4,550 results
-
Plasmid#174778PurposeIn planta gene expressionDepositorInsertGG_NbZAR1syn-eGFP, Kmr plant selection marker
TagseGFPExpressionPlantMutationWTAvailable SinceOct. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pZA67
Plasmid#174780PurposeIn planta gene expressionDepositorInsertGG_NbZAR1syn-L17E-eGFP, Kmr plant selection marker
TagseGFPExpressionPlantMutationL17EAvailable SinceOct. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDGB3_alpha1 P35S:SV40-AcrIIA4_CONb:Tnos (GB3344)
Plasmid#160635PurposeTU for SV40-AcrIIA4 protein Codon optimized for N. benthamiana expression under the regulation of the 35S promoter and Tnos terminatorDepositorInsertAcrIIA4
UseSynthetic BiologyExpressionPlantMutationBsaI and BsmBI sites removedPromoter35SAvailable SinceSept. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRATIO2251
Plasmid#141305PurposeGateway cloning compatible ratiometric binary vectorDepositorTypeEmpty backboneTagsFirefly Luciferase or LUC2 and mScarlet-IExpressionPlantPromoterUBQ10 promoterAvailable SinceSept. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB106-OEP7(1–50)
Plasmid#100602PurposeBinary vector for expression of Citrine-fused AtOEP7(1-50) (OEP7(1–50)-Citrine) to visualize chloroplast outer envelope membrane in plantsDepositorInsertOEP7(1–50)-Citrine (OEP7 Mustard Weed)
ExpressionPlantAvailable SinceFeb. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTaCas9_5
Plasmid#91168PurposeCas9 only expression, Cas9 Type: TaCas9, Plant Selection: PvUbi2:hpt IIDepositorInsertTaCas9
UseCRISPRExpressionPlantAvailable SinceJuly 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAtCas9-H840A_2
Plasmid#91163PurposeCas9 only expression, Cas9 Type: AtCas9_H840A (nickase), Plant Selection: 2x35S:npt IIDepositorInsertAtCas9_H840A (nickase)
UseCRISPRExpressionPlantMutationH840AAvailable SinceSept. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pUBQ10::ZF1CACTA1_3xFLAG_TET1cd
Plasmid#106434PurposepUBQ10 driven Zinc Finger human TET1 catalytic domain fusion that targets the CACTA1 promoter in arabidopsisDepositorInsertTET1 tet methylcytosine dioxygenase 1 (TET1 Synthetic, Human)
UseSynthetic BiologyTags3xFLAGExpressionPlantAvailable SinceMarch 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDGB3_Omega1_Pnos:ER:lexABD:GAL4AD:T35s-OplexA:mini35S:phi31:Tnos (GB1677)
Plasmid#160641PurposeModule for estradiol-inducible exp of the PhiC31 integrase gene. Includes TU for constitutive exp of an estradiol-inducible transcription activator and TU for estradiol-inducible exp of PhiC31.DepositorInsertERLexABDGal4AD / PhiC31
UseSynthetic BiologyExpressionPlantMutationBsaI and BsmBI sites removedPromoterPnosAvailable SinceJan. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pG20_LUC2_Hyg
Plasmid#159707PurposepGREEN-based cloning construct for expression in plantaDepositorTypeEmpty backboneTagsluciferaseExpressionPlantAvailable SinceOct. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pG20_GUS_Blp
Plasmid#159706PurposepGREEN-based cloning construct for expression in plantaDepositorTypeEmpty backboneTagsGUSExpressionPlantAvailable SinceOct. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEE386
Plasmid#149284PurposeT-DNA encoding TRV2 with gRNA targeting NbAGDepositorInsertp35S:TRV2_NbAGsgRNA1:tNos
ExpressionPlantPromoter35SAvailable SinceJuly 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEE388
Plasmid#149286PurposeT-DNA encoding TRV2 with mFT augmented gRNA targeting NbAGDepositorInsertp35S:TRV2_NbAGsgRNA1_mFT:tNos
ExpressionPlantPromoter35SAvailable SinceJuly 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEE385
Plasmid#149289PurposeT-DNA encoding TRV2 with Truncated FT augmented gRNA targeting NbAGDepositorInsertp35S:TRV2_NbAGsgRNA2_TrunFT:tNos
ExpressionPlantPromoter35SAvailable SinceJuly 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pG20_GUS_Hyg
Plasmid#159705PurposepGREEN-based cloning construct for expression in plantaDepositorTypeEmpty backboneTagsGUSExpressionPlantAvailable SinceOct. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGREAT4
Plasmid#170892PurposeGoldenBraid Double Transcriptional Unit - pG10-90_E-Luc_NOSt+pNOS_Red-F_NOStDepositorInsertE-Luc
UseLuciferase and Synthetic BiologyExpressionPlantMutationc.375C>T silent mutation to remove BbsI site (…PromoterpG10-90Available SinceAug. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFGC5941-PacI-ath-STTM162
Plasmid#84088Purposetarget miRNA162 for destruction in Arabidopsis thalianaDepositorInsertSTTM162
ExpressionPlantPromoter2x35SAvailable SinceNov. 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGEL065
Plasmid#124896PurposeFor genome editing in plant by SpCas9 variant(SpCas9-NGv1)DepositorTypeEmpty backboneUseCRISPRExpressionPlantAvailable SinceJan. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFRETvr-2in1-NN
Plasmid#105126PurposeFRET/FLIM analysis in plants (stable or transient transformation)DepositorTypeEmpty backboneTagsmVenus/tagRFPExpressionPlantAvailable SinceFeb. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
lvl0 ACT2p
Plasmid#153374PurposePlant promoterDepositorInsertACT2 promoter
ExpressionPlantAvailable SinceOct. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
lvl0 RbcS2bt
Plasmid#153387PurposePlant terminatorDepositorInsertRbcS2b terminator
ExpressionPlantAvailable SinceOct. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
SUN2delSUN
Plasmid#130835PurposeExpression of plant LINC fluorescent fusion protein for cell biologyDepositorInsertSUN2
TagsmCherry-FLAG-HAExpressionPlantMutationDeletion of SUN domainPromoterCaMV 35SAvailable SinceAug. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
lvl0 TUB9t
Plasmid#153385PurposePlant terminatorDepositorInsertTUB9 terminator
ExpressionPlantAvailable SinceOct. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
lvl0 35St
Plasmid#153382PurposeCauliflower mosaic virus terminator for plant expressionDepositorInsert35S terminator
ExpressionPlantAvailable SinceOct. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEPOR2KN0038
Plasmid#117605PurposeLevel2 MoClo construct, containing level1 plant expression cassettes for LbCas12a(pEPOR1CB0013) and sgRNA_AtADH1 {AT1G77120}(pEPOR1CB0029)DepositorInsert[35S:LbCas12a(pEPOR1CB0013) ] +[AtU6-26:sgRNA]
ExpressionPlantAvailable SinceNov. 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMDC84-SEIPIN3-Ndelta
Plasmid#96981PurposeExpress N-terminal truncated Arabidopsis SEIPIN3 gene in plants.DepositorInsertSEIPIN3 (AT2G34380 Mustard Weed)
Tags6xHis and GFPExpressionPlantMutationA segment of 507 nucleotides are deleted from the…Promoter35SAvailable SinceJuly 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJG486
Plasmid#91195PurposeWDV replicon T-DNA for gene targeting in wheat scutella, H840A single nickase (nTaCas9_H840A+gUbi1+donor)DepositorInsertnTaCas9_H840A+gUbi1+donor
UseCRISPRExpressionPlantAvailable SinceJuly 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEGB3 alpha1 35s:PIF6:tNos (GB0170)
Plasmid#160606PurposeTU for the constitutive expression of a chimeric construct comprising a DNA binding protein fused to A. thaliana PIF6 (amino acids 1-100) and a nuclear localization sequence. Part of a red light-controlled synthetic genetic switchDepositorInsertPIF6
UseSynthetic BiologyExpressionPlantMutationBsaI and BsmBI sites removedPromoterP35SAvailable SinceMarch 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
INCTbiosyn-p35S-GFP(rc)4
Plasmid#127522PurposePlasmid has a CaMV 35S promoter in a forward sequence orientation and an inverted EGFP coding sequence (reverse complement) flanked by attB and attP integrase 4 attachment sites.DepositorInsertEGFP reverse complement coding sequence flanked by attB/attP Integrase 4 attachment sites.
ExpressionPlantAvailable SinceMay 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDIRECT_26K
Plasmid#91152PurposeDirect Cloning, Type: T-DNA, Engineering Reagent: ZmUbi:TALEN, Plant Selection: PvUbi2:barDepositorInsertEngineering Reagent: ZmUbi:TALEN
UseTALENExpressionPlantAvailable SinceJan. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDIRECT_23J
Plasmid#91142PurposeDirect Cloning, Type: T-DNA, Engineering Reagent: 35S:TALEN, Plant Selection: 2x35S:barDepositorInsertEngineering Reagent: 35S:TALEN
UseTALENExpressionPlantAvailable SinceJuly 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDIRECT_22J
Plasmid#91137PurposeDirect Cloning, Type: T-DNA, Engineering Reagent: 35S:TALEN, Plant Selection: 2x35S:npt IIDepositorInsertEngineering Reagent: 35S:TALEN
UseTALENExpressionPlantAvailable SinceJuly 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB223
Plasmid#68614PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneTagsTriple repeats of Citrine (3xCitrine)ExpressionPlantAvailable SinceJune 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB226
Plasmid#68617PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneTagsTagRFPExpressionPlantAvailable SinceNov. 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEGB 35s:dCas:VP64:tNos (GB1189)
Plasmid#75403PurposeTranscriptional unit of (human codon optimized) inactivated Cas9 fused to the VP64 Transcriptional ActivatorDepositorInsertdCas:VP64
UseCRISPR and Synthetic BiologyExpressionPlantPromoter35SAvailable SinceAug. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFLPe-AntiDsRed
Plasmid#139676PurposeCassette for testing FLPe recombinase activity using a fluorescent reporterDepositorInsertAntiDsRed FLPe
ExpressionPlantPromoterMaize UbiquitinAvailable SinceMay 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB209
Plasmid#68600PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneTags3xFLAGExpressionPlantAvailable SinceJune 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTRANS_130
Plasmid#91204PurposeTransformation, Type: non-T-DNA, Plant Selection: 2x35S:bar, Viral Replication: noneDepositorTypeEmpty backboneExpressionPlantAvailable SinceJuly 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pYPQ134-STU-Fn
Plasmid#138104PurposeGolden Gate entry vector for 4th crRNA cloning with FnCas12a crRNA scaffold flanked by HH and HDV ribozymesDepositorTypeEmpty backboneUseCRISPRExpressionPlantAvailable SinceMarch 31, 2021AvailabilityAcademic Institutions and Nonprofits only -
pYPQ131-STU-Fn
Plasmid#138095PurposeGolden Gate entry vector for 1st crRNA cloning with FnCas12a crRNA scaffold flanked by HH and HDV ribozymesDepositorTypeEmpty backboneUseCRISPRExpressionPlantAvailable SinceMarch 31, 2021AvailabilityAcademic Institutions and Nonprofits only