4,305 results
-
Plasmid#158521PurposeIn planta gene expression of Golden gate compatible A. thaliana ZAR1 L17E-eGFP. Km plant selection marker.DepositorInsertZAR1-eGFP (ZAR1 Mustard Weed)
UseTagseGFPExpressionPlantMutationL17EPromoterAvailable sinceAug. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pZA29
Plasmid#158507PurposeIn planta gene expression of Golden gate compatible N. benthamiana ZAR1-4xMyc. Km plant selection marker.DepositorInsertZAR1-4xMyc
UseTags4xMycExpressionPlantMutationPromoterAvailable sinceAug. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pZA38
Plasmid#158516PurposeIn planta gene expression of Golden gate compatible N. benthamiana ZAR1 D481V-6xHA. Km plant selection marker.DepositorInsertZAR1-6xHA
UseTags6xHAExpressionPlantMutationD481VPromoterAvailable sinceAug. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pZA42
Plasmid#158520PurposeIn planta gene expression of Golden gate compatible A. thaliana ZAR1 D498V-eGFP. Km plant selection marker.DepositorInsertZAR1-eGFP (ZAR1 Mustard Weed)
UseTagseGFPExpressionPlantMutationD489VPromoterAvailable sinceAug. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pZA34
Plasmid#158512PurposeIn planta gene expression of Golden gate compatible A. thaliana ZAR1 D498V-6xHA. Km plant selection marker.DepositorInsertZAR1-6xHA (ZAR1 Mustard Weed)
UseTags6xHAExpressionPlantMutationD489VPromoterAvailable sinceAug. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pZA28
Plasmid#158506PurposeIn planta gene expression of Golden gate compatible A. thaliana ZAR1 D498V/L17E-4xMyc. Km plant selection marker.DepositorInsertZAR1-4xMyc (ZAR1 Mustard Weed)
UseTags4xMycExpressionPlantMutationL17E, D489VPromoterAvailable sinceAug. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pZA23
Plasmid#158501PurposeIn planta gene expression of Golden gate compatible N. benthamiana ZAR1 L17E. Km plant selection marker.DepositorInsertZAR1
UseTagsExpressionPlantMutationL17EPromoterAvailable sinceAug. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pZA25
Plasmid#158503PurposeIn planta gene expression of Golden gate compatible A. thaliana ZAR1-4xMyc. Km plant selection marker.DepositorInsertZAR1-4xMyc (ZAR1 Mustard Weed)
UseTags4xMycExpressionPlantMutationPromoterAvailable sinceAug. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pZA46
Plasmid#158524PurposeIn planta gene expression of Golden gate compatible N. benthamiana ZAR1 D481V-eGFP. Km plant selection marker.DepositorInsertZAR1-eGFP
UseTagseGFPExpressionPlantMutationD481VPromoterAvailable sinceAug. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pZA47
Plasmid#158525PurposeIn planta gene expression of Golden gate compatible N. benthamiana ZAR1 L17E-eGFP. Km plant selection marker.DepositorInsertZAR1-eGFP
UseTagseGFPExpressionPlantMutationL17EPromoterAvailable sinceAug. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pZA48
Plasmid#158526PurposeIn planta gene expression of Golden gate compatible N. benthamiana ZAR1 D481V/L17E-eGFP. Km plant selection marker.DepositorInsertZAR1-eGFP
UseTagseGFPExpressionPlantMutationL17E, D481VPromoterAvailable sinceAug. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAGM41991
Plasmid#153219PurposeLevel M binary vector for plant transformation; for cloning Cas9 and guide RNAs; single copy in AgrobacteriumDepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceAug. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pYPQ265H
Plasmid#149554PurposeGateway entry clone for CRISPR-iSpyMacCas9 hAID C-T base editing at NAAR PAMsDepositorInserthAID-ziSpyMacCas9(D10A)-UGI
UseCRISPRTagsExpressionPlantMutationD10A, R221K, N394K, Protospacer interacting domai…PromoterAvailable sinceAug. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
UBQ10:sXVE:S11-GFP1–9
Plasmid#125665PurposeBinary vector for the inducible expression of S11-GFP1–9 fragment that can be used to detect a protein tagged with GFP10DepositorInsertS11-GFP1–9
UseSynthetic BiologyTagsExpressionPlantMutationPromoterUBQ10:sXVE:Available sinceAug. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
UBQ10:sXVE:ER-S11-GFP1–9
Plasmid#125666PurposeBinary vector for the inducible expression of ER-targeted GFP1–9 fragment that can be used to detect a protein tagged with GFP10DepositorInsertER-S11-GFP1–9
UseSynthetic BiologyTagsExpressionPlantMutationPromoterUBQ10:sXVE:Available sinceAug. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDGE502
Plasmid#153275PurposeM8E shuttle vector containing the Solanum lycopersicum U3 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.DepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pYPQ266B
Plasmid#149555PurposeGateway entry clone for CRISPR-iSpyMacCas9 PmCDA1 C-T base editing at NAAR PAMsDepositorInsertziSpyMacCas9(D10A)-PmCDA1-UGI
UseCRISPRTagsExpressionPlantMutationD10A, R221K, N394K, Protospacer interacting domai…PromoterAvailable sinceJuly 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
Six5 pGWB402
Plasmid#118013Purposeexpresses Six5 (without signal peptide). Used in combination with Avr2-GFP pZK538(#118011) for visualization of cell-to-cell movement of Avr2DepositorArticleInsertdspSix5
UseTagsExpressionBacterial and PlantMutationPromoter35SAvailable sinceJune 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
P302-FBP_1
Plasmid#139692PurposeT-DNA encoding Fungal Bioluminescent Pathway Components, complete pathway, without recycling gene CPHDepositorInsertp35S_Short_TMV 5':AsNpga:tAtug7, AtUbi10:NnH3H:AtuOCS, pCmYLCV:NnHisps:tHsp, pAtuMas:NnLuz:trbcSE9
UseLuciferaseTagsExpressionPlantMutationPromotervariousAvailable sinceMay 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
P313-FBP_8
Plasmid#139699PurposeT-DNA encoding Fungal Bioluminescent Pathway Components, control pathway, missing H3HDepositorInsertpLHB1B1:NnCPH:tSlH4, p35S_Short_TMV 5':AsNpga:tAtug7, pCmYLCV:NnH3H:AtuOcs, pCmYLCV:NnHisps:tHsp, pAtuMas:Nn Luz:trbcSE9
UseLuciferaseTagsExpressionPlantMutationPromotervariousAvailable sinceMay 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pR-AntiDsRed
Plasmid#139678PurposeCassette for testing R recombinase activity using a fluorescent reporterDepositorInsertAntiDsRed RS
UseTagsExpressionPlantMutationPromoterMaize UbiquitinAvailable sinceMay 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pIntegrase-AntiDsRed
Plasmid#139677PurposeCassette for testing phiC31 Integrase activity using a DsRed2 fluorescent reporterDepositorInsertAntiDsRed Integrase
UseTagsExpressionPlantMutationPromoterMaize UbiquitinAvailable sinceMay 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pExcisionase-AntiDsRed
Plasmid#139675PurposeCassette for testing phiC31 Excisionase activity using a fluorescent reporterDepositorInsertAntiDsRed phiC31 Excisionase
UseTagsExpressionPlantMutationPromoterMaize UbiquitinAvailable sinceMay 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEarleyGate100-pSpoA-APVNTT-DsRed-Express2-FKBP(LV)(C22V)
Plasmid#140170PurposeExpresses a regulatable secretory cargo for Arabidopsis thalianaDepositorInsertpSpoA-APVNTT-DsRed-Express2-FKBP(LV)(C22V)
UseTagsExpressionPlantMutationPromoterCaMV 35SAvailable sinceMay 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEarleyGate100-pSpoA-DsRed-Express2-FKBP(LV)(C22V)
Plasmid#140169PurposeExpresses a regulatable secretory cargo (without an ER export signal) for Arabidopsis thalianaDepositorInsertpSpoA-DsRed-Express2-FKBP(LV)(C22V)
UseTagsExpressionPlantMutationPromoterCaMV 35SAvailable sinceMay 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
INCTbiosyn-p35S-GFP(rc)5
Plasmid#127523PurposePlasmid has a CaMV 35S promoter in a forward sequence orientation and an inverted EGFP coding sequence (reverse complement) flanked by attB and attP integrase 5 attachment sites.DepositorInsertEGFP reverse complement coding sequence flanked by attB/attP Integrase 5 attachment sites.
UseTagsExpressionPlantMutationPromoterAvailable sinceMay 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
INCTbiosyn-p35S-GFP(rc)7
Plasmid#127524PurposePlasmid has a CaMV 35S promoter in a forward sequence orientation and an inverted EGFP coding sequence (reverse complement) flanked by attB and attP integrase 7 attachment sites.DepositorInsertEGFP reverse complement coding sequence flanked by attB/attP Integrase 7 attachment sites.
UseTagsExpressionPlantMutationPromoterAvailable sinceMay 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
INCTbiosyn-p35S-GFP(rc)9
Plasmid#127525PurposePlasmid has a CaMV 35S promoter in a forward sequence orientation and an inverted EGFP coding sequence (reverse complement) flanked by attB and attP integrase 9 attachment sites.DepositorInsertEGFP reverse complement coding sequence flanked by attB/attP Integrase 9 attachment sites.
UseTagsExpressionPlantMutationPromoterAvailable sinceMay 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
INCTbiosyn-p35S-GFP(rc)2
Plasmid#127521PurposePlasmid has a CaMV 35S promoter in a forward sequence orientation and an inverted EGFP coding sequence (reverse complement) flanked by attB and attP integrase 2 attachment sites.DepositorInsertEGFP reverse complement coding sequence flanked by attB/attP Integrase 2 attachment sites.
UseTagsExpressionPlantMutationPromoterAvailable sinceMay 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCk4
Plasmid#136698PurposeAcceptor plasmid for odd level position 4, lacZ blue-white screening. Part of the OpenPlant toolkit.DepositorInsertlacZ
UseSynthetic BiologyTagsExpressionPlantMutationBsaI/ SapI domesticatedPromoterAvailable sinceMarch 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pYPQ290-D977A
Plasmid#129674PurposeGateway entry clone for CRISPR-Cas12b systemsDepositorInsertAacCas12b
UseCRISPR; Gateway compatible aaccas12b entry cloneTagsExpressionPlantMutationD977A; AacCas12b is rice codon optimizedPromoterAvailable sinceMarch 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pYPQ290-E848A
Plasmid#129675PurposeGateway entry clone for CRISPR-Cas12b systemsDepositorInsertAacCas12b
UseCRISPR; Gateway compatible aaccas12b entry cloneTagsE848A; AacCas12b is rice codon optimizedExpressionPlantMutationPromoterAvailable sinceMarch 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pYPQ291-D573A
Plasmid#129676PurposeGateway entry clone for CRISPR-Cas12b systemsDepositorInsertBthCas12b
UseCRISPR; Gateway compatible bthcas12b entry cloneTagsExpressionPlantMutationD573A; BthCas12b is rice codon optimizedPromoterAvailable sinceMarch 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pYPQ291-D951A
Plasmid#129677PurposeGateway entry clone for CRISPR-Cas12b systemsDepositorInsertBthCas12b
UseCRISPR; Gateway compatible bthcas12b entry cloneTagsExpressionPlantMutationD951A; BthCas12b is rice codon optimizedPromoterAvailable sinceMarch 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pYPQ291-E827A
Plasmid#129678PurposeGateway entry clone for CRISPR-Cas12b systemsDepositorInsertBthCas12b
UseCRISPR; Gateway compatible bthcas12b entry cloneTagsExpressionPlantMutationE827A; BthCas12b is rice codon optimizedPromoterAvailable sinceMarch 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pYPQ292-D977A
Plasmid#129680PurposeGateway entry clone for CRISPR-Cas12b systemsDepositorInsertAaCas12b
UseCRISPR; Gateway compatible aacas12b entry cloneTagsExpressionPlantMutationD977A; AaCas12b is rice codon optimizedPromoterAvailable sinceMarch 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pYPQ292-E848A
Plasmid#129681PurposeGateway entry clone for CRISPR-Cas12b systemsDepositorInsertAaCas12b
UseCRISPR; Gateway compatible aacas12b entry cloneTagsExpressionPlantMutationE848A; AaCas12b is rice codon optimizedPromoterAvailable sinceMarch 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pYPQ290-D570A-SRDX
Plasmid#129682PurposeGateway entry clone for CRISPR-Cas12b systemsDepositorInsertAacCas12b-SRDX
UseCRISPR; Gateway compatible aaccas12b entry cloneTagsExpressionPlantMutationD570A; AacCas12b is rice codon optimizedPromoterAvailable sinceMarch 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pYPQ291-D573A-SRDX
Plasmid#129683PurposeGateway entry clone for CRISPR-Cas12b systemsDepositorInsertBthCas12b-SRDX
UseCRISPR; Gateway compatible bthcas12b entry cloneTagsExpressionPlantMutationD573A; BthCas12b is rice codon optimizedPromoterAvailable sinceMarch 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pYPQ141-ZmUbi-RZ-Bth
Plasmid#129686Purpose(Empty backbone) Gateway entry clone with sgRNA under ZmUbi promoterDepositorInsertEmpty Backbone
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceMarch 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGEL077
Plasmid#137886PurposeiSTU-CRISPR/Cas9 with StIV2 intron, NU structure backboneDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceFeb. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGEL050
Plasmid#137904PurposeDual-mini35S driven Cas9-Csy4 systemDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceFeb. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGEL051
Plasmid#137905PurposeDual-mini35S-enhancer driven Cas9-Csy4 systemDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceFeb. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGEL079
Plasmid#137888PurposeiSTU-CRISPR/Cas9 with StIV2 intron, RZ structure backboneDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceFeb. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGEL078
Plasmid#137887PurposeiSTU-CRISPR/Cas9 with StIV2 intron, tRNA structure backboneDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceFeb. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pYPQ141-ZmUbi-RZ-Aa1.2
Plasmid#136373PurposeGateway entry clone for AaCas12b sgRNA expression under ZmUbi promoter with ribozyme processing; sgRNA scaffold 1.2 is usedDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceFeb. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pYPQ141-ZmUbi-RZ-Aa3.8.3
Plasmid#136374PurposeGateway entry clone for AaCas12b sgRNA expression under ZmUbi promoter with ribozyme processing; sgRNA scaffold 3.8 with MS2 at the third stem loop is usedDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceFeb. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pYPQ141-ZmUbi-RZ-Aac.3
Plasmid#136377PurposeGateway entry clone for AacCas12b sgRNA expression under ZmUbi promoter with ribozyme processing; sgRNA scaffold with MS2 at the third stem loop is usedDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceFeb. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pYPQ141-ZmUbi-RZ-Bh
Plasmid#136378PurposeGateway entry clone for BhCas12b sgRNA expression under ZmUbi promoter with ribozyme processingDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceFeb. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pYPQ141-ZmUbi-RZ-Aa1.2.3
Plasmid#136372PurposeGateway entry clone for AaCas12b sgRNA expression under ZmUbi promoter with ribozyme processing; sgRNA scaffold 1.2 with MS2 at the third stem loop is usedDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceFeb. 18, 2020AvailabilityAcademic Institutions and Nonprofits only