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pTRANS_101

Plasmid

#91199

Purpose

Transformation, Type: non-T-DNA, Plant Selection: none, Viral Replication: BeYDV

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Type

Empty backbone

Expression

Plant

Available Since

July 7, 2017

Availability

Academic Institutions and Nonprofits only

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pCMB-TPr

Plasmid

#61190

Purpose

Fluorescent reporter for tonoplast AtTIP1-1-mCherry expressed under MtBCP1 promoter

Depositor

Maria Harrison

Article

Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

Insert

AtTIP1-1

Tags

mCherry

Expression

Plant

Promoter

MtBCP1

Available Since

April 23, 2015

Availability

Academic Institutions and Nonprofits only

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pGEL030

Plasmid

#137899

Purpose

STU2.0 SpyCas9 Csy4 system for plant genome editing

Depositor

Yong Zhang

Article

Tang et al Plant Biotechnol J. 2019 Jul;17(7):1431-

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available Since

Aug. 31, 2020

Availability

Academic Institutions and Nonprofits only

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pNK869

Plasmid

#219737

Purpose

MoClo-compatible Level 0 - promoter + 5U vector carrying promoter AtPD7

Depositor

Alexander Mishin

Article

Shakhova et al Int J Mol Sci. 2022 Dec 6;23(23):15441.

Insert

promoter of serine carboxypeptidase-like gene AtSCPL30, AtPD7

Use

Synthetic Biology

Expression

Plant

Promoter

AtPD7

Available Since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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pAtCas9_3

Plasmid

#91158

Purpose

Cas9 only expression, Cas9 Type: AtCas9, Plant Selection: 2x35S:bar

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

AtCas9

Use

CRISPR

Expression

Plant

Available Since

July 10, 2017

Availability

Academic Institutions and Nonprofits only

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pFRETgc-2in1-NN

Plasmid

#105124

Purpose

FRET/FLIM analysis in plants (stable or transient transformation)

Depositor

Christopher Grefen

Article

Hecker et al Plant Physiol. 2015 Jul;168(3):776-87. d

Type

Empty backbone

Tags

mEGFP/mCherry

Expression

Plant

Available Since

Feb. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pMpGWB228

Plasmid

#68619

Purpose

Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

Depositor

Takayuki Kohchi

Article

Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

Type

Empty backbone

Tags

TagRFP

Expression

Plant

Promoter

Cauliflower mosaic virus 35S promoter

Available Since

June 24, 2016

Availability

Academic Institutions and Nonprofits only

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pYPQ141D-gRNA2.1

Plasmid

#167161

Purpose

Express single gRNA with gRNA2.1 scaffold (with four MS2 binding sites) under OsU3 promoter

Depositor

Yiping Qi

Article

Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:

Type

Empty backbone

Use

CRISPR

Expression

Plant

Promoter

OsU3

Available Since

June 25, 2021

Availability

Academic Institutions and Nonprofits only

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P527

Plasmid

#139741

Purpose

Individual component of Caffeic Acid biosynthesis pathway, RcTAL

Depositor

Daniel Voytas

Article

Khakhar et al Elife. 2020 Mar 25;9. pii: 52786. doi: 1

Insert

TAL

Expression

Plant

Promoter

35S

Available Since

May 29, 2020

Availability

Academic Institutions and Nonprofits only

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pFRETgc-2in1-CN

Plasmid

#105118

Purpose

FRET/FLIM analysis in plants (stable or transient transformation)

Depositor

Christopher Grefen

Article

Hecker et al Plant Physiol. 2015 Jul;168(3):776-87. d

Type

Empty backbone

Tags

mCherry and mEGFP

Expression

Plant

Available Since

Feb. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pGem-nHyPer:sAPX

Plasmid

#84735

Purpose

Simultaneous expresion of stromal HyPer2 and stromal Ascorbate peroxidase (APX) in plant cells

Depositor

Philip Mullineaux

Article

Exposito-Rodriguez et al Nat Commun. 2017 Jun 29;8(1):49. doi: 10

Inserts

sAPX (SAPX Mustard Weed)
nHyper (HyPer2 targeted to nucleus)

Tags

nuclear localisation signals from the coding sequ…

Expression

Plant

Promoter

FMV/35S

Available Since

July 6, 2017

Availability

Academic Institutions and Nonprofits only

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pDGE463

Plasmid

#153234

Purpose

Recipient plant transformation vector containing selection markers and a Cas9 expression cassette, but lacking guide RNAs

Depositor

Sylvestre Marillonnet

Article

Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available Since

Sept. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pSB137 - pL2_pSB90_pNOS::rLUC-I::tNOS_tOCS::fLUC-I::pSTR1

Plasmid

#123194

Purpose

binary plant vector for transient dual luciferase (rLUC & fLUC with introns) expression

Depositor

Erin Cram, Carolyn Lee-Parsons

Article

Mortensen et al Front Plant Sci. 2019 Jun 11;10:755. doi

Insert

pNOS::rLUC-I::tNOS tOCS::fLUC-I::pSTR1

Use

Luciferase

Expression

Plant

Available Since

May 29, 2019

Availability

Academic Institutions and Nonprofits only

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P364

Plasmid

#139721

Purpose

Module plasmid, individual component of Fungal Bioluminescent Pathway, CPH

Depositor

Daniel Voytas

Article

Khakhar et al Elife. 2020 Mar 25;9. pii: 52786. doi: 1

Insert

pMOD_A'_pSlH4:NnCPH:tSlH4

Use

Luciferase

Expression

Plant

Promoter

SlH4

Available Since

May 29, 2020

Availability

Academic Institutions and Nonprofits only

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pYI001

Plasmid

#170833

Purpose

(GUSPlus::tOCS in pFP100)*

Depositor

Christian Fankhauser

Article

Ince et al Nat Commun. 2022 Oct 10;13(1):5659. doi:

Insert

GUSPlus::tOCS

Expression

Plant

Available Since

Oct. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pJG85

Plasmid

#89281

Purpose

Entry vector containing the TaU6 promoter and an Esp3I Golden Gate cloning site for cloning of the sgRNA, all between attL5 and attL2 sites

Depositor

Daniel Voytas

Article

Gil-Humanes et al Plant J. 2016 Dec 10. doi: 10.1111/tpj.1

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available Since

May 1, 2017

Availability

Academic Institutions and Nonprofits only

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pMpGWB122

Plasmid

#68576

Purpose

Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

Depositor

Takayuki Kohchi

Article

Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

Type

Empty backbone

Tags

Modified EAR motif plant-specific repression doma…

Expression

Plant

Promoter

Cauliflower mosaic virus 35S promoter

Available Since

July 13, 2016

Availability

Academic Institutions and Nonprofits only

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pROF346

Plasmid

#155331

Purpose

Binary vector for expression of PULSE system (under the control of AtUbi10 promoter) controlling Venus-H2B. It constitutively expresses Cerulean-NLS and a plant selection cassette.

Depositor

Matias Zurbriggen

Article

Ochoa-Fernandez et al Nat Methods. 2020 Jul;17(7):717-725. doi

Insert

RB_KanR_Tnos-NLS-PIF6-E-PUbi10_Tnos-nCerulean-PUbi10_Tnos-NLS-VP16-PhyB-PUbi10_Tnos-EL222-NLS-SRDX-PUbi10_T35S-nVenus-POpto_LB

Use

Synthetic Biology

Expression

Plant

Available Since

Oct. 26, 2023

Availability

Academic Institutions and Nonprofits only

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pCaRNA3-eIFiso4E120(+)

Plasmid

#86092

Purpose

This vector is used to specifically silence the eIF(iso)4E gene in peach via VIGS.

Depositor

Aiming Wang

Article

Cui et al Plant Biotechnol J. 2016 Aug 27. doi: 10

Insert

eIFiso4E

Expression

Plant

Available Since

Jan. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pGEL033

Plasmid

#137902

Purpose

STU2.0 rAPOBEC1-SpyCas9(D10A) for plant genome base editing

Depositor

Yong Zhang

Article

Tang et al Plant Biotechnol J. 2019 Jul;17(7):1431-

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available Since

Feb. 25, 2020

Availability

Academic Institutions and Nonprofits only

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