1,779 results

1. pSF11-wNIR-GECO2-T2A-HO1

   Plasmid

   #159606

   Purpose

   Codon-optimized near-infrared fluorescent calcium indicator NIR-GECO2 with codon-optimized heme-oxygenase in C.elegans expression plasmid

   Depositor

   Robert Campbell

   Article

   Qian et al PLoS Biol. 2020 Nov 24;18(11):e3000965.

   Insert

   wNIR-GECO2-T2A-HO1
   

   Expression

   Worm

   Promoter

   tag-168

   Available Since

   Sept. 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDD317 (Pmyo-2::GFP + SEC)

   Plasmid

   #73344

   Purpose

   SEC vector carry Pmyo-2::GFP and ccdB sites for cloning homology arms

   Depositor

   Bob Goldstein

   Article

   Hibshman et al BMC Biol. 2021 Dec 14;19(1):263. doi: 10

   Insert

   Pmyo-2::GFP + SEC
   

   Use

   CRISPR and Cre/Lox

   Tags

   GFP

   Expression

   Worm

   Promoter

   myo-2

   Available Since

   March 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAS1-rab3P::his-24::mNeptune

   Plasmid

   #124350

   Purpose

   Nuclear Localized Pan-Neuronal mNeptune

   Depositor

   Andrew Leifer

   Insert

   rab-3, mNeptune (rab-3 Nematode)
   

   Expression

   Worm

   Available Since

   April 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCFJ1410 - [1-2] ENTR - Fluor - mCherry(PATCs, NLS(2), no_atg, no_stop)

   Plasmid

   #159859

   Purpose

   Three-fragment Gateway compatible flourophore for expression in C. elegans

   Depositor

   Christian Froekjaer-Jensen

   Article

   Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

   Insert

   [1-2] ENTR - Fluor - mCherry(PATCs, NLS(2), no_atg, no_stop)
   

   Expression

   Worm

   Mutation

   Not applicable

   Available Since

   Dec. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pPD96_32

   Plasmid

   #1504

   Depositor

   Andrew Fire

   Type

   Empty backbone

   Tags

   IVSA, ATG-MitLS, gfpN, IVSR, gfpS65C, IVSS, IVST,…

   Expression

   Worm

   Available Since

   March 7, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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6. Pmec-18 ced-3 (p15)::nz [TU#806]

   Plasmid

   #16080

   Depositor

   Martin Chalfie

   Article

   Chelur et al Proc Natl Acad Sci U S A. 2007 Feb 13. 1

   Insert

   ced-3 (p15) :: nz (ced-3 Nematode)
   

   Expression

   Worm

   Available Since

   June 23, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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7. TC374

   Plasmid

   #122614

   Purpose

   Prpl-28::rtetR-QFAD::P2A::mKate::T2A::tetR-pie1

   Depositor

   Tian Chi

   Article

   Mao et al iScience. 2019 Jan 25;11:224-237. doi: 1

   Insert

   rtetR-QFAD::P2A::mKate::T2A::tetR-pie1
   

   Expression

   Worm

   Available Since

   May 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pPD49_83

   Plasmid

   #1448

   Depositor

   Andrew Fire

   Type

   Empty backbone

   Tags

   hsp16-25', hsp16-415', IVSA, rf2128

   Expression

   Worm

   Available Since

   March 7, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGM32DEST

   Plasmid

   #74747

   Purpose

   Encodes for the QF-GR recombinant gene, splice linker, and mCherry reporter gene

   Depositor

   Keith Yamamoto, Jordan Ward

   Article

   Monsalve et al Genetics. 2019 Feb;211(2):419-430. doi:

   Inserts

   Gateway cassette
   QF-GR (NR3C1 Neurospora crassa, Human, Synthetic)
   

   Tags

   Fusion of the Neurospora crassa QF activator to t…

   Expression

   Worm

   Promoter

   No promoter

   Available Since

   Oct. 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSLGCV

    Plasmid

    #49862

    Purpose

    Expression in Caenorhabditis elegans of the luc+ gene (Promega), fused in frame to GFP (S65C), under the sur-5 promoter (from plasmid pTG96, John Yochem and Min Han). Backbone pPD95.79 (Firelab).

    Depositor

    Jonathan Pettitt, Anne Glover

    Article

    Lagido et al BMC Physiol. 2008 Apr 2;8:7. doi: 10.118

    Insert

    firefly luciferase luc+ fused to GFP (S65C)
    

    Tags

    GFP (S65C)

    Expression

    Worm

    Promoter

    sur-5

    Available Since

    April 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. Pmec-18 cz::ced-3 (p17) [TU#807]

    Plasmid

    #16081

    Depositor

    Martin Chalfie

    Article

    Chelur et al Proc Natl Acad Sci U S A. 2007 Feb 13. 1

    Insert

    cz:: ced-3 (p17) (ced-3 Nematode)
    

    Expression

    Worm

    Available Since

    June 23, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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12. pNM3725

    Plasmid

    #154113

    Purpose

    UAS 11X ∆pes-10 promoter driving GFP-C1 in an RCME vector

    Depositor

    Michael Nonet

    Article

    Nonet et al Genetics. 2020 Jun 8. pii: genetics.120.

    Insert

    UAS 11X::∆pes-10::GFP-C1::tbb-2 3'
    

    Use

    Rmce

    Expression

    Worm

    Promoter

    not applicable

    Available Since

    July 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAV1825

    Plasmid

    #37838

    Depositor

    Gary Silverman

    Article

    Miedel et al PLoS One. 2012;7(7):e40145. Epub 2012 Ju

    Insert

    Pnhx-2DsRed::KDEL
    

    Tags

    KDEL

    Expression

    Worm

    Promoter

    nhx-2

    Available Since

    Dec. 5, 2012

    Availability

    Academic Institutions and Nonprofits only

14. pHT101-mCherry

    Plasmid

    #61021

    Purpose

    Expresses mCherry tagged fusion proteins in worms

    Depositor

    Casonya Johnson

    Type

    Empty backbone

    Tags

    mCherry

    Expression

    Worm

    Promoter

    lac

    Available Since

    Nov. 21, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. pJW2172

    Plasmid

    #163096

    Purpose

    CRISPR repair template: add insertion site specific homology arms by PCR before insertion

    Depositor

    Jordan Ward

    Article

    Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

    Insert

    30aa linker::mNeonGreen (dpi)::3xFLAG::10aa linker
    

    Use

    CRISPR

    Expression

    Worm

    Mutation

    Silent mutations to remove piRNA sites

    Available Since

    Jan. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSEM388 - mosTI unc-119 - NNN - mScarlet - tbb-2

    Plasmid

    #182348

    Purpose

    mScarlet fusion cloning vector for mosTI(unc-119). Transgene inserted upstream of mScarlet and tbb-2 3' UTR. MCS or Golden Gate (BsaI: tgcc - MCS - aaaa)

    Depositor

    Christian Froekjaer-Jensen

    Article

    El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

    Type

    Empty backbone

    Expression

    Worm

    Available Since

    May 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pJW1586

    Plasmid

    #121057

    Purpose

    mKate2^SEC^AID*::3xFLAG vector with ccdB sites for cloning homology arms.

    Depositor

    Jordan Ward

    Article

    Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

    Insert

    mKate2-T^SEC^AID*::3xFlag
    

    Use

    CRISPR and Cre/Lox

    Tags

    3xFLAG, C. elegans codon optimized mKate2, and au…

    Expression

    Worm

    Available Since

    June 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pPD49_78

    Plasmid

    #1447

    Depositor

    Andrew Fire

    Type

    Empty backbone

    Tags

    hsp16-415', hsp16-25', IVSA, rf2128

    Expression

    Worm

    Available Since

    March 7, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDSP16 (5’Δ Cbr-unc-119(+) + loxN + MCS + 5’Δ mNeonGreen)

    Plasmid

    #193854

    Purpose

    Promoterless insertion vector for split Cbr-unc-19(+)/mNeonGreen landing pad

    Depositor

    Patrick Phillips

    Article

    Stevenson et al Elife. 2023 Jul 4;12:RP84831. doi: 10.75

    Insert

    5' Cbr-unc-119(+) + 5' mNeonGreen
    

    Use

    CRISPR and Cre/Lox

    Expression

    Worm

    Promoter

    Cbr-unc-119p

    Available Since

    Sept. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pJH1579

    Plasmid

    #178904

    Purpose

    Pglr-1::YC3.60 unc-54 3' UTR C.elegans neural expression of Pglr-1 YC3.60

    Depositor

    Mei Zhen

    Article

    Kawano et al Neuron. 2011 Nov 17;72(4):572-86. doi: 1

    Insert

    YC3.60
    

    Expression

    Worm

    Mutation

    See Depositor Comments Below

    Promoter

    Pglr-1

    Available Since

    Jan. 6, 2022

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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