4,240 results

1. pYPQ-dpcoCas9-EV2.1

   Plasmid

   #158411

   Purpose

   CRISPR-dpcoCas9 fused with EDLL activator for transcriptional activation

   Depositor

   Yiping Qi

   Article

   Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:

   Insert

   dpcoCas9-EDLL-T2A-MS2-VPR(VP64-p65-Rta)-NOS-ter
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   June 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pFH22

   Plasmid

   #125808

   Purpose

   Level0 xCas9 3.7

   Depositor

   Vladimir Nekrasov

   Article

   Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

   Insert

   xCas9 3.7
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Aug. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pFH55

   Plasmid

   #125814

   Purpose

   Level0 nSpCas9-PmCDA, Arabidopsis codon optimized

   Depositor

   Vladimir Nekrasov

   Article

   Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

   Insert

   nSpCas9-PmCDA, Arabidopsis codon optimized
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Aug. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMY200

   Plasmid

   #69946

   Purpose

   Agrobacterium-based transformation vector

   Depositor

   Mark Farman

   Type

   Empty backbone

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Oct. 13, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pMpGWB326

   Plasmid

   #68654

   Purpose

   Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

   Depositor

   Takayuki Kohchi

   Article

   Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

   Type

   Empty backbone

   Use

   Tags

   TagRFP

   Expression

   Plant

   Mutation

   Promoter

   No Promoter

   Available since

   June 24, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pFH33

   Plasmid

   #125885

   Purpose

   Level0 Wheat TaU3 Pol III promoter with transcription Start-A

   Depositor

   Vladimir Nekrasov

   Article

   Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

   Insert

   Level0 Wheat TaU3 Pol III promoter with transcription Start-A
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Aug. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pC1300_pUB10_pco-vCASphi_E9t_V2_CmYLCVp_35sT_ribozyme_AtPDS3_gRNA10

   Plasmid

   #197966

   Purpose

   T-DNA binary vector to express pco-vCasphi driven by UBQ10 gene promoter, and the AtPDS3 guide RNA 10 driven by CmYLCVp, flanked by ribozymes.

   Depositor

   Steven Jacobsen

   Article

   Li et al Proc Natl Acad Sci U S A. 2023 Jan 24;12

   Inserts

   pco-vCasphi-2
   AtPDS3 gRNA10
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   CmYLCVp and pUBQ10

   Available since

   March 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pYPQ289 (ErCas12a)

   Plasmid

   #138126

   Purpose

   Gateway entry plasmid (attL1 & attR5) expressing rice codon optimized ErCas12a without promoter

   Depositor

   Yiping Qi

   Article

   Zhang et al Nat Commun. 2021 Mar 29;12(1):1944. doi:

   Insert

   ErCas12a
   

   Use

   CRISPR; Gateway compatible cas12a entry clone

   Tags

   NLS

   Expression

   Plant

   Mutation

   Promoter

   Available since

   March 31, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. INCTbiosyn-pAct-AtINTBxb1

   Plasmid

   #127536

   Purpose

   Plasmid encodes A. thaliana codon optimized Integrase Bxb1.

   Depositor

   Martin Bonamino

   Article

   Gomide et al Commun Biol. 2020 May 22;3(1):255. doi:

   Insert

   Integrase Bxb1 coding sequence codon optimized for A. thaliana expression.
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   May 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pYPQ141-ZmUbi-RZ-Aac.4

    Plasmid

    #158406

    Purpose

    Express single gRNA with Aac.4 scaffold (with four MS2 binding sites) under ZmUbi promoter

    Depositor

    Yiping Qi

    Article

    Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Maize ubiquitin 1

    Available since

    June 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAK-EL-02

    Plasmid

    #125761

    Purpose

    Endlinker for tRNA-gRNA system when using only two guides

    Depositor

    Vladimir Nekrasov

    Article

    Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

    Insert

    Endlinker
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Aug. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. A3A-PBE-ΔUGI

    Plasmid

    #119770

    Purpose

    Conversion of C to T in wheat plants or monocotyledons protoplasts without uracil glycosylase inhibitor (UGI)

    Depositor

    Caixia Gao

    Article

    Zong et al Nat Biotechnol. 2018 Oct 1. pii: nbt.426

    Insert

    APOBEC3A-nCas9-ΔUGI-NLS
    

    Use

    Tags

    NLS

    Expression

    Plant

    Mutation

    nCas9 has a mutation D10A in wild type SpCas9

    Promoter

    maize Ubiquitin-1

    Available since

    Dec. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pFGC5941-PacI-ath-STTM159

    Plasmid

    #84084

    Purpose

    target miRNA159 for destruction in Arabidopsis thaliana

    Depositor

    Guiliang Tang

    Article

    Tang et al Methods. 2012 Oct;58(2):118-25. doi: 10.

    Insert

    STTM159
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    2x35S

    Available since

    March 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. lvl0 MASp

    Plasmid

    #153364

    Purpose

    Agrobacterium promoter for plant expression

    Depositor

    Naomi Nakayama

    Article

    Andreou et al bioRxiv, March 31, 2021

    Insert

    MAS promoter
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Nov. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pYPQ-dAaCas12b-Act3.0

    Plasmid

    #158413

    Purpose

    CRISPR-Act3.0 system containing dAaCas12b-VP64 fusion protein, T2A linked MS2-SunTag fusion protein, and ScFv-sfGFP-2xTAD activator.

    Depositor

    Yiping Qi

    Article

    Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:

    Insert

    dAaCas12b-VP64-T2A-MS2-SunTag-rbcS-E9t-ter-ZmUbi-scFv-sfGFP-2xTAD-GB1-NOS-ter
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    July 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pICSL1P2_crU626

    Plasmid

    #191777

    Purpose

    Cas12a guide expression in dicots

    Depositor

    Wendy Harwood

    Article

    Lawrenson et al Methods Mol Biol. 2023;2653:253-271. doi

    Insert

    LbCas12a guide scaffold with AtU626 promoter/terminator & flanking ribozymes
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Nov. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pICSL1P7_crU626

    Plasmid

    #191782

    Purpose

    Cas12a guide expression in dicots

    Depositor

    Wendy Harwood

    Article

    Lawrenson et al Methods Mol Biol. 2023;2653:253-271. doi

    Insert

    LbCas12a guide scaffold with AtU626 promoter/terminator & flanking ribozymes
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Nov. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. PDF1::mCitrine-MBD

    Plasmid

    #115222

    Purpose

    Microtubule visualization

    Depositor

    Jan Traas

    Article

    Armezzani et al Development. 2018 Jun 4;145(11). pii: de

    Insert

    Microtubule binding domain
    

    Use

    Tags

    mCitrine

    Expression

    Plant

    Mutation

    Promoter

    Available since

    April 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pDGB3 alpha2 P35S:MS2:EDLL:Tnos (GB1738)

    Plasmid

    #160622

    Purpose

    TU for the constitutive expression of the MS2 coat protein fused on Ct to the activation domain EDLL

    Depositor

    Diego Orzaez

    Article

    Selma et al Plant Biotechnol J. 2019 Sep;17(9):1703-

    Insert

    P35s_MS2:EDLL_Tnos
    

    Use

    Tags

    Expression

    Plant

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    P35S

    Available since

    March 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pORFE0005

    Plasmid

    #112073

    Purpose

    AtDeadCAS9 D10A H840A without stop codon module CDS1ns

    Depositor

    Christian Chevalier

    Insert

    AtDeadCAS9 D10A H840A without stop codon
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Plant codon optimized dCas9 D10A S. pyogenes

    Promoter

    Available since

    Aug. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge