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pUBQ10:AtFLS2

Plasmid

#202186

Purpose

Express Arabidopsis thaliana FLS2 gene under UBQ10 promoter

Depositor

Gitta Coaker

Article

Trinh et al Plant Physiol. 2023 May 5:kiad263. doi:

Insert

Flagellin sensing 2 (FLS2 Mustard Weed)

Tags

HA

Expression

Plant

Promoter

AtUBQ10

Available Since

Sept. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pCO486

Plasmid

#139729

Purpose

Module plasmid, individual component of Fungal Bioluminescent Pathway, Npga

Depositor

Daniel Voytas

Article

Khakhar et al Elife. 2020 Mar 25;9. pii: 52786. doi: 1

Insert

pMOD_A_35S Short_TMV 5':AsNpga:tAtug7

Use

Luciferase

Expression

Plant

Promoter

35S

Available Since

May 29, 2020

Availability

Academic Institutions and Nonprofits only

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pUBN-paGFP-Dest

Plasmid

#105108

Purpose

fluorescent tagging in stably transformed plants

Depositor

Christopher Grefen

Article

Blatt et al Methods Mol Biol. 2014;1062:487-507. doi

Type

Empty backbone

Tags

paGFP

Expression

Plant

Available Since

Feb. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pFGC5941DR5-PacI-ath-STTM390

Plasmid

#84117

Purpose

target miRNA390 for destruction in Arabidopsis thaliana

Depositor

Guiliang Tang

Article

Tang et al Methods. 2012 Oct;58(2):118-25. doi: 10.

Insert

STTM390

Expression

Plant

Promoter

2x35S

Available Since

March 21, 2017

Availability

Academic Institutions and Nonprofits only

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pAtCas9_1

Plasmid

#91156

Purpose

Cas9 only expression, Cas9 Type: AtCas9, Plant Selection: 2x35S:hpt II

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

AtCas9

Use

CRISPR

Expression

Plant

Available Since

July 10, 2017

Availability

Academic Institutions and Nonprofits only

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pMRMTK-clo10

Plasmid

#203961

Purpose

Cloning plasmid containing the erythromycin resistance gene insert

Depositor

Nathan Crook

Article

van Schaik et al ACS Synth Biol. 2023 Oct 20;12(10):3030-

Insert

Erythromycin Resistance Gene

Expression

Plant

Promoter

N/A

Available Since

Dec. 5, 2023

Availability

Academic Institutions and Nonprofits only

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35S::Cel1SP-GeNL/pCAMBIA1301

Plasmid

#190069

Purpose

Expressing bioluminescent pH indicator-GeNL- in Arabidopsis thaliana apoplast

Depositor

Takeharu Nagai

Article

Tran et al Plant Cell Environ. 2022 Jul 21. doi: 10

Insert

Cel1 signal peptide-Green-enhanced Nano-lantern

Expression

Plant

Promoter

CaMV 35S

Available Since

Feb. 9, 2023

Availability

Academic Institutions and Nonprofits only

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p16 GPS1

Plasmid

#101083

Purpose

GA biosensor expression plasmid

Depositor

Wolf Frommer, Alexander Jones

Article

Rizza et al Nat Plants. 2017 Oct 2. doi: 10.1038/s41

Insert

GPS1

Tags

cMyc

Expression

Plant

Promoter

p16 (AT3G60245)

Available Since

Oct. 3, 2017

Availability

Academic Institutions and Nonprofits only

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pMDC32B-OsMIR390-B/c

Plasmid

#61467

Purpose

Plant expression vector (2x35S) for direct cloning of artificial microRNAs into rice MIR390 precursor. To use for expressing amiRNAs in monocots.

Depositor

James Carrington

Article

Carbonell et al Plant J. 2015 Mar 24. doi: 10.1111/tpj.1

Insert

OsMIR390-B/c

Expression

Plant

Mutation

O. sativa MIR390 precursor sequence including a c…

Promoter

2x35S

Available Since

March 2, 2015

Availability

Academic Institutions and Nonprofits only

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pEGB Tnos:NptII:Pnos (GB0226)

Plasmid

#68212

Purpose

Transcriptional unit for kanamycin resistance gene plant expression

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

NptII

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI and BsmBI sites removed

Promoter

Pnos

Available Since

Oct. 14, 2015

Availability

Academic Institutions and Nonprofits only

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pNapin-eGFP

Plasmid

#127797

Purpose

Napin Promoter driven eGFP expression

Depositor

Scott Poethig

Article

Wu et al Genetics. 2015 May;200(1):35-45. doi: 10

Type

Empty backbone

Expression

Plant

Available Since

July 11, 2019

Availability

Academic Institutions and Nonprofits only

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LLP726_L2_pV01_0I_TMV-tGFP_TCTP-fLUC

Plasmid

#192406

Purpose

To be a control plasmid that has no Rluc repression (replaced by turboGFP) and should reflect true off state of the circuit.

Depositor

Ryan Lister

Article

Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

Insert

Act2::Turbo GFP

Use

Synthetic Biology

Tags

N7

Expression

Plant

Available Since

Nov. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pTUU

Plasmid

#159749

Purpose

Ubiquitin promoter-controlled expression of 3×FLAG-NLS-zCas9-NLS. Contains gRNA scaffold for insertion of target sequence (U6-26 promoter). Selection of transgenic seeds by mTomato fluoresce.

Depositor

Wolfgang Lukowitz

Article

Angulo et al bioRxiv

Insert

gRNA scaffold

Use

CRISPR

Expression

Plant

Available Since

Oct. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pICSL90016

Plasmid

#117514

Purpose

Cas9_2 Golden Gate Level 0

Depositor

Jonathan D Jones

Article

Castel et al PLoS One. 2019 Jan 9;14(1):e0204778. doi

Insert

BsaI:AATG:Cas9_2:GCTT:BsaI

Use

CRISPR

Tags

NLS_SV40

Expression

Plant

Available Since

Nov. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pCAMBIA-FLS2prom::FLS2-3xmyc

Plasmid

#86158

Purpose

FLS2 expression

Depositor

Silke Robatzek

Article

Robatzek et al Genes Dev. 2006 Mar 1;20(5):537-42.

Insert

FLS2 (FLS2 Mustard Weed)

Tags

3xmyc

Expression

Plant

Available Since

Feb. 8, 2017

Availability

Academic Institutions and Nonprofits only

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pMDC32B-AtMIR390a-NbSu-2

Plasmid

#213400

Purpose

Plant expression vector (2x35S) for expressing an amiRNA against Nicotiana benthamiana SULFUR gene from AtMIR390a precursor

Depositor

Alberto Carbonell

Article

Cisneros et al Plant J. 2022 May;110(4):1166-1181. doi:

Insert

Arabidopsis MIR390a precursor including amiRNA/amiRNA* sequences for silencing N. benthamiana SULFUR gene

Expression

Plant

Promoter

2x35S

Available Since

Feb. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pYPQ230-RV

Plasmid

#188544

Purpose

Gateway entry clone (attL1 & attR5) for LbCas12a-RV mediated genome editing

Depositor

Yiping Qi

Article

Zhang et al Genome Biol. 2023 Apr 30;24(1):102. doi:

Insert

LbCas12a-RV

Use

CRISPR; Gateway compatible lbcas12a-rv entry clone

Tags

NLS

Expression

Plant

Mutation

G146R, R182V

Available Since

May 17, 2023

Availability

Academic Institutions and Nonprofits only

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LLP743_L2pV1_OR_B3RT_FRT_Rluc_0I_lacZ

Plasmid

#192387

Purpose

To act as the 0-input state of the OR gate.

Depositor

Ryan Lister

Article

Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

Insert

Act2::FRT-B3RT-OCS-FRT-B3RT::Rluc

Use

Synthetic Biology

Tags

PEST

Expression

Plant

Available Since

Nov. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pGREAT18

Plasmid

#170906

Purpose

GoldenBraid Double Transcriptional Unit - pAtUBQ10_E-Luc_AtHSP18.2t+pNOS_Red-F_NOSt

Depositor

Markita Landry

Article

Gonzalez-Grandio et al ACS Synth Biol. 2021 Sep 14. doi: 10.102

Insert

E-Luc

Use

Luciferase and Synthetic Biology

Expression

Plant

Mutation

c.375C>T silent mutation to remove BbsI site (…

Promoter

pAtUBQ10

Available Since

Aug. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pFGC5941-PacI-ath-STTM858a/b

Plasmid

#84177

Purpose

target miRNA858 for destruction in Arabidopsis thaliana

Depositor

Guiliang Tang

Article

Tang et al Methods. 2012 Oct;58(2):118-25. doi: 10.

Insert

STTM858a/b

Expression

Plant

Promoter

2x35S

Available Since

Dec. 2, 2016

Availability

Academic Institutions and Nonprofits only

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