4,550 results
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Plasmid#99894PurposeGolden Gate entry vector to express the 3rd gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under OsU3 promoterDepositorTypeEmpty backboneUseCRISPRExpressionPlantPromoterOsU3Available SinceMarch 8, 2018AvailabilityAcademic Institutions and Nonprofits only
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pYPQ131D2.0
Plasmid#100044PurposeGolden Gate entry vector to express the 1st gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under OsU3 promoterDepositorTypeEmpty backboneUseCRISPRExpressionPlantPromoterOsU3Available SinceMarch 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
P16ΔS:(MCS)-3xHA-S11
Plasmid#108185PurposeBinary vectors used for the constitutive expression of tripartite split-sfGFP components (with a 3xHA tag) in plantsDepositorTypeEmpty backboneUseSynthetic BiologyExpressionPlantPromoterP16ΔSAvailable SinceMay 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGWB428
Plasmid#74822PurposeGateway cloning compatible binary vector for C-terminal fusion with TAP (no promoter).DepositorTypeEmpty backboneExpressionPlantAvailable SinceJuly 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
gRD21A-mRFP
Plasmid#181959PurposeRD21A protein tagged with mRFP, under control of native promoterDepositorInsertRD21A genomic sequence including 2kb upstream of the ATG
TagsmRFPExpressionPlantPromoterRD21A nativeAvailable SinceMarch 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFGC5941-PacI-ath-STTM403
Plasmid#84128Purposetarget miRNA403 for destruction in Arabidopsis thalianaDepositorInsertSTTM403
ExpressionPlantPromoter2x35SAvailable SinceNov. 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
lvl0 UBQ4p
Plasmid#153366PurposePlant promoterDepositorInsertUBQ4 promoter
ExpressionPlantAvailable SinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
lvl0 TCTPp
Plasmid#153368PurposePlant promoterDepositorInsertTCTP promoter
ExpressionPlantAvailable SinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
lvl0 OCSp
Plasmid#153367PurposeAgrobacterium promoter for plant expressionDepositorInsertOCS promoter
ExpressionPlantAvailable SinceOct. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
lvl0 MASt
Plasmid#153381PurposeAgrobacterium terminator for plant expressionDepositorInsertMAS terminator
ExpressionPlantAvailable SinceOct. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAtCas9-H840A_1
Plasmid#91162PurposeCas9 only expression, Cas9 Type: AtCas9_H840A (nickase), Plant Selection: 2x35S:hpt IIDepositorInsertAtCas9_H840A (nickase)
UseCRISPRExpressionPlantMutationH840AAvailable SinceSept. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTaCas9-H840A_6
Plasmid#91173PurposeCas9 only expression, Cas9 Type: TaCas9_H840A (nickase), Plant Selection: PvUbi2:barDepositorInsertTaCas9_H840A (nickase)
UseCRISPRExpressionPlantMutationH840AAvailable SinceJuly 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
NSm-GFP pZK538
Plasmid#118012Purposeexpresses NSm-GFP and mCherry-HDEL. Used for visualization of cell-to-cell movement of NSm. NSm can be replaced with gene of interest via HiFi DNA assembly cloning (NEB).DepositorArticleInsertsNSm
mCherry
TagsEGFP and HDELExpressionBacterial and PlantPromoter35SAvailable SinceJuly 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFH88
Plasmid#128201PurposesgRNA backbone for SaCas9, combine with TaU3 promoter module (pFH33)DepositorInsertsgRNA backbone for SaCas9, combine with TaU3 promoter module (pFH33)
UseCRISPR and Synthetic BiologyExpressionPlantAvailable SinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pVL44 - pVND7>>
Plasmid#116010Purposedestination vector for GreenGate cloning method, contains 2x a set of modules from A-F, "Driver line", tissues-specific promoter, Dex-inducible mTurquoise2 expressionDepositorInsertpVND7:GR-LhG4:tVND7:F-H adapter-H-A adapter::pOp4:SP(ER)-mTurquoise2-HDEL:tUBQ10::SulfR
TagsHDEL and SP(ER)ExpressionPlantAvailable SinceDec. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB216
Plasmid#68607PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneTagsNuclear localization signal-tagged tdTomato (tdTo…ExpressionPlantAvailable SinceJune 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
CTAG_linker-eGFP
Plasmid#136098PurposeFP for C-term fusion with CDS12 without linkerDepositorInsertCTAG_AlaSerGly10xAlaGlySer_eGFP
UseSynthetic BiologyExpressionPlantMutationBsaI/ SapI domesticatedAvailable SinceNov. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
CTAG_linker-mTurquoise2
Plasmid#136100PurposeFP for C-term fusion with CDS12 without linkerDepositorInsertCTAG_AlaSerGly10xAlaGlySer_mTurquoise2
UseSynthetic BiologyExpressionPlantMutationBsaI/ SapI domesticatedAvailable SinceNov. 30, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
CDS_GUS
Plasmid#136084PurposeFor histochemical _-glucuronidase activity assays. This GUS CDS does not have an intron.DepositorInsertCDS_GUS
UseSynthetic BiologyExpressionPlantMutationBsaI/ SapI domesticatedAvailable SinceNov. 30, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
CDS12_eGFP-linker
Plasmid#136086PurposeFP for N-term fusion with a CTAG without linkerDepositorInsertCDS12_eGFP(noStop)-SerGly10xAlaSer
UseSynthetic BiologyExpressionPlantMutationBsaI/ SapI domesticatedAvailable SinceNov. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
CDS12_mScarletI-linker
Plasmid#136088PurposeFP for N-term fusion with a CTAG without linkerDepositorInsertCDS12_mScarletI(noStop)-SerGly10xAlaSer
UseSynthetic BiologyExpressionPlantMutationBsaI/ SapI domesticatedAvailable SinceNov. 30, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
CDS12_mScarlet-I
Plasmid#136087PurposeFP for N-term fusion with a CTAGDepositorInsertCDS12_mScarletI(noStop)
UseSynthetic BiologyExpressionPlantMutationBsaI/ SapI domesticatedAvailable SinceNov. 30, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
cp-DdCBE G1333-C
Plasmid#174557PurposeChloroplast-targeting DdCBE (split DddA, G1333-C) Golden Gate destination vectorDepositorInsertDdCBE G1333-C
TagsFLAGExpressionPlantPromoterPcUbiAvailable SinceOct. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
MIGS4.2 (FF575)
Plasmid#35252DepositorTypeEmpty backboneExpressionPlantAvailable SinceMarch 23, 2012AvailabilityAcademic Institutions and Nonprofits only -
pBGN155-MXMT
Plasmid#79817PurposeGFP(S65T)-based BiFC vector for plants (Positive control: MXMT)DepositorInsertCaMXMT1
UseReporter plasmidTagsGN155/GFP (1–155)ExpressionPlantPromoterCaMV 35S promoter and CaMV35SAvailable SinceAug. 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
pYPQ141-ZmUbi-RZ-Aa3.8.4
Plasmid#136375PurposeGateway entry clone for AaCas12b sgRNA expression under ZmUbi promoter with ribozyme processing; sgRNA scaffold 3.8 with MS2 at the third stem loop is usedDepositorTypeEmpty backboneUseCRISPRExpressionPlantAvailable SinceFeb. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFGC5941-PacI-ath-STTM829.2
Plasmid#84158Purposetarget miRNA829.2 for destruction in Arabidopsis thalianaDepositorInsertSTTM829.2
ExpressionPlantPromoter2x35SAvailable SinceDec. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSB165 - pL1R3_pMAS::rLUC-I::tMAS
Plasmid#123198Purposebinary plant vector for transient expression of a Renilla luciferase (rLUC, with intron)DepositorInsertpMAS::rLUC-I::tMAS
UseLuciferaseExpressionPlantAvailable SinceMay 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB423
Plasmid#68688PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneTagsTriple repeats of Citrine (3xCitrine)ExpressionPlantAvailable SinceJune 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTX208
Plasmid#89268PurposeExpress STU Cas9 with Maize Ubiquitin1 promoter in rice targeting OsYSA gene, OsYSA-gRNA01 and OsYSA-gRNA02DepositorInsertOsYSA-gRNA01 and OsYSA-gRNA02
UseCRISPRTagsSV40 NLSExpressionPlantPromoterMaize Ubiquitin1 promoterAvailable SinceMay 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
INCTbiosyn-pAct-AtINT2
Plasmid#127529PurposePlasmid encodes A. thaliana codon optimized Integrase 2.DepositorInsertIntegrase 2 coding sequence codon optimized for A. thaliana expression.
ExpressionPlantAvailable SinceMay 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
INCTbiosyn-pAct-AtINT5
Plasmid#127531PurposePlasmid encodes A. thaliana codon optimized Integrase 5.DepositorInsertIntegrase 5 coding sequence codon optimized for A. thaliana expression.
ExpressionPlantAvailable SinceMay 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
INCTbiosyn-pAct-AtINT7
Plasmid#127532PurposePlasmid encodes A. thaliana codon optimized Integrase 7.DepositorInsertIntegrase 7 coding sequence codon optimized for A. thaliana expression.
ExpressionPlantAvailable SinceMay 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
INCTbiosyn-pAct-AtINT9
Plasmid#127533PurposePlasmid encodes A. thaliana codon optimized Integrase 9.DepositorInsertIntegrase 9 coding sequence codon optimized for A. thaliana expression.
ExpressionPlantAvailable SinceMay 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGWB14-LYX001
Plasmid#126219PurposeWith a Nicotiana benthamiana transcription factor in PGWB14 backbone, for Agrobacterium mediated transient or stable expression corresponding transcription factor fused with a C-terminal HA tag.DepositorInsertNiben101Scf00149g02001.1
TagsHAExpressionPlantPromoterCaMV 35SAvailable SinceJune 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pYPQ284-v1
Plasmid#138118PurposeGateway entry plasmid (attL1 & attR5) expressing rice codon optimized Mb2Cas12a with D172R mutation, without promoterDepositorInsertMb2Cas12a v1
UseCRISPR; Gateway compatible cas12a entry cloneTagsNLSExpressionPlantMutationD172RAvailable SinceMarch 31, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLEELA
Plasmid#79752Purposebinary DEST vector for plant expressionDepositorTypeEmpty backboneExpressionPlantPromoter2xPro35SAvailable SinceSept. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDGB1a2R+pNOS_Red-F_NOSt
Plasmid#170888PurposeGoldenBraid Transcriptional Unit - pNOS_Red-F_NOSt Reverse orientationDepositorInsertRed-F
UseLuciferase and Synthetic BiologyExpressionPlantMutationp.S286Y Red mutantPromoterpNOSAvailable SinceAug. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
LjUBQ:MtGLV6:T35s
Plasmid#164741PurposeOverexpression of Peptide coding gene (Binary vector)DepositorInsertMedtr3g095180.1
TagsTerminator 35SExpressionPlantPromoterLotus japonicus UbiquitinAvailable SinceDec. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
LjUBQ:MtGLV2:T35s
Plasmid#164740PurposeOverexpression of Peptide coding gene (Binary vector)DepositorInsertMedtr5g012400.1
TagsTerminator 35SExpressionPlantPromoterLotus japonicus UbiquitinAvailable SinceDec. 13, 2021AvailabilityAcademic Institutions and Nonprofits only