 840 to Alanine

    Promoter

    maize Ubi

    Available since

    June 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pARSCL504

    Plasmid

    #198636

    Purpose

    Expresses a transgene encoding RUBY

    Depositor

    Shaun Curtin

    Insert

    35S:Ω:Ruby
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    June 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDGB3_alpha1-pAtUBQ10:HA-LRR-NELC492A-mcherry-Tnos

    Plasmid

    #211843

    Purpose

    Plant transient expression cassette for the E3-DART ligase under a UBQ10 promoter.

    Depositor

    Marcela Rojas-Pierce

    Article

    Huang et al Plant Cell. 2024 Mar 6:koae072. doi: 10.

    Insert

    LRR-NEL
    

    Use

    Synthetic Biology

    Tags

    HA and mCherry

    Expression

    Plant

    Mutation

    C492A

    Promoter

    UBIQUITIN10

    Available since

    March 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pYPQ146-ZmUbi-tRNA

    Plasmid

    #158404

    Purpose

    Gateway entry clone and Golden Gate recipient for pYPQ131-tRNA2.0 to pYPQ136-tRNA2.0; assembly of 6 gRNAs

    Depositor

    Yiping Qi

    Article

    Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Maize ubiquitin 1

    Available since

    July 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pYPQ144-ZmUbi-tRNA

    Plasmid

    #158402

    Purpose

    Gateway entry clone and Golden Gate recipient for pYPQ131-tRNA2.0 to pYPQ134-tRNA2.0; assembly of 4 gRNAs

    Depositor

    Yiping Qi

    Article

    Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Maize ubiquitin 1

    Available since

    June 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pH-dCas9-PBE

    Plasmid

    #98162

    Purpose

    Expresses CRISPR base editor in rice

    Depositor

    Caixia Gao

    Article

    Zong et al Nat Biotechnol. 2017 May;35(5):438-440.

    Insert

    NLS-APOBEC1-XTEN-dCas9-UGI-NLS
    

    Use

    Rice

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Ubi

    Available since

    July 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDL691

    Plasmid

    #231166

    Purpose

    T-DNA encoding TRV2 with ipt and mobile gRNAs targeting SlUGT75C1 and SlHAIRLESS

    Depositor

    Daniel Voytas

    Article

    Liu et al Proc Natl Acad Sci U S A. 2024 Sep 24;12

    Insert

    ipt and mobile gRNAs targeting SlUGT75C1 and SlHAIRLESS
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    PEBV sub-genomic

    Available since

    Jan. 15, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDB1alpha11:empty

    Plasmid

    #106207

    Purpose

    GoldenBraid level 1 Alpha plant expression vector.

    Depositor

    Tomáš Moravec

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    June 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pUBQ10-mNeonGreen-GW

    Plasmid

    #209352

    Purpose

    Gateway cloning compatible binary vector for agrobacterium mediated transformation. Arabidopsis UBQ10 promoter expression of N-terminal mNeonGreen fusion protein.

    Depositor

    Lucia Strader

    Article

    Allen et al Res Sq. 2023 Nov 7:rs.3.rs-3558280. doi:

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Feb. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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